WO2002004680A3 - Real-time sequence determination - Google Patents

Real-time sequence determination Download PDF

Info

Publication number
WO2002004680A3
WO2002004680A3 PCT/US2001/021811 US0121811W WO0204680A3 WO 2002004680 A3 WO2002004680 A3 WO 2002004680A3 US 0121811 W US0121811 W US 0121811W WO 0204680 A3 WO0204680 A3 WO 0204680A3
Authority
WO
WIPO (PCT)
Prior art keywords
real
time sequence
sequence determination
methodology
dntps
Prior art date
Application number
PCT/US2001/021811
Other languages
French (fr)
Other versions
WO2002004680A2 (en
Inventor
Susan H Hardin
James M Briggs
Shiao-Chun Tu
Xiaolian Gao
Richard Willson
Original Assignee
Visigen Biotechnologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22807697&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2002004680(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Visigen Biotechnologies Inc filed Critical Visigen Biotechnologies Inc
Priority to DE60131194T priority Critical patent/DE60131194T2/en
Priority to EP01961632A priority patent/EP1368460B1/en
Priority to JP2002509533A priority patent/JP2004513619A/en
Priority to CA002415897A priority patent/CA2415897A1/en
Priority to AU2001282881A priority patent/AU2001282881B2/en
Priority to AU8288101A priority patent/AU8288101A/en
Publication of WO2002004680A2 publication Critical patent/WO2002004680A2/en
Publication of WO2002004680A3 publication Critical patent/WO2002004680A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing

Abstract

A sequencing methodology is disclosed that allows a single DNA or RNA molecule or portion thereof to be sequenced directly and in substantially real time. The methodology involves engineering a polymerase and/or dNTPs with atomic and/or molecular tags that have a detectable property that is monitored by a detection system.
PCT/US2001/021811 2000-07-07 2001-07-09 Real-time sequence determination WO2002004680A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE60131194T DE60131194T2 (en) 2000-07-07 2001-07-09 SEQUENCE PROVISION IN REAL TIME
EP01961632A EP1368460B1 (en) 2000-07-07 2001-07-09 Real-time sequence determination
JP2002509533A JP2004513619A (en) 2000-07-07 2001-07-09 Real-time sequencing
CA002415897A CA2415897A1 (en) 2000-07-07 2001-07-09 Real-time sequence determination
AU2001282881A AU2001282881B2 (en) 2000-07-07 2001-07-09 Real-time sequence determination
AU8288101A AU8288101A (en) 2000-07-07 2001-07-09 Real-time sequence determination

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US21659400P 2000-07-07 2000-07-07
US60/216,594 2000-07-07

Publications (2)

Publication Number Publication Date
WO2002004680A2 WO2002004680A2 (en) 2002-01-17
WO2002004680A3 true WO2002004680A3 (en) 2003-10-16

Family

ID=22807697

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/021811 WO2002004680A2 (en) 2000-07-07 2001-07-09 Real-time sequence determination

Country Status (9)

Country Link
US (22) US20030064366A1 (en)
EP (3) EP1368460B1 (en)
JP (3) JP2004513619A (en)
CN (2) CN101525660A (en)
AT (1) ATE377093T1 (en)
AU (2) AU2001282881B2 (en)
CA (1) CA2415897A1 (en)
DE (1) DE60131194T2 (en)
WO (1) WO2002004680A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9365838B2 (en) 2009-03-27 2016-06-14 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis

Families Citing this family (678)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9213691D0 (en) * 1992-06-27 1992-08-12 Hurlstone Gary Personal alarm torch
US5823720A (en) * 1996-02-16 1998-10-20 Bitmoore High precision cutting tools
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
CA2355816C (en) 1998-12-14 2007-10-30 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
DE60131194T2 (en) * 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire SEQUENCE PROVISION IN REAL TIME
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
WO2002072892A1 (en) * 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US7668697B2 (en) 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7727722B2 (en) * 2001-08-29 2010-06-01 General Electric Company Ligation amplification
ATE458067T1 (en) * 2001-08-29 2010-03-15 Ge Healthcare Bio Sciences LABELED NUCLEOSIDE POLYPHOSPHATES
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US20050009124A1 (en) * 2001-11-26 2005-01-13 Echelon Biosciences Incorporated Assays for detection of phosphoinositide kinase and phosphatase activity
EP1497304B1 (en) * 2002-04-12 2014-06-25 Catalyst Assets LLC Dual-labeled nucleotides
ATE547535T1 (en) * 2002-08-29 2012-03-15 Ge Healthcare Bio Sciences NUCLEOSIDE POLYPHOSPHATES WITH BLOCKED TERMINAL PHOSPHATE
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
EP2363500A1 (en) * 2002-11-14 2011-09-07 John Wayne Cancer Institute Detection of micro metastasis of melanoma and breast cancer in paraffin-embedded tumor draining lymph nodes by multimaker quantitative RT-PCR
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
WO2004072238A2 (en) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides with new linkers
ES2355555T3 (en) * 2003-02-05 2011-03-28 Ge Healthcare Bio-Sciences Corp. AMPLIFICATION OF NUCLEIC ACIDS.
WO2004071948A2 (en) * 2003-02-10 2004-08-26 Reveo, Inc. Micro-nozzle, nano-nozzle, manufacturing methods therefor, applications therefor
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US20060030037A1 (en) 2004-05-28 2006-02-09 Victor Joseph Thermo-controllable high-density chips for multiplex analyses
US7692219B1 (en) 2004-06-25 2010-04-06 University Of Hawaii Ultrasensitive biosensors
GB2423819B (en) * 2004-09-17 2008-02-06 Pacific Biosciences California Apparatus and method for analysis of molecules
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US7405434B2 (en) * 2004-11-16 2008-07-29 Cornell Research Foundation, Inc. Quantum dot conjugates in a sub-micrometer fluidic channel
JP2006197832A (en) * 2005-01-19 2006-08-03 Tohoku Univ Multi-purpose method for detecting substrate affinity of abc transporter having cysteine residue induced by variation by using environment-sensitive fluorescent probe
CA2596495A1 (en) * 2005-02-09 2006-08-17 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof
CN101218497B (en) * 2005-07-07 2012-04-25 索尼株式会社 Material information acquiring method and material information measuring device using evanescent light, and method and system for determination of base sequence
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007041621A2 (en) * 2005-10-03 2007-04-12 Xingsheng Sean Ling Hybridization assisted nanopore sequencing
US9156004B2 (en) 2005-10-17 2015-10-13 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US7825037B2 (en) * 2005-10-17 2010-11-02 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US10060904B1 (en) 2005-10-17 2018-08-28 Stc.Unm Fabrication of enclosed nanochannels using silica nanoparticles
US8017331B2 (en) * 2005-11-04 2011-09-13 Mannkind Corporation IRE-1α substrates
US8344121B2 (en) 2005-12-12 2013-01-01 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US20080241938A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Automated synthesis or sequencing apparatus and method for making and using same
US20080241951A1 (en) * 2006-07-20 2008-10-02 Visigen Biotechnologies, Inc. Method and apparatus for moving stage detection of single molecular events
US7897737B2 (en) 2006-12-05 2011-03-01 Lasergen, Inc. 3′-OH unblocked, nucleotides and nucleosides, base modified with photocleavable, terminating groups and methods for their use in DNA sequencing
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
JP5622392B2 (en) 2006-12-14 2014-11-12 ライフ テクノロジーズ コーポレーション Method and apparatus for analyte measurement using large-scale FET arrays
EP2126072A4 (en) * 2007-02-21 2011-07-13 Life Technologies Corp Materials and methods for single molecule nucleic acid sequencing
US9121843B2 (en) 2007-05-08 2015-09-01 Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
WO2008147879A1 (en) * 2007-05-22 2008-12-04 Ryan Golhar Automated method and device for dna isolation, sequence determination, and identification
WO2008151023A2 (en) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
US8703422B2 (en) 2007-06-06 2014-04-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
CA2689626C (en) * 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2009046094A1 (en) 2007-10-01 2009-04-09 Nabsys, Inc. Biopolymer sequencing by hybridization of probes to form ternary complexes and variable range alignment
WO2009055508A1 (en) * 2007-10-22 2009-04-30 Life Technologies Corporation A method and system for obtaining ordered, segmented sequence fragments along a nucleic acid molecule
GB0721340D0 (en) * 2007-10-30 2007-12-12 Isis Innovation Polymerase-based single-molecule DNA sequencing
WO2009117031A2 (en) * 2007-12-18 2009-09-24 Advanced Analytical Technologies, Inc. System and method for nucleotide sequence profiling for sample identification
CN101519698B (en) * 2008-03-10 2013-05-22 周国华 Method for quantitatively measuring nucleic acid with sequence tags
BRPI0909212A2 (en) 2008-03-28 2015-08-18 Pacific Biosciences California Compositions and method for nucleic acid sequencing
JP4586081B2 (en) * 2008-03-31 2010-11-24 株式会社日立ハイテクノロジーズ Fluorescence analyzer
US20090269746A1 (en) * 2008-04-25 2009-10-29 Gil Atzmon Microsequencer-whole genome sequencer
CA2727709C (en) 2008-06-11 2017-11-14 Lasergen, Inc. Nucleotides and nucleosides and methods for their use in dna sequencing
JP5268444B2 (en) * 2008-06-23 2013-08-21 株式会社日立ハイテクノロジーズ Single molecule real-time sequence device, nucleic acid analyzer, and single molecule real-time sequence method
WO2010008480A2 (en) 2008-06-25 2010-01-21 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
WO2010002939A2 (en) * 2008-06-30 2010-01-07 Life Technologies Corporation Methods for real time single molecule sequencing
US20100036110A1 (en) * 2008-08-08 2010-02-11 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US20100035252A1 (en) 2008-08-08 2010-02-11 Ion Torrent Systems Incorporated Methods for sequencing individual nucleic acids under tension
US20100227327A1 (en) * 2008-08-08 2010-09-09 Xiaoliang Sunney Xie Methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides
US8262879B2 (en) 2008-09-03 2012-09-11 Nabsys, Inc. Devices and methods for determining the length of biopolymers and distances between probes bound thereto
JP5717634B2 (en) * 2008-09-03 2015-05-13 ナブシス, インコーポレイテッド Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluid channels
US9650668B2 (en) 2008-09-03 2017-05-16 Nabsys 2.0 Llc Use of longitudinally displaced nanoscale electrodes for voltage sensing of biomolecules and other analytes in fluidic channels
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20100184045A1 (en) * 2008-09-23 2010-07-22 Helicos Biosciences Corporation Methods for sequencing degraded or modified nucleic acids
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
CA2742298C (en) 2008-11-03 2019-09-10 The Regents Of The University Of California Methods for detecting modification resistant nucleic acids
US8236532B2 (en) 2008-12-23 2012-08-07 Illumina, Inc. Multibase delivery for long reads in sequencing by synthesis protocols
US9778188B2 (en) 2009-03-11 2017-10-03 Industrial Technology Research Institute Apparatus and method for detection and discrimination molecular object
US8455260B2 (en) * 2009-03-27 2013-06-04 Massachusetts Institute Of Technology Tagged-fragment map assembly
WO2010111605A2 (en) * 2009-03-27 2010-09-30 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
CA2760155A1 (en) * 2009-04-27 2010-11-11 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
EP2427572B1 (en) * 2009-05-01 2013-08-28 Illumina, Inc. Sequencing methods
US8246799B2 (en) * 2009-05-28 2012-08-21 Nabsys, Inc. Devices and methods for analyzing biomolecules and probes bound thereto
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US8632975B2 (en) 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
US8609421B2 (en) * 2009-06-12 2013-12-17 Pacific Biosciences Of California, Inc. Single-molecule real-time analysis of protein synthesis
WO2011014811A1 (en) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Capture primers and capture sequence linked solid supports for molecular diagnostic tests
CN102625838B (en) 2009-08-14 2016-01-20 阿霹震中科技公司 For generating sample that rRNA eliminates or for from the method for sample separation rRNA, composition and test kit
WO2011028296A2 (en) * 2009-09-07 2011-03-10 Caerus Molecular Diagnostics Incorporated Sequence determination by use of opposing forces
WO2011078897A1 (en) * 2009-09-15 2011-06-30 Life Technologies Corporation Improved sequencing methods, compositions, systems, kits and apparatuses
EP2483680A4 (en) 2009-09-30 2014-01-01 Quantapore Inc Ultrafast sequencing of biological polymers using a labeled nanopore
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
CN102741430B (en) 2009-12-01 2016-07-13 牛津楠路珀尔科技有限公司 Biochemical analyzer, for first module carrying out biochemical analysis and associated method
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US9315857B2 (en) 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags
US8911972B2 (en) 2009-12-16 2014-12-16 Pacific Biosciences Of California, Inc. Sequencing methods using enzyme conformation
EP2519823B1 (en) * 2009-12-28 2022-05-18 The United States Of America, As Represented By The Secretary, Department of Health and Human Services Composite probes and use thereof in super resolution methods
EP3151052A1 (en) 2010-02-01 2017-04-05 Illumina, Inc. Focusing methods and optical systems and assemblies using the same
US8603741B2 (en) 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
CN202281746U (en) 2010-03-06 2012-06-20 伊鲁米那股份有限公司 Measuring equipment for detecting optical signal from sample as well as optical module and optical system for measuring equipment
US9482615B2 (en) 2010-03-15 2016-11-01 Industrial Technology Research Institute Single-molecule detection system and methods
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
WO2011127099A1 (en) 2010-04-05 2011-10-13 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
CA2798635A1 (en) * 2010-05-06 2011-11-10 Ibis Biosciences, Inc. Integrated sample preparation systems and stabilized enzyme mixtures
US9670243B2 (en) 2010-06-02 2017-06-06 Industrial Technology Research Institute Compositions and methods for sequencing nucleic acids
US8865077B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US8865078B2 (en) 2010-06-11 2014-10-21 Industrial Technology Research Institute Apparatus for single-molecule detection
US9353412B2 (en) 2010-06-18 2016-05-31 Illumina, Inc. Conformational probes and methods for sequencing nucleic acids
US9164070B2 (en) 2010-06-30 2015-10-20 Life Technologies Corporation Column adc
JP2013533482A (en) 2010-06-30 2013-08-22 ライフ テクノロジーズ コーポレーション Ion-sensitive charge storage circuit and method
US20120001646A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Methods and apparatus for testing isfet arrays
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
JP5876044B2 (en) 2010-07-03 2016-03-02 ライフ テクノロジーズ コーポレーション Chemically sensitive sensor with lightly doped drain
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
WO2012027623A2 (en) 2010-08-25 2012-03-01 Pacific Biosciences Of California, Inc. Cyanine dyes
EP2617061B1 (en) 2010-09-15 2021-06-30 Life Technologies Corporation Methods and apparatus for measuring analytes
EP3327140A1 (en) 2010-09-16 2018-05-30 Gen-Probe Incorporated Capture probes immobilizable via l-nucleotide tail
AU2011301935B2 (en) 2010-09-16 2015-06-11 Ibis Biosciences, Inc. Stabilization of ozone-labile fluorescent dyes by thiourea
CN105911126B (en) 2010-09-24 2018-12-18 生命科技公司 Matched transistor pair circuit
US8715933B2 (en) 2010-09-27 2014-05-06 Nabsys, Inc. Assay methods using nicking endonucleases
EP2633069B1 (en) 2010-10-26 2015-07-01 Illumina, Inc. Sequencing methods
US9255293B2 (en) 2010-11-01 2016-02-09 Gen-Probe Incorporated Integrated capture and amplification of target nucleic acid for sequencing
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012067911A1 (en) 2010-11-16 2012-05-24 Nabsys, Inc. Methods for sequencing a biomolecule by detecting relative positions of hybridized probes
EP2643484A4 (en) 2010-11-22 2014-04-16 Univ California Methods of identifying a cellular nascent rna transcript
EP2658988B1 (en) 2010-12-27 2018-01-24 Ibis Biosciences, Inc. Nucleic acid sample preparation methods and compositions
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US20130316358A1 (en) 2011-01-31 2013-11-28 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
WO2012106081A2 (en) 2011-01-31 2012-08-09 Illumina, Inc. Methods for reducing nucleic acid damage
JP6017458B2 (en) 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション Mass parallel continuity mapping
WO2012109315A1 (en) 2011-02-08 2012-08-16 Life Technologies Corporation Linking methods, compositions, systems, kits and apparatuses
US11274341B2 (en) 2011-02-11 2022-03-15 NABsys, 2.0 LLC Assay methods using DNA binding proteins
ES2538694T3 (en) 2011-04-01 2015-06-23 F. Hoffmann-La Roche Ag Variants of the T7 RNA polymerase with Cysteine-Serine substitutions
US20120258871A1 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP2718465B1 (en) 2011-06-09 2022-04-13 Illumina, Inc. Method of making an analyte array
GB201113430D0 (en) 2011-08-03 2011-09-21 Fermentas Uab DNA polymerases
