WO2002016606A3 - Procede de mutagenese dirigee massive - Google Patents

Procede de mutagenese dirigee massive Download PDF

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Publication number
WO2002016606A3
WO2002016606A3 PCT/FR2001/002666 FR0102666W WO0216606A3 WO 2002016606 A3 WO2002016606 A3 WO 2002016606A3 FR 0102666 W FR0102666 W FR 0102666W WO 0216606 A3 WO0216606 A3 WO 0216606A3
Authority
WO
WIPO (PCT)
Prior art keywords
massive
directed mutagenesis
mutagenesis
directed
concerns
Prior art date
Application number
PCT/FR2001/002666
Other languages
English (en)
Other versions
WO2002016606A2 (fr
Inventor
Marc Delcourt
Stephane Blesa
Original Assignee
Biomethodes
Marc Delcourt
Stephane Blesa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomethodes, Marc Delcourt, Stephane Blesa filed Critical Biomethodes
Priority to US10/362,576 priority Critical patent/US7202086B2/en
Priority to DK01965350T priority patent/DK1311670T3/da
Priority to EP01965350A priority patent/EP1311670B1/fr
Priority to AU2001286009A priority patent/AU2001286009A1/en
Priority to CA2420721A priority patent/CA2420721C/fr
Priority to JP2002522277A priority patent/JP5068917B2/ja
Priority to DE60126786T priority patent/DE60126786T2/de
Publication of WO2002016606A2 publication Critical patent/WO2002016606A2/fr
Publication of WO2002016606A3 publication Critical patent/WO2002016606A3/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms

Abstract

La présente invention concerne le domaine de la biologie moléculaire et plus particulièrement celui de la mutagenèse. Elle a pour objet un procédé de mutagenèse dirigée à haut débit, c'est-à-dire la constitution de nombreux mutants dirigés en un temps et un nombre d'étapes réduits. Ce procédé sera donc qualifié mutagenèse massive.
PCT/FR2001/002666 2000-08-25 2001-08-24 Procede de mutagenese dirigee massive WO2002016606A2 (fr)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US10/362,576 US7202086B2 (en) 2000-08-25 2001-08-24 Method for massive directed mutagenesis
DK01965350T DK1311670T3 (da) 2000-08-25 2001-08-24 Fremgangsmåde til massiv dirigeret mutagenese
EP01965350A EP1311670B1 (fr) 2000-08-25 2001-08-24 Procede de mutagenese dirigee massive
AU2001286009A AU2001286009A1 (en) 2000-08-25 2001-08-24 Method for massive directed mutagenesis
CA2420721A CA2420721C (fr) 2000-08-25 2001-08-24 Procede de mutagenese dirigee massive
JP2002522277A JP5068917B2 (ja) 2000-08-25 2001-08-24 大量特異的変異導入方法
DE60126786T DE60126786T2 (de) 2000-08-25 2001-08-24 Verfahren zur ortspezifischen massenmutagenese

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR00/10962 2000-08-25
FR0010962A FR2813314B1 (fr) 2000-08-25 2000-08-25 Procede de mutagenese dirigee massive

Publications (2)

Publication Number Publication Date
WO2002016606A2 WO2002016606A2 (fr) 2002-02-28
WO2002016606A3 true WO2002016606A3 (fr) 2002-06-27

Family

ID=8853747

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2001/002666 WO2002016606A2 (fr) 2000-08-25 2001-08-24 Procede de mutagenese dirigee massive

Country Status (11)

