WO2002082046A3 - Silicon-wafer based devices and methods for analyzing biological material - Google Patents

Silicon-wafer based devices and methods for analyzing biological material Download PDF

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Publication number
WO2002082046A3
WO2002082046A3 PCT/US2002/010673 US0210673W WO02082046A3 WO 2002082046 A3 WO2002082046 A3 WO 2002082046A3 US 0210673 W US0210673 W US 0210673W WO 02082046 A3 WO02082046 A3 WO 02082046A3
Authority
WO
WIPO (PCT)
Prior art keywords
pore
cell
silicon
methods
biological material
Prior art date
Application number
PCT/US2002/010673
Other languages
French (fr)
Other versions
WO2002082046A2 (en
Inventor
James R Heath
Rigo Pantoja
Francisco Bezanilla
Daniel M Sigg
Original Assignee
Univ California
James R Heath
Rigo Pantoja
Francisco Bezanilla
Daniel M Sigg
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, James R Heath, Rigo Pantoja, Francisco Bezanilla, Daniel M Sigg filed Critical Univ California
Priority to AU2002338371A priority Critical patent/AU2002338371A1/en
Priority to US10/473,784 priority patent/US20040120854A1/en
Publication of WO2002082046A2 publication Critical patent/WO2002082046A2/en
Publication of WO2002082046A3 publication Critical patent/WO2002082046A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing

Abstract

A semi-conductor wafer-based device that is used to suspend biological materials, such as lipid bilayers or single cells, for chemical, electrical and/or optical examination. The wafer (30) has at least one pore (32) that extends therethrough. The pore is of sufficient size to suspend a lipid bilayer (41) or a cell (20) therein. The surface of the pore is coated with an insulating film (31) to provide an insulating surface to which the lipid bilayer/cell is attached when the lipid bilayer/cell is suspended within the pore. The divice is used to measure the physical properties (e.g., voltage gating) of cells and lipid bilayers that contain biomolecules such as transmembrane proteins.
PCT/US2002/010673 2001-04-06 2002-04-04 Silicon-wafer based devices and methods for analyzing biological material WO2002082046A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002338371A AU2002338371A1 (en) 2001-04-06 2002-04-04 Silicon-wafer based devices and methods for analyzing biological material
US10/473,784 US20040120854A1 (en) 2002-04-04 2002-04-04 Silicon-wafer based devices and methods for analyzing biological material

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US28200501P 2001-04-06 2001-04-06
US60/282,005 2001-04-06

Publications (2)

Publication Number Publication Date
WO2002082046A2 WO2002082046A2 (en) 2002-10-17
WO2002082046A3 true WO2002082046A3 (en) 2003-03-20

Family

ID=23079679

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/010673 WO2002082046A2 (en) 2001-04-06 2002-04-04 Silicon-wafer based devices and methods for analyzing biological material

Country Status (2)

Country Link
AU (1) AU2002338371A1 (en)
WO (1) WO2002082046A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2447043A (en) * 2007-02-20 2008-09-03 Oxford Nanolabs Ltd Lipid bilayer sensor system
US20110121840A1 (en) 2007-02-20 2011-05-26 Gurdial Singh Sanghera Lipid Bilayer Sensor System
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
GB201202519D0 (en) 2012-02-13 2012-03-28 Oxford Nanopore Tech Ltd Apparatus for supporting an array of layers of amphiphilic molecules and method of forming an array of layers of amphiphilic molecules
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
GB201418512D0 (en) 2014-10-17 2014-12-03 Oxford Nanopore Tech Ltd Electrical device with detachable components
GB201611770D0 (en) 2016-07-06 2016-08-17 Oxford Nanopore Tech Microfluidic device
EP3938779A1 (en) 2019-03-12 2022-01-19 Oxford Nanopore Technologies Limited Nanopore sensing device and methods of operation and of forming it

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4706493A (en) * 1985-12-13 1987-11-17 General Motors Corporation Semiconductor gas sensor having thermally isolated site
US5601982A (en) * 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US5955377A (en) * 1991-02-11 1999-09-21 Biostar, Inc. Methods and kits for the amplification of thin film based assays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4706493A (en) * 1985-12-13 1987-11-17 General Motors Corporation Semiconductor gas sensor having thermally isolated site
US5955377A (en) * 1991-02-11 1999-09-21 Biostar, Inc. Methods and kits for the amplification of thin film based assays
US5601982A (en) * 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides

Also Published As

Publication number Publication date
AU2002338371A1 (en) 2002-10-21
WO2002082046A2 (en) 2002-10-17

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