WO2002090539A3 - Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases - Google Patents

Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases Download PDF

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Publication number
WO2002090539A3
WO2002090539A3 PCT/US2001/045071 US0145071W WO02090539A3 WO 2002090539 A3 WO2002090539 A3 WO 2002090539A3 US 0145071 W US0145071 W US 0145071W WO 02090539 A3 WO02090539 A3 WO 02090539A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acids
compositions
methods
isolating nucleic
kits
Prior art date
Application number
PCT/US2001/045071
Other languages
French (fr)
Other versions
WO2002090539A2 (en
Inventor
I Larry Greenfeld
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=24911121&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO2002090539(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Applera Corp filed Critical Applera Corp
Priority to AU2001297835A priority Critical patent/AU2001297835B2/en
Priority to DE60142254T priority patent/DE60142254D1/en
Priority to CA002429941A priority patent/CA2429941A1/en
Priority to EP01274041A priority patent/EP1354036B1/en
Priority to JP2002587600A priority patent/JP2005501523A/en
Priority to AT01274041T priority patent/ATE469219T1/en
Publication of WO2002090539A2 publication Critical patent/WO2002090539A2/en
Publication of WO2002090539A3 publication Critical patent/WO2002090539A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Abstract

The invention relates to compositions and methods for isolating nucleic acids from biological samples, including whole tissue. The invention also provides kits for isolating nucleic acids from biological samples.
PCT/US2001/045071 2000-11-28 2001-11-28 Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases WO2002090539A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
AU2001297835A AU2001297835B2 (en) 2000-11-28 2001-11-28 Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
DE60142254T DE60142254D1 (en) 2000-11-28 2001-11-28 COMPOSITIONS, METHODS AND REAGENTS FOR NUCLEIC ACID INSULATION USING SURFACE-ACTIVE SUBSTANCES AND PROTEASES
CA002429941A CA2429941A1 (en) 2000-11-28 2001-11-28 Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
EP01274041A EP1354036B1 (en) 2000-11-28 2001-11-28 Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
JP2002587600A JP2005501523A (en) 2000-11-28 2001-11-28 Compositions, methods and kits for isolating nucleic acids using detergents and proteases
AT01274041T ATE469219T1 (en) 2000-11-28 2001-11-28 COMPOSITIONS, METHODS AND REAGENTS FOR NUCLEIC ACID ISOLATION USING SURFACE-ACTIVE SUBSTANCES AND PROTEASES

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/724,613 2000-11-28
US09/724,613 US7001724B1 (en) 2000-11-28 2000-11-28 Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases

Publications (2)

Publication Number Publication Date
WO2002090539A2 WO2002090539A2 (en) 2002-11-14
WO2002090539A3 true WO2002090539A3 (en) 2003-08-07

Family

ID=24911121

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/045071 WO2002090539A2 (en) 2000-11-28 2001-11-28 Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases

Country Status (8)

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US (3) US7001724B1 (en)
EP (1) EP1354036B1 (en)
JP (2) JP2005501523A (en)
AT (1) ATE469219T1 (en)
AU (1) AU2001297835B2 (en)
CA (1) CA2429941A1 (en)
DE (1) DE60142254D1 (en)
WO (1) WO2002090539A2 (en)