US9670538B2 (en) 2011-08-05 2017-06-06 Ibis Biosciences, Inc. Nucleic acid sequencing by electrochemical detection
US11208636B2 (en) 2011-08-10 2021-12-28 Life Technologies Corporation Polymerase compositions, methods of making and using same
EP3293274B1 (en) 2011-08-10 2020-04-01 Life Technologies Corporation Polymerase compositions
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
US8889860B2 (en) 2011-09-13 2014-11-18 Lasergen, Inc. 3′-OH unblocked, fast photocleavable terminating nucleotides and methods for nucleic acid sequencing
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
US9164053B2 (en) * 2011-09-26 2015-10-20 The Regents Of The University Of California Electronic device for monitoring single molecule dynamics
WO2013063308A1 (en) 2011-10-25 2013-05-02 University Of Massachusetts An enzymatic method to enrich for capped rna, kits for performing same, and compositions derived therefrom
EP2771103B1 (en) 2011-10-28 2017-08-16 Illumina, Inc. Microarray fabrication system and method
JP5863396B2 (en) * 2011-11-04 2016-02-16 株式会社日立製作所 DNA sequence decoding system, DNA sequence decoding method and program
CA2854023A1 (en) 2011-11-07 2013-05-16 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
DK2788499T3 (en) 2011-12-09 2016-03-21 Illumina Inc Enhanced root for polymer tags
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
EP3269820A1 (en) 2011-12-22 2018-01-17 Ibis Biosciences, Inc. Kit for the amplification of a sequence from a ribonucleic acid
WO2013096799A1 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Systems and methods for isolating nucleic acids from cellular samples
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
EP2794927B1 (en) 2011-12-22 2017-04-12 Ibis Biosciences, Inc. Amplification primers and methods
US9506113B2 (en) 2011-12-28 2016-11-29 Ibis Biosciences, Inc. Nucleic acid ligation systems and methods
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
US9222115B2 (en) 2011-12-30 2015-12-29 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
CA2860594A1 (en) 2012-01-09 2013-07-18 Oslo Universitetssykehus Hf Methods and biomarkers for analysis of colorectal cancer
CN104334739A (en) 2012-01-13 2015-02-04 Data生物有限公司 Genotyping by next-generation sequencing
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
CA2863084C (en) 2012-02-01 2020-07-07 Gen-Probe Incorporated Asymmetric hairpin target capture oligomers
EP4361609A2 (en) * 2012-02-03 2024-05-01 California Institute of Technology Signal encoding and decoding in multiplexed biochemical assays
US11177020B2 (en) 2012-02-27 2021-11-16 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
NO2694769T3 (en) 2012-03-06 2018-03-03
US20130261984A1 (en) 2012-03-30 2013-10-03 Illumina, Inc. Methods and systems for determining fetal chromosomal abnormalities
WO2013152114A1 (en) 2012-04-03 2013-10-10 The Regents Of The University Of Michigan Biomarker associated with irritable bowel syndrome and crohn's disease
US9193996B2 (en) 2012-04-03 2015-11-24 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
US20130274148A1 (en) 2012-04-11 2013-10-17 Illumina, Inc. Portable genetic detection and analysis system and method
ES2840456T3 (en) 2012-05-02 2021-07-06 Ibis Biosciences Inc DNA sequencing
ES2683707T3 (en) 2012-05-02 2018-09-27 Ibis Biosciences, Inc. DNA sequencing
WO2013166304A1 (en) 2012-05-02 2013-11-07 Ibis Biosciences, Inc. Dna sequencing
US20150133310A1 (en) 2012-05-02 2015-05-14 Ibis Biosciences, Inc. Nucleic acid sequencing systems and methods
WO2013173844A1 (en) 2012-05-18 2013-11-21 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes
US9315864B2 (en) 2012-05-18 2016-04-19 Pacific Biosciences Of California, Inc. Heteroarylcyanine dyes with sulfonic acid substituents
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9012022B2 (en) 2012-06-08 2015-04-21 Illumina, Inc. Polymer coatings
US8895249B2 (en) 2012-06-15 2014-11-25 Illumina, Inc. Kinetic exclusion amplification of nucleic acid libraries
WO2014005076A2 (en) 2012-06-29 2014-01-03 The Regents Of The University Of Michigan Methods and biomarkers for detection of kidney disorders
EP2870264A4 (en) 2012-07-03 2016-03-02 Sloan Kettering Inst Cancer Quantitative assessment of human t-cell repertoire recovery after allogeneic hematopoietic stem cell transplantation
EP2875156A4 (en) 2012-07-19 2016-02-24 Ariosa Diagnostics Inc Multiplexed sequential ligation-based detection of genetic variants
NL2017959B1 (en) 2016-12-08 2018-06-19 Illumina Inc Cartridge assembly
US10895534B2 (en) 2012-08-20 2021-01-19 Illumina, Inc. Method and system for fluorescence lifetime based sequencing
WO2014062835A1 (en) 2012-10-16 2014-04-24 Abbott Molecular Inc. Methods and apparatus to sequence a nucleic acid
US9181583B2 (en) 2012-10-23 2015-11-10 Illumina, Inc. HLA typing using selective amplification and sequencing
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9605309B2 (en) * 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9914966B1 (en) 2012-12-20 2018-03-13 Nabsys 2.0 Llc Apparatus and methods for analysis of biomolecules using high frequency alternating current excitation
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014113557A1 (en) 2013-01-18 2014-07-24 Nabsys, Inc. Enhanced probe binding
US9805407B2 (en) 2013-01-25 2017-10-31 Illumina, Inc. Methods and systems for using a cloud computing environment to configure and sell a biological sample preparation cartridge and share related data
US9512422B2 (en) 2013-02-26 2016-12-06 Illumina, Inc. Gel patterned surfaces
CA2898453C (en) 2013-03-13 2021-07-27 Illumina, Inc. Multilayer fluidic devices and methods for their fabrication
EP3919617A1 (en) 2013-03-13 2021-12-08 Illumina, Inc. Methods and compositions for nucleic acid sequencing
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
JP2016512437A (en) 2013-03-14 2016-04-28 アボツト・モレキユラー・インコーポレイテツド Multiple methylation specific amplification systems and methods
CA2898459C (en) 2013-03-14 2021-02-02 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
EP2971154A4 (en) 2013-03-14 2017-03-01 Ibis Biosciences, Inc. Nucleic acid control panels
WO2014149780A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
CN105358709B (en) 2013-03-15 2018-12-07 雅培分子公司 System and method for detecting genome copy numbers variation
EP3388442A1 (en) 2013-03-15 2018-10-17 Illumina Cambridge Limited Modified nucleosides or nucleotides
EP2971140B1 (en) 2013-03-15 2019-01-16 Ibis Biosciences, Inc. Methods to assess contamination in dna sequencing
US20140274747A1 (en) 2013-03-15 2014-09-18 Illumina, Inc. Super resolution imaging
US9193998B2 (en) 2013-03-15 2015-11-24 Illumina, Inc. Super resolution imaging
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
CN105051525B (en) 2013-03-15 2019-07-26 生命科技公司 Chemical device with thin conducting element
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
CA2910019A1 (en) 2013-05-24 2014-11-27 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed fret detection
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
WO2014210225A1 (en) 2013-06-25 2014-12-31 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
ES2700529T3 (en) 2013-07-01 2019-02-18 Illumina Inc Functionalization of surface without catalyst and polymer graft
ES2719579T3 (en) 2013-07-03 2019-07-11 Illumina Inc System for sequencing by orthogonal synthesis
CN103333680B (en) * 2013-07-16 2015-05-27 北京化工大学 Dinyl oxazole eutectic material with multi-color fluorescence characteristic and preparation method of dinyl oxazole eutectic material
US9410977B2 (en) 2013-08-08 2016-08-09 Illumina, Inc. Fluidic system for reagent delivery to a flow cell
ES2873850T3 (en) 2013-08-19 2021-11-04 Abbott Molecular Inc Next Generation Sequencing Libraries
AU2014312208B2 (en) 2013-08-28 2019-07-25 Becton, Dickinson And Company Massively parallel single cell analysis
AU2014312043A1 (en) 2013-08-30 2016-02-25 Illumina France Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
EP3049539B1 (en) 2013-09-25 2018-09-05 Bio-Id Diagnostic Inc. Methods for detecting nucleic acid fragments
EP3052652B1 (en) 2013-09-30 2022-11-23 Life Technologies Corporation Polymerase compositions, methods of making and using same
WO2015054292A1 (en) 2013-10-07 2015-04-16 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
US10540783B2 (en) * 2013-11-01 2020-01-21 Illumina, Inc. Image analysis useful for patterned objects
EP3715467A1 (en) 2013-12-03 2020-09-30 Illumina, Inc. Methods and systems for analyzing image data
EP3080585B1 (en) 2013-12-10 2023-12-06 Illumina, Inc. Biosensors for biological or chemical analysis and methods of manufacturing the same
WO2015095355A2 (en) 2013-12-17 2015-06-25 The Brigham And Women's Hospital, Inc. Detection of an antibody against a pathogen
EP3083994B1 (en) 2013-12-20 2021-08-18 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic dna samples
AU2014369879B2 (en) 2013-12-23 2020-12-24 Illumina, Inc. Structured substrates for improving detection of light emissions and methods relating to the same
US9677132B2 (en) 2014-01-16 2017-06-13 Illumina, Inc. Polynucleotide modification on solid support
WO2015107430A2 (en) 2014-01-16 2015-07-23 Oslo Universitetssykehus Hf Methods and biomarkers for detection and prognosis of cervical cancer
CN112322735A (en) 2014-01-16 2021-02-05 启迪公司 Gene expression panels for prognosis of prostate cancer recurrence
US9926598B2 (en) 2014-01-16 2018-03-27 Illumina, Inc. Amplicon preparation and sequencing on solid supports
NZ723399A (en) 2014-02-18 2020-08-28 Illumina Inc Methods and compositions for dna profiling
CN106459869B (en) 2014-03-11 2020-01-21 伊鲁米那股份有限公司 Disposable integrated microfluidic cartridge and methods of making and using same
CA2947426C (en) 2014-04-29 2020-01-07 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
SG11201610168YA (en) 2014-05-16 2017-01-27 Illumina Inc Nucleic acid synthesis techniques
CA3127071A1 (en) 2014-05-27 2015-12-03 Alex Aravanis Systems and methods for biochemical analysis including a base instrument and a removable cartridge
CN113584139A (en) 2014-06-03 2021-11-02 亿明达股份有限公司 Compositions, systems and methods for detecting events using tethers anchored to or near nanoparticles
EP3151733B1 (en) 2014-06-06 2020-04-15 The Regents Of The University Of Michigan Compositions and methods for characterizing and diagnosing periodontal disease
US20150353989A1 (en) 2014-06-09 2015-12-10 Illumina Cambridge Limited Sample preparation for nucleic acid amplification
WO2015189636A1 (en) 2014-06-13 2015-12-17 Illumina Cambridge Limited Methods and compositions for preparing sequencing libraries
EP3919621A1 (en) 2014-06-23 2021-12-08 The General Hospital Corporation Genomewide unbiased identification of dsbs evaluated by sequencing (guide-seq)
US11155809B2 (en) 2014-06-24 2021-10-26 Bio-Rad Laboratories, Inc. Digital PCR barcoding
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
PL3161154T3 (en) 2014-06-27 2020-10-19 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
CN107209188A (en) 2014-06-27 2017-09-26 雅培制药有限公司 Composition and method for detecting people Pegivirus 2 (HPgV 2)
JP6652512B2 (en) 2014-06-30 2020-02-26 イラミーナ インコーポレーテッド Methods and compositions using unilateral transfer
CA2955356C (en) 2014-07-15 2024-01-02 Illumina, Inc. Biochemically activated electronic device
EP3172321B2 (en) 2014-07-21 2023-01-04 Illumina, Inc. Polynucleotide enrichment using crispr-cas systems
CN106715718B (en) 2014-07-24 2021-07-06 雅培分子公司 Compositions and methods for detecting and analyzing mycobacterium tuberculosis
GB201414098D0 (en) 2014-08-08 2014-09-24 Illumina Cambridge Ltd Modified nucleotide linkers
EP3183577B1 (en) 2014-08-21 2020-08-19 Illumina Cambridge Limited Reversible surface functionalization
WO2016040602A1 (en) 2014-09-11 2016-03-17 Epicentre Technologies Corporation Reduced representation bisulfite sequencing using uracil n-glycosylase (ung) and endonuclease iv
WO2016040607A1 (en) 2014-09-12 2016-03-17 Illumina, Inc. Compositions, systems, and methods for detecting the presence of polymer subunits using chemiluminescence
EP3194627B1 (en) 2014-09-18 2023-08-16 Illumina, Inc. Methods and systems for analyzing nucleic acid sequencing data
WO2016054096A1 (en) 2014-09-30 2016-04-07 Illumina, Inc. Modified polymerases for improved incorporation of nucleotide analogues
WO2016153999A1 (en) 2015-03-25 2016-09-29 Life Technologies Corporation Modified nucleotides and uses thereof
JP6668336B2 (en) 2014-10-09 2020-03-18 イラミーナ インコーポレーテッド Method and apparatus for separating immiscible liquids and effectively isolating at least one liquid
EP3204519B1 (en) 2014-10-10 2020-04-29 Quantapore Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
US9897791B2 (en) 2014-10-16 2018-02-20 Illumina, Inc. Optical scanning systems for in situ genetic analysis
AU2015331739B2 (en) 2014-10-17 2021-12-02 Illumina Cambridge Limited Contiguity preserving transposition
CA2964790C (en) 2014-10-24 2023-02-21 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
ES2772127T3 (en) 2014-10-31 2020-07-07 Illumina Cambridge Ltd DNA copolymer polymers and coatings
GB201419731D0 (en) 2014-11-05 2014-12-17 Illumina Cambridge Ltd Sequencing from multiple primers to increase data rate and density
DK3215616T3 (en) 2014-11-05 2020-03-02 Illumina Cambridge Ltd Reducing DNA damage during sample preparation and sequencing using siderophore chelators
DK3218513T3 (en) 2014-11-11 2019-02-04 Illumina Inc Polynucleotide amplification using CRISPR-CAS systems
SG11201703693UA (en) 2014-11-11 2017-06-29 Illumina Cambridge Ltd Methods and arrays for producing and sequencing monoclonal clusters of nucleic acid
RU2582198C1 (en) * 2014-11-20 2016-04-20 Федеральное государственное бюджетное учреждение науки Лимнологический институт Сибирского отделения Российской академии наук (ЛИН СО РАН) Analogues of natural deoxyribonucleoside triphosphates and ribonucleoside triphosphates containing reporter fluorescent groups, for use in analytical bioorganic chemistry
CN104458686B (en) * 2014-12-02 2017-01-18 公安部第一研究所 DNA fluorescence spectrum collecting method based on characteristic molecular weight interior label quantitative analysis
