WO2002020717A3 - Methods for simultaneous control of lignin content and composition, and cellulose content in plants - Google Patents

Methods for simultaneous control of lignin content and composition, and cellulose content in plants Download PDF

Info

Publication number
WO2002020717A3
WO2002020717A3 PCT/US2001/027445 US0127445W WO0220717A3 WO 2002020717 A3 WO2002020717 A3 WO 2002020717A3 US 0127445 W US0127445 W US 0127445W WO 0220717 A3 WO0220717 A3 WO 0220717A3
Authority
WO
WIPO (PCT)
Prior art keywords
plants
content
composition
methods
genes
Prior art date
Application number
PCT/US2001/027445
Other languages
French (fr)
Other versions
WO2002020717A2 (en
Inventor
Vincent Lee C Chiang
Laigeng Li
Original Assignee
Univ Michigan Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Michigan Tech filed Critical Univ Michigan Tech
Priority to AU2001288716A priority Critical patent/AU2001288716A1/en
Priority to BR0113699-2A priority patent/BR0113699A/en
Priority to JP2002525724A priority patent/JP4871487B2/en
Priority to IL15476001A priority patent/IL154760A0/en
Publication of WO2002020717A2 publication Critical patent/WO2002020717A2/en
Priority to IL154760A priority patent/IL154760A/en
Publication of WO2002020717A3 publication Critical patent/WO2002020717A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8255Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving lignin biosynthesis

Abstract

The present invention relates to a method of concurrently introducing multiple genes into plants and trees is provided. The method includes simultaneous transformation of plants with multiple genes from the phenylpropanoid pathways including 4CL, CAld5H, AldOMT, SAD and CAD genes and combinations thereof to produce various lines of transgenic plants displaying altered agronomic traits. The agronomic traits of the plants are regulated by the orientation of the specific genes and the selected gene combinations, which are incorporated into the plant genome.
PCT/US2001/027445 2000-09-05 2001-09-05 Methods for simultaneous control of lignin content and composition, and cellulose content in plants WO2002020717A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU2001288716A AU2001288716A1 (en) 2000-09-05 2001-09-05 Methods for simultaneous control of lignin content and composition, and cellulose content in plants
BR0113699-2A BR0113699A (en) 2000-09-05 2001-09-05 Methods for simultaneous control of lignin content and composition, and cellulose content in plants
JP2002525724A JP4871487B2 (en) 2000-09-05 2001-09-05 Method for simultaneous control of lignin content and composition and cellulose content in plants
IL15476001A IL154760A0 (en) 2000-09-05 2001-09-05 Methods for simultaneous control of lignin content and compositions, and cellulose content in plants
IL154760A IL154760A (en) 2000-09-05 2003-03-05 Methods for the preparation of transformed plants in which the lignin content and composition and cellulose content in plants is altered

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23008600P 2000-09-05 2000-09-05
US60/230,086 2000-09-05

Publications (2)

Publication Number Publication Date
WO2002020717A2 WO2002020717A2 (en) 2002-03-14
WO2002020717A3 true WO2002020717A3 (en) 2009-06-11

Family

ID=22863901

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2001/027534 WO2002020812A1 (en) 2000-09-05 2001-09-05 Genetic engineering of syringyl-enriched lignin in plants
PCT/US2001/027445 WO2002020717A2 (en) 2000-09-05 2001-09-05 Methods for simultaneous control of lignin content and composition, and cellulose content in plants

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2001/027534 WO2002020812A1 (en) 2000-09-05 2001-09-05 Genetic engineering of syringyl-enriched lignin in plants

Country Status (11)