Families Citing this family (144)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
DE10105912A1 (en) * 2001-02-09 2002-08-14 Roche Diagnostics Gmbh Recombinant Proteinase K
WO2002086492A1 (en) * 2001-04-17 2002-10-31 Chugai Seiyaku Kabushiki Kaisha Method of quantifying surfactant
AU2008201675B2 (en) * 2001-05-14 2012-06-07 Zygem Corporation Limited Thermostable Proteinases From Thermophilic Bacteria
NZ511680A (en) * 2001-05-14 2004-07-30 Univ Waikato Method for preparing nucleic acid or DNA samples and a DNA extraction process using thermophilic proteases
US7147826B2 (en) * 2001-10-12 2006-12-12 Becton Dickinson And Company Method and apparatus for transporting biological samples
JP4106026B2 (en) 2001-11-28 2008-06-25 アプレラ コーポレイション Selective nucleic acid isolation methods and compositions
DE60330842D1 (en) 2002-01-28 2010-02-25 Ambion Inc FOR THE DETECTION OF NUCLEIC ACIDS, COMPETENT BIODEGRADES
GB0212825D0 (en) * 2002-05-31 2002-07-10 Dna Res Innovations Ltd Methods compositions and kits for cell separation
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
US7998699B2 (en) 2002-08-15 2011-08-16 University Of South Florida Early detection of pathogens in blood
CN1332972C (en) * 2002-11-28 2007-08-22 爱科来株式会社 Method and apparatus for concentration and purification of nucleic acid
EP1568705B1 (en) * 2002-11-28 2008-10-29 Arkray Inc. Method and apparatus for concentration and purification of nucleic acid
US20040157219A1 (en) * 2003-02-06 2004-08-12 Jianrong Lou Chemical treatment of biological samples for nucleic acid extraction and kits therefor
US7601491B2 (en) * 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
JP4579234B2 (en) 2003-03-10 2010-11-10 エクスプレッション、パソロジー、インコーポレイテッド Liquid tissue preparation from histopathologically processed biological samples, tissues and cells
JPWO2004094634A1 (en) * 2003-04-22 2006-07-13 アークレイ株式会社 Nucleic acid isolation method and kit and apparatus for nucleic acid isolation
US20050009036A1 (en) * 2003-07-11 2005-01-13 Applera Corporation Methods and kits for obtaining nucleic acid from biological samples
PT2103694E (en) * 2003-08-15 2011-07-26 Univ South Florida Materials and methods for capture of pathogens and removal of aurintricarboxylic acid from a sample
EP1529840A1 (en) * 2003-11-04 2005-05-11 Qiagen GmbH A rapid and low cost method for isolating nucleic acid
AU2005200670B2 (en) 2004-02-20 2007-05-03 F. Hoffmann-La Roche Ag Adsorption of nucleic acids to a solid phase
US20050239091A1 (en) * 2004-04-23 2005-10-27 Collis Matthew P Extraction of nucleic acids using small diameter magnetically-responsive particles
US7593483B2 (en) * 2004-05-07 2009-09-22 Broadcom Corporation Nonlinear mapping in digital-to-analog and analog-to-digital converters
EP2290071B1 (en) 2004-05-28 2014-12-31 Asuragen, Inc. Methods and compositions involving microRNA
SG178651A1 (en) * 2004-06-17 2012-03-29 Zygem Corp Ltd Purification methods and uses thereof
DE102004034433A1 (en) * 2004-07-15 2006-02-02 Qiagen Gmbh Process for purifying and isolating nucleic acids using cationic detergents
JP2008511816A (en) * 2004-08-03 2008-04-17 ベクトン・ディキンソン・アンド・カンパニー Use of magnetic materials to separate samples
AU2005271688B2 (en) * 2004-08-03 2011-10-06 Becton, Dickinson And Company Use of magnetic material to direct isolation of compounds and fractionation of multipart samples
JP2006087394A (en) * 2004-09-27 2006-04-06 Wako Pure Chem Ind Ltd Method for extracting nucleic acid and kit for extracting nucleic acid
GB2419594C (en) * 2004-11-02 2011-03-02 Ind Tecnology Res Inst Method for stabilizing or isolating nucleic acids.
TWI294460B (en) * 2004-12-23 2008-03-11 Ind Tech Res Inst Method for stabilizing nucleic acids
EP2281887A1 (en) 2004-11-12 2011-02-09 Asuragen, Inc. Methods and compositions involving miRNA and miRNA inhibitor molecules
US7226875B2 (en) * 2004-11-30 2007-06-05 Taiwan Semiconductor Manufacturing Co., Ltd. Method for enhancing FSG film stability
CN1796398B (en) * 2004-12-29 2012-09-26 财团法人工业技术研究院 Method for extracting nucleic acid by using activator with amine interface
