WO2002074055A3 - Epifluorecence microscope with improved image contrast - Google Patents

Epifluorecence microscope with improved image contrast Download PDF

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Publication number
WO2002074055A3
WO2002074055A3 PCT/US2002/008646 US0208646W WO02074055A3 WO 2002074055 A3 WO2002074055 A3 WO 2002074055A3 US 0208646 W US0208646 W US 0208646W WO 02074055 A3 WO02074055 A3 WO 02074055A3
Authority
WO
WIPO (PCT)
Prior art keywords
epifluorecence
microscope
image contrast
improved image
sample
Prior art date
Application number
PCT/US2002/008646
Other languages
French (fr)
Other versions
WO2002074055A2 (en
Inventor
Triantafyllos Tafas
Petros Tsipouras
Original Assignee
Ikonisys Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ikonisys Inc filed Critical Ikonisys Inc
Priority to AU2002306790A priority Critical patent/AU2002306790B2/en
Priority to CA002441878A priority patent/CA2441878A1/en
Priority to EP02753676A priority patent/EP1405058A4/en
Publication of WO2002074055A2 publication Critical patent/WO2002074055A2/en
Publication of WO2002074055A3 publication Critical patent/WO2002074055A3/en

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/082—Condensers for incident illumination only
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides

Abstract

The contrast of an image produced by epifluorescence microscopy may be increased by placing a high-pass dichroic reflecting film (207) behind the sample (200). The reflecting film (207) reflects the emission light emitted by the fluorescent tags in the sample (200) back through the objective lens (240) while allowing the shorter wavelength excitation light to pass through the sample holder (205).
PCT/US2002/008646 2001-03-19 2002-03-19 Epifluorecence microscope with improved image contrast WO2002074055A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
AU2002306790A AU2002306790B2 (en) 2001-03-19 2002-03-19 Epifluorescence microscope with improved image contrast
CA002441878A CA2441878A1 (en) 2001-03-19 2002-03-19 System and method for increasing the contrast of an image produced by an epifluorescence microscope
EP02753676A EP1405058A4 (en) 2001-03-19 2002-03-19 System and method for increasing the contrast of an image produced by an epifluorescence microscope

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27690601P 2001-03-19 2001-03-19
US60/276,906 2001-03-19

Publications (2)

Publication Number Publication Date
WO2002074055A2 WO2002074055A2 (en) 2002-09-26
WO2002074055A3 true WO2002074055A3 (en) 2004-01-15

Family

ID=23058579

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/008646 WO2002074055A2 (en) 2001-03-19 2002-03-19 Epifluorecence microscope with improved image contrast

Country Status (5)

Country Link
US (4) US6956695B2 (en)
EP (1) EP1405058A4 (en)
AU (1) AU2002306790B2 (en)
CA (1) CA2441878A1 (en)
WO (1) WO2002074055A2 (en)

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JP4689767B2 (en) * 2009-04-21 2011-05-25 オリンパスメディカルシステムズ株式会社 Fluorescence imaging apparatus and method of operating fluorescence imaging apparatus
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Also Published As

Publication number Publication date
WO2002074055A2 (en) 2002-09-26
US20020141050A1 (en) 2002-10-03
US20080100911A1 (en) 2008-05-01
US20060056016A1 (en) 2006-03-16
EP1405058A4 (en) 2007-07-04
US20080106743A1 (en) 2008-05-08
US7330309B2 (en) 2008-02-12
EP1405058A2 (en) 2004-04-07
AU2002306790B2 (en) 2007-05-24
CA2441878A1 (en) 2002-09-26
US6956695B2 (en) 2005-10-18

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