WO2002074055A2 - Epifluorecence microscope with improved image contrast - Google Patents
Epifluorecence microscope with improved image contrast Download PDFInfo
- Publication number
- WO2002074055A2 WO2002074055A2 PCT/US2002/008646 US0208646W WO02074055A2 WO 2002074055 A2 WO2002074055 A2 WO 2002074055A2 US 0208646 W US0208646 W US 0208646W WO 02074055 A2 WO02074055 A2 WO 02074055A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- excitation light
- light
- emission
- dichroic
- Prior art date
Links
- 230000005284 excitation Effects 0.000 claims abstract description 101
- 239000000523 sample Substances 0.000 claims description 121
- 238000003384 imaging method Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 5
- 238000000386 microscopy Methods 0.000 claims description 5
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 claims description 4
- 239000010936 titanium Substances 0.000 claims description 4
- 229910052719 titanium Inorganic materials 0.000 claims description 4
- 239000012472 biological sample Substances 0.000 claims description 2
- 238000001317 epifluorescence microscopy Methods 0.000 abstract 1
- 239000010408 film Substances 0.000 description 25
- 239000000203 mixture Substances 0.000 description 8
- 238000004061 bleaching Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 229910052751 metal Inorganic materials 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 238000004544 sputter deposition Methods 0.000 description 4
- 238000005286 illumination Methods 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000001755 magnetron sputter deposition Methods 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000000399 optical microscopy Methods 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000005289 physical deposition Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/082—Condensers for incident illumination only
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
Description
Claims
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP02753676A EP1405058A4 (en) | 2001-03-19 | 2002-03-19 | System and method for increasing the contrast of an image produced by an epifluorescence microscope |
CA002441878A CA2441878A1 (en) | 2001-03-19 | 2002-03-19 | System and method for increasing the contrast of an image produced by an epifluorescence microscope |
AU2002306790A AU2002306790B2 (en) | 2001-03-19 | 2002-03-19 | Epifluorescence microscope with improved image contrast |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US27690601P | 2001-03-19 | 2001-03-19 | |
US60/276,906 | 2001-03-19 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002074055A2 true WO2002074055A2 (en) | 2002-09-26 |
WO2002074055A3 WO2002074055A3 (en) | 2004-01-15 |
Family
ID=23058579
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/008646 WO2002074055A2 (en) | 2001-03-19 | 2002-03-19 | Epifluorecence microscope with improved image contrast |
Country Status (5)
Country | Link |
---|---|
US (4) | US6956695B2 (en) |
EP (1) | EP1405058A4 (en) |
AU (1) | AU2002306790B2 (en) |
CA (1) | CA2441878A1 (en) |
WO (1) | WO2002074055A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6956695B2 (en) | 2001-03-19 | 2005-10-18 | Ikonisys, Inc. | System and method for increasing the contrast of an image produced by an epifluorescence microscope |
WO2018065649A1 (en) * | 2016-10-03 | 2018-04-12 | Consejo Superior De Investigaciones Científicas (Csic) | Use of a material for the production of a cover slip, a sample holder or a cell culture container |
WO2019233935A1 (en) * | 2018-06-04 | 2019-12-12 | Jenoptik Optical Systems Gmbh | Microscope and method for capturing a microscopic image and use of a planar reflector |
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JP2004188422A (en) * | 2002-12-06 | 2004-07-08 | Hamamatsu Photonics Kk | Device and method for machining laser beam |
JP4434649B2 (en) * | 2003-03-27 | 2010-03-17 | 株式会社Eci | Observation instrument and observation method using the same |
FR2942319B1 (en) * | 2009-02-13 | 2011-03-18 | Novacyt | PROCESS FOR PREPARING A PROCESSED VIRTUAL ANALYSIS PLATE |
EP2384686B8 (en) * | 2009-04-21 | 2013-01-16 | Olympus Medical Systems Corp. | Fluorescence image device and fluorescence image acquiring method |
EP2465002A4 (en) * | 2009-08-10 | 2013-03-27 | Chroma Technology Corp | Microscope cube |
US20120301872A1 (en) * | 2009-11-20 | 2012-11-29 | Ge Healthcare Bio-Sciences Ab | System and method for increased fluorescence detection |
US8696991B1 (en) | 2010-01-04 | 2014-04-15 | Harold W. Howe | Field deployable surface plasmon resonance based biosensor |
US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
JP2013524214A (en) * | 2010-03-29 | 2013-06-17 | アナロジック コーポレイション | Photodetection system and / or photodetection method |
WO2013015734A1 (en) * | 2011-07-24 | 2013-01-31 | Applied Presicion, Inc. | Fluorescence microscopes with polychroic mirror changers |
US8687180B2 (en) | 2012-06-07 | 2014-04-01 | Molecular Devices, Llc | System, method, and device for determining a focal position of an objective in a microscopy imaging system |
EP3251578A1 (en) * | 2016-05-30 | 2017-12-06 | Leica Instruments (Singapore) Pte. Ltd. | Medical device for the observation of a partly fluorescent object, using a filter system with a transmission window |
EP3465145A2 (en) * | 2016-06-02 | 2019-04-10 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Configurable retro-reflective sensor system for the improved characterization of the properties of a sample |
US11175222B2 (en) * | 2017-11-15 | 2021-11-16 | The Board Of Trustees Of The University Of Illinois | Integrated spectroscopic analysis system with low vertical height for measuring liquid or solid assays |
DE102018212489A1 (en) * | 2018-07-26 | 2020-01-30 | Implen GmbH | Device for light spectroscopic analysis |
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US6388788B1 (en) * | 1998-03-16 | 2002-05-14 | Praelux, Inc. | Method and apparatus for screening chemical compounds |
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-
2002
- 2002-03-19 WO PCT/US2002/008646 patent/WO2002074055A2/en active IP Right Grant
- 2002-03-19 US US10/102,500 patent/US6956695B2/en not_active Expired - Fee Related
- 2002-03-19 EP EP02753676A patent/EP1405058A4/en not_active Withdrawn
- 2002-03-19 AU AU2002306790A patent/AU2002306790B2/en not_active Ceased
- 2002-03-19 CA CA002441878A patent/CA2441878A1/en not_active Abandoned
-
2005
- 2005-09-13 US US11/225,460 patent/US7330309B2/en not_active Expired - Fee Related
-
2008
- 2008-01-07 US US11/970,198 patent/US20080106743A1/en not_active Abandoned
- 2008-01-07 US US11/970,189 patent/US20080100911A1/en not_active Abandoned
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Title |
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See also references of EP1405058A2 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6956695B2 (en) | 2001-03-19 | 2005-10-18 | Ikonisys, Inc. | System and method for increasing the contrast of an image produced by an epifluorescence microscope |
US7330309B2 (en) | 2001-03-19 | 2008-02-12 | Ikonisys, Inc. | System and method for increasing the contrast of an image produced by an epifluorescence microscope |
WO2018065649A1 (en) * | 2016-10-03 | 2018-04-12 | Consejo Superior De Investigaciones Científicas (Csic) | Use of a material for the production of a cover slip, a sample holder or a cell culture container |
WO2019233935A1 (en) * | 2018-06-04 | 2019-12-12 | Jenoptik Optical Systems Gmbh | Microscope and method for capturing a microscopic image and use of a planar reflector |
Also Published As
Publication number | Publication date |
---|---|
US20020141050A1 (en) | 2002-10-03 |
US7330309B2 (en) | 2008-02-12 |
US20060056016A1 (en) | 2006-03-16 |
CA2441878A1 (en) | 2002-09-26 |
WO2002074055A3 (en) | 2004-01-15 |
US20080106743A1 (en) | 2008-05-08 |
EP1405058A4 (en) | 2007-07-04 |
US20080100911A1 (en) | 2008-05-01 |
US6956695B2 (en) | 2005-10-18 |
EP1405058A2 (en) | 2004-04-07 |
AU2002306790B2 (en) | 2007-05-24 |
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