Country Link
US (1) US7202086B2 (fr)
EP (1) EP1311670B1 (fr)
JP (1) JP5068917B2 (fr)
AT (1) ATE354653T1 (fr)
AU (1) AU2001286009A1 (fr)
CA (1) CA2420721C (fr)
DE (1) DE60126786T2 (fr)
DK (1) DK1311670T3 (fr)
ES (1) ES2282285T3 (fr)
FR (1) FR2813314B1 (fr)
WO (1) WO2002016606A2 (fr)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6958213B2 (en) 2000-12-12 2005-10-25 Alligator Bioscience Ab Method for in vitro molecular evolution of protein function
US20020086292A1 (en) 2000-12-22 2002-07-04 Shigeaki Harayama Synthesis of hybrid polynucleotide molecules using single-stranded polynucleotide molecules
US20050153283A1 (en) * 2002-08-08 2005-07-14 Padgett Hal S. Method of increasing complementarity in a heteroduplex
CA2436214C (fr) * 2001-02-02 2012-12-04 Large Scale Biology Corporation Procede destine a ameliorer la complementarite d'un heteroduplex
US20040142433A1 (en) * 2001-02-02 2004-07-22 Padgett Hal S. Polynucleotide sequence variants
US7838219B2 (en) * 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
FR2823219B1 (fr) * 2001-04-10 2003-07-04 Pasteur Institut Mutants de la desoxycytidine kinase possedant une activite enzymatique elargie
US7647184B2 (en) 2001-08-27 2010-01-12 Hanall Pharmaceuticals, Co. Ltd High throughput directed evolution by rational mutagenesis
US7078211B2 (en) * 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
ATE466085T1 (de) 2002-09-09 2010-05-15 Hanall Pharmaceutical Co Ltd Protease-resistente modifizierte interferon alpha polypeptide
ATE428779T1 (de) * 2003-12-18 2009-05-15 Biomethodes Verfahren zur ortspezifischen massenmutagenese
FR2883885B1 (fr) * 2005-04-05 2010-12-10 Biomethodes Recombinaison in vitro circulaire
FR2886943B1 (fr) 2005-06-10 2007-09-07 Biomethodes Sa Methode de selection de proteines stables dans des conditions physico-chimiques non standard
GB2432366B (en) * 2005-11-19 2007-11-21 Alligator Bioscience Ab A method for in vitro molecular evolution of protein function
FR2897068B1 (fr) * 2006-02-03 2008-04-18 Biomethodes Sa Kanamycine kinase ii amelioree et utilisations de celle-ci
JP2009529027A (ja) * 2006-03-08 2009-08-13 ビオメトード ヒトインターフェロンガンマ（ＩＦＮγ）変異体
CA2693102C (fr) * 2007-07-31 2020-03-10 Verenium Corporation Assemblage combinatoire multi-sites sur mesure
EP3514175A1 (fr) 2012-12-17 2019-07-24 Laboratoire Français du Fractionnement et des Biotechnologies Utilisation d'anticorps monoclonaux pour le traitement de l'inflammation et d'infections bacteriennes
BR112016001704A2 (pt) 2013-07-26 2017-09-19 Biomethodes Variante de endoglucanase i, ácido nucleico, vetor ou cassete de expressão, célula hospedeira, método de produção de uma variante de endoglucanase i, método de conversão de biomassa celulósica a açúcares monoméricos, e, método de produção de um produto de fermentação a partir da biomassa celulósica
WO2019126211A1 (fr) * 2017-12-20 2019-06-27 Basf Se Procédé de mutagénèse de saturation de site génique (gssm)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5798208A (en) * 1990-04-05 1998-08-25 Roberto Crea Walk-through mutagenesis

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Publication number Priority date Publication date Assignee Title
US4960707A (en) * 1987-08-17 1990-10-02 Associated Universities, Inc. Recombinant plasmids for encoding restriction enzymes DpnI and DpnII of streptococcus pneumontae
WO1993020096A1 (fr) * 1991-05-08 1993-10-14 Stratagene Bibliotheques d'oligonucleotides utiles pour la production d'amorces
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5830696A (en) * 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
DE69934088T2 (de) * 1998-01-09 2007-06-21 University Of Utah Research Foundation, Salt Lake City Verfahren zur in vitro amplifikation zirkulärer dna
US6368861B1 (en) * 1999-01-19 2002-04-09 Maxygen, Inc. Oligonucleotide mediated nucleic acid recombination

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5798208A (en) * 1990-04-05 1998-08-25 Roberto Crea Walk-through mutagenesis

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
XIAO, G. ET AL.: "Construction and screening of a multi-point site-specific mutant library of subtilisin E with a set of oligonucleotides", SCIENCE IN CHINA, SERIES C: LIFE SCIENCES, vol. 40, no. 4, 1997, pages 337 - 344, XP000993443 *

Also Published As

Publication number Publication date
FR2813314A1 (fr) 2002-03-01
ATE354653T1 (de) 2007-03-15
AU2001286009A1 (en) 2002-03-04
JP2004507243A (ja) 2004-03-11
DK1311670T3 (da) 2007-06-18
FR2813314B1 (fr) 2004-05-07
EP1311670B1 (fr) 2007-02-21
CA2420721A1 (fr) 2002-02-28
US20040048268A1 (en) 2004-03-11
DE60126786D1 (de) 2007-04-05
DE60126786T2 (de) 2007-11-08
JP5068917B2 (ja) 2012-11-07
US7202086B2 (en) 2007-04-10
WO2002016606A2 (fr) 2002-02-28
CA2420721C (fr) 2013-11-12
EP1311670A2 (fr) 2003-05-21
ES2282285T3 (es) 2007-10-16

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