WO2003004992A3 - Mammalian sweet and amino acid heterodimeric taste receptors - Google Patents

Mammalian sweet and amino acid heterodimeric taste receptors Download PDF

Info

Publication number
WO2003004992A3
WO2003004992A3 PCT/US2002/021269 US0221269W WO03004992A3 WO 2003004992 A3 WO2003004992 A3 WO 2003004992A3 US 0221269 W US0221269 W US 0221269W WO 03004992 A3 WO03004992 A3 WO 03004992A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
receptors
taste receptors
sweet
heterodimeric taste
Prior art date
Application number
PCT/US2002/021269
Other languages
French (fr)
Other versions
WO2003004992A2 (en
Inventor
Charles S Zuker
Nicholas J P Ryba
Jayaram Chandrashekar
Mark A Hoon
Gregory A Nelson
Yifeng Zhang
Original Assignee
Univ California
Us Gov Health & Human Serv
Charles S Zuker
Nicholas J P Ryba
Jayaram Chandrashekar
Mark A Hoon
Gregory A Nelson
Yifeng Zhang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/927,315 external-priority patent/US7402400B2/en
Application filed by Univ California, Us Gov Health & Human Serv, Charles S Zuker, Nicholas J P Ryba, Jayaram Chandrashekar, Mark A Hoon, Gregory A Nelson, Yifeng Zhang filed Critical Univ California
Priority to EP02749804.7A priority Critical patent/EP1585954B1/en
Priority to JP2003510918A priority patent/JP2005505262A/en
Priority to CA2452716A priority patent/CA2452716C/en
Priority to AU2002320294A priority patent/AU2002320294B2/en
Publication of WO2003004992A2 publication Critical patent/WO2003004992A2/en
Publication of WO2003004992A3 publication Critical patent/WO2003004992A3/en
Priority to AU2009206153A priority patent/AU2009206153B2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5076—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving cell organelles, e.g. Golgi complex, endoplasmic reticulum

Abstract

The present invention provides isolated nucleic acid and amino acid sequences of sweet or amino acid taste receptors comprising two heterologous G-protein coupled receptor polypeptides from the T1R family of sensory G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of sweet and amino acid taste receptors.
PCT/US2002/021269 2001-07-03 2002-07-03 Mammalian sweet and amino acid heterodimeric taste receptors WO2003004992A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP02749804.7A EP1585954B1 (en) 2001-07-03 2002-07-03 Mammalian sweet and amino acid heterodimeric taste receptors
JP2003510918A JP2005505262A (en) 2001-07-03 2002-07-03 Mammalian sweet taste and amino acid heterodimeric taste receptors
CA2452716A CA2452716C (en) 2001-07-03 2002-07-03 Mammalian sweet and amino acid heterodimeric taste receptors
AU2002320294A AU2002320294B2 (en) 2001-07-03 2002-07-03 Mammalian sweet and amino acid heterodimeric taste receptors
AU2009206153A AU2009206153B2 (en) 2001-07-03 2009-08-04 Mammalian sweet and amino acid heterodimeric taste receptors

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US30289801P 2001-07-03 2001-07-03
US60/302,898 2001-07-03
US09/927,315 2001-08-10
US09/927,315 US7402400B2 (en) 2001-07-03 2001-08-10 Mammalian sweet taste receptors
US35892502P 2002-02-22 2002-02-22
US60/358,925 2002-02-22

Publications (2)

Publication Number Publication Date
WO2003004992A2 WO2003004992A2 (en) 2003-01-16
WO2003004992A3 true WO2003004992A3 (en) 2006-10-05

Family

ID=27404902

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/021269 WO2003004992A2 (en) 2001-07-03 2002-07-03 Mammalian sweet and amino acid heterodimeric taste receptors

Country Status (6)

Country Link
US (4) US7588900B2 (en)
EP (1) EP1585954B1 (en)
JP (3) JP2005505262A (en)
AU (2) AU2002320294B2 (en)
CA (1) CA2452716C (en)
WO (1) WO2003004992A2 (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8895050B2 (en) 2003-08-06 2014-11-25 Senomyx, Inc. Flavors, flavor modifiers, tastants, taste enhancers, umami or sweet tastants, and/or enhancers and use thereof
US9176130B2 (en) 2001-03-07 2015-11-03 Senomyx, Inc. Methods of screening T1R1/T1R3 receptors for compounds that modulate umami taste signaling
US9476869B2 (en) 2001-04-19 2016-10-25 Senomyx, Inc. Methods of screening T1R hetero-oligomeric taste receptors for sweet taste modulators
US9481722B2 (en) 2001-01-03 2016-11-01 Senomyx, Inc. T1R taste receptors and genes encoding same

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7402400B2 (en) 2001-07-03 2008-07-22 Regents Of The University Of California Mammalian sweet taste receptors
DK1100811T3 (en) 1998-07-28 2007-11-05 Univ California Nucleic acids that encode a G protein-coupled receptor involved in sensory transduction
US7588900B2 (en) * 2001-07-03 2009-09-15 The Regents Of The University Of California Mammalian sweet and amino acid heterodimeric taste receptors
TW201006846A (en) 2000-03-07 2010-02-16 Senomyx Inc T1R taste receptor and genes encidung same
US7364867B2 (en) 2000-04-17 2008-04-29 The Mount Sinai School Of Medicine Method of identifying bitter compounds by employing TRP8, a transient receptor potential channel expressed in taste receptor cells
US20080244761A1 (en) * 2001-03-07 2008-10-02 Senomyx, Inc. T1r hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
US7763431B1 (en) 2001-03-07 2010-07-27 Senomyx, Inc. Binding assays that use the T1R2/T1R3 (sweet) taste receptor to identify compounds that elicit or modulate sweet taste
US7301009B2 (en) * 2001-06-26 2007-11-27 Senomyx, Inc. Isolated (T1R1/T1R3) umami taste receptors that respond to umami taste stimuli
US7309577B2 (en) * 2001-03-07 2007-12-18 Senomyx, Inc. Binding assays that use the T1R1/T1R3 (umami) taste receptor to identify compounds that elicit or modulate umami taste
US20040219632A1 (en) * 2001-04-20 2004-11-04 Robert Margolskee T1r3 a novel taste receptor
US7803982B2 (en) 2001-04-20 2010-09-28 The Mount Sinai School Of Medicine Of New York University T1R3 transgenic animals, cells and related methods
US7335487B2 (en) 2001-09-18 2008-02-26 Irm Llc Sweet taste receptors
AU2003230176A1 (en) * 2002-05-20 2003-12-19 Tvd Taste Virtual Dimensions, Inc. Antibodies and their use for the modulation of taste sensation___
WO2006113422A2 (en) * 2005-04-13 2006-10-26 The Government Of The Usa As Represented By The Secretary Of The Dept. Of Health And Human Services Human sweet and umami taste receptor variants
DK3235811T3 (en) 2006-04-21 2018-11-12 Senomyx Inc PROCEDURE FOR THE PREPARATION OF OXALAMIDS
JP2009539377A (en) * 2006-06-08 2009-11-19 セノミックス インコーポレイテッド Rationale, methods, and assays for identifying novel taste cell genes and salty taste receptor targets and assays using these identified genes or gene products
WO2007147275A1 (en) 2006-06-19 2007-12-27 Givaudan Sa Nucleic acid, polypeptide and its use
JP5926475B2 (en) * 2006-09-21 2016-05-25 ユニバーシティー オブ ロチェスター Compositions and methods for protein replacement therapy for myotonic dystrophy
JP5301464B2 (en) * 2007-01-18 2013-09-25 ジボダン エス エー Chimeric umami receptor, nucleic acid encoding it, and use thereof
US9603848B2 (en) 2007-06-08 2017-03-28 Senomyx, Inc. Modulation of chemosensory receptors and ligands associated therewith
US7928111B2 (en) * 2007-06-08 2011-04-19 Senomyx, Inc. Compounds including substituted thienopyrimidinone derivatives as ligands for modulating chemosensory receptors
WO2009100040A2 (en) * 2008-02-01 2009-08-13 Chromocell Corporation Cell lines expressing gabaa and methods using them
EP2682460B1 (en) 2008-07-07 2017-04-26 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CN102144037A (en) 2008-07-07 2011-08-03 牛津纳米孔技术有限公司 Base-detecting pore
US8586733B2 (en) 2008-07-31 2013-11-19 Senomyx, Inc. Processes and intermediates for making sweet taste enhancers
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
JP5843614B2 (en) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド Adapters for nucleic acid constructs in transmembrane sequencing
WO2010086603A1 (en) * 2009-01-30 2010-08-05 Oxford Nanopore Technologies Limited Enzyme mutant
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
CN103460040B (en) 2011-02-11 2016-08-17 牛津纳米孔技术有限公司 Saltant type hole
EP2529632B1 (en) 2011-05-31 2013-08-28 Symrise AG Cinnamic acid amides as spicy flavour agents
CN103827320B (en) 2011-07-25 2017-04-26 牛津纳米孔技术有限公司 Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
EP2742350B1 (en) 2011-08-08 2019-10-30 The Coca-Cola Company Cell lines comprising endogenous taste receptors and their uses
US20130149351A1 (en) * 2011-12-09 2013-06-13 University Of Southern California Polymer scaffolds and their use in the treatment of vision loss
US9777049B2 (en) 2012-04-10 2017-10-03 Oxford Nanopore Technologies Ltd. Mutant lysenin pores
WO2014013259A1 (en) 2012-07-19 2014-01-23 Oxford Nanopore Technologies Limited Ssb method
BR112015002380B1 (en) 2012-08-06 2021-09-28 Firmenich Incorporated COMPOUND, INGERIBLE COMPOSITIONS, PROCESSES TO INCREASE THE SWEET FLAVOR OF COMPOSITION AND CONCENTRATED FLAVORIZING FORMULATION
JO3155B1 (en) 2013-02-19 2017-09-20 Senomyx Inc Sweet flavor modifier
CN105209634B (en) 2013-03-08 2020-05-12 牛津纳米孔技术公司 Enzyme arrest method
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US9804157B1 (en) 2013-03-15 2017-10-31 Senomyx, Inc. Screening assays to identify compounds which modulate T1R associated taste modalities which eliminate false positives
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
US10908147B2 (en) 2013-12-16 2021-02-02 Monell Chemical Senses Center Methods of identifying modulators of olfactory receptors
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
EP2918270A1 (en) 2014-03-12 2015-09-16 Symrise AG Derivatives of aromatic alkenoic acids for curbing appetite and enhancing mood
EP3137627A1 (en) 2014-05-02 2017-03-08 Oxford Nanopore Technologies Limited Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
KR20170042794A (en) 2014-09-01 2017-04-19 브이아이비 브이지더블유 Mutant csgg pores
WO2016055778A1 (en) 2014-10-07 2016-04-14 Oxford Nanopore Technologies Limited Mutant pores
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
EP3814344A1 (en) 2018-08-07 2021-05-05 Firmenich Incorporated 5-substituted 4-amino-1h-benzo[c][1,2,6]thiadiazine 2,2-dioxides and formulations and uses thereof
WO2023238955A1 (en) * 2022-06-09 2023-12-14 株式会社 Ion Chat Research Method for controlling membrane potential-dependent ion channel through type i taste receptors (t1rs)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000006593A1 (en) * 1998-07-28 2000-02-10 The Regents Of The University Of California Nucleic acids encoding a g-protein coupled receptor involved in sensory transduction
US6955887B2 (en) * 2001-03-30 2005-10-18 Senomyx, Inc. Use of T1R hetero-oligomeric taste receptor to screen for compounds that modulate taste signaling

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7402400B2 (en) * 2001-07-03 2008-07-22 Regents Of The University Of California Mammalian sweet taste receptors
US7588900B2 (en) * 2001-07-03 2009-09-15 The Regents Of The University Of California Mammalian sweet and amino acid heterodimeric taste receptors
WO2001064882A2 (en) 2000-02-29 2001-09-07 Millennium Pharmaceuticals, Inc. 1983, 52881, 2398, 45449, 50289, and 52872, g protein-coupled receptors and uses therefor
TW201006846A (en) 2000-03-07 2010-02-16 Senomyx Inc T1R taste receptor and genes encidung same
WO2001083749A2 (en) * 2000-04-28 2001-11-08 Warner-Lambert Company Gene and sequence variation associated with sensing carbohydrate compounds and other sweeteners
TW201022287A (en) * 2001-01-03 2010-06-16 Senomyx Inc T1R taste receptors and genes encoding same
US7368285B2 (en) * 2001-03-07 2008-05-06 Senomyx, Inc. Heteromeric umami T1R1/T1R3 taste receptors and isolated cells that express same
US20040219632A1 (en) * 2001-04-20 2004-11-04 Robert Margolskee T1r3 a novel taste receptor
JP3698069B2 (en) 2001-05-01 2005-09-21 独立行政法人食品総合研究所 New gene
US20030143668A1 (en) 2001-06-18 2003-07-31 National Institute Of Advanced Industrial Guanosine triphosphate-binding protein coupled receptors
CN101891821A (en) * 2001-06-26 2010-11-24 塞诺米克斯公司 T1R hetero-oligomeric taste receptor, and cell line expressing the receptor, and use thereof for identification of tasty compound
AU2002326315B2 (en) * 2001-06-26 2007-07-05 Senomyx, Inc. T1R hetero-oligomeric taste receptors and cell lines that express said receptors and use thereof for identification of taste compounds
US7335487B2 (en) 2001-09-18 2008-02-26 Irm Llc Sweet taste receptors

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000006593A1 (en) * 1998-07-28 2000-02-10 The Regents Of The University Of California Nucleic acids encoding a g-protein coupled receptor involved in sensory transduction
US6955887B2 (en) * 2001-03-30 2005-10-18 Senomyx, Inc. Use of T1R hetero-oligomeric taste receptor to screen for compounds that modulate taste signaling

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9481722B2 (en) 2001-01-03 2016-11-01 Senomyx, Inc. T1R taste receptors and genes encoding same
US9176130B2 (en) 2001-03-07 2015-11-03 Senomyx, Inc. Methods of screening T1R1/T1R3 receptors for compounds that modulate umami taste signaling
US9476869B2 (en) 2001-04-19 2016-10-25 Senomyx, Inc. Methods of screening T1R hetero-oligomeric taste receptors for sweet taste modulators
US8895050B2 (en) 2003-08-06 2014-11-25 Senomyx, Inc. Flavors, flavor modifiers, tastants, taste enhancers, umami or sweet tastants, and/or enhancers and use thereof

Also Published As

Publication number Publication date
CA2452716C (en) 2012-06-26
JP2009077718A (en) 2009-04-16
EP1585954B1 (en) 2014-03-26
US20030166137A1 (en) 2003-09-04
US20140024043A1 (en) 2014-01-23
EP1585954A4 (en) 2008-01-02
AU2009206153B2 (en) 2012-01-12
US7588900B2 (en) 2009-09-15
US20080318251A1 (en) 2008-12-25
WO2003004992A2 (en) 2003-01-16
US9347935B2 (en) 2016-05-24
US20110151483A1 (en) 2011-06-23
US8470970B2 (en) 2013-06-25
JP2005505262A (en) 2005-02-24
AU2009206153A1 (en) 2009-08-27
CA2452716A1 (en) 2003-01-16
US7842786B2 (en) 2010-11-30
JP2011217752A (en) 2011-11-04
AU2002320294B2 (en) 2009-01-15
EP1585954A2 (en) 2005-10-19

Similar Documents

Publication Publication Date Title
WO2003004992A3 (en) Mammalian sweet and amino acid heterodimeric taste receptors
WO2001018050A3 (en) T2r taste receptor family
WO2005033125A3 (en) Mammalian t1r3 sweet taste receptors
HK1101639A1 (en) Nucleic acids encoding a g-protein coupled receptor involved in sensory transduction
WO2002028901A3 (en) Seven-transmembrane (7tm) receptors and uses thereof
WO2004014311A3 (en) Nogo receptor antagonists
WO2002044426A3 (en) Nod2 nucleic acids and proteins
CY1111097T1 (en) NOGO RECEPTOR'S COMMITMENT PROTEIN
WO2001064882A3 (en) 1983, 52881, 2398, 45449, 50289, and 52872, g protein-coupled receptors and uses therefor
WO2005016955A3 (en) Nogo receptor antagonists
WO2003025137A3 (en) Sweet taste receptors
WO2002042458A3 (en) G protein coupled receptors
WO2002000719A3 (en) Human and mouse g-protein couipled receptors
WO2001085791A8 (en) Nucleic acid sequences for novel gpcrs
WO2003008627A3 (en) Phenylthiocarbamide (ptc) taste receptor
WO2004090103A3 (en) Identification of novel nogo-receptors and methods related thereto
WO2003004678A3 (en) Novel receptors
WO2002068591A3 (en) G-protein coupled receptor nucleic acids, polypeptides, antibodies and uses thereof
WO2003034275A3 (en) Chimeric glycosylphosphatidylinositol containing peptides
WO2005118647A3 (en) M13 virus major coat protein variants for c-terminal and bi-terminal display of a heterologous protein
WO2005100386A3 (en) Canine cold-and menthol-sensitive receptor 1
WO2002074960A3 (en) 38650, 28472, 5495, 65507, 81588 and 14354 methods and compositions of human proteins and uses thereof
WO2003052078A3 (en) Insect g protein-coupled receptor genes and uses thereof
WO2003021262A1 (en) Screening method
WO2001068704A3 (en) G-protein coupled receptors

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR IE IT LU MC NL PT SE SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG US

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2452716

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2003510918

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2002320294

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2002749804

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWP Wipo information: published in national office

Ref document number: 2002749804

Country of ref document: EP