US20060188892A1 (en) * 2005-02-18 2006-08-24 Ambion, Inc. Enzymatic digestion of tissue
EP1910389A4 (en) * 2005-05-31 2010-03-10 Life Technologies Corp Separation and purification of nucleic acid from paraffin-containing samples
WO2007002567A2 (en) * 2005-06-23 2007-01-04 Nanosphere, Inc. Selective isolation and concentration of nucleic acids from complex samples
US20070015165A1 (en) * 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
JP5627162B2 (en) * 2005-10-05 2014-11-19 倉敷紡績株式会社 Nucleic acid separation and purification method and nucleic acid separation kit using the method
US20100216657A1 (en) * 2006-05-16 2010-08-26 Arcxis Biotechnologies, Inc. Pcr-free sample preparation and detection systems for high speed biologic analysis and identification
JP2009545317A (en) * 2006-08-01 2009-12-24 アプライド バイオシステムズ， エルエルシー Analyte and nucleic acid detection
US20120107799A1 (en) * 2010-10-29 2012-05-03 Longhorn Vaccines & Diagnostics LLC. Disposable, rapid extraction apparatus and methods
JP2010510964A (en) * 2006-09-19 2010-04-08 アシュラジェン インコーポレイテッド MiR-15, miR-26, miR-31, miR-145, miR-147, miR-188, miR-215, miR-216, miR-331, mmu-miR-292 as targets for therapeutic intervention Genes and pathways regulated by 3p
NZ576003A (en) * 2006-10-06 2012-03-30 Dna Genotek Inc Stabilizing compositions and methods for extraction of ribonucleic acid
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
CA2671194A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-20 regulated genes and pathways as targets for therapeutic intervention
CA2671294A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Mir-21 regulated genes and pathways as targets for therapeutic intervention
AU2007333109A1 (en) * 2006-12-08 2008-06-19 Asuragen, Inc. Functions and targets of let-7 micro RNAs
CN101622349A (en) * 2006-12-08 2010-01-06 奥斯瑞根公司 miR-20 regulated genes and pathways as targets for therapeutic intervention
US20090175827A1 (en) * 2006-12-29 2009-07-09 Byrom Mike W miR-16 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
US8686129B2 (en) * 2007-03-20 2014-04-01 Agilent Technologies, Inc. Methods for the separation of biological molecules using sulfolane
US7964350B1 (en) 2007-05-18 2011-06-21 Applied Biosystems, Llc Sample preparation for in situ nucleic acid analysis
US20090232893A1 (en) * 2007-05-22 2009-09-17 Bader Andreas G miR-143 REGULATED GENES AND PATHWAYS AS TARGETS FOR THERAPEUTIC INTERVENTION
WO2008154333A2 (en) * 2007-06-08 2008-12-18 Asuragen, Inc. Mir-34 regulated genes and pathways as targets for therapeutic intervention
JP5232858B2 (en) * 2007-06-29 2013-07-10 ベクトン・ディキンソン・アンド・カンパニー Method for extracting and purifying components of biological samples
WO2009024574A1 (en) * 2007-08-20 2009-02-26 Novozymes A/S Nuclease reduction
US8361714B2 (en) * 2007-09-14 2013-01-29 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
JP5277497B2 (en) * 2007-09-28 2013-08-28 独立行政法人科学技術振興機構 Purification of RNA extracted from soil
WO2009052386A1 (en) * 2007-10-18 2009-04-23 Asuragen, Inc. Micrornas differentially expressed in lung diseases and uses thereof
EP2217703B1 (en) * 2007-11-30 2017-03-01 GE Healthcare Bio-Sciences Corp. Method for isolation of genomic dna, rna and proteins from a single sample
WO2009070805A2 (en) * 2007-12-01 2009-06-04 Asuragen, Inc. Mir-124 regulated genes and pathways as targets for therapeutic intervention
WO2009086156A2 (en) * 2007-12-21 2009-07-09 Asuragen, Inc. Mir-10 regulated genes and pathways as targets for therapeutic intervention
EP2260110B1 (en) * 2008-02-08 2014-11-12 Asuragen, INC. miRNAs DIFFERENTIALLY EXPRESSED IN LYMPH NODES FROM CANCER PATIENTS
WO2009154835A2 (en) * 2008-03-26 2009-12-23 Asuragen, Inc. Compositions and methods related to mir-16 and therapy of prostate cancer
WO2009126726A1 (en) * 2008-04-08 2009-10-15 Asuragen, Inc Methods and compositions for diagnosing and modulating human papillomavirus (hpv)
US8258111B2 (en) * 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