US10350570B2 (en) 2014-12-15 2019-07-16 Illumina, Inc. Compositions and methods for single molecular placement on a substrate
EP3234115B1 (en) 2014-12-16 2020-07-22 Life Technologies Corporation Polymerase compositions and methods of making and using same
KR102593647B1 (en) 2014-12-18 2023-10-26 라이프 테크놀로지스 코포레이션 High data rate integrated circuit with transmitter configuration
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
EP3234575B1 (en) 2014-12-18 2023-01-25 Life Technologies Corporation Apparatus for measuring analytes using large scale fet arrays
PT3256604T (en) 2015-02-10 2020-05-18 Illumina Inc Methods and compositions for analyzing cellular components
US10208339B2 (en) 2015-02-19 2019-02-19 Takara Bio Usa, Inc. Systems and methods for whole genome amplification
US10697010B2 (en) 2015-02-19 2020-06-30 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
CN107407685B (en) 2015-02-20 2021-08-03 宝生物工程(美国)有限公司 Method for rapid and accurate dispensing, visualization and analysis of individual cells
CN107208158B (en) 2015-02-27 2022-01-28 贝克顿迪金森公司 Spatially addressable molecular barcode
CN107847930B (en) 2015-03-20 2020-06-30 亿明达股份有限公司 Fluid cartridges for use in a vertical or substantially vertical position
CA3225867A1 (en) 2015-03-24 2016-09-29 Illumina, Inc. Methods, carrier assemblies, and systems for imaging samples for biological or chemical analysis
EP3835431B1 (en) 2015-03-30 2022-11-02 Becton, Dickinson and Company Methods for combinatorial barcoding
EP3277839B1 (en) 2015-03-31 2020-12-02 Illumina Cambridge Limited Surface concatamerization of templates
EP3530752B1 (en) 2015-04-10 2021-03-24 Spatial Transcriptomics AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
CN112229834A (en) 2015-04-14 2021-01-15 亿明达股份有限公司 Structured substrate for improved detection of light emission and method relating thereto
WO2016168561A1 (en) 2015-04-15 2016-10-20 Sundaresan Tilak K Lna-based mutant enrichment next-generation sequencing assays
EP3286326A1 (en) 2015-04-23 2018-02-28 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
ES2826880T3 (en) 2015-05-11 2021-05-19 Illumina Inc Platform for the discovery and analysis of therapeutic agents
WO2016196358A1 (en) 2015-05-29 2016-12-08 Epicentre Technologies Corporation Methods of analyzing nucleic acids
AU2016271049B9 (en) 2015-05-29 2019-07-18 Illumina, Inc. Sample carrier and assay system for conducting designated reactions
CA2985545C (en) 2015-05-29 2021-02-09 Illumina Cambridge Limited Enhanced utilization of surface primers in clusters
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
IL293410B2 (en) 2015-06-03 2024-01-01 Illumina Inc Compositions, Systems, and Methods for Sequencing Polynucleotides using Tethers Anchored to Polymerases Adjacent to Nanopores
ES2877205T3 (en) 2015-07-06 2021-11-16 Illumina Cambridge Ltd Sample preparation for nucleic acid amplification
US10857537B2 (en) 2015-07-06 2020-12-08 Illumina, Inc. Balanced AC modulation for driving droplet operations electrodes
CN107924121B (en) 2015-07-07 2021-06-08 亿明达股份有限公司 Selective surface patterning via nanoimprinting
CA2992449A1 (en) 2015-07-14 2017-01-19 Abbott Molecular Inc. Purification of nucleic acids using copper-titanium oxides
US10526664B2 (en) 2015-07-14 2020-01-07 Abbott Molecular Inc. Compositions and methods for identifying drug resistant tuberculosis
CN107835857A (en) 2015-07-17 2018-03-23 亿明达股份有限公司 For the polymer sheet layer of application to be sequenced
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
WO2017014762A1 (en) * 2015-07-21 2017-01-26 Omniome, Inc. Nucleic acid sequencing methods and systems
ES2888626T3 (en) 2015-07-27 2022-01-05 Illumina Inc Spatial mapping of nucleic acid sequence information
JP6712606B2 (en) 2015-07-30 2020-06-24 イラミーナ インコーポレーテッド Orthogonal deblocking of nucleotides
ES2868195T3 (en) 2015-08-14 2021-10-21 Illumina Inc Systems and methods that use magnetically sensitive sensors to determine a genetic trait
CN116338219A (en) 2015-08-24 2023-06-27 亿明达股份有限公司 In-line accumulator and flow control system for biological and chemical assays
SG10201912283RA (en) 2015-08-28 2020-02-27 Illumina Inc Nucleic acid sequence analysis from single cells
US10906044B2 (en) 2015-09-02 2021-02-02 Illumina Cambridge Limited Methods of improving droplet operations in fluidic systems with a filler fluid including a surface regenerative silane
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
KR20180043369A (en) 2015-09-11 2018-04-27 더 제너럴 하스피탈 코포레이션 Complete call and sequencing of nuclease DSB (FIND-SEQ)
JP6940484B2 (en) 2015-09-11 2021-09-29 セルラー リサーチ, インコーポレイテッド Methods and compositions for library normalization
JP2018529353A (en) 2015-09-30 2018-10-11 ザ ジェネラル ホスピタル コーポレイション Comprehensive in vitro reporting of cleavage events by sequencing (CIRCLE-seq)
ES2925905T3 (en) 2015-10-01 2022-10-20 Life Technologies Corp Polymerase compositions and kits, and methods of use and manufacture thereof
CN108290157B (en) 2015-10-22 2021-08-17 亿明达股份有限公司 Fill fluid for fluid devices
EP3907295A1 (en) 2015-12-01 2021-11-10 Illumina, Inc. Method for compartmentalizing individual reactions in a line or an array of microwells
EP3798321A1 (en) 2015-12-17 2021-03-31 Illumina, Inc. Distinguishing methylation levels in complex biological samples
EP3400298B1 (en) 2016-01-08 2024-03-06 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
WO2017123533A1 (en) 2016-01-11 2017-07-20 Illumina, Inc. Detection apparatus having a microfluorometer, a fluidic system, and a flow cell latch clamp module
US10626390B2 (en) 2016-02-08 2020-04-21 RGENE, Inc. Multiple ligase compositions, systems, and methods
US20190062827A1 (en) 2016-03-14 2019-02-28 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
WO2017165703A1 (en) 2016-03-24 2017-09-28 Illumina, Inc. Photonic superlattice-based devices and compositions for use in luminescent imaging, and methods of using the same
WO2017172798A1 (en) 2016-03-28 2017-10-05 Illumina, Inc. Multi-plane microarrays
WO2017177017A1 (en) 2016-04-07 2017-10-12 Omniome, Inc. Methods of quantifying target nucleic acids and identifying sequence variants
WO2017176896A1 (en) 2016-04-07 2017-10-12 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
CN109072297B (en) * 2016-04-22 2022-09-13 加利福尼亚太平洋生物科学股份有限公司 Nucleic acid sequencing methods and systems using enhanced nucleotide-specific ternary complex formation detection
RU2731841C2 (en) 2016-04-22 2020-09-08 Иллюмина, Инк. Device and method based on photon structures for use in obtaining luminescent images of sites within pixel
CA3021769C (en) 2016-04-29 2021-11-23 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
AU2017261189B2 (en) 2016-05-02 2023-02-09 Becton, Dickinson And Company Accurate molecular barcoding
CN116397007A (en) 2016-05-11 2023-07-07 伊鲁米那股份有限公司 Polynucleotide enrichment and amplification Using the ARGONAUTE System
CN109313396B (en) 2016-05-18 2022-11-22 Illumina公司 Self-assembly patterning using patterned hydrophobic surfaces
KR102425463B1 (en) * 2016-05-20 2022-07-27 퀀텀-에스아이 인코포레이티드 Labeled Nucleotide Compositions and Methods for Nucleic Acid Sequencing
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3482196B1 (en) 2016-07-05 2022-02-23 Quantapore, Inc. Optically based nanopore sequencing
US11091795B2 (en) 2016-07-11 2021-08-17 Arizona Board Of Regents On Behalf Of The University Of Arizona Compositions and methods for diagnosing and treating arrhythmias
EP3875603A1 (en) 2016-07-12 2021-09-08 Life Technologies Corporation Compositions and methods for detecting nucleic acid regions
JP7075394B2 (en) 2016-07-21 2022-05-25 タカラ バイオ ユーエスエー, インコーポレイテッド Multi-Z imaging and dispensing using a multi-well device
JP7155021B2 (en) 2016-07-22 2022-10-18 オレゴン ヘルス アンド サイエンス ユニヴァーシティ A single-cell whole-genome library and a combinatorial indexing method for creating it
CN109844136A (en) 2016-08-15 2019-06-04 欧姆尼奥姆股份有限公司 The method and system of sequencing nucleic acid
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
US11543417B2 (en) 2016-08-29 2023-01-03 Oslo Universitetssykehus Hf ChIP-seq assays
CN109791157B (en) 2016-09-26 2022-06-07 贝克顿迪金森公司 Measuring protein expression using reagents with barcoded oligonucleotide sequences
WO2018064116A1 (en) 2016-09-28 2018-04-05 Illumina, Inc. Methods and systems for data compression
IL290157B2 (en) 2016-10-14 2023-09-01 Illumina Inc Cartridge assembly
WO2018075785A1 (en) 2016-10-19 2018-04-26 Illumina, Inc. Methods for chemical ligation of nucleic acids
JP7228510B2 (en) 2016-11-08 2023-02-24 ベクトン・ディキンソン・アンド・カンパニー Cell labeling method
CN109952612B (en) 2016-11-08 2023-12-01 贝克顿迪金森公司 Method for classifying expression profiles
CA3044231A1 (en) 2016-11-16 2018-05-24 Illumina, Inc. Validation methods and systems for sequence variant calls
GB201619458D0 (en) 2016-11-17 2017-01-04 Spatial Transcriptomics Ab Method for spatial tagging and analysing nucleic acids in a biological specimen
BR122021009064B1 (en) 2016-12-09 2022-04-12 The Broad Institute, Inc. System, method and device for detecting the presence of one or more polypeptides in a sample
WO2018118971A1 (en) 2016-12-19 2018-06-28 Bio-Rad Laboratories, Inc. Droplet tagging contiguity preserved tagmented dna
US20180195115A1 (en) 2016-12-22 2018-07-12 Illumina, Inc. Arrays with quality control tracers
RU2765075C2 (en) 2016-12-22 2022-01-25 Иллюмина, Инк. Biochip including sequencing primer and non-sequencing unit
RU2760391C2 (en) 2016-12-22 2021-11-24 Иллюмина, Инк. Biochips including resin film and structured polymer layer
AU2017385424A1 (en) * 2016-12-27 2019-08-01 Qingdao MGI Tech Co., Ltd. Single fluorescent dye based sequencing method
US11248254B2 (en) 2016-12-30 2022-02-15 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
GB201704754D0 (en) 2017-01-05 2017-05-10 Illumina Inc Kinetic exclusion amplification of nucleic acid libraries
US11150179B2 (en) 2017-01-06 2021-10-19 Illumina, Inc. Phasing correction
EP3568490B1 (en) 2017-01-10 2021-03-10 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent dna binding
EP3568234B1 (en) 2017-01-13 2023-09-06 Cellular Research, Inc. Hydrophilic coating of fluidic channels
KR102326612B1 (en) 2017-01-17 2021-11-15 일루미나, 인코포레이티드 Determination of oncogenic splice variants
WO2018136248A1 (en) 2017-01-18 2018-07-26 Illuminia, Inc. Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
EP3565900B1 (en) 2017-01-20 2021-04-07 Omniome, Inc. Genotyping by polymerase binding
SG11201906567YA (en) 2017-01-20 2019-08-27 Omniome Inc Allele-specific capture of nucleic acids
AU2018210944B2 (en) 2017-01-20 2021-07-22 Pacific Biosciences Of California, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
EP3354746B1 (en) 2017-01-30 2019-05-29 Gregor Mendel Institute of Molecular Plant Biology GmbH Novel spike-in oligonucleotides for normalization of sequence data
EP3577232A1 (en) 2017-02-01 2019-12-11 Cellular Research, Inc. Selective amplification using blocking oligonucleotides
GB201701686D0 (en) 2017-02-01 2017-03-15 Illunina Inc System & method with fiducials having offset layouts
GB201701688D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials in non-recliner layouts
GB201701689D0 (en) 2017-02-01 2017-03-15 Illumia Inc System and method with fiducials of non-closed shapes
WO2018152162A1 (en) 2017-02-15 2018-08-23 Omniome, Inc. Distinguishing sequences by detecting polymerase dissociation
MX2018015529A (en) 2017-02-21 2019-05-16 Illumina Inc Tagmentation using immobilized transposomes with linkers.
US11174515B2 (en) 2017-03-15 2021-11-16 The Broad Institute, Inc. CRISPR effector system based diagnostics
KR20190140918A (en) 2017-03-15 2019-12-20 더 브로드 인스티튜트, 인코퍼레이티드 CRISPR effector system-based diagnostics for virus detection
US11104937B2 (en) 2017-03-15 2021-08-31 The Broad Institute, Inc. CRISPR effector system based diagnostics
US11021740B2 (en) 2017-03-15 2021-06-01 The Broad Institute, Inc. Devices for CRISPR effector system based diagnostics
EP3601560A1 (en) 2017-03-20 2020-02-05 Illumina, Inc. Methods and compositions for preparing nucleic acid libraries
WO2018175399A1 (en) 2017-03-24 2018-09-27 Bio-Rad Laboratories, Inc. Universal hairpin primers
WO2018187013A1 (en) 2017-04-04 2018-10-11 Omniome, Inc. Fluidic apparatus and methods useful for chemical and biological reactions
EP3913053A1 (en) 2017-04-23 2021-11-24 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
ES2929837T3 (en) 2017-04-23 2022-12-02 Illumina Cambridge Ltd Compositions and methods to improve sample identification in indexed nucleic acid libraries
WO2018200386A1 (en) 2017-04-23 2018-11-01 Illumina, Inc. Compositions and methods for improving sample identification in indexed nucleic acid libraries
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US9951385B1 (en) 2017-04-25 2018-04-24 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
US11028435B2 (en) 2017-05-01 2021-06-08 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
AU2018266377A1 (en) 2017-05-08 2019-11-14 Illumina, Inc. Universal short adapters for indexing of polynucleotide samples
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
EP4293122A3 (en) 2017-06-07 2024-01-24 Oregon Health & Science University Single cell whole genome libraries for methylation sequencing
EP3634992B1 (en) 2017-06-08 2024-03-27 The Brigham And Women's Hospital, Inc. Methods and compositions for identifying epitopes
US11186862B2 (en) 2017-06-20 2021-11-30 Bio-Rad Laboratories, Inc. MDA using bead oligonucleotide
WO2018236631A1 (en) 2017-06-20 2018-12-27 Illumina, Inc. Methods and compositions for addressing inefficiencies in amplification reactions
JP7390285B2 (en) 2017-06-26 2023-12-01 ウニヴェルズィテート・フューア・ボーデンクルトゥーア・ウィーン Novel biomarker for detecting senescent cells
US11578180B2 (en) 2017-07-18 2023-02-14 Pacific Biosciences Of California, Inc. Method of chemically modifying plastic surfaces
AU2018308098A1 (en) 2017-07-24 2020-01-30 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2019027767A1 (en) 2017-07-31 2019-02-07 Illumina Inc. Sequencing system with multiplexed biological sample aggregation
