WO2002101358A3 - Multiplexed detection methods - Google Patents

Multiplexed detection methods Download PDF

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Publication number
WO2002101358A3
WO2002101358A3 PCT/US2002/018634 US0218634W WO02101358A3 WO 2002101358 A3 WO2002101358 A3 WO 2002101358A3 US 0218634 W US0218634 W US 0218634W WO 02101358 A3 WO02101358 A3 WO 02101358A3
Authority
WO
WIPO (PCT)
Prior art keywords
target
probes
multiplexed detection
method includes
target molecules
Prior art date
Application number
PCT/US2002/018634
Other languages
French (fr)
Other versions
WO2002101358A9 (en
WO2002101358A2 (en
Inventor
Jian-Bing Fan
John R Stuelpnagel
Mark S Chee
Original Assignee
Illumina Inc
Jian-Bing Fan
John R Stuelpnagel
Mark S Chee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/915,231 external-priority patent/US6890741B2/en
Application filed by Illumina Inc, Jian-Bing Fan, John R Stuelpnagel, Mark S Chee filed Critical Illumina Inc
Priority to CA002450139A priority Critical patent/CA2450139A1/en
Priority to EP02744306A priority patent/EP1395805A4/en
Publication of WO2002101358A2 publication Critical patent/WO2002101358A2/en
Publication of WO2002101358A9 publication Critical patent/WO2002101358A9/en
Publication of WO2002101358A3 publication Critical patent/WO2002101358A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

Abstract

The present invention permits highly multiplexed detection of target molecules in a sample. The method includes contacting target analytes with a composition comprising an amplification enzyme and first and second target probes. The first and second target probes comprise first and second bioactive agent respectively, that specifically bind to the first and second target molecules. The probes may also comprise first and second adapter sequences, respectively, such and that the first adapter sequence identifies the first target molecule and the second adapter sequence identifies the second target molecule, and first and section upstream universal priming sequences, respectively. Finally, following amplification the method includes detecting the first and second amplicons, whereby the first and second target molecules, respectively are detected.
PCT/US2002/018634 2001-06-11 2002-06-11 Multiplexed detection methods WO2002101358A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CA002450139A CA2450139A1 (en) 2001-06-11 2002-06-11 Multiplexed detection methods
EP02744306A EP1395805A4 (en) 2001-06-11 2002-06-11 Multiplexed detection methods

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US29760901P 2001-06-11 2001-06-11
US60/297,609 2001-06-11
US09/915,231 US6890741B2 (en) 2000-02-07 2001-07-24 Multiplexed detection of analytes
US09/915,231 2001-07-24
US31127101P 2001-08-09 2001-08-09
US60/311,271 2001-08-09

Publications (3)

Publication Number Publication Date
WO2002101358A2 WO2002101358A2 (en) 2002-12-19
WO2002101358A9 WO2002101358A9 (en) 2003-03-27
WO2002101358A3 true WO2002101358A3 (en) 2003-12-11

Family

ID=27404500

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/018634 WO2002101358A2 (en) 2001-06-11 2002-06-11 Multiplexed detection methods

Country Status (3)

Country Link
EP (1) EP1395805A4 (en)
CA (1) CA2450139A1 (en)
WO (1) WO2002101358A2 (en)

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WO1999067641A2 (en) 1998-06-24 1999-12-29 Illumina, Inc. Decoding of array sensors with microspheres
US7241629B2 (en) 2001-12-20 2007-07-10 Corning Incorporated Detectable labels, methods of manufacture and use
JP5171037B2 (en) 2003-09-10 2013-03-27 アルセア・ディーエックス，インク． Expression profiling using microarrays
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
KR102536833B1 (en) 2013-08-28 2023-05-26 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
EP3262192B1 (en) 2015-02-27 2020-09-16 Becton, Dickinson and Company Spatially addressable molecular barcoding
EP4180535A1 (en) 2015-03-30 2023-05-17 Becton, Dickinson and Company Methods and compositions for combinatorial barcoding
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
CN108026524A (en) 2015-09-11 2018-05-11 赛卢拉研究公司 Method and composition for nucleic acid library standardization
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP4050112A1 (en) 2016-06-21 2022-08-31 10X Genomics, Inc. Nucleic acid sequencing
JP7091348B2 (en) 2016-09-26 2022-06-27 ベクトン・ディキンソン・アンド・カンパニー Measurement of protein expression using reagents with barcoded oligonucleotide sequences
CN110382708A (en) 2017-02-01 2019-10-25 赛卢拉研究公司 Selective amplification is carried out using blocking property oligonucleotides
CN110719959B (en) 2017-06-05 2021-08-06 贝克顿迪金森公司 Sample indexing for single cells
WO2019213237A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company Molecular barcoding on opposite transcript ends
WO2019213294A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
JP2022511398A (en) 2018-10-01 2022-01-31 ベクトン・ディキンソン・アンド・カンパニー Determining the 5'transcription sequence
US11104888B2 (en) 2018-10-31 2021-08-31 Illumina, Inc. Polymerases, compositions, and methods of use
JP2022506546A (en) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー Single-cell whole transcriptome analysis using random priming
WO2020117968A2 (en) 2018-12-05 2020-06-11 Illumina, Inc. Polymerases, compositions, and methods of use
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
EP4242322A3 (en) 2019-01-23 2023-09-20 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins

Citations (2)

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US6027889A (en) * 1996-05-29 2000-02-22 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays

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US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
AT407160B (en) * 1998-06-04 2001-01-25 Immuno Ag METHOD FOR DETERMINING ANTIGENS
EP1165839A2 (en) * 1999-03-26 2002-01-02 Whitehead Institute For Biomedical Research Universal arrays
ATE553219T1 (en) * 1999-04-20 2012-04-15 Illumina Inc DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS
WO2001006012A1 (en) * 1999-07-14 2001-01-25 Packard Bioscience Company Derivative nucleic acids and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027889A (en) * 1996-05-29 2000-02-22 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6355431B1 (en) * 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1395805A4 *

Also Published As

Publication number Publication date
WO2002101358A9 (en) 2003-03-27
EP1395805A4 (en) 2005-03-09
CA2450139A1 (en) 2002-12-19
EP1395805A2 (en) 2004-03-10
WO2002101358A2 (en) 2002-12-19

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