Country Link
US (4) US6855864B2 (en)
EP (1) EP1320617A4 (en)
JP (2) JP2004520015A (en)
CN (1) CN1473198A (en)
AR (2) AR030616A1 (en)
AU (3) AU2001288765B2 (en)
BR (2) BR0113654A (en)
CA (1) CA2421215A1 (en)
IL (4) IL154759A0 (en)
WO (2) WO2002020812A1 (en)
ZA (1) ZA200301785B (en)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855864B2 (en) 2000-09-05 2005-02-15 Board Of Control Of Michigan Technological University Methods for simultaneous control of lignin content and composition, and cellulose content in plants
US7288696B2 (en) * 2000-09-05 2007-10-30 Board Of Control Of Michigan Technological University Genetic engineering of syringyl-enriched lignin in plants
EP1451327A4 (en) 2001-11-07 2006-04-05 Genesis Res & Dev Corp Ltd Compositions from the grasses lolium perenne and festuca arundinacea
SE0301233D0 (en) * 2003-04-28 2003-04-28 Swetree Technologies Ab Tissue specific promoters
CN1318579C (en) * 2003-08-08 2007-05-30 浙江省农业科学院 Gene engineering method for raising plant useful secondary substance content
EP2557169B1 (en) * 2004-04-06 2016-03-23 Fibria Celulose S/A Cambium/Xylem-preferred promoters and uses thereof
AU2015202438B2 (en) * 2004-04-06 2017-05-25 Fibria Celulose S/A Cambium/xylem-preferred promoters and uses thereof
US9238818B2 (en) * 2004-04-20 2016-01-19 Syngenta Participations Ag Methods and genetic constructs for modification of lignin composition of corn cobs
EP2366790A1 (en) * 2004-04-20 2011-09-21 Syngenta Participations AG Regulatory sequences for expressing gene products in plant reproductive tissue
WO2005108577A1 (en) * 2004-05-06 2005-11-17 Agriculture Victoria Services Pty Ltd Plant cad1-like genes and their use
US7663023B2 (en) 2004-07-24 2010-02-16 The Samuel Roberts Noble Foundation Modification of lignin biosynthesis
BRPI0514436C1 (en) 2004-08-18 2008-06-24 Alellyx Sa method to change wood density
US7456338B2 (en) 2004-09-22 2008-11-25 Arborgen Llc Modification of plant lignin content
US7799906B1 (en) 2004-09-22 2010-09-21 Arborgen, Llc Compositions and methods for modulating lignin of a plant
US7402428B2 (en) 2004-09-22 2008-07-22 Arborgen, Llc Modification of plant lignin content
GB0422977D0 (en) * 2004-10-15 2004-11-17 Swetree Technologies Ab Methods for improving tree growth and wood properties
EP2166100B1 (en) 2005-03-08 2012-07-18 BASF Plant Science GmbH Expression enhancing intron sequences
WO2006104891A2 (en) * 2005-03-28 2006-10-05 Syngenta Participations Ag Methods and genetic constructs for modification of lignin composition of corn cobs
WO2006119318A2 (en) * 2005-05-02 2006-11-09 Purdue Research Foundation Methods for increasing the yield of fermentable sugars from plant stover
AU2006303843B2 (en) * 2005-10-21 2012-08-09 Fibria Celulose S/A Polynucleotides, DNA constructs and methods for the alteration of plant cellulose content
US8183432B2 (en) * 2006-02-28 2012-05-22 Japan Science And Technology Agency Plant having reduced lignin and cellulose contents without reducing glucan content, method of producing the same and utilization thereof
US20070240837A1 (en) * 2006-04-13 2007-10-18 Andritz Inc. Hardwood alkaline pulping processes and systems
US8362322B2 (en) * 2006-10-27 2013-01-29 Ceres, Inc. Modulating lignin in plants
US9309528B2 (en) * 2006-11-21 2016-04-12 The Samuel Roberts Noble Foundation, Inc. Biofuel production methods and compositions
EP2118282A2 (en) * 2007-02-08 2009-11-18 BASF Plant Science GmbH Compositions and methods using rna interference of cad-like genes for control of nematodes
AU2007200824B2 (en) * 2007-02-23 2011-03-24 Fibria Celulose S/A Altering wood density
WO2008118385A2 (en) * 2007-03-23 2008-10-02 Michigan State University Lignin reduction and cellulose increase in crop biomass via genetic engineering
BRPI0910848A2 (en) 2008-04-28 2015-08-18 Univ Michigan Tech Fiber-specific promoter elements
MX2011013011A (en) 2009-06-05 2012-02-28 Univ Florida Isolation and targeted suppression of lignin biosynthetic genes from sugarcane.
US9334505B2 (en) 2011-08-12 2016-05-10 Purdue Research Foundation Using corngrass1 to engineer poplar as a bioenergy crop
US9556420B2 (en) * 2013-06-18 2017-01-31 Brookhaven Science Associates, Llc Specialized (iso)eugenol-4-O-methyltransferases (s-IEMTs) and methods of making and using the same
CN108467898B (en) * 2017-02-23 2021-07-27 北京林业大学 Method for screening poplar wood quality characters by jointly utilizing microRNA and SNP in target gene thereof
KR102198787B1 (en) * 2019-09-05 2021-01-05 한국과학기술연구원 Novel feed crop comprising mutated COMT gene and uses thereof
CN116548262B (en) * 2023-04-15 2023-11-03 西藏自治区农牧科学院草业科学研究所 Planting method for mixed sowing of oat and common vetch forage grass in alpine arid region

