WO2002034890A3 - Pluripotential stem cells - Google Patents

Pluripotential stem cells Download PDF

Info

Publication number
WO2002034890A3
WO2002034890A3 PCT/GB2001/004747 GB0104747W WO0234890A3 WO 2002034890 A3 WO2002034890 A3 WO 2002034890A3 GB 0104747 W GB0104747 W GB 0104747W WO 0234890 A3 WO0234890 A3 WO 0234890A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
somatic
lower relative
relative potency
derived
Prior art date
Application number
PCT/GB2001/004747
Other languages
French (fr)
Other versions
WO2002034890A2 (en
Inventor
Austin Gerrard Smith
Qi Long Ying
Original Assignee
Univ Edinburgh
Austin Gerrard Smith
Qi Long Ying
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Edinburgh, Austin Gerrard Smith, Qi Long Ying filed Critical Univ Edinburgh
Priority to AU2001295793A priority Critical patent/AU2001295793A1/en
Publication of WO2002034890A2 publication Critical patent/WO2002034890A2/en
Publication of WO2002034890A3 publication Critical patent/WO2002034890A3/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0271—Chimeric animals, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/02—Coculture with; Conditioned medium produced by embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/08—Coculture with; Conditioned medium produced by cells of the nervous system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/08—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from cells of the nervous system

Abstract

Described are mammalian stem cells derived from somatic stem cells of a lower relative potency, pluripotent stem cells are obtained from somatic stem cells of lower relative potency. Also described are mammalian stem cell lines that are capable of stable regeneration in vitro, which cell lines are derived from lower relative potency somatic stems cells. In addition, methods are provided for the production of mammalian stem cells, cell lines and non-human mammals derived from somatic stem cells of a lower relative potency. The invention also provides methods for reprogramming a nucleus of a somatic cell so as to become a less differentiated stem cell.
PCT/GB2001/004747 2000-10-26 2001-10-26 Pluripotential stem cells WO2002034890A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001295793A AU2001295793A1 (en) 2000-10-26 2001-10-26 Pluripotential stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0026252.7 2000-10-26
GB0026252A GB0026252D0 (en) 2000-10-26 2000-10-26 Pluripotential stem cells

Publications (2)

Publication Number Publication Date
WO2002034890A2 WO2002034890A2 (en) 2002-05-02
WO2002034890A3 true WO2002034890A3 (en) 2002-11-14

Family

ID=9902036

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/004747 WO2002034890A2 (en) 2000-10-26 2001-10-26 Pluripotential stem cells

Country Status (3)

Country Link
AU (1) AU2001295793A1 (en)
GB (1) GB0026252D0 (en)
WO (1) WO2002034890A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8252280B1 (en) 1999-08-05 2012-08-28 Regents Of The University Of Minnesota MAPC generation of muscle
US10638734B2 (en) 2004-01-05 2020-05-05 Abt Holding Company Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
US8609412B2 (en) 1999-08-05 2013-12-17 Regents Of The University Of Minnesota Mapc generation of lung tissue
EP2348104A1 (en) 1999-08-05 2011-07-27 Mcl Llc Multipotent adult stem cells and methods for isolation
US7015037B1 (en) 1999-08-05 2006-03-21 Regents Of The University Of Minnesota Multiponent adult stem cells and methods for isolation
RU2322497C2 (en) * 2000-11-30 2008-04-20 Стемрон Инк. Isolated homozygous stem cell (variants), method for its preparing (variants), method for preparing required cell-ancestor, differentiated cell, group of differentiated cells or tissue type
EP1367899A4 (en) 2001-02-14 2004-07-28 Leo T Furcht Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
US7422736B2 (en) * 2002-07-26 2008-09-09 Food Industry Research And Development Institute Somatic pluripotent cells
AU2003298016A1 (en) 2002-11-27 2004-06-23 Regents Of The University Of Minnesota Homologous recombination in multipotent adult progenitor cells
EP1941032A2 (en) 2005-10-14 2008-07-09 Regents Of The University Of Minnesota Differentiation of non-embryonic stem cells to cells having a pancreatic phenotype
US9005964B2 (en) 2006-11-24 2015-04-14 Regents Of The University Of Minnesota Endodermal progenitor cells
AU2009205886B2 (en) 2008-01-18 2015-08-27 Katholieke Universiteit Leuven Stem cell aggregates and methods for making and using
EP2362899A1 (en) 2008-10-31 2011-09-07 Katholieke Universiteit Leuven Optimized methods for differentiation of cells into cells with hepatocyte and hepatocyte progenitor phenotypes, cells produced by the methods, and methods for using the cells
SG10201503700WA (en) 2010-05-12 2015-06-29 Abt Holding Co Modulation of splenocytes in cell therapy
WO2011158125A2 (en) 2010-06-17 2011-12-22 Katholieke Universiteit Leuven Methods for differentiating cells into hepatic stellate cells and hepatic sinusoidal endothelial cells, cells produced by the methods, and methods for using the cells
CN103237886B (en) 2010-08-24 2018-10-30 明尼苏达大学董事会 The non-static suspension culture of cell aggregate
AU2014250761B2 (en) 2013-04-12 2019-02-28 Robert J. Deans Improving organs for transplantation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
WO2002024873A1 (en) * 2000-09-20 2002-03-28 Christopher Ralph Franks Stem cell therapy