DE102008032501A1 (en) * 2008-07-10 2010-01-14 Qiagen Gmbh Fast analysis of biological mixed samples
WO2010019898A1 (en) * 2008-08-14 2010-02-18 Zygem Corporation Limited Temperature controlled nucleic-acid detection method suitable for practice in a closed-system
WO2010033652A1 (en) * 2008-09-17 2010-03-25 Ge Healthcare Bio-Sciences Corp. Method for small rna isolation
WO2010056737A2 (en) * 2008-11-11 2010-05-20 Mirna Therapeutics, Inc. Methods and compositions involving mirnas in cancer stem cells
CN102246036B (en) * 2008-12-11 2016-01-20 积水医疗株式会社 The pre-treating method of the sample containing glycosylated hemoglobin
US8211637B2 (en) * 2008-12-19 2012-07-03 Life Technologies Corporation Proteinase K inhibitors, methods and compositions therefor
EP2411539B1 (en) 2009-03-25 2018-10-03 Life Technologies Corporation Discriminatory positive/extraction control dna
EP2264168B1 (en) 2009-06-18 2014-12-17 Qiagen GmbH Method for isolating nucleic acids
US8728752B2 (en) 2009-06-19 2014-05-20 Philipp Bauer Method for isolating Trichinella or other parasites from organic tissue
DE102009025542A1 (en) * 2009-06-19 2011-01-05 Philipp Bauer Detecting intact capsule-forming or non-capsule-forming parasites, e.g. Trichinella papuae, in a meat comprises macerating the meat with a basic digestive solution containing a digestive enzyme, which is active in basic medium
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
JP2013523144A (en) * 2010-04-08 2013-06-17 キアゲン ゲーエムベーハー Method for precipitating anionic surfactant ions in the presence of nucleic acids
WO2011162326A1 (en) * 2010-06-25 2011-12-29 株式会社日本遺伝子研究所 Rna strand storage solution
EP2426201A1 (en) * 2010-09-06 2012-03-07 Qiagen GmbH Method of isolating purified RNA with reduced DNA contaminations
US9273347B2 (en) 2010-09-10 2016-03-01 Bio-Rad Laboratories, Inc. Detection of RNA-interacting regions in DNA
US8652821B2 (en) 2010-10-20 2014-02-18 Bio-Rad Laboratories, Inc. Stabilized protease-containing solutions for purifying RNA-free DNA
CN103370412B (en) * 2010-12-04 2015-07-22 万芬 RNA stability enhancer
WO2012106586A1 (en) 2011-02-03 2012-08-09 Mirna Therapeutics, Inc. Synthetic mimics of mir-124
WO2012112606A1 (en) 2011-02-15 2012-08-23 Bio-Rad Laboratories, Inc. Detecting methylati0n in a subpopulation of genomic dna
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
EP3928715A1 (en) 2011-06-19 2021-12-29 DNA Genotek, Inc. Devices, solutions and methods for sample collection
WO2013019960A1 (en) 2011-08-03 2013-02-07 Bio-Rad Laboratories, Inc. Filtering small nucleic acids using permeabilized cells
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
CN104080958A (en) 2011-10-19 2014-10-01 纽亘技术公司 Compositions and methods for directional nucleic acid amplification and sequencing
GB2513793B (en) 2012-01-26 2016-11-02 Nugen Tech Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
EP2861787B1 (en) 2012-06-18 2017-09-20 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
CN104812947B (en) 2012-07-17 2018-04-27 考希尔股份有限公司 The system and method for detecting hereditary variation
US9422602B2 (en) 2012-08-15 2016-08-23 Bio-Rad Laboratories, Inc. Methods and compositions for determining nucleic acid degradation
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
GB2519255B (en) 2013-02-01 2016-01-06 Univ California Methods for genome assembly and haplotype phasing
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
SG11201600550WA (en) 2013-07-25 2016-02-26 Dch Molecular Diagnostics Inc Methods and compositions for detecting bacterial contamination
US9534214B2 (en) 2013-10-31 2017-01-03 General Electric Company Substrates and associated methods for elution of nucleic acids
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
WO2015089243A1 (en) 2013-12-11 2015-06-18 The Regents For Of The University Of California Methods for labeling dna fragments to recontruct physical linkage and phase
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
AU2014362227B2 (en) 2013-12-11 2021-05-13 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
