WO2002044414A3 - Purification of dna sequencing reactions using silica magnetic particles - Google Patents

Purification of dna sequencing reactions using silica magnetic particles Download PDF

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Publication number
WO2002044414A3
WO2002044414A3 PCT/US2001/043364 US0143364W WO0244414A3 WO 2002044414 A3 WO2002044414 A3 WO 2002044414A3 US 0143364 W US0143364 W US 0143364W WO 0244414 A3 WO0244414 A3 WO 0244414A3
Authority
WO
WIPO (PCT)
Prior art keywords
magnetic particles
silica magnetic
sequencing reactions
dna sequencing
kit
Prior art date
Application number
PCT/US2001/043364
Other languages
French (fr)
Other versions
WO2002044414A2 (en
Inventor
Michael P Bjerke
Paul E Otto
Original Assignee
Promega Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Promega Corp filed Critical Promega Corp
Priority to EP01998656A priority Critical patent/EP1339876A2/en
Priority to AU2002217784A priority patent/AU2002217784A1/en
Publication of WO2002044414A2 publication Critical patent/WO2002044414A2/en
Publication of WO2002044414A3 publication Critical patent/WO2002044414A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Abstract

A method and kit are provided for using silica magnetic particles, preferably low porosity silica magnetic particles, to purify DNA extension products from DNA sequencing reactions containing unincorporated dideoxynucleotides and other materials in the solution likely to interfere with sequence analysis, prior to analysis. In a particularly preferred embodiment of the present invention, the method and kit have been designed for use in purifying DNA extension products from DNA sequencing reactions containing unincorporated dideoxynucleotides labeled with fluorescent dyes and other materials in the solution. DNA extension products purified from a sequencing reaction according to the present method or using the present kit produce highly accurate and long reads compared to reactions purified using other methods.
PCT/US2001/043364 2000-11-28 2001-11-21 Purification of dna sequencing reactions using silica magnetic particles WO2002044414A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP01998656A EP1339876A2 (en) 2000-11-28 2001-11-21 Purification of dna sequencing reactions using silica magnetic particles
AU2002217784A AU2002217784A1 (en) 2000-11-28 2001-11-21 Purification of dna sequencing reactions using silica magnetic particles

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US72416900A 2000-11-28 2000-11-28
US09/724,169 2000-11-28

Publications (2)

Publication Number Publication Date
WO2002044414A2 WO2002044414A2 (en) 2002-06-06
WO2002044414A3 true WO2002044414A3 (en) 2003-05-22

Family

ID=24909311

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/043364 WO2002044414A2 (en) 2000-11-28 2001-11-21 Purification of dna sequencing reactions using silica magnetic particles

Country Status (3)

Country Link
EP (1) EP1339876A2 (en)
AU (1) AU2002217784A1 (en)
WO (1) WO2002044414A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
GB0207495D0 (en) 2002-03-28 2002-05-08 Biochemie Gmbh Organic compounds
GB0215185D0 (en) 2002-07-01 2002-08-07 Genovision As Binding a target substance
WO2004020673A1 (en) * 2002-08-28 2004-03-11 Millipore Corporation Compositions of solution for sequencing reaction clean-up
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US7378260B2 (en) * 2005-04-01 2008-05-27 Applera Corporation Products and methods for reducing dye artifacts
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2015112914A1 (en) 2014-01-24 2015-07-30 Life Technologies Corporation Purification chemistries and formats for sanger dna sequencing reactions on a micro-fluidics device
CN107236726A (en) * 2017-05-23 2017-10-10 北京创新乐土基因科技有限公司 Clean CL purification kits and its application

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4233169A (en) * 1979-04-13 1980-11-11 Corning Glass Works Porous magnetic glass structure
US5683875A (en) * 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4233169A (en) * 1979-04-13 1980-11-11 Corning Glass Works Porous magnetic glass structure
US5683875A (en) * 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
US6027945A (en) * 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
US6194562B1 (en) * 1998-04-22 2001-02-27 Promega Corporation Endotoxin reduction in nucleic acid purification

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9540689B2 (en) 1998-05-01 2017-01-10 Life Technologies Corporation Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US9012144B2 (en) 2003-11-12 2015-04-21 Fluidigm Corporation Short cycle methods for sequencing polynucleotides

Also Published As

Publication number Publication date
AU2002217784A1 (en) 2002-06-11
EP1339876A2 (en) 2003-09-03
WO2002044414A2 (en) 2002-06-06

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