WO2002102837A3 - Marker for identifying hematopoietic stem cells - Google Patents

Marker for identifying hematopoietic stem cells Download PDF

Info

Publication number
WO2002102837A3
WO2002102837A3 PCT/EP2002/005918 EP0205918W WO02102837A3 WO 2002102837 A3 WO2002102837 A3 WO 2002102837A3 EP 0205918 W EP0205918 W EP 0205918W WO 02102837 A3 WO02102837 A3 WO 02102837A3
Authority
WO
WIPO (PCT)
Prior art keywords
stem cells
hematopoietic
hematopoietic stem
hhpclp
pclps
Prior art date
Application number
PCT/EP2002/005918
Other languages
French (fr)
Other versions
WO2002102837A2 (en
Inventor
Aaro Miettinen
Laura Kerosuo
Dontscho Kerjaschki
Original Assignee
Innovationsagentur Gmbh
Aaro Miettinen
Laura Kerosuo
Dontscho Kerjaschki
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innovationsagentur Gmbh, Aaro Miettinen, Laura Kerosuo, Dontscho Kerjaschki filed Critical Innovationsagentur Gmbh
Priority to AU2002328803A priority Critical patent/AU2002328803A1/en
Publication of WO2002102837A2 publication Critical patent/WO2002102837A2/en
Publication of WO2002102837A3 publication Critical patent/WO2002102837A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells

Abstract

The present invention is related to a method for identifying, isolating and manufacturing pluripotent hematopoietic stem useful as transplants for treating blood diseases. The hematopoietic stem cells and/or committed progenitor cells are identified using antibodies capable of specifically recognizing human podocalyxin- like proteins (hPCLPs) expressed in hematopoietic tissues. The : human hematopoietic tissue derived hhPCLPs are useful as markers for assessing the quality of stem cells obtained by conventional methods and which comprise only a fraction of undifferentiated pluripotent stem cells. The hhPCLP differs from other known PCLPs in that it is obtainable from the hematopoietic tissue, has a specific glycosylation pattern and in its glycosylated form an apparent MW of lOO-llO kDa. Said hhPCLP can be identified with antibodies raised against PCLPs from different species and tis- sues by per se known methods.
PCT/EP2002/005918 2001-05-30 2002-05-29 Marker for identifying hematopoietic stem cells WO2002102837A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002328803A AU2002328803A1 (en) 2001-05-30 2002-05-29 Marker for identifying hematopoietic stem cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI20011132A FI20011132A0 (en) 2001-05-30 2001-05-30 A marker for the identification of hematopoietic stem cells
FI20011132 2001-05-30

Publications (2)

Publication Number Publication Date
WO2002102837A2 WO2002102837A2 (en) 2002-12-27
WO2002102837A3 true WO2002102837A3 (en) 2003-08-28

Family

ID=8561301

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/005918 WO2002102837A2 (en) 2001-05-30 2002-05-29 Marker for identifying hematopoietic stem cells

Country Status (3)

Country Link
AU (1) AU2002328803A1 (en)
FI (1) FI20011132A0 (en)
WO (1) WO2002102837A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9364565B2 (en) 2000-03-15 2016-06-14 Orbusneich Medical, Inc. Medical device with coating for capturing genetically-altered cells and methods of using same
US9522217B2 (en) 2000-03-15 2016-12-20 Orbusneich Medical, Inc. Medical device with coating for capturing genetically-altered cells and methods for using same

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4493882B2 (en) 2001-06-19 2010-06-30 株式会社カネカ Antigen and monoclonal antibody that distinguishes this antigen
GB0228409D0 (en) 2002-12-06 2003-01-08 Thromb X Nv Pharmacological vitreolysis
GB2434867B (en) * 2003-03-13 2007-11-07 Geron Corp Genes that are up-or down-regulated during differentiation of human embryonic stem cells
US7153650B2 (en) * 2003-03-13 2006-12-26 Geron Corporation Marker system for preparing and characterizing high-quality human embryonic stem cells
JPWO2005054459A1 (en) * 2003-12-04 2007-06-28 株式会社東京大学Ｔｌｏ Method for producing hematopoietic stem cells or vascular endothelial progenitor cells
CN100464189C (en) * 2006-04-21 2009-02-25 深圳市新产业生物医学工程有限公司 Magnetic separating direct chemical illuminating reagent and testing method using the same reagent
EP2315028A1 (en) * 2009-10-26 2011-04-27 Atlas Antibodies AB PODXL protein in colorectal cancer
CN103055418A (en) * 2011-10-24 2013-04-24 北京清美联创干细胞科技有限公司 Fast stem cell homing method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827742A (en) * 1994-09-01 1998-10-27 Beth Israel Deaconess Medical Center, Inc. Method of selecting pluripotent hematopioetic progenitor cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5827742A (en) * 1994-09-01 1998-10-27 Beth Israel Deaconess Medical Center, Inc. Method of selecting pluripotent hematopioetic progenitor cells

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CLAUSEN P A ET AL: "ETS-1 INDUCES INCREASED EXPRESSION OF ERYTHROID MARKERS IN THE PLURIPOTENT ERYTHROLEUKEMIC CELL LINE K562 AND HEL", LEUKEMIA, MACMILLAN PRESS LTD, US, vol. 11, 1997, pages 1224 - 1233, XP002927961, ISSN: 0887-6924 *
HARA T ET AL: "IDENTIFICATION OF PODOCALYXIN-LIKE PROTEIN 1 AS A NOVEL CELL SURFACE MARKER FOR HEMANGIOBLASTS IN THE MURINE AORTA-GONAD-MESONEPHROS REGION", IMMUNITY, CELL PRESS, US, vol. 11, no. 5, November 1999 (1999-11-01), pages 567 - 578, XP002937028, ISSN: 1074-7613 *
KERSHAW DAVID B ET AL: "Molecular cloning and characterization of human podocalyxin-like protein. Orthologous relationship to rabbit PCLP1 and rat podocalyxin.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 25, 1997, pages 15708 - 15714, XP002232096, ISSN: 0021-9258 *
MCNAGNY KELLY M ET AL: "Thrombomucin, a novel cell surface protein that defines thrombocytes and multipotent hematopoietic progenitors.", JOURNAL OF CELL BIOLOGY, vol. 138, no. 6, 1997, pages 1395 - 1407, XP002232095, ISSN: 0021-9525 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9364565B2 (en) 2000-03-15 2016-06-14 Orbusneich Medical, Inc. Medical device with coating for capturing genetically-altered cells and methods of using same
US9522217B2 (en) 2000-03-15 2016-12-20 Orbusneich Medical, Inc. Medical device with coating for capturing genetically-altered cells and methods for using same

Also Published As

Publication number Publication date
AU2002328803A1 (en) 2003-01-02
FI20011132A0 (en) 2001-05-30
WO2002102837A2 (en) 2002-12-27

Similar Documents

Publication Publication Date Title
WO2006044204A3 (en) Neuronal progenitors from feeder-free human embryonic stem cell culture
EP2446892A3 (en) Isolated adult pluripotent stem cells and method for isolating and cultivating same
WO2002102837A3 (en) Marker for identifying hematopoietic stem cells
WO2006092668A3 (en) Fetal skin cell protein compositions for the treatment of skin conditions, disorders or diseases and methods of making and using the same
WO2003060085A3 (en) Mammalian neural stem cells, compositions and uses thereof
WO2003085099A3 (en) A process for the preparation of stem cells from human muscle tissue and adipose tissue, and stem cells obtainable by this process
WO2002064748A3 (en) Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof
WO2001096580A3 (en) Method for modifying plant morphology, biochemistry and physiology using cytokinin oxidases
WO2007002261A3 (en) IL-1β BINDING ANTIBODIES AND FRAGMENTS THEREOF
EP3275901A8 (en) Human embryonic stem cell methods and podxl expression
WO2006029347A3 (en) Integrin cd18 is a novel stromal stem cell marker and functions to promote osteogenesis
EA200601187A1 (en) STEM CELLS
WO2007012695A3 (en) Tissue carbohydrate compositions and analysis thereof
WO2007062387A3 (en) Hair grafts derived from plucked hair
WO2003024472A3 (en) Prolactin induced increase in neutral stem cell numbers and therapeutical use thereof
WO2006093860A3 (en) Method and composition for repairing heart tissue
ATE455853T1 (en) ANTIBODIES AGAINST DAMAGED TISSUE
WO2007058982A3 (en) Mntf differentiation and growth of stem cells
WO2010082177A3 (en) Compounds for cosmetic and dermopharmaceutical
WO2004009758A3 (en) Embryonic stem cell markers and uses thereof
WO2005000335A3 (en) Use of genes differentially expressed during aging of liver for treatment and diagnosis
WO2005056755A3 (en) Compositions and methods for propagation of neural progenitor cells
WO2010087771A8 (en) Compound and method for treatment of alzheimer's disease
WO2003008449A8 (en) Ntb-a, a surface molecule involved in natural killer cells activity
Wojtaszek et al. Changes in the composition of exocellular proteins of suspension-cultured Lupinus albus cells in response to fungal elicitors or CuCl2

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

122 Ep: pct application non-entry in european phase
NENP Non-entry into the national phase

Ref country code: JP

WWW Wipo information: withdrawn in national office

Country of ref document: JP