JP6998404B2 (en) 2017-08-01 2022-02-04 深▲セン▼恒特基因有限公司 Method for enriching and determining the target nucleotide sequence
US11352668B2 (en) 2017-08-01 2022-06-07 Illumina, Inc. Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells
CA3067048C (en) 2017-08-01 2024-03-05 Illumina, Inc. Hydrogel beads for nucleotide sequencing
EP3668651B1 (en) 2017-08-15 2024-05-01 Pacific Biosciences of California, Inc. Methods using a scanning apparatus for sequencing nucleic acids
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
CA3075932A1 (en) 2017-09-20 2019-03-28 Guardant Health, Inc. Methods and systems for differentiating somatic and germline variants
NZ759818A (en) 2017-10-16 2022-04-29 Illumina Inc Semi-supervised learning for training an ensemble of deep convolutional neural networks
KR102317911B1 (en) 2017-10-16 2021-10-26 일루미나, 인코포레이티드 Deep learning-based splice site classification
WO2019079593A1 (en) 2017-10-19 2019-04-25 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
CN111295443B (en) 2017-11-02 2024-04-16 生物辐射实验室股份有限公司 Transposase-based genomic analysis
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
US11378544B2 (en) 2018-01-08 2022-07-05 Illumina, Inc. High-throughput sequencing with semiconductor-based detection
NZ759656A (en) 2018-01-08 2022-07-01 Illumina Inc Systems and devices for high-throughput sequencing with semiconductor-based detection
CN110832510A (en) 2018-01-15 2020-02-21 因美纳有限公司 Variant classifier based on deep learning
RU2020128413A (en) 2018-01-29 2022-04-01 Зе Броад Институт, Инк. DIAGNOSTICS BASED ON THE CRISPR EFFECTOR SYSTEM
EP3746552B1 (en) 2018-01-31 2023-12-27 Bio-Rad Laboratories, Inc. Methods and compositions for deconvoluting partition barcodes
BR112020015905A2 (en) 2018-02-06 2020-12-15 Omniome, Inc. COMPOSITIONS AND TECHNIQUES FOR NUCLEIC ACID INITIATOR EXTENSION
EP4083225A1 (en) 2018-02-13 2022-11-02 Illumina, Inc. Dna sequencing using hydrogel beads
US20190367909A1 (en) 2018-04-02 2019-12-05 Illumina, Inc. Compositions and methods for making controls for sequence-based genetic testing
US20190318806A1 (en) 2018-04-12 2019-10-17 Illumina, Inc. Variant Classifier Based on Deep Neural Networks
US11512002B2 (en) 2018-04-18 2022-11-29 University Of Virginia Patent Foundation Silica materials and methods of making thereof
WO2019203986A1 (en) 2018-04-19 2019-10-24 Omniome, Inc. Improving accuracy of base calls in nucleic acid sequencing methods
KR20200026250A (en) 2018-04-20 2020-03-10 일루미나, 인코포레이티드 Methods for Encapsulating Single Cells, Encapsulated Cells and Uses thereof
JP2021521820A (en) 2018-04-26 2021-08-30 オムニオム インコーポレイテッドOmniome, Inc. Methods and Compositions for Stabilizing Nucleic Acid-Nucleotide-Polymerase Complex
EP3788171B1 (en) 2018-05-03 2023-04-05 Becton, Dickinson and Company High throughput multiomics sample analysis
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
US20210239700A1 (en) 2018-05-04 2021-08-05 Abbott Laboratories Hbv diagnostic, prognostic, and therapeutic methods and products
JP7100069B2 (en) 2018-05-15 2022-07-12 イルミナ インコーポレイテッド Compositions and Methods for Chemical Cleavage and Deprotection of Surface-Binding Oligonucleotides
CN110785499A (en) 2018-05-25 2020-02-11 伊鲁米那股份有限公司 Circulating RNA identification specific for preeclampsia
EP3802874A1 (en) 2018-05-31 2021-04-14 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
JP2021526791A (en) 2018-06-04 2021-10-11 ガーダント ヘルス, インコーポレイテッド Methods and systems for determining the cellular origin of cell-free nucleic acids
DK3810774T3 (en) 2018-06-04 2023-12-11 Illumina Inc HIGH-THROUGH-PUT SINGLE CELL TRANSCRIPTOME LIBRARIES AND METHODS OF PREPARATION AND USE
US20200251183A1 (en) 2018-07-11 2020-08-06 Illumina, Inc. Deep Learning-Based Framework for Identifying Sequence Patterns that Cause Sequence-Specific Errors (SSEs)
CA3107983A1 (en) 2018-07-23 2020-01-30 Guardant Health, Inc. Methods and systems for adjusting tumor mutational burden by tumor fraction and coverage
AU2019312152A1 (en) 2018-07-24 2021-02-18 Pacific Biosciences Of California, Inc. Serial formation of ternary complex species
WO2020033601A1 (en) 2018-08-07 2020-02-13 The Broad Institute, Inc. Novel cas12b enzymes and systems
JP7431802B2 (en) 2018-08-15 2024-02-15 イルミナ インコーポレイテッド Compositions and methods for improving library enrichment
CN109161536B (en) * 2018-08-20 2022-04-08 天津科技大学 Enzyme preparation for preparing uridylic acid and method for preparing uridylic acid by enzyme catalysis
US11479816B2 (en) 2018-08-20 2022-10-25 Bio-Rad Laboratories, Inc. Nucleotide sequence generation by barcode bead-colocalization in partitions
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
AU2019331907A1 (en) 2018-08-30 2021-04-08 Guardant Health, Inc. Methods and systems for detecting contamination between samples
SG11202101400UA (en) 2018-08-31 2021-03-30 Guardant Health Inc Microsatellite instability detection in cell-free dna
WO2020096691A2 (en) 2018-09-04 2020-05-14 Guardant Health, Inc. Methods and systems for detecting allelic imbalance in cell-free nucleic acid samples
US11242512B2 (en) 2018-09-17 2022-02-08 Omniome, Inc. Engineered polymerases for improved sequencing
SG11202101798QA (en) 2018-09-20 2021-04-29 Tamirna Gmbh Micro-rna signatures for the prediction of liver dysfunction
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
WO2020072816A1 (en) 2018-10-03 2020-04-09 The Broad Institute, Inc. Crispr effector system based diagnostics for hemorrhagic fever detection
CN113705585A (en) 2018-10-15 2021-11-26 因美纳有限公司 Method and system based on neural network implementation
SG11202102703VA (en) 2018-10-26 2021-04-29 Illumina Inc Modulating polymer beads for dna processing
CN113227393A (en) 2018-10-31 2021-08-06 夸登特健康公司 Methods, compositions, and systems for calibrating epigenetic zoning assays
CA3103719A1 (en) 2018-10-31 2020-05-07 Illumina, Inc. Polymerases, compositions, and methods of use
JP2022506546A (en) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー Single-cell whole transcriptome analysis using random priming
NL2022043B1 (en) 2018-11-21 2020-06-03 Akershus Univ Hf Tagmentation-Associated Multiplex PCR Enrichment Sequencing
FI3887540T3 (en) 2018-11-30 2023-09-14 Illumina Inc Analysis of multiple analytes using a single assay
CN211311477U (en) 2018-12-04 2020-08-21 欧姆尼欧美公司 Sequencing system
CA3103739A1 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
SG11202012762WA (en) 2018-12-05 2021-01-28 Illumina Cambridge Ltd Methods and compositions for cluster generation by bridge amplification
EP3894587A1 (en) 2018-12-10 2021-10-20 10X Genomics, Inc. Resolving spatial arrays by proximity-based deconvolution
GB201820341D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for transposase-mediated spatial tagging and analysing genomic DNA in a biological specimen
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
GB201820300D0 (en) 2018-12-13 2019-01-30 10X Genomics Inc Method for spatial tagging and analysing genomic DNA in a biological specimen
CN112639126A (en) 2018-12-14 2021-04-09 伊卢米纳剑桥有限公司 Reducing phasing with unlabeled nucleotides during sequencing
AU2019411266A1 (en) 2018-12-17 2021-01-07 Illumina Cambridge Limited Compositions for use in polyunucleotide sequencing
SG11202012758WA (en) 2018-12-17 2021-01-28 Illumina Cambridge Ltd Primer oligonucleotide for sequencing
CN112739830A (en) 2018-12-18 2021-04-30 伊卢米纳剑桥有限公司 Methods and compositions for paired-end sequencing using single surface primers
WO2020132103A1 (en) 2018-12-19 2020-06-25 Illumina, Inc. Methods for improving polynucleotide cluster clonality priority
JP2022514010A (en) 2018-12-20 2022-02-09 ガーダント ヘルス, インコーポレイテッド Methods, compositions, and systems for improving the recovery of nucleic acid molecules
WO2020132350A2 (en) 2018-12-20 2020-06-25 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
US11293061B2 (en) 2018-12-26 2022-04-05 Illumina Cambridge Limited Sequencing methods using nucleotides with 3′ AOM blocking group
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
KR20210114918A (en) 2019-01-11 2021-09-24 일루미나 케임브리지 리미티드 complex surface-bound transposomal complex
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914603A1 (en) 2019-01-23 2021-12-01 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
ES2945227T3 (en) 2019-01-23 2023-06-29 Becton Dickinson Co Antibody Associated Oligonucleotides
AU2020216438A1 (en) 2019-01-31 2021-07-29 Guardant Health, Inc. Compositions and methods for isolating cell-free DNA
US11499189B2 (en) 2019-02-14 2022-11-15 Pacific Biosciences Of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
CN113613787B (en) 2019-02-20 2023-06-13 加利福尼亚太平洋生物科学股份有限公司 Scanning device and method for detecting chemical and biological analytes
WO2020176659A1 (en) 2019-02-27 2020-09-03 Guardant Health, Inc. Methods and systems for determining the cellular origin of cell-free dna
AU2020232618A1 (en) 2019-03-01 2021-04-08 Illumina, Inc. High-throughput single-nuclei and single-cell libraries and methods of making and of using
NL2023310B1 (en) 2019-03-21 2020-09-28 Illumina Inc Training data generation for artificial intelligence-based sequencing
NL2023314B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based quality scoring
WO2020191389A1 (en) 2019-03-21 2020-09-24 Illumina, Inc. Training data generation for artificial intelligence-based sequencing
NL2023312B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based base calling
NL2023316B1 (en) 2019-03-21 2020-09-28 Illumina Inc Artificial intelligence-based sequencing
US11676685B2 (en) 2019-03-21 2023-06-13 Illumina, Inc. Artificial intelligence-based quality scoring
NL2023311B9 (en) 2019-03-21 2021-03-12 Illumina Inc Artificial intelligence-based generation of sequencing metadata
US11210554B2 (en) 2019-03-21 2021-12-28 Illumina, Inc. Artificial intelligence-based generation of sequencing metadata
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US11593649B2 (en) 2019-05-16 2023-02-28 Illumina, Inc. Base calling using convolutions
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
US11939636B2 (en) 2019-05-31 2024-03-26 Guardant Health, Inc. Methods and systems for improving patient monitoring after surgery
US11644406B2 (en) 2019-06-11 2023-05-09 Pacific Biosciences Of California, Inc. Calibrated focus sensing
EP3997224A1 (en) 2019-07-12 2022-05-18 Illumina Cambridge Limited Nucleic acid library preparation using electrophoresis
US20220154173A1 (en) 2019-07-12 2022-05-19 Iiiumina Cambridge Limited Compositions and Methods for Preparing Nucleic Acid Sequencing Libraries Using CRISPR/CAS9 Immobilized on a Solid Support
US11377655B2 (en) 2019-07-16 2022-07-05 Pacific Biosciences Of California, Inc. Synthetic nucleic acids having non-natural structures
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
US10656368B1 (en) 2019-07-24 2020-05-19 Omniome, Inc. Method and system for biological imaging using a wide field objective lens
EP4028407B1 (en) 2019-09-10 2023-08-16 Pacific Biosciences of California, Inc. Reversible modification of nucleotides
WO2021050962A1 (en) 2019-09-11 2021-03-18 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Cancer detection and classification
WO2021076152A1 (en) 2019-10-18 2021-04-22 Omniome, Inc. Methods and compositions for capping nucleic acids
EP3812472B1 (en) 2019-10-21 2022-11-23 Albert-Ludwigs-Universität Freiburg A truly unbiased in vitro assay to profile off-target activity of one or more target-specific programmable nucleases in cells (abnoba-seq)
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
EP4055155A1 (en) 2019-11-08 2022-09-14 Pacific Biosciences of California, Inc. Engineered polymerases for improved sequencing by binding
US11773436B2 (en) 2019-11-08 2023-10-03 Becton, Dickinson And Company Using random priming to obtain full-length V(D)J information for immune repertoire sequencing
JP2023501760A (en) 2019-11-22 2023-01-19 イルミナ インコーポレイテッド A circulating RNA signature specific to pre-eclampsia
CA3157560A1 (en) 2019-11-26 2021-06-03 Dustin Howard HITE Methods, compositions and systems for improving the binding of methylated polynucleotides
DE202019106695U1 (en) 2019-12-02 2020-03-19 Omniome, Inc. System for sequencing nucleic acids in fluid foam
DE202019106694U1 (en) 2019-12-02 2020-03-19 Omniome, Inc. System for sequencing nucleic acids in fluid foam
WO2021113287A1 (en) 2019-12-04 2021-06-10 Illumina, Inc. Preparation of dna sequencing libraries for detection of dna pathogens in plasma
BR112021019640A2 (en) 2019-12-19 2022-06-21 Illumina Inc High-throughput single cell libraries and methods of preparation and use
EP3891300B1 (en) 2019-12-23 2023-03-29 10X Genomics, Inc. Methods for spatial analysis using rna-templated ligation
CN115244184A (en) 2020-01-13 2022-10-25 贝克顿迪金森公司 Methods and compositions for quantifying protein and RNA
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
WO2021152586A1 (en) 2020-01-30 2021-08-05 Yeda Research And Development Co. Ltd. Methods of analyzing microbiome, immunoglobulin profile and physiological state
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US20230054204A1 (en) 2020-02-04 2023-02-23 Pacific Biosciences Of California, Inc. Flow cells and methods for their manufacture and use
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
CN111311265B (en) * 2020-02-13 2023-07-25 布比(北京)网络技术有限公司 Blockchain private transaction proving method, blockchain private transaction proving device, computer equipment and storage medium
US20210265018A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Knowledge Distillation and Gradient Pruning-Based Compression of Artificial Intelligence-Based Base Caller
WO2021168353A2 (en) 2020-02-20 2021-08-26 Illumina, Inc. Artificial intelligence-based many-to-many base calling
US20210265015A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Hardware Execution and Acceleration of Artificial Intelligence-Based Base Caller
US20210265016A1 (en) 2020-02-20 2021-08-26 Illumina, Inc. Data Compression for Artificial Intelligence-Based Base Calling