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
WO2002024873A1 (en) * 2000-09-20 2002-03-28 Christopher Ralph Franks Stem cell therapy

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SVENDSEN C N & SMITH A G: "New prospects for human stem-cell therapy in the nervous system", TRENDS IN NEUROSCIENCE, vol. 22, no. 8, 1999, pages 357 - 364, XP001030096, ISSN: 0166-2236 *

Also Published As

Publication number Publication date
WO2002034890A2 (en) 2002-05-02
AU2001295793A1 (en) 2002-05-06
GB0026252D0 (en) 2000-12-13

Similar Documents

Publication Publication Date Title
WO2002034890A3 (en) Pluripotential stem cells
WO2001011011A3 (en) Multipotent adult stem cells and methods for isolation
WO2004044158A3 (en) Human embryonic stem cell cultures, and compositions and methods for growing same
WO2005116073A3 (en) Pdx1 expressing endoderm
WO2004055155A3 (en) Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
WO2004087896A3 (en) Hepatocyte differentiation of stem cells
WO2004070013A3 (en) Use of islet 1 as a marker for isolating or generating stem cells
WO2007051038A3 (en) Pdx1-expressing dorsal and ventral foregut endoderm
SG149061A1 (en) Definitive endoderm
WO2006047743A8 (en) Swine multipotent adult progenitor cells
IL148060A0 (en) Compositions and methods for improved cell culture
WO2002092756A8 (en) Insulin producing cells derived from human embryonic stem cells
MX2009014273A (en) Methods and compositions for optimized expansion and implantation of mesenchymal stem cells.
WO2006122147A3 (en) Alginate poly-l-lysine encapsulation as a technology for controlled differentiation of embryonic stem cells
NZ712789A (en) Placental stem cell populations
MXPA02003733A (en) Methods of producing differentiated progenitor cells and lineage-defective embryonic stem cells.
WO2007016366A3 (en) Defined culture conditions of human embryonic stem cells
WO2003004605A3 (en) Mesenchymal cells and osteoblasts from human embryonic stem cell
UA92157C2 (en) Process for production of growth hormone
HK1053327A1 (en) Method of culturing mesenchymal stem cells
WO2003016507A3 (en) Generation of multipotent central nervous system stem cells
WO2003024406A3 (en) Nestin-expressing hair follicle stem cells
WO2000070021A3 (en) Differentiated human embryoid cells and a method for producing them
MX2010001528A (en) Isolation, characterization and propagation of germline stem cells.
AU2019268061A1 (en) Placental stem cell populations

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
AK Designated states

Kind code of ref document: A3

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TR TT TZ UA UG US UZ VN YU ZA ZW

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP