WO2003067249A3 - Capillary column chromatography process and system - Google Patents

Capillary column chromatography process and system Download PDF

Info

Publication number
WO2003067249A3
WO2003067249A3 PCT/US2003/001879 US0301879W WO03067249A3 WO 2003067249 A3 WO2003067249 A3 WO 2003067249A3 US 0301879 W US0301879 W US 0301879W WO 03067249 A3 WO03067249 A3 WO 03067249A3
Authority
WO
WIPO (PCT)
Prior art keywords
capillary
conduits
array
separation
fluid samples
Prior art date
Application number
PCT/US2003/001879
Other languages
French (fr)
Other versions
WO2003067249A2 (en
Inventor
H Paul Carlton
Original Assignee
Perseptive Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perseptive Biosystems Inc filed Critical Perseptive Biosystems Inc
Priority to JP2003566548A priority Critical patent/JP4157476B2/en
Priority to EP03737548.2A priority patent/EP1470416B1/en
Publication of WO2003067249A2 publication Critical patent/WO2003067249A2/en
Publication of WO2003067249A3 publication Critical patent/WO2003067249A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6095—Micromachined or nanomachined, e.g. micro- or nanosize
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • B01J20/285—Porous sorbents based on polymers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/82—Shaped bodies, e.g. monoliths, plugs, tubes, continuous beds
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00—Aspects relating to sorbent materials
    • B01J2220/80—Aspects related to sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J2220/84—Capillaries
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/52—Physical parameters
    • G01N2030/524—Physical parameters structural properties
    • G01N2030/528—Monolithic sorbent material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G01N30/56—Packing methods or coating methods
    • G01N2030/562—Packing methods or coating methods packing
    • G01N2030/565—Packing methods or coating methods packing slurry packing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/74—Optical detectors
    • G01N2030/746—Optical detectors detecting along the line of flow, e.g. axial
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/88—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
    • G01N2030/8809—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample
    • G01N2030/8813—Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86 analysis specially adapted for the sample biological materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/466—Flow patterns using more than one column with separation columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/50—Conditioning of the sorbent material or stationary liquid
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6004—Construction of the column end pieces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/60—Construction of the column
    • G01N30/6034—Construction of the column joining multiple columns
    • G01N30/6043—Construction of the column joining multiple columns in parallel
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph

Abstract

A microfluidic system is provided for separating components of many fluid samples in a parallel fashion through an array of capillary conduits. The system includes capillary conduits for receiving fluid samples, and for effecting separation of substituents of the sample by passing the sample through a corresponding array of capillary separation conduits containing a solid separation medium. The microfluidic circuit is made of three or more substrate layers and preferably includes etched channels in different layers that are dimensioned relative to one another to provide for retaining the solid composition within the capillary separation conduits. The system also includes an array of detector flow cells in fluid communication with the capillary separation conduits, and an array of high pressure connectors for connecting discrete capillary tubes to the fluid passages in the microfluidic circuit for introducing and removing the fluid samples from the system. The connections for the capillary tubes have a small footprint, are readily reversible (allowing ease of maintenance and replacement), and can seal against high pressures.
PCT/US2003/001879 2002-02-01 2003-01-21 Capillary column chromatography process and system WO2003067249A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2003566548A JP4157476B2 (en) 2002-02-01 2003-01-21 Capillary column chromatography process and system
EP03737548.2A EP1470416B1 (en) 2002-02-01 2003-01-21 Capillary column chromatography process and system

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/061,612 US6581441B1 (en) 2002-02-01 2002-02-01 Capillary column chromatography process and system
US10/061,612 2002-02-01

Publications (2)

Publication Number Publication Date
WO2003067249A2 WO2003067249A2 (en) 2003-08-14
WO2003067249A3 true WO2003067249A3 (en) 2003-10-16

Family

ID=22036915

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/001879 WO2003067249A2 (en) 2002-02-01 2003-01-21 Capillary column chromatography process and system

Country Status (4)

Country Link
US (2) US6581441B1 (en)
EP (2) EP2851683A3 (en)
JP (2) JP4157476B2 (en)
WO (1) WO2003067249A2 (en)