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
CA3173798A1 (en) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
MX2022012184A (en) 2020-03-30 2022-10-27 Illumina Inc Methods and compositions for preparing nucleic acid libraries.
WO2021214766A1 (en) 2020-04-21 2021-10-28 Yeda Research And Development Co. Ltd. Methods of diagnosing viral infections and vaccines thereto
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
WO2021222828A1 (en) 2020-04-30 2021-11-04 Guardant Health, Inc. Methods for sequence determination using partitioned nucleic acids
US11188778B1 (en) 2020-05-05 2021-11-30 Illumina, Inc. Equalization-based image processing and spatial crosstalk attenuator
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
EP4146822A1 (en) 2020-05-05 2023-03-15 Pacific Biosciences of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
KR20230035237A (en) 2020-05-12 2023-03-13 일루미나, 인코포레이티드 Generation of Nucleic Acids with Modified Bases Using Recombinant Terminal Deoxynucleotidyl Transferases
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
WO2021231921A1 (en) 2020-05-14 2021-11-18 Guardant Health, Inc. Homologous recombination repair deficiency detection
WO2021236929A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
WO2021237087A1 (en) 2020-05-22 2021-11-25 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
WO2021247568A1 (en) 2020-06-02 2021-12-09 10X Genomics, Inc. Spatial trancriptomics for antigen-receptors
AU2021283174A1 (en) 2020-06-02 2023-01-05 10X Genomics, Inc. Nucleic acid library methods
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
WO2021252617A1 (en) 2020-06-09 2021-12-16 Illumina, Inc. Methods for increasing yield of sequencing libraries
WO2021252591A1 (en) 2020-06-10 2021-12-16 10X Genomics, Inc. Methods for determining a location of an analyte in a biological sample
WO2021252800A1 (en) 2020-06-11 2021-12-16 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
CN115867560A (en) 2020-06-22 2023-03-28 伊鲁米纳剑桥有限公司 Nucleosides and nucleotides having 3' acetal capping groups
CN116034166A (en) 2020-06-25 2023-04-28 10X基因组学有限公司 Spatial analysis of DNA methylation
IL299052A (en) 2020-07-02 2023-02-01 Illumina Inc A method to calibrate nucleic acid library seeding efficiency in flowcells
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
MX2023000345A (en) 2020-07-08 2023-02-13 Illumina Inc Beads as transposome carriers.
WO2023282916A1 (en) 2021-07-09 2023-01-12 Guardant Health, Inc. Methods of detecting genomic rearrangements using cell free nucleic acids
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US11946044B2 (en) 2020-07-30 2024-04-02 Guardant Health, Inc. Methods for isolating cell-free DNA
EP4192951A1 (en) 2020-08-06 2023-06-14 Illumina, Inc. Preparation of rna and dna sequencing libraries using bead-linked transposomes
JP2023537850A (en) 2020-08-18 2023-09-06 イルミナ インコーポレイテッド Sequence-specific targeted transposition and selection and selection of nucleic acids
EP4205126A1 (en) 2020-08-25 2023-07-05 Guardant Health, Inc. Methods and systems for predicting an origin of a variant
US20220067489A1 (en) 2020-08-28 2022-03-03 Illumina, Inc. Detecting and Filtering Clusters Based on Artificial Intelligence-Predicted Base Calls
JP2023540177A (en) 2020-09-11 2023-09-22 イルミナ ケンブリッジ リミテッド How to enrich target sequences from sequencing libraries using hairpin adapters
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
CA3193183A1 (en) 2020-09-30 2022-04-07 Andrew Kennedy Compositions and methods for analyzing dna using partitioning and a methylation-dependent nuclease
CN116438319A (en) 2020-10-21 2023-07-14 Illumina公司 Sequencing templates comprising multiple inserts, compositions and methods for improving sequencing throughput
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4267757A1 (en) 2020-12-23 2023-11-01 Guardant Health, Inc. Methods and systems for analyzing methylated polynucleotides
WO2022155331A1 (en) 2021-01-13 2022-07-21 Pacific Biosciences Of California, Inc. Surface structuring with colloidal assembly
WO2022165188A1 (en) 2021-01-29 2022-08-04 Illumina, Inc. Methods, compositions and kits to improve seeding efficiency of flow cells with polynucleotides
JP2024506304A (en) 2021-02-04 2024-02-13 イルミナ インコーポレイテッド Long-indexed concatenated read generation on transposome-bound beads
US20240043915A1 (en) 2021-02-13 2024-02-08 The General Hospital Corporation Methods and compositions for in situ macromolecule detection and uses thereof
WO2022187862A1 (en) 2021-03-05 2022-09-09 Guardant Health, Inc. Methods and related aspects for analyzing molecular response
US20220344004A1 (en) 2021-03-09 2022-10-27 Guardant Health, Inc. Detecting the presence of a tumor based on off-target polynucleotide sequencing data
WO2022197754A1 (en) 2021-03-16 2022-09-22 Illumina Software, Inc. Neural network parameter quantization for base calling
EP4301870A1 (en) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
CN117222747A (en) 2021-03-22 2023-12-12 伊鲁米纳剑桥有限公司 Methods for improving clonality of nucleic acid clusters
IL307177A (en) 2021-03-29 2023-11-01 Illumina Inc Compositions and methods for assessing dna damage in a library and normalizing amplicon size bias
CA3214278A1 (en) 2021-03-29 2022-10-06 Illumina, Inc Improved methods of library preparation
IL307172A (en) 2021-03-30 2023-11-01 Illumina Inc Improved methods of isothermal complementary dna and library preparation
AU2022248999A1 (en) 2021-03-31 2023-02-02 Illumina, Inc. Artificial intelligence-based base caller with contextual awareness
KR20230164668A (en) 2021-03-31 2023-12-04 일루미나, 인코포레이티드 Method for manufacturing directed tagged sequencing libraries using transposon-based technology using unique molecular identifiers for error correction
BR112023019465A2 (en) 2021-04-02 2023-12-05 Illumina Software Inc MACHINE LEARNING MODEL FOR DETECTING A BUBBLE WITHIN A NUCLEOTIDE SAMPLE SLIDE FOR SEQUENCING
US20220336054A1 (en) 2021-04-15 2022-10-20 Illumina, Inc. Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures
EP4337786A1 (en) 2021-05-10 2024-03-20 Pacific Biosciences of California, Inc. Single-molecule seeding and amplification on a surface
US20220356515A1 (en) 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
US20220396832A1 (en) 2021-05-20 2022-12-15 Illumina Cambridge Limited Compositions and methods for sequencing by synthesis
CN117677435A (en) 2021-06-15 2024-03-08 伊鲁米纳公司 Surface functionalization of non-aqueous gels for sequencing
EP4359555A1 (en) 2021-06-23 2024-05-01 Illumina, Inc. Compositions, methods, kits, cartridges, and systems for sequencing reagents
US20220415443A1 (en) 2021-06-29 2022-12-29 Illumina, Inc. Machine-learning model for generating confidence classifications for genomic coordinates
US20220415442A1 (en) 2021-06-29 2022-12-29 Illumina Software, Inc. Signal-to-noise-ratio metric for determining nucleotide-base calls and base-call quality
WO2023278184A1 (en) 2021-06-29 2023-01-05 Illumina, Inc. Methods and systems to correct crosstalk in illumination emitted from reaction sites
KR20240027599A (en) 2021-06-29 2024-03-04 일루미나, 인코포레이티드 Self-learning base caller trained using oligo sequences
US20230005253A1 (en) 2021-07-01 2023-01-05 Illumina, Inc. Efficient artificial intelligence-based base calling of index sequences
WO2023287617A1 (en) 2021-07-13 2023-01-19 Illumina, Inc. Methods and systems for real time extraction of crosstalk in illumination emitted from reaction sites
KR20240031968A (en) 2021-07-19 2024-03-08 일루미나, 인코포레이티드 Intensity extraction with interpolation and adaptation to base calling
US11455487B1 (en) 2021-10-26 2022-09-27 Illumina Software, Inc. Intensity extraction and crosstalk attenuation using interpolation and adaptation for base calling
US20230021577A1 (en) 2021-07-23 2023-01-26 Illumina Software, Inc. Machine-learning model for recalibrating nucleotide-base calls
CN117813391A (en) 2021-07-23 2024-04-02 因美纳有限公司 Method for preparing substrate surface for DNA sequencing
IL309786A (en) 2021-07-28 2024-02-01 Illumina Inc Quality score calibration of basecalling systems
KR20240035413A (en) 2021-08-03 2024-03-15 일루미나, 인코포레이티드 Base calling using the multi-base caller model
US20230047225A1 (en) 2021-08-14 2023-02-16 Illumina, Inc. Polymerases, compositions, and methods of use
WO2023023500A1 (en) 2021-08-17 2023-02-23 Illumina, Inc. Methods and compositions for identifying methylated cytosines
EP4196605A1 (en) 2021-09-01 2023-06-21 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
WO2023044229A1 (en) 2021-09-17 2023-03-23 Illumina, Inc. Automatically identifying failure sources in nucleotide sequencing from base-call-error patterns
US20230095961A1 (en) 2021-09-21 2023-03-30 Illumina, Inc. Graph reference genome and base-calling approach using imputed haplotypes
WO2023049212A2 (en) 2021-09-22 2023-03-30 Illumina, Inc. State-based base calling
WO2023056328A2 (en) 2021-09-30 2023-04-06 Illumina, Inc. Solid supports and methods for depleting and/or enriching library fragments prepared from biosamples
US20230096386A1 (en) 2021-09-30 2023-03-30 Illumina Cambridge Limited Polynucleotide sequencing
CA3232183A1 (en) 2021-10-11 2023-04-20 Nautilus Subsidiary, Inc. Highly multiplexable analysis of proteins and proteomes
WO2023069927A1 (en) 2021-10-20 2023-04-27 Illumina, Inc. Methods for capturing library dna for sequencing
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
CN117581303A (en) 2021-12-02 2024-02-20 因美纳有限公司 Generating cluster-specific signal corrections for determining nucleotide base detection
US20230215515A1 (en) 2021-12-23 2023-07-06 Illumina Software, Inc. Facilitating secure execution of external workflows for genomic sequencing diagnostics
WO2023122363A1 (en) 2021-12-23 2023-06-29 Illumina Software, Inc. Dynamic graphical status summaries for nucelotide sequencing
US20230207050A1 (en) 2021-12-28 2023-06-29 Illumina Software, Inc. Machine learning model for recalibrating nucleotide base calls corresponding to target variants
WO2023129764A1 (en) 2021-12-29 2023-07-06 Illumina Software, Inc. Automatically switching variant analysis model versions for genomic analysis applications
AU2023208743A1 (en) 2022-01-20 2024-01-04 Illumina, Inc. Methods of detecting methylcytosine and hydroxymethylcytosine by sequencing
AU2023225949A1 (en) 2022-02-25 2024-01-18 Illumina, Inc. Machine-learning models for detecting and adjusting values for nucleotide methylation levels
WO2023164660A1 (en) 2022-02-25 2023-08-31 Illumina, Inc. Calibration sequences for nucelotide sequencing
US20230343414A1 (en) 2022-03-25 2023-10-26 Illumina, Inc. Sequence-to-sequence base calling
US20230314324A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
CA3223722A1 (en) 2022-04-07 2023-10-12 Illumina, Inc. Altered cytidine deaminases and methods of use
US20230360732A1 (en) 2022-04-25 2023-11-09 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023212601A1 (en) 2022-04-26 2023-11-02 Illumina, Inc. Machine-learning models for selecting oligonucleotide probes for array technologies
WO2023209606A1 (en) 2022-04-29 2023-11-02 Illumina Cambridge Limited Methods and systems for encapsulating lyophilised microspheres
US20230360725A1 (en) 2022-05-09 2023-11-09 Guardant Health, Inc. Detecting degradation based on strand bias
WO2023220627A1 (en) 2022-05-10 2023-11-16 Illumina Software, Inc. Adaptive neural network for nucelotide sequencing
US20230392207A1 (en) 2022-06-03 2023-12-07 Illumina, Inc. Circulating rna biomarkers for preeclampsia
WO2023239917A1 (en) 2022-06-09 2023-12-14 Illumina, Inc. Dependence of base calling on flow cell tilt
WO2023240494A1 (en) * 2022-06-15 2023-12-21 深圳华大智造科技股份有限公司 Sequencing buffer and method for improving stability of dntp modified with reversible blocking group
WO2023250504A1 (en) 2022-06-24 2023-12-28 Illumina Software, Inc. Improving split-read alignment by intelligently identifying and scoring candidate split groups
US20230420075A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Accelerators for a genotype imputation model
WO2024006705A1 (en) 2022-06-27 2024-01-04 Illumina Software, Inc. Improved human leukocyte antigen (hla) genotyping
US20230420082A1 (en) 2022-06-27 2023-12-28 Illumina Software, Inc. Generating and implementing a structural variation graph genome
CN116024320A (en) * 2022-07-13 2023-04-28 上海翔琼生物技术有限公司 Fluorescent quantitative PCR method for detecting nucleic acid
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
WO2024026356A1 (en) 2022-07-26 2024-02-01 Illumina, Inc. Rapid single-cell multiomics processing using an executable file
WO2024039516A1 (en) 2022-08-19 2024-02-22 Illumina, Inc. Third dna base pair site-specific dna detection
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073516A1 (en) 2022-09-29 2024-04-04 Illumina, Inc. A target-variant-reference panel for imputing target variants
WO2024073047A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Cytidine deaminases and methods of use in mapping modified cytosine nucleotides
US20240120027A1 (en) 2022-09-30 2024-04-11 Illumina, Inc. Machine-learning model for refining structural variant calls
WO2024069581A1 (en) 2022-09-30 2024-04-04 Illumina Singapore Pte. Ltd. Helicase-cytidine deaminase complexes and methods of use
WO2024073043A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Methods of using cpg binding proteins in mapping modified cytosine nucleotides
WO2024068971A1 (en) 2022-09-30 2024-04-04 Illumina, Inc. Polymerases, compositions, and methods of use
US20240127905A1 (en) 2022-10-05 2024-04-18 Illumina, Inc. Integrating variant calls from multiple sequencing pipelines utilizing a machine learning architecture
WO2024077202A2 (en) 2022-10-06 2024-04-11 Illumina, Inc. Probes for improving environmental sample surveillance
WO2024077152A1 (en) 2022-10-06 2024-04-11 Illumina, Inc. Probes for depleting abundant small noncoding rna
WO2024077162A2 (en) 2022-10-06 2024-04-11 Illumina, Inc. Probes for improving coronavirus sample surveillance
WO2024081649A1 (en) 2022-10-11 2024-04-18 Illumina, Inc. Detecting and correcting methylation values from methylation sequencing assays

