WO2002048164A3 - Isolation of nucleic acids - Google Patents

Isolation of nucleic acids Download PDF

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Publication number
WO2002048164A3
WO2002048164A3 PCT/GB2001/005524 GB0105524W WO0248164A3 WO 2002048164 A3 WO2002048164 A3 WO 2002048164A3 GB 0105524 W GB0105524 W GB 0105524W WO 0248164 A3 WO0248164 A3 WO 0248164A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
charge
ionisable group
isolation
nucleic acids
Prior art date
Application number
PCT/GB2001/005524
Other languages
French (fr)
Other versions
WO2002048164A2 (en
Inventor
Matthew John Baker
Original Assignee
Dna Res Innovations Ltd
Matthew John Baker
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dna Res Innovations Ltd, Matthew John Baker filed Critical Dna Res Innovations Ltd
Priority to DE60107468T priority Critical patent/DE60107468T2/en
Priority to AT01270542T priority patent/ATE283275T1/en
Priority to JP2002549695A priority patent/JP4868697B2/en
Priority to EP01270542A priority patent/EP1345952B1/en
Priority to CA002432075A priority patent/CA2432075A1/en
Priority to DK01270542T priority patent/DK1345952T3/en
Priority to AU2002216203A priority patent/AU2002216203A1/en
Publication of WO2002048164A2 publication Critical patent/WO2002048164A2/en
Publication of WO2002048164A3 publication Critical patent/WO2002048164A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Abstract

The present invention relates to materials for extracting nucleic acid using charge switch methods which involve, at a first pH, bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material; and releasing the nucleic acid at a second, higher, pH at which the charge on the material is negative, neutral or less positive. The release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a biological buffer, a polyhydroxylated amine, a detergent or surfactant, a carbohydrate, a nucleic acid base, a heterocyclic nitrogen-containing compound, a monoamine, a biological dye, or a negatively ionisable group, the pKa of which is between about 3.0 and 7.0 and a metal oxide which is positively charged at said first pH, and optionally also at said second pH.
PCT/GB2001/005524 2000-12-14 2001-12-14 Isolation of nucleic acids WO2002048164A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE60107468T DE60107468T2 (en) 2000-12-14 2001-12-14 NUCLEIC ACID ISOLATION
AT01270542T ATE283275T1 (en) 2000-12-14 2001-12-14 NUCLIC ACID ISOLATION
JP2002549695A JP4868697B2 (en) 2000-12-14 2001-12-14 Nucleic acid separation
EP01270542A EP1345952B1 (en) 2000-12-14 2001-12-14 Isolation of nucleic acids
CA002432075A CA2432075A1 (en) 2000-12-14 2001-12-14 Isolation of nucleic acids
DK01270542T DK1345952T3 (en) 2000-12-14 2001-12-14 Isolation of nucleic acids
AU2002216203A AU2002216203A1 (en) 2000-12-14 2001-12-14 Isolation of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/736,632 US6914137B2 (en) 1997-12-06 2000-12-14 Isolation of nucleic acids
US09/736,632 2000-12-14

Publications (2)

Publication Number Publication Date
WO2002048164A2 WO2002048164A2 (en) 2002-06-20
WO2002048164A3 true WO2002048164A3 (en) 2002-10-17

Family

ID=24960633

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/005524 WO2002048164A2 (en) 2000-12-14 2001-12-14 Isolation of nucleic acids

Country Status (10)

Country Link
US (8) US6914137B2 (en)
EP (2) EP1473299A3 (en)
JP (1) JP4868697B2 (en)
AT (1) ATE283275T1 (en)
AU (1) AU2002216203A1 (en)
CA (1) CA2432075A1 (en)
DE (1) DE60107468T2 (en)
DK (1) DK1345952T3 (en)
ES (1) ES2233560T3 (en)
WO (1) WO2002048164A2 (en)

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US20030054395A1 (en) 2003-03-20
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US20080305528A1 (en) 2008-12-11
AU2002216203A1 (en) 2002-06-24
US20030008320A1 (en) 2003-01-09
ATE283275T1 (en) 2004-12-15
US20010018513A1 (en) 2001-08-30
EP1473299A2 (en) 2004-11-03
US20130338245A1 (en) 2013-12-19
JP2004521881A (en) 2004-07-22
US20070231892A1 (en) 2007-10-04
ES2233560T3 (en) 2005-06-16
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US6914137B2 (en) 2005-07-05
EP1345952A2 (en) 2003-09-24
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EP1345952B1 (en) 2004-11-24
DE60107468D1 (en) 2004-12-30
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CA2432075A1 (en) 2002-06-20
WO2002048164A2 (en) 2002-06-20

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