Families Citing this family (97)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US7027683B2 (en) * 2000-08-15 2006-04-11 Nanostream, Inc. Optical devices with fluidic systems
AU2002307152A1 (en) 2001-04-06 2002-10-21 California Institute Of Technology Nucleic acid amplification utilizing microfluidic devices
US6752922B2 (en) * 2001-04-06 2004-06-22 Fluidigm Corporation Microfluidic chromatography
US20020164816A1 (en) * 2001-04-06 2002-11-07 California Institute Of Technology Microfluidic sample separation device
US20020186263A1 (en) * 2001-06-07 2002-12-12 Nanostream, Inc. Microfluidic fraction collectors
US20020187557A1 (en) * 2001-06-07 2002-12-12 Hobbs Steven E. Systems and methods for introducing samples into microfluidic devices
US20030108664A1 (en) * 2001-10-05 2003-06-12 Kodas Toivo T. Methods and compositions for the formation of recessed electrical features on a substrate
US8440093B1 (en) 2001-10-26 2013-05-14 Fuidigm Corporation Methods and devices for electronic and magnetic sensing of the contents of microfluidic flow channels
EP1463796B1 (en) 2001-11-30 2013-01-09 Fluidigm Corporation Microfluidic device and methods of using same
US7691333B2 (en) 2001-11-30 2010-04-06 Fluidigm Corporation Microfluidic device and methods of using same
US6581441B1 (en) * 2002-02-01 2003-06-24 Perseptive Biosystems, Inc. Capillary column chromatography process and system
US6814859B2 (en) * 2002-02-13 2004-11-09 Nanostream, Inc. Frit material and bonding method for microfluidic separation devices
DE60303122T9 (en) * 2002-02-13 2007-02-22 Nanostream, Inc., Pasadena MICROFLUIDIC SEPARATION COLLONS AND THEIR MANUFACTURING PROCESS
US7261812B1 (en) 2002-02-13 2007-08-28 Nanostream, Inc. Multi-column separation devices and methods
US6958119B2 (en) * 2002-02-26 2005-10-25 Agilent Technologies, Inc. Mobile phase gradient generation microfluidic device
EP1499706A4 (en) 2002-04-01 2010-11-03 Fluidigm Corp Microfluidic particle-analysis systems
US6808644B2 (en) * 2002-05-02 2004-10-26 Institute Of Microelectronics Capillary with glass internal surface
US20030217923A1 (en) * 2002-05-24 2003-11-27 Harrison D. Jed Apparatus and method for trapping bead based reagents within microfluidic analysis systems
US20030223913A1 (en) * 2002-06-03 2003-12-04 Nanostream, Inc. Microfluidic separation devices and methods
US7214348B2 (en) * 2002-07-26 2007-05-08 Applera Corporation Microfluidic size-exclusion devices, systems, and methods
EP1551753A2 (en) 2002-09-25 2005-07-13 California Institute Of Technology Microfluidic large scale integration
JP5695287B2 (en) * 2002-10-02 2015-04-01 カリフォルニア インスティテュート オブ テクノロジー Nucleic acid analysis of microfluids
US20040092033A1 (en) * 2002-10-18 2004-05-13 Nanostream, Inc. Systems and methods for preparing microfluidic devices for operation
US7010964B2 (en) * 2002-10-31 2006-03-14 Nanostream, Inc. Pressurized microfluidic devices with optical detection regions
US6936167B2 (en) * 2002-10-31 2005-08-30 Nanostream, Inc. System and method for performing multiple parallel chromatographic separations
US20050048669A1 (en) * 2003-08-26 2005-03-03 Nanostream, Inc. Gasketless microfluidic device interface
AU2003287449A1 (en) * 2002-10-31 2004-05-25 Nanostream, Inc. Parallel detection chromatography systems
US6987263B2 (en) * 2002-12-13 2006-01-17 Nanostream, Inc. High throughput systems and methods for parallel sample analysis
US20060076482A1 (en) * 2002-12-13 2006-04-13 Hobbs Steven E High throughput systems and methods for parallel sample analysis
US7445926B2 (en) 2002-12-30 2008-11-04 The Regents Of The University Of California Fluid control structures in microfluidic devices
US7476363B2 (en) * 2003-04-03 2009-01-13 Fluidigm Corporation Microfluidic devices and methods of using same
US7666361B2 (en) * 2003-04-03 2010-02-23 Fluidigm Corporation Microfluidic devices and methods of using same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
US20050145496A1 (en) 2003-04-03 2005-07-07 Federico Goodsaid Thermal reaction device and method for using the same
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US7178386B1 (en) 2003-04-10 2007-02-20 Nanostream, Inc. Parallel fluid processing systems and methods
US7074327B2 (en) * 2003-05-08 2006-07-11 Nanostream, Inc. Sample preparation for parallel chromatography
US7015466B2 (en) * 2003-07-24 2006-03-21 Purdue Research Foundation Electrosonic spray ionization method and device for the atmospheric ionization of molecules
US7028536B2 (en) * 2004-06-29 2006-04-18 Nanostream, Inc. Sealing interface for microfluidic device
US20050032238A1 (en) * 2003-08-07 2005-02-10 Nanostream, Inc. Vented microfluidic separation devices and methods
US7413712B2 (en) 2003-08-11 2008-08-19 California Institute Of Technology Microfluidic rotary flow reactor matrix
US7407799B2 (en) * 2004-01-16 2008-08-05 California Institute Of Technology Microfluidic chemostat
JP4375031B2 (en) * 2004-01-28 2009-12-02 株式会社島津製作所 Microchip processing method and apparatus
WO2007021762A2 (en) 2005-08-09 2007-02-22 The University Of North Carolina At Chapel Hill Methods and materials for fabricating microfluidic devices
EP1716404A4 (en) * 2004-02-20 2010-05-05 Univ New York State Res Found Method and device for manipulating liquids in microfluidic systems