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4744026A (en) * 1986-04-11 1988-05-10 American Telephone And Telegraph Company, At&T Bell Laboratories Methods and apparatus for efficient resource allocation
US5295065A (en) * 1990-06-01 1994-03-15 Motorola, Inc. Resource-lot association coordinator
WO1992003905A2 (en) * 1990-08-31 1992-03-19 Ab Volvo A method and apparatus for optimally allocating resources
GB9109063D0 (en) * 1991-04-26 1991-06-12 Ici Plc Modification of lignin synthesis in plants
JPH05250377A (en) * 1992-03-04 1993-09-28 Fujitsu Ltd Scheduling system
GB9211416D0 (en) * 1992-05-29 1992-07-15 Ici Plc Expression of genes in transgenic plants
JP3551443B2 (en) * 1992-06-02 2004-08-04 王子製紙株式会社 Cinnamyl alcohol dehydrogenase gene of udo and hardwood, and hardwood into which the gene is introduced
US5630070A (en) * 1993-08-16 1997-05-13 International Business Machines Corporation Optimization of manufacturing resource planning
US5434775A (en) * 1993-11-04 1995-07-18 The General Hospital Corporation Managing an inventory of devices
FR2718460B1 (en) * 1994-04-11 1996-05-31 Centre Nat Rech Scient DNA sequence coding for cinnamoyl CoA reductase in Eucalyptus, and its applications in the field of regulation of plant lignin levels.
US6801820B1 (en) * 1994-05-27 2004-10-05 Lilly Software Associates, Inc. Method and apparatus for scheduling work orders in a manufacturing process
JP3315844B2 (en) * 1994-12-09 2002-08-19 株式会社東芝 Scheduling device and scheduling method
DE19535084A1 (en) * 1995-09-21 1997-03-27 Ibm Dynamic optimisation of business processes managed by computer system
US5922928A (en) * 1995-11-30 1999-07-13 Board Of Control Of Michigan Technological University Genetic transformation and regeneration of plants
AUPN773496A0 (en) * 1996-01-25 1996-02-15 Task Solutions Pty Ltd Task management system
US5754451A (en) * 1996-02-29 1998-05-19 Raytheon Company Preventative maintenance and diagonstic system
US5970437A (en) * 1996-10-03 1999-10-19 Gorman; Alexander J. Computerized management of plant maintenance and program therefor
US6252135B1 (en) 1996-12-16 2001-06-26 International Paper Company Production of syringyl lignin in gymnosperms
US5995915A (en) * 1997-01-29 1999-11-30 Advanced Micro Devices, Inc. Method and apparatus for the functional verification of digital electronic systems
WO1998053416A1 (en) * 1997-05-21 1998-11-26 Khimetrics, Inc. Method for controlled optimization of enterprise planning models
US6230200B1 (en) * 1997-09-08 2001-05-08 Emc Corporation Dynamic modeling for resource allocation in a file server
US6014633A (en) * 1997-09-24 2000-01-11 Deroyal Business Systems, L.L.C. Method for the analysis and standardization of bills of resources
US6096094A (en) * 1997-10-03 2000-08-01 National Instruments Corporation Configuration manager for configuring a data acquisition system
