WO2003027238A3 - Stabilization of t4 endonuclease v - Google Patents

Stabilization of t4 endonuclease v Download PDF

Info

Publication number
WO2003027238A3
WO2003027238A3 PCT/US2002/029885 US0229885W WO03027238A3 WO 2003027238 A3 WO2003027238 A3 WO 2003027238A3 US 0229885 W US0229885 W US 0229885W WO 03027238 A3 WO03027238 A3 WO 03027238A3
Authority
WO
WIPO (PCT)
Prior art keywords
endonuclease
activity
stabilization
methods
cryptic
Prior art date
Application number
PCT/US2002/029885
Other languages
French (fr)
Other versions
WO2003027238A2 (en
Inventor
Anne B Brown
David A Brown
Daniel B Yarosh
Original Assignee
Applied Genetics Inc Dermatics
Anne B Brown
David A Brown
Daniel B Yarosh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Genetics Inc Dermatics, Anne B Brown, David A Brown, Daniel B Yarosh filed Critical Applied Genetics Inc Dermatics
Priority to JP2003530810A priority Critical patent/JP4283111B2/en
Priority to AU2002339973A priority patent/AU2002339973B2/en
Priority to EP02778299A priority patent/EP1430143A4/en
Priority to CA002461019A priority patent/CA2461019A1/en
Priority to US10/490,397 priority patent/US7094560B2/en
Publication of WO2003027238A2 publication Critical patent/WO2003027238A2/en
Publication of WO2003027238A3 publication Critical patent/WO2003027238A3/en
Priority to US11/507,213 priority patent/US20060281166A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase

Abstract

The invention provides T4 endonuclease V compositions that exhibit enhanced stability, including stability at non-refrigerated temperatures, through reduced activity of cryptic proteases. Methods for detecting cryptic protease activity and methods for reducing such activity are also provided.
PCT/US2002/029885 2001-09-24 2002-09-20 Stabilization of t4 endonuclease v WO2003027238A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
JP2003530810A JP4283111B2 (en) 2001-09-24 2002-09-20 Stabilization of T4 endonuclease
AU2002339973A AU2002339973B2 (en) 2001-09-24 2002-09-20 Stabilization of T4 endonuclease V
EP02778299A EP1430143A4 (en) 2001-09-24 2002-09-20 Stabilization of t4 endonuclease v
CA002461019A CA2461019A1 (en) 2001-09-24 2002-09-20 Stabilization of t4 endonuclease v
US10/490,397 US7094560B2 (en) 2001-09-24 2002-09-20 Stabilization of T4 endonuclease V
US11/507,213 US20060281166A1 (en) 2001-09-24 2006-08-21 Stabilization of T4 endonuclease V

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US32444801P 2001-09-24 2001-09-24
US60/324,448 2001-09-24

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US11/507,213 Continuation US20060281166A1 (en) 2001-09-24 2006-08-21 Stabilization of T4 endonuclease V

Publications (2)

Publication Number Publication Date
WO2003027238A2 WO2003027238A2 (en) 2003-04-03
WO2003027238A3 true WO2003027238A3 (en) 2004-02-12

Family

ID=23263628

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/029885 WO2003027238A2 (en) 2001-09-24 2002-09-20 Stabilization of t4 endonuclease v

Country Status (6)

Country Link
US (2) US7094560B2 (en)
EP (1) EP1430143A4 (en)
JP (1) JP4283111B2 (en)
AU (1) AU2002339973B2 (en)
CA (1) CA2461019A1 (en)
WO (1) WO2003027238A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5272079A (en) * 1988-07-06 1993-12-21 Applied Genetics, Inc. Purification and administration of DNA repair enzymes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190762A (en) 1988-07-06 1993-03-02 Applied Genetics, Inc. Method of administering proteins to living skin cells
US5077211A (en) 1988-07-06 1991-12-31 Applied Genetics, Inc. Purification and administration of dna repair enzymes
US5141852A (en) 1988-12-12 1992-08-25 The United States Of America As Represented By The Department Of Health And Human Services Assay of protein kinases with peptide substrates
US5616311A (en) 1991-01-15 1997-04-01 Hemosphere, Inc. Non-crosslinked protein particles for therapeutic and diagnostic use
WO1993010461A1 (en) * 1991-11-12 1993-05-27 Promega Corporation Non-radioactive enzyme assay
US5302389A (en) 1992-08-17 1994-04-12 Board Of Regents, The University Of Texas System Method for treating UV-induced suppression of contact hypersensitivity by administration of T4 endonuclease
US5352458A (en) 1992-12-21 1994-10-04 Applied Genetics Inc. Tanning method using DNA repair liposomes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5272079A (en) * 1988-07-06 1993-12-21 Applied Genetics, Inc. Purification and administration of DNA repair enzymes

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
DODSON M.L. ET AL.: "Site-directed deletion mutagenesis within the T4 endonuclease V gene: dispensable sequences within putative loop regions", MUTA. RES., DNA REPAIR, vol. 255, 1991, pages 19 - 29, XP002965458 *
See also references of EP1430143A4 *
YAROSH D.B. ET AL.: "Photoprotection by topical DNA repair enzymes: molecular correlates of clinical studies", PHOTOCHEM. PHOTOBIOL., vol. 69, no. 2, 1999, pages 136 - 140, XP002965457 *

Also Published As

Publication number Publication date
US20040248230A1 (en) 2004-12-09
WO2003027238A2 (en) 2003-04-03
EP1430143A2 (en) 2004-06-23
CA2461019A1 (en) 2003-04-03
JP4283111B2 (en) 2009-06-24
AU2002339973B2 (en) 2007-10-18
EP1430143A4 (en) 2005-07-13
US7094560B2 (en) 2006-08-22
US20060281166A1 (en) 2006-12-14
JP2005503806A (en) 2005-02-10

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