WO2003046219A3 - Amperometric affinity assay and electrically stimulated complexes of nucleic acids - Google Patents

Amperometric affinity assay and electrically stimulated complexes of nucleic acids Download PDF

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Publication number
WO2003046219A3
WO2003046219A3 PCT/IB2002/004858 IB0204858W WO03046219A3 WO 2003046219 A3 WO2003046219 A3 WO 2003046219A3 IB 0204858 W IB0204858 W IB 0204858W WO 03046219 A3 WO03046219 A3 WO 03046219A3
Authority
WO
WIPO (PCT)
Prior art keywords
medium
nucleobase
containing sequence
test
amperometric
Prior art date
Application number
PCT/IB2002/004858
Other languages
French (fr)
Other versions
WO2003046219A2 (en
Inventor
Glen H Erikson
Original Assignee
Ingeneus Corp
Glen H Erikson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ingeneus Corp, Glen H Erikson filed Critical Ingeneus Corp
Priority to AU2002347469A priority Critical patent/AU2002347469A1/en
Publication of WO2003046219A2 publication Critical patent/WO2003046219A2/en
Publication of WO2003046219A3 publication Critical patent/WO2003046219A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/02—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance
    • G01N27/04—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance
    • G01N27/06—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating impedance by investigating resistance of a liquid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

A method for determining an affinity of a first nucleobase-containing sequence for a second nucleobase containing sequence includes providing a test medium containing the first nucleobase containing sequence and the second nucleobase containing sequence, applying a voltage across the test medium, measuring a test electric current through the test medium; and determining the affinity by evaluating whether the test electric current is equivalent to a reference electric current of a reference medium containing a longer of the first nucleobase containing sequence and the second nucleobase containing sequence. A complex in an electrically-stimulated phase contains at least two nucleobase containing sequences in a medium, wherein the electrical conductivity of the medium increases linearly without a plateau as the temperature of the medium approaches and exceeds a Tm of the complex.
PCT/IB2002/004858 2001-11-29 2002-11-18 Amperometric affinity assay and electrically stimulated complexes of nucleic acids WO2003046219A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002347469A AU2002347469A1 (en) 2001-11-29 2002-11-18 Amperometric affinity assay and electrically stimulated complexes of nucleic acids

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/998,155 2001-11-29
US09/998,155 US6613524B1 (en) 2000-01-24 2001-11-29 Amperometric affinity assay and electrically stimulated complexes of nucleic acids

Publications (2)

Publication Number Publication Date
WO2003046219A2 WO2003046219A2 (en) 2003-06-05
WO2003046219A3 true WO2003046219A3 (en) 2003-10-16

Family

ID=25544844

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2002/004858 WO2003046219A2 (en) 2001-11-29 2002-11-18 Amperometric affinity assay and electrically stimulated complexes of nucleic acids

Country Status (4)

Country Link
US (2) US6613524B1 (en)
AU (1) AU2002347469A1 (en)
TW (1) TWI257427B (en)
WO (1) WO2003046219A2 (en)

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US7052844B2 (en) * 1999-12-21 2006-05-30 Ingeneus, Inc. Purification of DS-DNA using heteropolymeric capture probes and a triplex, quadruplex or homologous duplex binding mechanism
US7220541B2 (en) * 2000-01-24 2007-05-22 Ingeneus, Inc. Homogeneous assay of biopolymer binding by means of multiple measurements under varied conditions
US20030170659A1 (en) * 2000-01-24 2003-09-11 Ingeneus Corporation Electrical treatment of binding media to encourage, discourage and/or study biopolymer binding
US6613524B1 (en) * 2000-01-24 2003-09-02 Ingeneus Corporation Amperometric affinity assay and electrically stimulated complexes of nucleic acids
US6982147B2 (en) * 2000-01-24 2006-01-03 Ingeneus Corporation Apparatus for assaying biopolymer binding by means of multiple measurements under varied conditions
US20040142329A1 (en) * 2003-01-17 2004-07-22 Ingeneus Corporation Probe conjugation to increase multiplex binding motif preference
US20040180345A1 (en) * 2003-03-14 2004-09-16 Ingeneus Corporation Pre-incubation method to improve signal/noise ratio of nucleic acid assays
BRPI0516955A (en) * 2004-09-24 2008-09-30 Ingeneus Inc genetic assay
KR101118461B1 (en) * 2008-10-10 2012-03-06 나노칩스 (주) Ultrafast dna structures analyzing system with high detectivity
BR112013022757A2 (en) 2011-03-09 2021-01-05 Chong Corporation DRUG DELIVERY SYSTEM

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WO2001053526A2 (en) * 2000-01-24 2001-07-26 Ingeneus Corporation Homogenous assay of duplex or triplex hybridization by means of multiple measurements under varied conditions

Also Published As

Publication number Publication date
US20030180790A1 (en) 2003-09-25
TWI257427B (en) 2006-07-01
US6613524B1 (en) 2003-09-02
TW200300452A (en) 2003-06-01
WO2003046219A2 (en) 2003-06-05
AU2002347469A1 (en) 2003-06-10

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