WO2002062475B1 - Sample deposition method and system - Google Patents

Sample deposition method and system

Info

Publication number
WO2002062475B1
WO2002062475B1 PCT/US2002/001536 US0201536W WO02062475B1 WO 2002062475 B1 WO2002062475 B1 WO 2002062475B1 US 0201536 W US0201536 W US 0201536W WO 02062475 B1 WO02062475 B1 WO 02062475B1
Authority
WO
WIPO (PCT)
Prior art keywords
liquid droplet
sample plate
electric field
deposition system
liquid
Prior art date
Application number
PCT/US2002/001536
Other languages
French (fr)
Other versions
WO2002062475A1 (en
Inventor
Ansgar Brock
Christopher M Shaw
Robert C Downs
Original Assignee
Irm Llc
Ansgar Brock
Christopher M Shaw
Robert C Downs
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Irm Llc, Ansgar Brock, Christopher M Shaw, Robert C Downs filed Critical Irm Llc
Priority to DE60228049T priority Critical patent/DE60228049D1/en
Priority to EP02718848A priority patent/EP1358012B1/en
Priority to CA002434305A priority patent/CA2434305C/en
Priority to JP2002562474A priority patent/JP4162207B2/en
Publication of WO2002062475A1 publication Critical patent/WO2002062475A1/en
Publication of WO2002062475B1 publication Critical patent/WO2002062475B1/en
Priority to AU2007221973A priority patent/AU2007221973B2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0268—Drop counters; Drop formers using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00367—Pipettes capillary
    • B01J2219/00369—Pipettes capillary in multiple or parallel arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00371—Pipettes comprising electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00653—Making arrays on substantially continuous surfaces the compounds being bound to electrodes embedded in or on the solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00691—Automatic using robots
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/02—Drop detachment mechanisms of single droplets from nozzles or pins
    • B01L2400/027—Drop detachment mechanisms of single droplets from nozzles or pins electrostatic forces between substrate and tip
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Abstract

This invention provides methods and systems for rapidly, accurately and efficiently depositing liquid droplets (130) onto a planar uper surface (117) of sample plate (115) held in sample plate holder (110) which is supported on, or incorporated into, a motion table (105) included in an LC MALDI sample distribution system (100) for MALDI-mass spectrometry sample preparation. System (100) also includes a holding mechanism (118) for holding at least one capillary (125) which terminates at a capillary tip (127) on which droplet (130) is formed. A power supply (135) forms an electric field between sample plate surface (117) and droplet (130) which acts to pull the droplet (130) onto the sample plate (115).