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031834A1 (en) * 1997-01-15 1998-07-23 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
WO2000017330A1 (en) * 1998-09-21 2000-03-30 Shangai Institute Of Biochemistry Chinese Academy Of Sciences Dna polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US6086737A (en) * 1984-03-29 2000-07-11 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes and suitable labels therefor
US5366603A (en) * 1984-03-29 1994-11-22 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting useing laser diodes
US6207421B1 (en) * 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5230781A (en) * 1984-03-29 1993-07-27 Li-Cor, Inc. Sequencing near infrared and infrared fluorescence labeled DNA for detecting using laser diodes
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US4997928A (en) * 1988-09-15 1991-03-05 E. I. Du Pont De Nemours And Company Fluorescent reagents for the preparation of 5'-tagged oligonucleotides
US5547839A (en) * 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) * 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
US5401847A (en) * 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5232075A (en) * 1991-06-25 1993-08-03 New Venture Gear, Inc. Viscous coupling apparatus with coined plates
US5405747A (en) * 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US6048690A (en) * 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
JP3227189B2 (en) * 1991-12-25 2001-11-12 キヤノン株式会社 Apparatus provided with flexible cable and ink jet recording apparatus provided with the apparatus
US5403708A (en) * 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US5503980A (en) * 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5547835A (en) * 1993-01-07 1996-08-20 Sequenom, Inc. DNA sequencing by mass spectrometry
US5677196A (en) * 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
WO1995006138A1 (en) * 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5512462A (en) 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6593148B1 (en) * 1994-03-01 2003-07-15 Li-Cor, Inc. Cyanine dye compounds and labeling methods
US5601982A (en) * 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US5961923A (en) * 1995-04-25 1999-10-05 Irori Matrices with memories and uses thereof
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5661028A (en) * 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US5972603A (en) * 1996-02-09 1999-10-26 President And Fellows Of Harvard College DNA polymerase with modified processivity
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
US5858671A (en) * 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
US6403311B1 (en) * 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
CA2281205A1 (en) * 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
EP2202312B1 (en) * 1997-03-12 2016-01-06 Applied Biosystems, LLC DNA polymerases having improved labelled nucleotide incorporation properties
PT1017848E (en) 1997-07-28 2003-02-28 Medical Biosystems Ltd ANALYSIS OF NUCLEIC ACID SEQUENCES
JP3813818B2 (en) * 1998-05-01 2006-08-23 アリゾナ ボード オブ リージェンツ Method for determining the nucleotide sequence of oligonucleotides and DNA molecules
CA2339121A1 (en) 1998-07-30 2000-02-10 Shankar Balasubramanian Arrayed biomolecules and their use in sequencing
US6210896B1 (en) * 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) * 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6280939B1 (en) * 1998-09-01 2001-08-28 Veeco Instruments, Inc. Method and apparatus for DNA sequencing using a local sensitive force detector
DE19844931C1 (en) * 1998-09-30 2000-06-15 Stefan Seeger Procedures for DNA or RNA sequencing
US6221592B1 (en) * 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6044744A (en) * 1998-10-29 2000-04-04 At&T Corp. Fiber optic cable sheath removal tool
CA2355816C (en) * 1998-12-14 2007-10-30 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
EP1156420B1 (en) 1998-12-15 2005-07-06 Matsushita Electric Industrial Co., Ltd. Clock phase adjustment method, and integrated circuit and design method therefor
US6558945B1 (en) * 1999-03-08 2003-05-06 Aclara Biosciences, Inc. Method and device for rapid color detection
AU3174600A (en) * 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
US7056661B2 (en) * 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
AU7086800A (en) * 1999-08-30 2001-03-26 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The High speed parallel molecular nucleic acid sequencing
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
US6399335B1 (en) * 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6917726B2 (en) * 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US6936702B2 (en) * 2000-06-07 2005-08-30 Li-Cor, Inc. Charge-switch nucleotides
US6869764B2 (en) * 2000-06-07 2005-03-22 L--Cor, Inc. Nucleic acid sequencing using charge-switch nucleotides
DE60131194T2 (en) * 2000-07-07 2008-08-07 Visigen Biotechnologies, Inc., Bellaire SEQUENCE PROVISION IN REAL TIME
US20070172866A1 (en) * 2000-07-07 2007-07-26 Susan Hardin Methods for sequence determination using depolymerizing agent
DE60116510T2 (en) * 2000-09-19 2006-07-13 Li-Cor, Inc., Lincoln cyanine
WO2002044425A2 (en) * 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2002087123A1 (en) * 2001-04-25 2002-10-31 Tekelec Methods and systems for load sharing signaling messages among signaling links
US20040161741A1 (en) * 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US20030064400A1 (en) * 2001-08-24 2003-04-03 Li-Cor, Inc. Microfluidics system for single molecule DNA sequencing
ATE458067T1 (en) * 2001-08-29 2010-03-15 Ge Healthcare Bio Sciences LABELED NUCLEOSIDE POLYPHOSPHATES
US7052839B2 (en) * 2001-08-29 2006-05-30 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides and methods of use
US7223541B2 (en) * 2001-08-29 2007-05-29 Ge Healthcare Bio-Sciences Corp. Terminal-phosphate-labeled nucleotides and methods of use
US7033762B2 (en) * 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7005518B2 (en) * 2002-10-25 2006-02-28 Li-Cor, Inc. Phthalocyanine dyes
WO2004072238A2 (en) * 2003-02-05 2004-08-26 Amersham Biosciences Corp Terminal-phosphate-labeled nucleotides with new linkers
ES2355555T3 (en) * 2003-02-05 2011-03-28 Ge Healthcare Bio-Sciences Corp. AMPLIFICATION OF NUCLEIC ACIDS.
US7745116B2 (en) * 2003-04-08 2010-06-29 Pacific Biosciences Of California, Inc. Composition and method for nucleic acid sequencing
KR100558528B1 (en) * 2003-09-25 2006-03-10 동부아남반도체 주식회사 CMOS Image sensor and its fabricating method
GB2423819B (en) * 2004-09-17 2008-02-06 Pacific Biosciences California Apparatus and method for analysis of molecules
US7170050B2 (en) * 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US20070048748A1 (en) * 2004-09-24 2007-03-01 Li-Cor, Inc. Mutant polymerases for sequencing and genotyping
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
CA2596495A1 (en) * 2005-02-09 2006-08-17 Pacific Biosciences Of California, Inc. Nucleotide compositions and uses thereof
US7130041B2 (en) * 2005-03-02 2006-10-31 Li-Cor, Inc. On-chip spectral filtering using CCD array for imaging and spectroscopy
US8227621B2 (en) * 2005-06-30 2012-07-24 Li-Cor, Inc. Cyanine dyes and methods of use
US7509836B2 (en) * 2005-09-01 2009-03-31 Li-Cor, Inc. Gas flux system chamber design and positioning method
US7935310B2 (en) * 2005-11-28 2011-05-03 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrate and methods for making and using same
JP2009519717A (en) * 2005-12-16 2009-05-21 アプレラ コーポレイション Method and system for fixed phase sequencing
US20080076189A1 (en) * 2006-03-30 2008-03-27 Visigen Biotechnologies, Inc. Modified surfaces for the detection of biomolecules at the single molecule level
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998031834A1 (en) * 1997-01-15 1998-07-23 Incyte Pharmaceuticals, Inc. Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
WO2000017330A1 (en) * 1998-09-21 2000-03-30 Shangai Institute Of Biochemistry Chinese Academy Of Sciences Dna polymerase having ability to reduce innate selective discrimination against fluorescent dye-labeled dideoxynucleotides