EP3653723B1 (en) 2014-02-10 2022-07-20 Zymo Research Corporation Methods for nucleic acid capture
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US11060149B2 (en) 2014-06-18 2021-07-13 Clear Gene, Inc. Methods, compositions, and devices for rapid analysis of biological markers
US20160017316A1 (en) * 2014-07-18 2016-01-21 Bionano Genomics, Inc. Isolation of megabase-sized dna from plant and animal tissues
WO2016019360A1 (en) 2014-08-01 2016-02-04 Dovetail Genomics Llc Tagging nucleic acids for sequence assembly
CN107109474B (en) 2014-08-19 2021-03-19 豪夫迈·罗氏有限公司 Methods and compositions for nucleic acid detection
EP3259696A1 (en) 2015-02-17 2017-12-27 Dovetail Genomics LLC Nucleic acid sequence assembly
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
EP3328999B1 (en) 2015-07-30 2020-03-25 Arcis Biotechnology Holdings Limited Method using a quaternary ammonium compound
EP4289356A3 (en) 2015-09-09 2024-02-28 Drawbridge Health, Inc. Devices for sample collection, stabilization and preservation
AU2016334233B2 (en) 2015-10-09 2023-01-05 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN108368542B (en) 2015-10-19 2022-04-08 多弗泰尔基因组学有限责任公司 Methods for genome assembly, haplotype phasing, and target-independent nucleic acid detection
AU2016369603A1 (en) 2015-12-18 2018-07-05 Clear Gene, Inc. Methods, compositions, kits and devices for rapid analysis of biological markers
JP7441003B2 (en) 2016-02-23 2024-02-29 ダブテイル ゲノミクス エルエルシー Generation of phased read sets and haplotype phasing for genome assembly
EP3954771A1 (en) 2016-05-13 2022-02-16 Dovetail Genomics, LLC Recovering long-range linkage information from preserved samples
US11427866B2 (en) 2016-05-16 2022-08-30 Accuragen Holdings Limited Method of improved sequencing by strand identification
WO2017218777A1 (en) 2016-06-17 2017-12-21 California Institute Of Technology Nucleic acid reactions and related methods and compositions
WO2018005811A1 (en) 2016-06-30 2018-01-04 Grail, Inc. Differential tagging of rna for preparation of a cell-free dna/rna sequencing library
JP6966052B2 (en) 2016-08-15 2021-11-10 アキュラーゲン ホールディングス リミテッド Compositions and Methods for Detecting Rare Sequence Variants
CA3042298A1 (en) 2016-11-08 2018-05-17 Qvella Corporation Methods of performing nucleic acid stabilization and separation
EP3595536A4 (en) 2017-03-15 2020-12-23 Ancestry.com DNA, LLC Sample collection device and method
EP3612646A1 (en) 2017-04-18 2020-02-26 Dovetail Genomics, LLC Nucleic acid characteristics as guides for sequence assembly
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
WO2018232126A1 (en) * 2017-06-14 2018-12-20 Fabrico Technology Inc. Decellularized and demineralized bone matrices and methods for making same
US10973775B2 (en) * 2017-09-22 2021-04-13 University Of Manitoba Antibacterial nanofiber
MX2020003624A (en) 2017-10-06 2020-09-25 Ancestry Com Dna Llc Systems, devices, and methods for sample collection.
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
JP2021504728A (en) 2017-11-22 2021-02-15 アンセストリー ドットコム ディーエヌエー リミテッド ライアビリティ カンパニー Sampling kit with cap with selectively movable sleeve
US11426734B2 (en) 2017-11-22 2022-08-30 Ancestry.Com Dna, Llc Sample collection kit including cap having selectively movable sleeve
US11685915B2 (en) 2017-12-27 2023-06-27 Toray Industries, Inc. Method of collecting nucleic acid
CA3090102A1 (en) 2018-01-31 2019-08-08 Dovetail Genomics, Llc Sample prep for dna linkage recovery
US11203782B2 (en) 2018-03-29 2021-12-21 Accuragen Holdings Limited Compositions and methods comprising asymmetric barcoding
CA3131344A1 (en) 2019-02-27 2020-09-03 Ancestry.Com Dna, Llc Graphical user interface displaying relatedness based on shared dna
DE102019108087A1 (en) * 2019-03-28 2020-10-01 Axagarius Gmbh & Co. Kg Method for the isolation of nucleic acids from sample materials
CN112176029B (en) 2020-06-12 2021-05-14 中山大学达安基因股份有限公司 Swab nucleic acid sample releasing agent and application thereof
WO2022197637A1 (en) * 2021-03-15 2022-09-22 Gen-Probe Incorporated Compositions and methods for biological sample processing