US6455762B1 (en) * 1997-11-12 2002-09-24 Board Of Control Of Michigan Technological University Methods of modifying lignin in plants by transformation with a 4-coumarate coenzyme a ligase nucleic acid
US6078912A (en) * 1998-04-15 2000-06-20 Travelhost, Inc. Computer-based system and method for resource determination and management
EP1006190A1 (en) * 1998-12-02 2000-06-07 Centrum Voor Plantenveredelings- En Reproduktieonderzoek Fruit flavour related genes and use thereof
AU777867B2 (en) * 1999-02-01 2004-11-04 Cellfor Inc. Modification of lignin composition of gymnosperms
US6067486A (en) * 1999-02-01 2000-05-23 General Electric Company Method and system for planning repair of an aircraft engine
US6321207B1 (en) * 1999-04-15 2001-11-20 I2 Technologies Us, Inc. System and method for optimizing the allocation of a resource
US7288409B1 (en) 1999-10-08 2007-10-30 Board Of Control Of Michigan Technological University Method of introducing a plurality of genes into plants
US7167786B2 (en) * 1999-12-01 2007-01-23 Sinex Aviation Technologies Corporation Dynamic management of aircraft part reliability data
US6678716B1 (en) * 2000-06-19 2004-01-13 J. D. Edwards World Source Company System and method for managing processes
US6496814B1 (en) * 2000-07-19 2002-12-17 International Business Machines Corporation Method and system for integrating spatial analysis, and scheduling to efficiently schedule and monitor infrastructure maintenance
CA2386080A1 (en) * 2000-08-07 2002-02-14 General Electric Company Computerized method and system for guiding service personnel to select a preferred work site for servicing transportation equipment
CA2419524A1 (en) 2000-08-16 2002-02-21 Purdue Research Foundation Genes encoding mutant p-coumarate 3-hydroxylase (c3h) and methods of use
US6855864B2 (en) 2000-09-05 2005-02-15 Board Of Control Of Michigan Technological University Methods for simultaneous control of lignin content and composition, and cellulose content in plants
US6738748B2 (en) * 2001-04-03 2004-05-18 Accenture Llp Performing predictive maintenance on equipment
US20020072988A1 (en) * 2000-12-13 2002-06-13 Itt Manufacturing Enterprises, Inc. Supply management system
WO2002054239A2 (en) * 2000-12-29 2002-07-11 General Electric Company Method and system for identifying repeatedly malfunctioning equipment
US6735549B2 (en) * 2001-03-28 2004-05-11 Westinghouse Electric Co. Llc Predictive maintenance display system
US20020143564A1 (en) * 2001-04-03 2002-10-03 Webb Brett M. Website for household inventory and maintenance with reminder system and method
US20020156692A1 (en) * 2001-04-20 2002-10-24 Squeglia Mark R. Method and system for managing supply of replacement parts of a piece of equipment
US6985803B2 (en) * 2001-05-30 2006-01-10 General Electric Company System and method for monitoring the condition of a vehicle
US20030036939A1 (en) * 2001-07-20 2003-02-20 Flores Abelardo A. Method and system configure to manage a maintenance process

