WO2002042780A9 - Apparatus and methods for separating agglutinants and disperse particles - Google Patents
Apparatus and methods for separating agglutinants and disperse particlesInfo
- Publication number
- WO2002042780A9 WO2002042780A9 PCT/US2001/043373 US0143373W WO0242780A9 WO 2002042780 A9 WO2002042780 A9 WO 2002042780A9 US 0143373 W US0143373 W US 0143373W WO 0242780 A9 WO0242780 A9 WO 0242780A9
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- disc
- optical disc
- separation zone
- bioactive agent
- specificity
- Prior art date
Links
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Classifications
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Definitions
- This invention relates to separating agglutinants and disperse particles.
- agglutinated particles i.e., clumps of particles called "agglutinants"
- a specimen is deposited on a card of a contrasting color and is washed in a reagent.
- a visual check of the card is performed to determine whether clumping has formed.
- the biological sample material is allowed to react with the assay reagent to thereby facilitate formation of an agglutinant.
- the optical disc is rotated so that non-agglutinated particles escape from the entry chamber through the separation zone structure. Where the agglutinated particles remain, the disc is made to allow an interrogating light beam to be reflected from or transmitted past the particles to allow detection, imaging, and/or counting of the particles.
- FIG. 6 is a plan view of a bio-disc having one or more embodiments of microfluidic circuits and having optical reading tracks.
- FIG. 12 is a plan view of a bio-disc having one or more embodiments of microfluidic circuits.
- FIG. 13 is a plan view of a bio-disc having one or more embodiments of microfluidic circuits.
- An optical bio-disc for use with embodiments of the present invention may have any suitable shape, diameter, or thickness, but preferably is implemented on a round disc with a diameter and a thickness similar to those of a compact disc (CD), a recordable CD (CD-R), CD-RW, a digital versatile disc (DVD), DVD-R, DVD-RW, or other standard optical disc format.
- the disc may include encoded information, preferably in a known format, for performing, controlling, and post-processing a test or assay, such as information for controlling the rotation rate of the disc, timing for rotation, stopping and starting, delay periods, multiple rotation steps, locations of samples, position of the light source, and power of the light source. Such encoded information is referred to generally as operational information.
- the disc may be reflective, transmissive, or some combination of reflective and transmissive.
- a reflective disc an incident light beam is focused onto a reflective surface of the disc, reflected, and returned through optical elements to a detector on the same side of the disc as the light source.
- a transmissive disc light passes through the disc (or portions thereof) to a detector on the other side of the disc from the light source.
- some light may also be reflected and detected as reflected light.
- the light may include any type of electromagnetic radiation, such as visible light, infrared light, or ultraviolet light. Referring to FIGS.
- the investigational features captured by the capture layer may be designed to be located in the focal plane coplanar with reflective layer 148, where an incident beam is typically focused in conventional readers; alternatively, the investigational features may be captured in a plane spaced from the focal plane.
- the former configuration is referred to as a "proximal” type disc, and the latter a “distal” type disc.
- particles can be similarly detected by detecting whether or not an incident light beam is transmitted through corresponding tracks.
- the following program listing includes PASCAL source code for computer software routines that may be used to help select parameters for specific embodiments of the microfluidic circuits.
- the software routines perform particle agglutination modeling in which crosslinks are randomly introduced and the sizes of clumps of agglutinated particles are projected, which facilitates selection of spacing in the separation zone.
- the assay reagent or bioactive agent may include freeze-dried material, e.g., material that is freeze-dried into an area of the circuit, such as the entry chamber.
- the freeze-dried material may dissolve upon interaction with a sample or a specimen.
- An advantage of using freeze-dried material is that the disc need not be removed from and reinserted into the reader in an extra step solely for the purpose of introducing the assay reagent or bioactive agent.