JP2005241456A (en) * 2004-02-26 2005-09-08 Sekisui Chem Co Ltd Liquid chromatograph for microreactors and microreactor using it
CN100390536C (en) * 2004-03-18 2008-05-28 复旦大学 High flux protein multidemension array chromatogram separating system
US7077175B2 (en) 2004-04-09 2006-07-18 Hongfeng Yin Particle packing of microdevice
US7419639B2 (en) * 2004-05-12 2008-09-02 The Board Of Trustees Of The Leland Stanford Junior University Multilayer microfluidic device
US20050257885A1 (en) * 2004-05-24 2005-11-24 Nanostream, Inc. Capillary multi-channel optical flow cell
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
GB2434001B (en) * 2004-08-18 2009-04-15 Waters Investments Ltd A modular flow cell for use in chromatography
EP1794581A2 (en) 2004-09-15 2007-06-13 Microchip Biotechnologies, Inc. Microfluidic devices
US20080135484A1 (en) * 2004-10-18 2008-06-12 Hammer Michael R Liquid Chromatography Apparatus
CA2507323A1 (en) * 2005-05-13 2006-11-13 Chromedx Inc. Diagnostic whole blood and plasma apparatus
CA2517299A1 (en) * 2005-08-26 2007-02-26 Chromedx Inc. Hollow needle assembly
US8206650B2 (en) * 2005-04-12 2012-06-26 Chromedx Inc. Joint-diagnostic spectroscopic and biosensor meter
US20100245803A1 (en) * 2005-04-12 2010-09-30 Chromedx Inc. Blood sample holder for spectroscopic analysis
US7740804B2 (en) * 2005-04-12 2010-06-22 Chromedx Inc. Spectroscopic sample holder
EP1941269B8 (en) * 2005-10-27 2015-12-30 Transientic Interactions AB A method of screening a biological target for weak interactions using weak affinity chromatography
US20070138076A1 (en) * 2005-12-16 2007-06-21 Fluidigm Corporation Devices and methods for microfluidic chromatography
US7749365B2 (en) 2006-02-01 2010-07-06 IntegenX, Inc. Optimized sample injection structures in microfluidic separations
JP5063616B2 (en) 2006-02-03 2012-10-31 インテジェニックス インコーポレイテッド Microfluidic device
US7766033B2 (en) * 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
EP1999465B1 (en) * 2006-03-24 2016-11-16 Waters Technologies Corporation Ceramic-based chromatography apparatus
US8841116B2 (en) * 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
US8501029B2 (en) * 2006-10-30 2013-08-06 Agilent Technologies, Inc. Micromachined titanium for high pressure microfluidic applications
WO2008064402A1 (en) * 2006-11-27 2008-06-05 Crocker Research Pty Ltd Downhole fluid property chromatography
CN101715483A (en) 2007-02-05 2010-05-26 微芯片生物工艺学股份有限公司 microfluidic and nanofluidic devices, systems, and applications
WO2009015296A1 (en) * 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
WO2009108260A2 (en) 2008-01-22 2009-09-03 Microchip Biotechnologies, Inc. Universal sample preparation system and use in an integrated analysis system
US8672532B2 (en) 2008-12-31 2014-03-18 Integenx Inc. Microfluidic methods
GB2467207A (en) * 2009-01-26 2010-07-28 Agilent Technologies Inc Separation device with moveable filing channel
WO2010102199A1 (en) * 2009-03-06 2010-09-10 Waters Technologies Corporation Electromechanical and fluidic interface to a microfluidic substrate
CN102459565A (en) 2009-06-02 2012-05-16 尹特根埃克斯有限公司 Fluidic devices with diaphragm valves
JP2012529268A (en) 2009-06-05 2012-11-22 インテジェンクス，インコーポレイテッド Use of universal sample preparation system and integrated analysis system
US20130041265A1 (en) * 2009-09-21 2013-02-14 Ron Sostek Methods and apparatus for introducing cells at a tissue site
US8507037B2 (en) * 2009-10-29 2013-08-13 Eastman Kodak Company Digital manufacture of an gas or liquid separation device
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP2577288B1 (en) 2010-06-07 2013-11-20 Commissariat à l'Énergie Atomique et aux Énergies Alternatives System for analyzing a gas mixture including at least one chromatography column
US8763642B2 (en) 2010-08-20 2014-07-01 Integenx Inc. Microfluidic devices with mechanically-sealed diaphragm valves
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
EP3848091A1 (en) 2011-03-07 2021-07-14 The Trustees of Columbia University in the City of New York Apparatus, method, and system for selectively effecting and/or killing bacteria
US20180169279A1 (en) 2011-03-07 2018-06-21 The Trustees Of Columbia University In The City Of New York Apparatus, method and system for selectively affecting and/or killing a virus
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
WO2015073999A1 (en) 2013-11-18 2015-05-21 Integenx Inc. Cartridges and instruments for sample analysis
GB2544198B (en) 2014-05-21 2021-01-13 Integenx Inc Fluidic cartridge with valve mechanism
CN107106983B (en) 2014-10-22 2021-04-16 尹特根埃克斯有限公司 Systems and methods for sample preparation, processing, and analysis
EP3338084A4 (en) * 2015-08-21 2019-04-03 Deakin University A microfluidic device and methods for manufacturing same
US10295512B2 (en) 2015-12-08 2019-05-21 Dionex Corporation Multi-lumen mixing device for chromatography
WO2019209776A1 (en) * 2018-04-27 2019-10-31 Corning Incorporated Microfluidic devices and methods for manufacturing microfluidic devices
JP2021079334A (en) * 2019-11-19 2021-05-27 株式会社エンプラス Fluid handling apparatus
WO2021166255A1 (en) * 2020-02-21 2021-08-26 株式会社島津製作所 Liquid chromatograph and analysis method