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015970A1 (en) * 1993-12-06 1995-06-15 Molecular Tool, Inc. Method for the immobilization of nucleic acid molecules
WO1998004730A1 (en) * 1996-07-29 1998-02-05 Promega Corporation Methods for isolating nucleic acids using alkaline protease
EP1018549A1 (en) * 1997-09-22 2000-07-12 The Institute Of Physical & Chemical Research Method for isolating dna
DE19937607A1 (en) * 1999-08-09 2001-02-15 Bilatec Ges Zur Entwicklung Bi Laboratory robot, useful for automated isolation of nucleic acid, includes orientation device to ensure proper alignment between robotic arm and unit being transported

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3269855D1 (en) 1981-11-03 1986-04-17 Shell Int Research Process for cell disruption
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
DE3704465C2 (en) * 1987-02-13 1995-11-02 Roehm Gmbh Liquid formulations of enzymes
US5010183A (en) 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
IT1240870B (en) 1990-02-14 1993-12-17 Talent PROCEDURE FOR THE EXTRACTION AND PURIFICATION OF HUMAN GENOMIC DNA
US5130423A (en) * 1990-07-13 1992-07-14 Microprobe Corporation Non-corrosive compositions and methods useful for the extraction of nucleic acids
JP3018802B2 (en) 1992-12-04 2000-03-13 和光純薬工業株式会社 DNA extraction method and extraction kit from whole blood sample
WO1994018156A1 (en) 1993-02-01 1994-08-18 University Of Iowa Research Foundation Quartenary amine surfactants and methods of using same in isolation of rna
US5300635A (en) 1993-02-01 1994-04-05 University Of Iowa Research Foundation Quaternary amine surfactants and methods of using same in isolation of nucleic acids
JPH07143879A (en) 1993-11-24 1995-06-06 Toagosei Co Ltd Separation and purification of dna
WO1998020164A1 (en) 1996-11-04 1998-05-14 Cornell Research Foundation, Inc. Matrix mill for dna extraction
CA2270023C (en) 1998-04-28 2010-10-12 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
DE19916534A1 (en) 1999-04-13 2000-10-19 Gsf Forschungszentrum Umwelt RNA isolation kit
US6242188B1 (en) * 1999-07-30 2001-06-05 Applied Gene Technologies, Inc. Sample processing to release nucleic acids for direct detection
AU6281200A (en) * 1999-08-09 2001-03-05 Bilatec Ag Laboratory robot and method and reagent kit for isolating nucleic acids
US6548256B2 (en) 2000-07-14 2003-04-15 Eppendorf 5 Prime, Inc. DNA isolation method and kit
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995015970A1 (en) * 1993-12-06 1995-06-15 Molecular Tool, Inc. Method for the immobilization of nucleic acid molecules
WO1998004730A1 (en) * 1996-07-29 1998-02-05 Promega Corporation Methods for isolating nucleic acids using alkaline protease
EP1018549A1 (en) * 1997-09-22 2000-07-12 The Institute Of Physical & Chemical Research Method for isolating dna
DE19937607A1 (en) * 1999-08-09 2001-02-15 Bilatec Ges Zur Entwicklung Bi Laboratory robot, useful for automated isolation of nucleic acid, includes orientation device to ensure proper alignment between robotic arm and unit being transported

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US7303876B2 (en) 2007-12-04
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US6762027B2 (en) 2004-07-13
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