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KAJITA, S. ET AL.: "Alterations in the biosynthesis of lignin in transgenic plants with chimeric genes for 4-Coumarate:Coenzyme A ligase.", PLANT CELL PHYSIOLOGY., vol. 37, no. 7, 1996, pages 957 - 965 *
OSAKABE, K. ET AL.: "Coniferyl aldehyde 5-hydroxylation and methylation direct syringlyl lignin biosynthesis in angiosperms.", PNAS., vol. 96, August 1999 (1999-08-01), pages 8955 - 8960 *

Also Published As

Publication number Publication date
WO2002020812A1 (en) 2002-03-14
AU8876501A (en) 2002-03-22
US20050166283A1 (en) 2005-07-28
US7514596B2 (en) 2009-04-07
US20020138870A1 (en) 2002-09-26
BR0113699A (en) 2004-06-08
WO2002020717A2 (en) 2002-03-14
AU2001288765C1 (en) 2002-03-22
AR030615A1 (en) 2003-08-27
IL154759A (en) 2009-08-03
ZA200301785B (en) 2004-03-04
AR030616A1 (en) 2003-08-27
BR0113654A (en) 2003-09-23
IL154760A0 (en) 2003-10-31
IL154759A0 (en) 2003-10-31
IL154760A (en) 2011-01-31
US20020124281A1 (en) 2002-09-05
CA2421215A1 (en) 2002-03-14
JP2004520015A (en) 2004-07-08
JP4871487B2 (en) 2012-02-08
AU2001288765B2 (en) 2006-04-27
US20020078474A1 (en) 2002-06-20
AU2001288716A1 (en) 2002-03-22
EP1320617A4 (en) 2005-01-19
AU2001288716A8 (en) 2009-07-30
US6855864B2 (en) 2005-02-15
JP2004515224A (en) 2004-05-27
EP1320617A1 (en) 2003-06-25
US6812377B2 (en) 2004-11-02
CN1473198A (en) 2004-02-04

Similar Documents

Publication Publication Date Title
WO2002020717A3 (en) Methods for simultaneous control of lignin content and composition, and cellulose content in plants
Chandler et al. Genetic modification in floriculture
Douglas Phenylpropanoid metabolism and lignin biosynthesis: from weeds to trees
Larkin et al. Increasing morphinan alkaloid production by over‐expressing codeinone reductase in transgenic Papaver somniferum
Boudet Lignins and lignification: selected issues
WO2003052073A3 (en) Novel corn event
WO2004009761A3 (en) Methods for using artificial polynucleotides and compositions thereof to reduce transgene silencing
WO1998049332A8 (en) Agrobacterium mediated transformation of sorghum
WO2002015675A8 (en) Genes for modifying plant traits iv
EP2295582A8 (en) Genes and uses for plant improvement
WO2002040694A3 (en) Heterologous expression of taxanes
AU1768201A (en) Seed trait genes
WO2000037663A3 (en) Plant transformation process
WO2001079472A3 (en) Nucleic acid sequences to proteins involved in tocopherol synthesis
Druege et al. Petunia as model for elucidating adventitious root formation and mycorrhizal symbiosis: at the nexus of physiology, genetics, microbiology and horticulture
Akita et al. Identification of functional flavonol synthase genes from fragrant wild cyclamen (Cyclamen purpurascens)
Liu et al. Genome-wide analysis of cytochrome P450 genes in Citrus clementina and characterization of a CYP gene encoding flavonoid 3′-hydroxylase
DE60119901D1 (en) TRANSGENIC TREES WITH INCREASED BIOMASS PRODUCTION AND XYLENE FIBER LENGTH AND METHOD OF MANUFACTURING THEM
Al-Ahmad Biotechnology for bioenergy dedicated trees: meeting future energy demands
WO2001085969A3 (en) Genetic transformation in plants using site-specific recombination and wide hybridization
Fan et al. Genome-wide investigation of Hydroxycinnamoyl CoA: Shikimate Hydroxycinnamoyl Transferase (HCT) gene family in Carthamus tinctorius L.
CA2320008A1 (en) Brassica transformation via microprojectile bombardment
Condliffe et al. An optimised protocol for rose transformation applicable to different cultivars
Kumar et al. Application of biotechnology in forestry: current status and future perspective
CN106047905A (en) Chalcone synthetase NtCHS1 gene of tobacco, and application thereof

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 154760

Country of ref document: IL

Ref document number: 2002525724

Country of ref document: JP

NENP Non-entry into the national phase

Ref country code: DE

WWE Wipo information: entry into national phase

Ref document number: 018184391

Country of ref document: CN

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase