WO2003011768A3 - Microfluidic device for molecular analysis - Google Patents

Microfluidic device for molecular analysis Download PDF

Info

Publication number
WO2003011768A3
WO2003011768A3 PCT/US2002/024316 US0224316W WO03011768A3 WO 2003011768 A3 WO2003011768 A3 WO 2003011768A3 US 0224316 W US0224316 W US 0224316W WO 03011768 A3 WO03011768 A3 WO 03011768A3
Authority
WO
WIPO (PCT)
Prior art keywords
signal delivery
microfluidic device
sample
detection
molecules
Prior art date
Application number
PCT/US2002/024316
Other languages
French (fr)
Other versions
WO2003011768A2 (en
Inventor
Thor W Nilsen
Original Assignee
Datascope Investment Corp
Thor W Nilsen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Datascope Investment Corp, Thor W Nilsen filed Critical Datascope Investment Corp
Publication of WO2003011768A2 publication Critical patent/WO2003011768A2/en
Publication of WO2003011768A3 publication Critical patent/WO2003011768A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow

Abstract

An improved microfluidic device for the detection and analysis of desired chemical or biochemical components. The device allows molecular biological analysis and diagnosis using multistep reactions conducted on a small scale, with such reactions being conducted on or preferably within an electronic chip (12) containing biochemical components that enable the detection of desired materials in an applied sample. Preferably, the device includes a sample chamber (26) for lysis of sample cells using an applied AC field, a signal delivery chamber (46) wherein signal delivery molecules are bound to desired target molecules of interest, and/or electrodes (90) for detecting signal produced by the signal delivery molecules.
PCT/US2002/024316 2001-08-01 2002-08-01 Microfluidic device for molecular analysis WO2003011768A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30922101P 2001-08-01 2001-08-01
US60/309,221 2001-08-01

Publications (2)

Publication Number Publication Date
WO2003011768A2 WO2003011768A2 (en) 2003-02-13
WO2003011768A3 true WO2003011768A3 (en) 2003-05-30

Family

ID=23197238

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/024316 WO2003011768A2 (en) 2001-08-01 2002-08-01 Microfluidic device for molecular analysis

Country Status (1)

Country Link
WO (1) WO2003011768A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10353985A1 (en) * 2003-11-19 2005-06-23 Olympus Biosystems Gmbh Apparatus for manipulation and analysis of micro-objects, useful particularly for cells or their components, is constructed as a fluidics microsystem and/or microchip
GB2416030B (en) * 2004-01-28 2008-07-23 Norchip As A diagnostic system for carrying out a nucleic acid sequence amplification and detection process
US7488596B2 (en) * 2004-12-17 2009-02-10 Samsung Electronics Co., Ltd. Microfluidic device comprising electrolysis device for cell lysis and method for electrochemically lysing cells using the same
US20120070842A1 (en) * 2009-06-01 2012-03-22 Harley Calvin B Assay for Telomerase Activity Using Microfluidic Device
DE102010064391A1 (en) * 2010-10-29 2012-05-03 Endress + Hauser Conducta Gesellschaft für Mess- und Regeltechnik mbH + Co. KG Method for determining an analyte content of a liquid sample by means of a bioanalyzer
WO2012064704A1 (en) * 2010-11-08 2012-05-18 Daktari Diagnostics, Inc. Multi-function microfluidic test kit
ITMI20131541A1 (en) * 2013-09-19 2015-03-20 Eugenio Iannone DIAGNOSIS DEVICE, PARTICULARLY OF THE LAB-ON-CHIP TYPE.
CN104897756B (en) * 2015-06-19 2015-12-02 青岛大学 A kind of electrochemical sensor for test side telomerase activity and preparation method thereof
US10351893B2 (en) * 2015-10-05 2019-07-16 GeneWeave Biosciences, Inc. Reagent cartridge for detection of cells
WO2018051374A1 (en) * 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
WO2023223331A1 (en) * 2022-05-18 2023-11-23 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Institute) Dual-mode electrochemical point-of-care detection, quantification and profiling of pathogens

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6174675B1 (en) * 1997-11-25 2001-01-16 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6264825B1 (en) * 1998-06-23 2001-07-24 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes
US6344326B1 (en) * 1996-07-30 2002-02-05 Aclara Bio Sciences, Inc. Microfluidic method for nucleic acid purification and processing
US6406893B1 (en) * 1997-04-04 2002-06-18 Caliper Technologies Corp. Microfluidic methods for non-thermal nucleic acid manipulations

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6344326B1 (en) * 1996-07-30 2002-02-05 Aclara Bio Sciences, Inc. Microfluidic method for nucleic acid purification and processing
US6406893B1 (en) * 1997-04-04 2002-06-18 Caliper Technologies Corp. Microfluidic methods for non-thermal nucleic acid manipulations
US6174675B1 (en) * 1997-11-25 2001-01-16 Caliper Technologies Corp. Electrical current for controlling fluid parameters in microchannels
US6264825B1 (en) * 1998-06-23 2001-07-24 Clinical Micro Sensors, Inc. Binding acceleration techniques for the detection of analytes

Also Published As

Publication number Publication date
WO2003011768A2 (en) 2003-02-13

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