WO2002014476A3 - Identifying and quantifying rnas and dnas associated with rna and dna binding proteins - Google Patents

Identifying and quantifying rnas and dnas associated with rna and dna binding proteins Download PDF

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Publication number
WO2002014476A3
WO2002014476A3 PCT/US2001/025804 US0125804W WO0214476A3 WO 2002014476 A3 WO2002014476 A3 WO 2002014476A3 US 0125804 W US0125804 W US 0125804W WO 0214476 A3 WO0214476 A3 WO 0214476A3
Authority
WO
WIPO (PCT)
Prior art keywords
rna
rnas
identifying
dna binding
quantifying
Prior art date
Application number
PCT/US2001/025804
Other languages
French (fr)
Other versions
WO2002014476A2 (en
Inventor
James H Eberwine
Original Assignee
Univ Pennsylvania
James H Eberwine
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pennsylvania, James H Eberwine filed Critical Univ Pennsylvania
Priority to US10/344,467 priority Critical patent/US7122313B2/en
Priority to AU2001288289A priority patent/AU2001288289A1/en
Publication of WO2002014476A2 publication Critical patent/WO2002014476A2/en
Publication of WO2002014476A3 publication Critical patent/WO2002014476A3/en
Priority to US11/518,719 priority patent/US20070003973A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Abstract

Methods and kits for identifying RNAs and DNAs associated with an RNA or DNA binding protein via a detector specific for the binding protein or protein bound thereto which is linked to an oligonucleotide capable of directing the synthesis of cDNA copies of RNA or DNA that are in close proximity to or in the vicinity of the bound detector are provided.
PCT/US2001/025804 2000-08-17 2001-08-17 Identifying and quantifying rnas and dnas associated with rna and dna binding proteins WO2002014476A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/344,467 US7122313B2 (en) 2001-08-17 2001-08-17 Methods and kits for identifying and quantifying RNAs and DNAs associated with RNA and DNA binding proteins
AU2001288289A AU2001288289A1 (en) 2000-08-17 2001-08-17 Methods and kits for identifying and quantifying rnas and dnas associated with rna and dna binding proteins
US11/518,719 US20070003973A1 (en) 2000-08-17 2006-09-11 Methods and kits for identifying and quantifying RNAs and DNAs associated with RNA and DNA binding proteins

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22585800P 2000-08-17 2000-08-17
US60/225,858 2000-08-17

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/518,719 Continuation US20070003973A1 (en) 2000-08-17 2006-09-11 Methods and kits for identifying and quantifying RNAs and DNAs associated with RNA and DNA binding proteins

Publications (2)

Publication Number Publication Date
WO2002014476A2 WO2002014476A2 (en) 2002-02-21
WO2002014476A3 true WO2002014476A3 (en) 2002-05-30

Family

ID=22846535

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/025804 WO2002014476A2 (en) 2000-08-17 2001-08-17 Identifying and quantifying rnas and dnas associated with rna and dna binding proteins

Country Status (2)

Country Link
AU (1) AU2001288289A1 (en)
WO (1) WO2002014476A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
WO2004048596A2 (en) 2002-11-21 2004-06-10 Epicentre Technologies Methods for using primers that encode one strand of a double-stranded promoter

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5922617A (en) * 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6087112A (en) * 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027890A (en) * 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US5922617A (en) * 1997-11-12 1999-07-13 Functional Genetics, Inc. Rapid screening assay methods and devices
US6087112A (en) * 1998-12-30 2000-07-11 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions

Also Published As

Publication number Publication date
AU2001288289A1 (en) 2002-02-25
WO2002014476A2 (en) 2002-02-21

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