WO2003052116A3 - Methods for detection of a target nucleic acid using multi-subunit probes - Google Patents

Methods for detection of a target nucleic acid using multi-subunit probes Download PDF

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Publication number
WO2003052116A3
WO2003052116A3 PCT/US2002/022721 US0222721W WO03052116A3 WO 2003052116 A3 WO2003052116 A3 WO 2003052116A3 US 0222721 W US0222721 W US 0222721W WO 03052116 A3 WO03052116 A3 WO 03052116A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
target nucleic
subunit
probe
detection
Prior art date
Application number
PCT/US2002/022721
Other languages
French (fr)
Other versions
WO2003052116A9 (en
WO2003052116A2 (en
Inventor
Joseph A Sorge
Original Assignee
Stratagene Inc
Joseph A Sorge
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stratagene Inc, Joseph A Sorge filed Critical Stratagene Inc
Priority to AU2002365028A priority Critical patent/AU2002365028A1/en
Publication of WO2003052116A2 publication Critical patent/WO2003052116A2/en
Publication of WO2003052116A3 publication Critical patent/WO2003052116A3/en
Publication of WO2003052116A9 publication Critical patent/WO2003052116A9/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Abstract

The invention relates to a method of generating a signal indicative of the presence of a target nucleic acid sequence in a sample, comprising forming a complex by incubating a sample comprising a target nucleic acid sequence with a probe comprising a first and second subunit, and/or an upstream primer, and binding the probe to the target nucleic acid such that the first and second subunits dissociate to release the first subunit and generate a signal. In certain embodiments, the upstream primer is extended with a nucleic acid polymerase to displace at least a portion of the first subunit of the probe from the target nucleic acid strand and dissociate the first and second subunits to release the first subunit of the probe and generate a signal.
PCT/US2002/022721 2001-07-17 2002-07-17 Methods for detection of a target nucleic acid using multi-subunit probes WO2003052116A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2002365028A AU2002365028A1 (en) 2001-07-17 2002-07-17 Methods for detection of a target nucleic acid using multi-subunit probes

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US30609001P 2001-07-17 2001-07-17
US60/306,090 2001-07-17
US30723801P 2001-07-23 2001-07-23
US60/307,238 2001-07-23
US31392101P 2001-08-21 2001-08-21
US60/313,921 2001-08-21

Publications (3)

Publication Number Publication Date
WO2003052116A2 WO2003052116A2 (en) 2003-06-26
WO2003052116A3 true WO2003052116A3 (en) 2003-12-11
WO2003052116A9 WO2003052116A9 (en) 2004-01-15

Family

ID=27405138

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/022721 WO2003052116A2 (en) 2001-07-17 2002-07-17 Methods for detection of a target nucleic acid using multi-subunit probes

Country Status (3)

Country Link
US (1) US7183052B2 (en)
AU (1) AU2002365028A1 (en)
WO (1) WO2003052116A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7838225B2 (en) * 1999-10-29 2010-11-23 Hologic, Inc. Methods for detection of a target nucleic acid by forming a cleavage structure using a reverse transcriptase
US7824859B2 (en) * 1999-10-29 2010-11-02 Cytyc Corporation Methods for detection of a target nucleic acid by forming a cleavage structure using an RNA polymerase
US7118860B2 (en) * 1999-10-29 2006-10-10 Stratagene California Methods for detection of a target nucleic acid by capture
EP1430150B1 (en) 2001-09-24 2015-08-19 One Lambda, Inc. Diagnostic probe detection system
KR100475312B1 (en) * 2002-12-10 2005-03-10 한국전자통신연구원 Chips for detecting DNA and method for detecting DNA using them
CN1860241A (en) * 2003-07-30 2006-11-08 财团法人理工学振兴会 Partially double stranded nucleic acid molecule
CA2502549C (en) * 2004-04-23 2016-02-16 Becton, Dickinson And Company Use of an extraction control in a method of extracting nucleic acids
US9121056B2 (en) * 2006-04-28 2015-09-01 Igor Kutyavin Use of products of PCR amplification carrying elements of secondary structure to improve PCR-based nucleic acid detection
US7772390B1 (en) 2006-07-18 2010-08-10 The Regents Of The University Of California Lipid mediated nucleic acid synthesis
US9222936B2 (en) 2007-04-18 2015-12-29 Solulink, Inc. Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization
US20130184184A1 (en) * 2010-11-22 2013-07-18 The University Of Chicago Methods and/or Use of Oligonucleotide Conjugates Having Varied Degrees of Labeling for Assays and Detections
EP2820157B1 (en) 2012-03-02 2019-05-01 Winthrop-University Hospital Method for using probe based pcr detection to measure the levels of circulating demethylated beta cell derived dna as a measure of beta cell loss in diabetes
SG10201704633UA (en) 2012-12-10 2017-07-28 Resolution Bioscience Inc Methods for targeted genomic analysis
CA3004435A1 (en) 2015-11-11 2017-05-18 Resolution Bioscience, Inc. High efficiency construction of dna libraries
CA3034649A1 (en) 2016-08-25 2018-03-01 Resolution Bioscience, Inc. Methods for the detection of genomic copy changes in dna samples
JP2023542408A (en) * 2020-09-23 2023-10-06 マキュラ バイオテクノロジー カンパニー リミテッド Combinations, methods and kits for detecting nucleic acids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5846717A (en) * 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5614402A (en) * 1992-12-07 1997-03-25 Third Wave Technologies, Inc. 5' nucleases derived from thermostable DNA polymerase
US5719028A (en) * 1992-12-07 1998-02-17 Third Wave Technologies Inc. Cleavase fragment length polymorphism
US5888780A (en) * 1992-12-07 1999-03-30 Third Wave Technologies, Inc. Rapid detection and identification of nucleic acid variants
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
CA2252048C (en) * 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US6528254B1 (en) * 1999-10-29 2003-03-04 Stratagene Methods for detection of a target nucleic acid sequence
US6350580B1 (en) * 2000-10-11 2002-02-26 Stratagene Methods for detection of a target nucleic acid using a probe comprising secondary structure

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US5935791A (en) * 1997-09-23 1999-08-10 Becton, Dickinson And Company Detection of nucleic acids by fluorescence quenching

Also Published As

Publication number Publication date
AU2002365028A8 (en) 2003-06-30
US7183052B2 (en) 2007-02-27
WO2003052116A9 (en) 2004-01-15
AU2002365028A1 (en) 2003-06-30
WO2003052116A2 (en) 2003-06-26
US20030148310A1 (en) 2003-08-07

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