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
FUREY W SCOTT ET AL: "Use of fluorescence resonance energy transfer to investigate the conformation of DNA substrates bound to the Klenow fragment", BIOCHEMISTRY, AMERICAN CHEMICAL SOCIETY. EASTON, PA, US, vol. 37, no. 9, 3 March 1998 (1998-03-03), pages 2979 - 2990, XP002171248, ISSN: 0006-2960 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9243284B2 (en) 2000-12-01 2016-01-26 Life Technologies Corporation Enzymatic nucleic acid synthesis: compositions and methods for inhibiting pyrophosphorolysis
US9588051B2 (en) 2004-09-17 2017-03-07 Pacific Biosciences Of California, Inc. Apparatus and method for performing nucleic acid analysis
US9365838B2 (en) 2009-03-27 2016-06-14 Life Technologies Corporation Conjugates of biomolecules to nanoparticles
US9365839B2 (en) 2009-03-27 2016-06-14 Life Technologies Corporation Polymerase compositions and methods
US9567629B2 (en) 2009-03-27 2017-02-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems

Also Published As

Publication number Publication date
US20070172862A1 (en) 2007-07-26
EP1975251A3 (en) 2009-03-25
JP2004513619A (en) 2004-05-13
US20100304367A1 (en) 2010-12-02
US20070275395A1 (en) 2007-11-29
EP1975251A2 (en) 2008-10-01
DE60131194D1 (en) 2007-12-13
US7329492B2 (en) 2008-02-12
US20070172868A1 (en) 2007-07-26
EP2100971A3 (en) 2009-11-25
US20070172858A1 (en) 2007-07-26
US20110014604A1 (en) 2011-01-20
US20070292867A1 (en) 2007-12-20
CA2415897A1 (en) 2002-01-17
WO2002004680A2 (en) 2002-01-17
US20050266424A1 (en) 2005-12-01
US20110059436A1 (en) 2011-03-10
US20070172863A1 (en) 2007-07-26
US20070172864A1 (en) 2007-07-26
US20100255463A1 (en) 2010-10-07
US20100317005A1 (en) 2010-12-16
EP1368460B1 (en) 2007-10-31
US20070184475A1 (en) 2007-08-09
US20110021383A1 (en) 2011-01-27
US20070172861A1 (en) 2007-07-26
CN100462433C (en) 2009-02-18
EP2100971A2 (en) 2009-09-16
US20070172865A1 (en) 2007-07-26
US20090305278A1 (en) 2009-12-10
EP1368460A2 (en) 2003-12-10
US20090275036A1 (en) 2009-11-05
ATE377093T1 (en) 2007-11-15
CN1553953A (en) 2004-12-08
US20070172867A1 (en) 2007-07-26
US20050260614A1 (en) 2005-11-24
JP2009240318A (en) 2009-10-22
AU8288101A (en) 2002-01-21
US20030064366A1 (en) 2003-04-03
JP2009118847A (en) 2009-06-04
DE60131194T2 (en) 2008-08-07
CN101525660A (en) 2009-09-09
AU2001282881B2 (en) 2007-06-14

Similar Documents

Publication Publication Date Title
WO2002004680A3 (en) Real-time sequence determination
DE69737450D1 (en) SEQUENCING BY LIGATION-CODED ADAPTER
EP1484416A3 (en) Method of identifying and quantifying specific fungi and bacteria
Dallas et al. Sex identification of the Eurasian otter Lutra lutra by PCR typing of spraints
WO2004013357A3 (en) Nucleotide sequences specific to francisella tularensis and methods for the detection of francisella tularensis
WO2005059103A3 (en) Corn plant mon88017 and compositions and methods for detection thereof
WO2002044425A3 (en) Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2002068579A3 (en) Kits, such as nucleic acid arrays, comprising a majority of human exons or transcripts, for detecting expression and other uses thereof
WO2001079563A3 (en) Degradable nucleic acid probes and nucleic acid detection methods
WO2000066607A8 (en) Detection using degradation of a tagged sequence
MXPA05005945A (en) Bentgrass event asr-368 and compositions and methods for detection thereof.
WO2005005667A3 (en) 2'-terminator nucleotide-related methods and systems
DE10010281B4 (en) Ligase / polymerase method for the detection of cytosine methylation in DNA samples
DE60134146D1 (en) DETECTION AND AMPLIFICATION OF RNA UNDER USING TARGETED DNA LIGATION BY RNA LIGASE
WO2000066777A3 (en) Assay based on the analysis of dna melting curves
FI956052A (en) Detection of Salmonella in a polymerase chain reactor
WO2005012548A3 (en) Self-hybridizing multiple target nucleic acid probes and methods of use
DE69332666T2 (en) METHOD FOR SIMPLE NUCLEOTIDE PRIMER EXTENSION FOR DETECTING SPECIFIC ALLELS AND KIT SUITABLE FOR THIS
WO2002061143A3 (en) Comparative analysis of nucleic acids using population tagging
WO2000022171A3 (en) Systems and methods for sequencing by hybridization
EP1689886A4 (en) Molecular marker associated with cmv resistance and use thereof
PT1362123E (en) Security system
ATE331813T1 (en) METHOD FOR DETECTING HOMO- OR HETEROZYGOTY OF AN ORGANISM USING MARKED PRIMERS AND RFLP
ATE408710T1 (en) DETECTION OF LEGIONELLA BY A METHOD USING PCR AND FRET
WO2003072721A3 (en) Detection by sliding template amplification

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH GM HR HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWE Wipo information: entry into national phase

Ref document number: 2001282881

Country of ref document: AU

Ref document number: 2415897

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001961632

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 018152384

Country of ref document: CN

WWP Wipo information: published in national office

Ref document number: 2001961632

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 2001282881

Country of ref document: AU

WWG Wipo information: grant in national office

Ref document number: 2001961632

Country of ref document: EP