WO2003048770A3 - Procede pour effectuer un diagnostic in vitro au moyen de mecanismes de regulation genetique et trousse de diagnostic correspondante - Google Patents

Procede pour effectuer un diagnostic in vitro au moyen de mecanismes de regulation genetique et trousse de diagnostic correspondante Download PDF

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Publication number
WO2003048770A3
WO2003048770A3 PCT/BE2002/000183 BE0200183W WO03048770A3 WO 2003048770 A3 WO2003048770 A3 WO 2003048770A3 BE 0200183 W BE0200183 W BE 0200183W WO 03048770 A3 WO03048770 A3 WO 03048770A3
Authority
WO
WIPO (PCT)
Prior art keywords
diagnosis
kit
gene regulation
ligand
receptor
Prior art date
Application number
PCT/BE2002/000183
Other languages
English (en)
Other versions
WO2003048770A2 (fr
Inventor
Benoit Granier
Sophie Lepage
Original Assignee
Unisensor S A
Benoit Granier
Sophie Lepage
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unisensor S A, Benoit Granier, Sophie Lepage filed Critical Unisensor S A
Priority to US10/496,747 priority Critical patent/US7935806B2/en
Priority to ES02782543T priority patent/ES2394200T3/es
Priority to EP02782543A priority patent/EP1451583B1/fr
Priority to DK02782543.9T priority patent/DK1451583T3/da
Priority to AU2002347226A priority patent/AU2002347226A1/en
Publication of WO2003048770A2 publication Critical patent/WO2003048770A2/fr
Publication of WO2003048770A3 publication Critical patent/WO2003048770A3/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • G01N33/9446—Antibacterials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/70567—Nuclear receptors, e.g. retinoic acid receptor [RAR], RXR, nuclear orphan receptors

Abstract

La présente invention se rapporte à une trousse de détection et/ou de quantification d'un ligand (C), présent dans un échantillon d'analyse, par un récepteur (A), caractérisée en ce qu'elle comprend les réactifs suivants: une séquence nucléotidique (B) simple brin ou double brin, et; ledit récepteur (A) constitué d'une entité protéique monomérique ou multimérique comprenant au moins un premier site de reconnaissance spécifique pour ledit ligand (C) et un deuxième site de reconnaissance spécifique pour ladite séquence nucléotidique (B); ledit ligand (C) étant capable de moduler positivement ou négativement la fixation du récepteur (A) par son site de reconnaissance spécifique sur la séquence nucléotidique (B).
PCT/BE2002/000183 2001-12-06 2002-12-04 Procede pour effectuer un diagnostic in vitro au moyen de mecanismes de regulation genetique et trousse de diagnostic correspondante WO2003048770A2 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
US10/496,747 US7935806B2 (en) 2001-12-06 2002-12-04 Method for performing in vitro diagnosis using gene regulation mechanisms and corresponding diagnosis kit
ES02782543T ES2394200T3 (es) 2001-12-06 2002-12-04 Procedimiento para la determinación de dosis de un antibiótico in vitro por medio de mecanismos de regulación genética y kit de diagnóstico correspondiente
EP02782543A EP1451583B1 (fr) 2001-12-06 2002-12-04 Procede pour le dosage d'un antibiotique in vitro au moyen de mecanismes de regulation genetique et trousse de diagnostic correspondante
DK02782543.9T DK1451583T3 (da) 2001-12-06 2002-12-04 Fremgangsmåde til analyse af et antibiotikum in vitro ved hjælp af genetiske reguleringsmekanismer samt dertil hørende diagnostisk sæt
AU2002347226A AU2002347226A1 (en) 2001-12-06 2002-12-04 Method for performing in vitro diagnosis using gene regulation mechanisms and corresponding diagnosis kit

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP01870273A EP1318400A1 (fr) 2001-12-06 2001-12-06 Procédé pour effectuer un diagnostic in vitro au moyen de mécanismes de régulation génétique et trousse de diagnostic correspondante
EP01870273.8 2001-12-06

Publications (2)

Publication Number Publication Date
WO2003048770A2 WO2003048770A2 (fr) 2003-06-12
WO2003048770A3 true WO2003048770A3 (fr) 2004-04-01

Family

ID=8185066

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/BE2002/000183 WO2003048770A2 (fr) 2001-12-06 2002-12-04 Procede pour effectuer un diagnostic in vitro au moyen de mecanismes de regulation genetique et trousse de diagnostic correspondante

Country Status (6)

Country Link
US (1) US7935806B2 (fr)
EP (2) EP1318400A1 (fr)
AU (1) AU2002347226A1 (fr)
DK (1) DK1451583T3 (fr)
ES (1) ES2394200T3 (fr)
WO (1) WO2003048770A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1712914A1 (fr) * 2005-04-14 2006-10-18 Unisensor S.A. Procédé in vitro pour la detection et l'identification simultanées d'antibiotiques de classes differentes et trousse de diagnostic correspondante
EP2058663A1 (fr) * 2007-11-12 2009-05-13 Euro-Diagnostica B.V. Procédé pour l'immobilisation d'un analyte sur un support solide
CN102680711A (zh) * 2012-05-25 2012-09-19 北京维德维康生物技术有限公司 与四环素结合的蛋白TetR在检测四环素类抗生素中的应用
CN103808939A (zh) * 2012-11-06 2014-05-21 江苏维赛科技生物发展有限公司 大观霉素胶体金检测卡
CN103353525A (zh) * 2012-12-12 2013-10-16 河南省农业科学院 金霉素残留检测试纸条及试纸卡
CN113957067B (zh) * 2021-10-12 2023-11-07 中国农业大学 基于TetR蛋白空间位阻和基因剪切技术的四环素类药物检测方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133027A (en) * 1996-08-07 2000-10-17 City Of Hope Inducible expression system

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5217867A (en) * 1988-11-30 1993-06-08 The Salk Institute For Biological Studies Receptors: their identification, characterization, preparation and use
US6025192A (en) * 1996-09-20 2000-02-15 Cold Spring Harbor Laboratory Modified retroviral vectors
US5854010A (en) 1997-03-10 1998-12-29 Denison; Michael S. Bioassay for detecting 2,3,7,8-tetrachlorodibenzo-para-dioxin and TCDD-like compounds and novel recombinant cell line useful therefor

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6133027A (en) * 1996-08-07 2000-10-17 City Of Hope Inducible expression system

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KURITTU, J. ET AL: "Qualitative detection of tetracycline residues in milk with a luminescence based microbial method: the effect of milk composition and assay performance in relation to an immunoassay and a microbial inhibition assay", JOURNAL OF FOOD PROTECION, vol. 63, no. 7, 2000, pages 953 - 957, XP001058083 *
ORTH, P. ET AL: "Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system", NATURE STRUCTURAL BIOLOGY, vol. 7, no. 3, March 2000 (2000-03-01), pages 215 - 219, XP001058071 *
POOK ELISABETH ET AL: "Affinities of mAbs to Tet repressor complexed with operator or tetracycline suggest conformational changes associated with induction.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 258, no. 3, December 1998 (1998-12-01), pages 915 - 922, XP001062176, ISSN: 0014-2956 *

Also Published As

Publication number Publication date
EP1318400A1 (fr) 2003-06-11
WO2003048770A2 (fr) 2003-06-12
US20050130152A1 (en) 2005-06-16
ES2394200T8 (es) 2013-02-25
US7935806B2 (en) 2011-05-03
AU2002347226A8 (en) 2003-06-17
DK1451583T3 (da) 2012-12-03
AU2002347226A1 (en) 2003-06-17
ES2394200T3 (es) 2013-01-23
EP1451583A2 (fr) 2004-09-01
EP1451583B1 (fr) 2012-08-29

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