Claims

AMENDED CLAIMS[Received by the International Bureau on 19 August 2002 ( 19.08.02): original claims 1-38 replaced by amended claims 1-41 ( 5 pages)]
1. A method of depositing a liquid droplet, the method comprising: providing a sample plate at a distance from a liquid droplet; and generating an electric field between the liquid droplet and the sample plate to polarize the liquid droplet, wherein the liquid droplet is either charged or grounded; wherein the liquid droplet is pulled to the sample plate along the electric field.
2. The method of claim 1, wherein providing a sample plate comprises moving the sample plate to a target position.
3. The method of claim 1 , wherein the liquid droplet is pulled to a target location on the sample plate.
4. The method of claim 1, wherein the liquid droplet is provided at a tip of a capillary.
5. The method of claim 4, wherein the capillary is connected to a column.
6. The method of claim 1, wherein generating an electric field comprises applying a charge to the liquid droplet.
7. The method of claim 6, wherein generating an electric field comprises connecting the sample plate to ground.
8. The method of claim 7, wherein the sample plate is connected to ground indirectly via an electrode plate that underlies the sample plate.
9. The method of claim 1, wherein generating an electric field comprises applying a charge to the sample plate.
10. The method of claim 9, wherein the charge is applied to the sample plate indirectly via an electrode plate that underlies the sample plate.
20
11. The method of claim 9, wherein generating an electric field comprises connecting the liquid droplet to ground.
12. The method of claim 1, wherein generating an electric field comprises applying a charge to one or more auxiliary electrodes.
13. The method of claim 1, wherein the distance between the liquid droplet and the sample plate is less than ten millimeters.
14. The method of claim 13, wherein the distance between the liquid droplet and the sample plate is approximately five millimeters.
15. The method of claim 1, wherein the electric field has a duration of 100- 300 milliseconds.
16. The method of claim 15, wherein the electric field has a duration of approximately 200 milliseconds.
17. The method of claim 1, wherein the electric field has a potential difference of 500-3000 volts.
18. The method of claim 17, wherein the potential difference is approximate!} 1000 volts.
19. The method of claim 1, wherein two or more drops are deposited at each target location on the sample plate.
20. The method of claim 1, wherein the liquid droplet comprises sample molecules.
21. The method of claim 1, wherein the liquid droplet comprises matrix molecules suitable for MALDI.
22. The method of claim 1, wherein the sample droplet comprises a mixture of sample molecules and matrix molecules.
23. A liquid droplet deposition system, comprising: a holding mechanism; a plurality of capillaries, held by the holding mechanism; a sample plate holder positionable with respect to the plurality of capillaries; and a power supply configured to generate an electric field between each capillary and a sample plate when the sample plate is placed upon the sample plate holder, along which electric field a droplet of liquid which can form at an end of the capillary is pulled to the sample plate.
24. The liquid droplet deposition system of claim 23, wherein each capillary comprises: a column for containing a liquid from which the liquid droplet is formed; and a capillary, connected at a first end to the column, and including an open tip at a second end for providing the droplets.
25. The liquid droplet deposition system of claim 23, wherein the column comprises a liquid chromatography column.
26. The liquid droplet deposition system of claim 23, wherein the sample plate holder is movable.
27. The liquid droplet deposition system of claim 23, wherein a sample plate is positioned on the sample plate holder below the array of capillaries.
28. The liquid droplet deposition system of claim 27, further comprising means for moving the sample plate to a target position.
29. The liquid droplet deposition system of claim 27, wherein the power supply includes a voltage source for applying a charge to the sample plate.
22
30. The liquid droplet deposition system of claim 29, wherein system comprises an electrode plate through which the charge is applied indirectly to the sample plate.
31. The liquid droplet deposition system of claim 29, wherein the system comprises an electrical connection which grounds a liquid droplet at an end of the capillary.
32. The liquid droplet deposition system of claim 23, wherein the power supply permits the independent application of a charge to each of a plurality of liquid droplets.
33. The liquid droplet deposition system of claim 23, wherein the power supply permits the independent application of a charge to different parts of the sample plate.
34. The liquid droplet deposition system of claim 33, wherein the power supply further includes a ground connection for grounding the liquid droplet.
35. The liquid droplet deposition system of claim 23, wherein the power supply includes a voltage source for applying a charge to the liquid droplet.
36. The liquid droplet deposition system of claim 23, further comprising a controller which controls one or more parameters selected from the group consisting of: movement of the sample plate, intensity of the electric field, duration of the electric field, and frequency of application of the electric field.
37. The liquid droplet deposition system of claim 36, wherein the controller comprises a computer which is programmed with software which provides a user interface which allows an operator to set parameters for the sample deposition.
38. The liquid droplet deposition system of claim 23, wherein the capillary is connected to a liquid chromatography column.
39. A liquid droplet deposition method, comprising:
23 providing a plurality of liquid droplets above a positionable sample plate; and attracting each droplet to a target location on the sample plate with an electric field formed between the plurality of droplets and the sample plate, wherein the liquid droplets are either charged or grounded.
40. The method of claim 39, wherein the plurality of liquid droplets includes eight or more droplets.
41. The method of claim 39, wherein attracting each droplet to a target location is performed in a succession to a single target location.
24
PCT/US2002/001536 2001-01-17 2002-01-17 Sample deposition method and system WO2002062475A1 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE60228049T DE60228049D1 (en) 2001-01-17 2002-01-17 SAMPLE STORAGE METHOD AND SYSTEM
EP02718848A EP1358012B1 (en) 2001-01-17 2002-01-17 Sample deposition method and system
CA002434305A CA2434305C (en) 2001-01-17 2002-01-17 Sample deposition method and system
JP2002562474A JP4162207B2 (en) 2001-01-17 2002-01-17 Sample mounting method and system
AU2007221973A AU2007221973B2 (en) 2001-01-17 2007-10-12 Sample deposition method and system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/765,207 2001-01-17
US09/765,207 US6918309B2 (en) 2001-01-17 2001-01-17 Sample deposition method and system

Publications (2)

Publication Number Publication Date
WO2002062475A1 WO2002062475A1 (en) 2002-08-15
WO2002062475B1 true WO2002062475B1 (en) 2002-11-21

Family

ID=25072933

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/001536 WO2002062475A1 (en) 2001-01-17 2002-01-17 Sample deposition method and system

Country Status (8)

Country Link
US (2) US6918309B2 (en)
EP (1) EP1358012B1 (en)
JP (1) JP4162207B2 (en)
AT (1) ATE403496T1 (en)
AU (1) AU2007221973B2 (en)
CA (1) CA2434305C (en)
DE (1) DE60228049D1 (en)
WO (1) WO2002062475A1 (en)

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Also Published As

Publication number Publication date
US7578206B2 (en) 2009-08-25
EP1358012A4 (en) 2006-04-05
CA2434305C (en) 2010-03-09
ATE403496T1 (en) 2008-08-15
US6918309B2 (en) 2005-07-19
EP1358012B1 (en) 2008-08-06
AU2007221973B2 (en) 2009-12-10
US20050232823A1 (en) 2005-10-20
JP4162207B2 (en) 2008-10-08
EP1358012A1 (en) 2003-11-05
WO2002062475A1 (en) 2002-08-15
DE60228049D1 (en) 2008-09-18
JP2004529325A (en) 2004-09-24
CA2434305A1 (en) 2002-08-15
AU2007221973A1 (en) 2007-11-01
US20020092366A1 (en) 2002-07-18

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