- Another advantage of using freeze-dried material is that, in at least some cases, refrigeration and other preservation techniques and related equipment are unnecessary or less important, which renders at least some implementations of the invention more amenable to use in remote or resource-deprived locations or other places where preservation would otherwise be difficult or impossible.
- bioactive agent refers to any molecule A that recognizes a molecule B and binds with specificity thereto.
- binds with specificity is meant herein to refer to the binding of molecule A to molecule B to a significantly greater extent (e.g., by at least two fold, at least five fold, at least 10 fold, at least one hundred fold, or at least 1000 fold or more) relative to other molecules that may be present in a biological sample.
- molecules that specifically recognize and bind to other molecules include antibodies, ligands, receptors, enzymes, substrates, biotin, and avidin.
- the bioactive agent of the invention may be obtained from any source, including viral, bacterial, fungal, plant, animal, in vitro, or synthetically produced materials.
- the bioactive agent includes an antibody and the particle has at least one antibody bound thereto.
- antibody includes polyclonal, monoclonal, and recombinantly created antibodies. Antibodies of the invention can be produced in vivo or in vitro. Methods for the production of antibodies are well known to those skilled in the art. For example, see Antibody Production: Essential Techniques, Peter Delves (Ed.), John Wiley & Son Ltd, ISBN: 0471970107 (1997), which is incorporated herein in its entirely by reference. Alternatively, antibodies may be obtained from commercial sources, e.g., Research Diagnostics Inc., Pleasant Hill Road, Flanders, NJ 07836.
- a bioactive agent to be bound to a particle is within the skill of those in the art.
- a receptor-specific ligand may be bound to a particle for the purpose of agglutinating cells expressing the receptor recognized by the ligand or a particle may be bound by a lectin that binds specifically a sugar moiety expressed on the surface of a select population of cells for the purpose of agglutinating those cells.
- the particle with the bioactive agent bound thereto can be structured in any way suitable for the creation of agglutinants.
- one or more bioactive agents can be directly linked to the particle.
- particles may be uniformly bound with multiple copies of a single bioactive agent or, alternatively, particles may be bound with multiple copies of two or more bioactive agents to increase the specificity of the binding reaction or the occurrence of the subsequent agglutination reaction.
- the bioactive agent can be indirectly linked to the particle.
- a particle may be coated with a protein such as streptavidin and a bioactive agent such as an antibody can be linked to the streptavidin by way of a biotin moiety attached to the antibody.
- Viral Identification including Viral-related Identification
- FIG. 14 illustrates a bio-disc 1402 having a fluidic circuit 1410 that includes a U shaped main chamber 1412, first and second mixing chambers 1414, 1416, and first and second target areas 1418, 1420.
- First and second legs 1424, 1426 of a conduit 1422 connect main chamber 1412 with first and second mixing chamber 1414, 1416, respectively.
- Conduits 1428, 1430 connect first and second mixing chambers 1414, 1416 with first and second target areas 1418, 1420, respectively.
- Chamber 1412 and target areas 1418, 1420 have vents 1432, 1434, 1436, respectively.
- One or more of the separation structures may have openings 3-7 microns wide to allow red blood cells or other like-sized material to pass.
- the chamber may be shaped and sized to account for the expected proportions of the volumes of sample components to be retained in the zones. For example, in the case of a whole blood sample, it is expected that white blood cell volume will account for 1-2% of the sample volume and that red blood cell volume will account for 50% of the sample volume.
- the chamber may be narrower or smaller near center 1456 and accordingly may have a bottle, pyramid, or bell shape such that white blood cells may be retained in a small zone 1482 and red blood cells may be retained in a larger zone such as zone 1484 or 1486.
- An antibody having a specific color fluorescent tag that binds to neutrophils may be freeze-dried into the one of the zones 1482, 1484, 1486 that is expected to retain the neutrophils.
- a reader that can detect the specific fluorescent color can be used to quantitate or count the neutrophils that are retained in the zone, by means of the corresponding tracks 1493, 1494, 1495.
- a semi-quantitative result could be achieved by detecting an average fluorescent reading for an area, e.g., by detecting an overall amount of fluorescent glow in an area instead of counting fluorescent spots.
- both neutrophils and basophils may be quantitated, e.g., by using a green fluorescent tag for neutrophils and a red fluorescent tag for basophils.
- a reader that can detect and quantitate two colors could be used to produce a red-to-green ratio that is indicative of the ratio of basophils to neutrophils.
- the processed material is directed through legs 1424, 1426 to mixing chambers 1414, 1416, respectively.
- Mixing chambers 1414, 1416 have inlets 1460, 1462, respectively, through which additive material (e.g., A cells and B cells, respectively) may be added for mixing with the processed material.
- additive material e.g., A cells and B cells, respectively
- Agitation of the processed material with the additive material may be induced by rotating bio-disc 1402 back and forth at one or more speeds to produce mixtures. After the agitation, an increased speed of rotation, the mixtures are directed by centrifugal force through conduits 1428, 1430 to target areas 1418, 1420.
- the presence of cells or other results existing in target areas 1418, 1420 may be detected using incident light (e.g., laser) beams 1470, 1472 directed to the target areas, e.g., in accordance with the software of tracks 1450.
- a mass of agglutinated particles or a mass of disperse particles can be directed into a U shaped flow channel for hybridization or antigen antibody linking or another type of chemical reaction, and captured in a target area, and then either imaged or counted, which may aid determination of the precise volume of the agglutinated mass.
- a mass of agglutinated or dispersed particles may be directed from chamber to chamber by capillary action, by passage through appropriately sized channels, or by providing, in one or more instances, a breakaway seal in a channel or chamber such that rotation at an appropriately high speed causes breakage of the seal to allow the mass to be directed elsewhere for further processing.
- a bio-disc 1402 or a bio-disc based on aspects of bio-disc 1402 may be used in the performance or execution of assays and other procedures listed or referenced above, including serological assays, bacterial identification (including bacterial-related identification), viral identification (including viral-related identification), and amoebic identification.
Abstract
Description
Claims
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP01987031A EP1410045A2 (en) | 2000-11-22 | 2001-11-20 | Apparatus and methods for separating agglutinants and disperse particles |
AU2002239289A AU2002239289A1 (en) | 2000-11-22 | 2001-11-20 | Apparatus and methods for separating agglutinants and disperse particles |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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US25272500P | 2000-11-22 | 2000-11-22 | |
US60/252,725 | 2000-11-22 |
Publications (3)
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WO2002042780A2 WO2002042780A2 (en) | 2002-05-30 |
WO2002042780A3 WO2002042780A3 (en) | 2003-08-28 |
WO2002042780A9 true WO2002042780A9 (en) | 2004-03-04 |
Family
ID=22957264
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PCT/US2001/043373 WO2002042780A2 (en) | 2000-11-22 | 2001-11-20 | Apparatus and methods for separating agglutinants and disperse particles |
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Country | Link |
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US (1) | US20020196435A1 (en) |
EP (1) | EP1410045A2 (en) |
AU (1) | AU2002239289A1 (en) |
WO (1) | WO2002042780A2 (en) |
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2001
- 2001-11-20 AU AU2002239289A patent/AU2002239289A1/en not_active Abandoned
- 2001-11-20 EP EP01987031A patent/EP1410045A2/en not_active Withdrawn
- 2001-11-20 WO PCT/US2001/043373 patent/WO2002042780A2/en not_active Application Discontinuation
- 2001-11-20 US US09/989,684 patent/US20020196435A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
EP1410045A2 (en) | 2004-04-21 |
WO2002042780A3 (en) | 2003-08-28 |
AU2002239289A1 (en) | 2002-06-03 |
WO2002042780A2 (en) | 2002-05-30 |
US20020196435A1 (en) | 2002-12-26 |
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