WO2002004122A3 - Improved stability of hybridisation interactions in dipstick assays - Google Patents

Improved stability of hybridisation interactions in dipstick assays Download PDF

Info

Publication number
WO2002004122A3
WO2002004122A3 PCT/GB2001/003047 GB0103047W WO0204122A3 WO 2002004122 A3 WO2002004122 A3 WO 2002004122A3 GB 0103047 W GB0103047 W GB 0103047W WO 0204122 A3 WO0204122 A3 WO 0204122A3
Authority
WO
WIPO (PCT)
Prior art keywords
hybridisation
interactions
improved stability
nucleic acid
dipstick assays
Prior art date
Application number
PCT/GB2001/003047
Other languages
French (fr)
Other versions
WO2002004122B1 (en
WO2002004122A2 (en
Inventor
Helen Lee
Magda Anastassova Dineva
Original Assignee
Helen Lee
Magda Anastassova Dineva
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0016836.9A external-priority patent/GB0016836D0/en
Priority claimed from GB0016812A external-priority patent/GB0016812D0/en
Application filed by Helen Lee, Magda Anastassova Dineva filed Critical Helen Lee
Priority to AU2001269292A priority Critical patent/AU2001269292A1/en
Publication of WO2002004122A2 publication Critical patent/WO2002004122A2/en
Publication of WO2002004122A3 publication Critical patent/WO2002004122A3/en
Publication of WO2002004122B1 publication Critical patent/WO2002004122B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Abstract

Use of probes with non complementary nucleotides in dipstick assays to test for the presence of a target nucleic acid in a sample solution is described. The probes have greater affinity for target nucleic acid and can be used in capture and/or detection of target nucleic acid. The sensitivity of target nucleic acid detection is thereby increased. Dipsticks and kits are also described.
PCT/GB2001/003047 2000-07-07 2001-07-06 Improved stability of hybridisation interactions in dipstick assays WO2002004122A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001269292A AU2001269292A1 (en) 2000-07-07 2001-07-06 Improved stability of hybridisation interactions in dipstick assays

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0016836.9A GB0016836D0 (en) 2000-07-07 2000-07-07 Improved dipstick assays (1)
GB0016812A GB0016812D0 (en) 2000-07-07 2000-07-07 Enhancing nucleic acid hybridisation
GB0016836.9 2000-07-07
GB0016812.0 2000-07-07

Publications (3)

Publication Number Publication Date
WO2002004122A2 WO2002004122A2 (en) 2002-01-17
WO2002004122A3 true WO2002004122A3 (en) 2002-12-27
WO2002004122B1 WO2002004122B1 (en) 2003-08-07

Family

ID=26244625

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/003047 WO2002004122A2 (en) 2000-07-07 2001-07-06 Improved stability of hybridisation interactions in dipstick assays

Country Status (3)

Country Link
AU (1) AU2001269292A1 (en)
TW (1) TWI286159B (en)
WO (1) WO2002004122A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8669052B2 (en) 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
US20110086359A1 (en) 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
EP2313527A4 (en) * 2008-07-15 2012-11-21 Rapid Pathogen Screening Inc Lateral flow nucleic acid detector
CA2775273A1 (en) * 2009-10-22 2011-04-28 Biotools Biotechnological & Medical Laboratories, S.A. Composition, method and kit for detecting bacteria by means of sequencing
CN102154498B (en) * 2011-03-21 2013-12-04 厦门大学 Nucleic acid detecting method
CA2938817A1 (en) * 2014-02-05 2015-08-13 Fuso Pharmaceutical Industries, Ltd. Nucleic acid detection or quantification method using mask oligonucleotide, and device for same

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0198662A1 (en) * 1985-04-12 1986-10-22 AMERSHAM INTERNATIONAL plc Method for nucleic acid hybridisation
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
WO1990001564A1 (en) * 1988-08-09 1990-02-22 Microprobe Corporation Methods for multiple target analyses through nucleic acid hybridization
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
WO1994023299A1 (en) * 1993-03-31 1994-10-13 Quidel Corporation Multiple assay device
WO1994029696A1 (en) * 1993-06-09 1994-12-22 Quidel Corporation Antigen-specific one-step assays
WO1995027081A1 (en) * 1994-03-31 1995-10-12 E.I. Du Pont De Nemours And Company A method for detection of nucleic acid fragments

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0198662A1 (en) * 1985-04-12 1986-10-22 AMERSHAM INTERNATIONAL plc Method for nucleic acid hybridisation
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
WO1990001564A1 (en) * 1988-08-09 1990-02-22 Microprobe Corporation Methods for multiple target analyses through nucleic acid hybridization
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
WO1994023299A1 (en) * 1993-03-31 1994-10-13 Quidel Corporation Multiple assay device
WO1994029696A1 (en) * 1993-06-09 1994-12-22 Quidel Corporation Antigen-specific one-step assays
WO1995027081A1 (en) * 1994-03-31 1995-10-12 E.I. Du Pont De Nemours And Company A method for detection of nucleic acid fragments

Also Published As

Publication number Publication date
TWI286159B (en) 2007-09-01
AU2001269292A1 (en) 2002-01-21
WO2002004122B1 (en) 2003-08-07
WO2002004122A2 (en) 2002-01-17

Similar Documents

Publication Publication Date Title
WO2002004668A3 (en) Improved capture and detection of target nucleic acid in dipstick assays
SE0000538D0 (en) Methods and kits for proximity probing
BR0015582A (en) Immunological detection of rna: dna hybrids in microarrays
ATE403153T1 (en) TEST FOR DIRECT DETECTION OF A RS VIRUS RELATED BIOLOGICAL CELL IN A BODY FLUID SAMPLE
DK244889D0 (en) PROCEDURE FOR DETERMINING AN ANALYST
WO2003058242A3 (en) Internal calibration system for flow-through assays
EP0805215A3 (en) Diagnostic methods and probes
NZ306051A (en) Testing using electrochemiluminescence
EP0360088A3 (en) Indicator reagents, diagnostic assays and test kits employing organic polymer latex particles
ES8507181A1 (en) Method of measuring polynucleotide and reagent kit for use therein.
ATE515693T1 (en) DETECTION OF A TARGET SUBSTANCE IN A SAMPLE
WO2002004669A3 (en) Improved capture and detection format versatility for dipstick assays
WO1998017829A3 (en) Nucleic acid primers and probes for detecting oncogenic human papillomaviruses
BR0107831A (en) Homogeneous duplex or triplex hybridization assay by multiple measurements under varying conditions
WO2005073405A3 (en) Catalytic polynucleotide and its use for determination of analytes
WO2002004671A3 (en) Improved binding interactions in dipstick assays
WO2002004667A3 (en) Improved detection signal and capture in dipstick assays
WO2002004122A3 (en) Improved stability of hybridisation interactions in dipstick assays
WO1997046709A3 (en) NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(CHLAMYDIA PNEUMONIAE)
WO2007001991A3 (en) Detection of adulterated samples
HK1091540A1 (en) Use of a virus expressing a binding moiety to measure analytes in a sample
DE69935850D1 (en) METHOD FOR MEASURING THE OVERALL CONCENTRATION OF AN ANALYTE
CA2145957A1 (en) Method, test element and kit for semi-quantitative detection of target nucleic acid
WO2002018952A3 (en) Biosensor assay device and method
WO1998000565A3 (en) NUCLEIC ACID PRIMERS AND PROBES FOR DETECTING $i(MYCOPLASMA PNEUMONIAE)

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

B Later publication of amended claims

Free format text: 20021210

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP