WO2003016832A3 - Microfluidic mixing and dispensing - Google Patents

Microfluidic mixing and dispensing Download PDF

Info

Publication number
WO2003016832A3
WO2003016832A3 PCT/US2002/025653 US0225653W WO03016832A3 WO 2003016832 A3 WO2003016832 A3 WO 2003016832A3 US 0225653 W US0225653 W US 0225653W WO 03016832 A3 WO03016832 A3 WO 03016832A3
Authority
WO
WIPO (PCT)
Prior art keywords
tube
dispensing
aperture
seal
dose
Prior art date
Application number
PCT/US2002/025653
Other languages
French (fr)
Other versions
WO2003016832A2 (en
Inventor
Jeffrey A Karg
Douglas W Kroncke
Original Assignee
Boston Innovation Inc
Jeffrey A Karg
Douglas W Kroncke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston Innovation Inc, Jeffrey A Karg, Douglas W Kroncke filed Critical Boston Innovation Inc
Priority to AU2002324687A priority Critical patent/AU2002324687A1/en
Publication of WO2003016832A2 publication Critical patent/WO2003016832A2/en
Publication of WO2003016832A3 publication Critical patent/WO2003016832A3/en
Priority to US10/778,776 priority patent/US7459128B2/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0241—Drop counters; Drop formers
    • B01L3/0265—Drop counters; Drop formers using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433—Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/40—Static mixers
    • B01F25/42—Static mixers in which the mixing is affected by moving the components jointly in changing directions, e.g. in tubes provided with baffles or obstructions
    • B01F25/43—Mixing tubes, e.g. wherein the material is moved in a radial or partly reversed direction
    • B01F25/433—Mixing tubes wherein the shape of the tube influences the mixing, e.g. mixing tubes with varying cross-section or provided with inwardly extending profiles
    • B01F25/4335—Mixers with a converging-diverging cross-section
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/65—Mixers with shaking, oscillating, or vibrating mechanisms the materials to be mixed being directly submitted to a pulsating movement, e.g. by means of an oscillating piston or air column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00306—Reactor vessels in a multiple arrangement
    • B01J2219/00313—Reactor vessels in a multiple arrangement the reactor vessels being formed by arrays of wells in blocks
    • B01J2219/00315—Microtiter plates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/0036—Nozzles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00364—Pipettes
    • B01J2219/00367—Pipettes capillary
    • B01J2219/00369—Pipettes capillary in multiple or parallel arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/065—Valves, specific forms thereof with moving parts sliding valves
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1032—Dilution or aliquotting
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065—Multiple transfer devices
    • G01N35/1074—Multiple transfer devices arranged in a two-dimensional array

Abstract

A micro fluidic dispensing tap (20), such as for use in screening assays, includes a 5 dispensing tube (11) translatable within a sealed reservoir housing (32), The tube has an outer surface and defines an inner cavity (25) open at a lower end of the tube, and a metering aperture (27) extending through a side wall of the tube between the inner cavity and the outer surface to define a known volume. The tube is movable against a seal (24) at a lower end of the reservoir housing between a first position, in which the metering aperture is disposed 10 below the seal, and a second position, in which the metering aperture is disposed above the seal and exposed to the reservoir (21) for entraining a discrete dose of a sample liquid (30) within the aperture. An injector (130) is configured to inject a known quantity ofa diluent (53) into the inner cavity of the tube and into fluidic contact with the dose of sample liquid in the aperture, such that the dose of sample liquid diffuses into the diluent to form a discrete 15 mixture for dispensing from an open end of the tube.
PCT/US2002/025653 2001-08-13 2002-08-13 Microfluidic mixing and dispensing WO2003016832A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002324687A AU2002324687A1 (en) 2001-08-13 2002-08-13 Microfluidic mixing and dispensing
US10/778,776 US7459128B2 (en) 2002-08-13 2004-02-13 Microfluidic mixing and dispensing

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US31185901P 2001-08-13 2001-08-13
US60/311,859 2001-08-13

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/778,776 Continuation US7459128B2 (en) 2002-08-13 2004-02-13 Microfluidic mixing and dispensing

Publications (2)

Publication Number Publication Date
WO2003016832A2 WO2003016832A2 (en) 2003-02-27
WO2003016832A3 true WO2003016832A3 (en) 2003-05-30

Family

ID=23208825

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/025653 WO2003016832A2 (en) 2001-08-13 2002-08-13 Microfluidic mixing and dispensing

Country Status (2)

Country Link
AU (1) AU2002324687A1 (en)
WO (1) WO2003016832A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7459128B2 (en) 2002-08-13 2008-12-02 Molecular Bioproducts, Inc. Microfluidic mixing and dispensing
US8409528B2 (en) 2003-06-19 2013-04-02 Abbott Laboratories Apparatus and method for handling fluids for analysis
EP1681571B8 (en) * 2003-06-19 2010-06-23 Abbott Laboratories Apparatus and method for handling fluids for analysis
DE10360269A1 (en) * 2003-12-17 2005-07-28 Friedrich-Schiller-Universität Jena To mix fluids together in small volumes without mechanical movement e.g. at micro-titration plates, for analysis, a reagent is added with a lower surface tension and a high concentration gradient to generate a high heat of solution
US7592185B2 (en) 2004-02-17 2009-09-22 Molecular Bioproducts, Inc. Metering doses of sample liquids
US8127794B2 (en) 2006-09-19 2012-03-06 Agency For Science, Technology And Research Dispenser arrangement for fluidic dispensing control in microfluidic system
US20100047898A1 (en) * 2008-08-19 2010-02-25 Biomerieux, Inc. Mixing pipette
CN111624085B (en) * 2020-07-29 2020-10-23 天津中新科炬生物制药股份有限公司 Sample diluting device and reagent card for detecting novel coronavirus antibody
CN114108105B (en) * 2021-11-04 2023-08-25 杭州奥盛仪器有限公司 Full-automatic NGS library construction system

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3115568A1 (en) * 1980-04-18 1982-04-15 Beckman Instruments Inc., 92634 Fullerton, Calif. Method and apparatus for mixing liquids using an automated pipette
US4844868A (en) * 1985-06-18 1989-07-04 Kabushiki Kaisha Toshiba Automatic chemical analysis reagent distribution and analysis apparatus
WO1990008075A1 (en) * 1989-01-12 1990-07-26 Microvol Limited Pressurised metering dispenser
EP0971235A1 (en) * 1998-07-10 2000-01-12 Bayer Corporation Sample dilution module with offset mixing chamber
US6165417A (en) * 1998-10-26 2000-12-26 The Regents Of The University Of California Integrated titer plate-injector head for microdrop array preparation, storage and transfer
WO2001064345A2 (en) * 2000-02-29 2001-09-07 Boston Innovation, Inc. Microvolume liquid dispensing

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3115568A1 (en) * 1980-04-18 1982-04-15 Beckman Instruments Inc., 92634 Fullerton, Calif. Method and apparatus for mixing liquids using an automated pipette
US4844868A (en) * 1985-06-18 1989-07-04 Kabushiki Kaisha Toshiba Automatic chemical analysis reagent distribution and analysis apparatus
WO1990008075A1 (en) * 1989-01-12 1990-07-26 Microvol Limited Pressurised metering dispenser
EP0971235A1 (en) * 1998-07-10 2000-01-12 Bayer Corporation Sample dilution module with offset mixing chamber
US6165417A (en) * 1998-10-26 2000-12-26 The Regents Of The University Of California Integrated titer plate-injector head for microdrop array preparation, storage and transfer
WO2001064345A2 (en) * 2000-02-29 2001-09-07 Boston Innovation, Inc. Microvolume liquid dispensing

Also Published As

Publication number Publication date
WO2003016832A2 (en) 2003-02-27
AU2002324687A1 (en) 2003-03-03

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