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483773A (en) * 1982-10-04 1984-11-20 Varian Associates, Inc. Narrow bore micro-particulate column packing process and product
US5348658A (en) * 1990-05-29 1994-09-20 Waters Investments Limited Process for effecting capillary electrophoresis
WO2000025128A1 (en) * 1998-10-23 2000-05-04 Battelle Memorial Institute Method and apparatus for packed column separations and purifications
WO2001038865A1 (en) * 1999-11-26 2001-05-31 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4798089A (en) * 1987-03-12 1989-01-17 Rosemount Inc. Isolator apparatus
EP0412301B1 (en) * 1989-07-11 1994-12-07 Redwood MicroSystems, Inc. Boron nitride membrane in wafer structure
SE470347B (en) * 1990-05-10 1994-01-31 Pharmacia Lkb Biotech Microstructure for fluid flow systems and process for manufacturing such a system
EP0459241B1 (en) * 1990-05-29 1994-10-26 Waters Investments Limited Process and apparatus for effecting capillary electrophoresis
US5095938A (en) * 1990-12-21 1992-03-17 Millipore Corporation Injector for fluid delivery system
US5130343A (en) 1991-03-13 1992-07-14 Cornell Research Foundation, Inc. Process for producing uniform macroporous polymer beads
JP3168006B2 (en) * 1991-10-21 2001-05-21 コーネル・リサーチ・フアウンデーシヨン・インコーポレーテツド Columns with macroporous polymer media
US5320139A (en) * 1993-03-30 1994-06-14 Millipore Corporation Fluid delivery system
US5603351A (en) * 1995-06-07 1997-02-18 David Sarnoff Research Center, Inc. Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device
US5757482A (en) 1995-04-20 1998-05-26 Perseptive Biosystems, Inc. Module for optical detection in microscale fluidic analyses
AU726987B2 (en) * 1996-06-28 2000-11-30 Caliper Life Sciences, Inc. Electropipettor and compensation means for electrophoretic bias
US6447727B1 (en) * 1996-11-19 2002-09-10 Caliper Technologies Corp. Microfluidic systems
US6379929B1 (en) * 1996-11-20 2002-04-30 The Regents Of The University Of Michigan Chip-based isothermal amplification devices and methods
WO1998049548A1 (en) * 1997-04-25 1998-11-05 Caliper Technologies Corporation Microfluidic devices incorporating improved channel geometries
US6319476B1 (en) 1999-03-02 2001-11-20 Perseptive Biosystems, Inc. Microfluidic connector
US6977145B2 (en) * 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6210986B1 (en) * 1999-09-23 2001-04-03 Sandia Corporation Microfluidic channel fabrication method
US6701774B2 (en) * 2000-08-02 2004-03-09 Symyx Technologies, Inc. Parallel gas chromatograph with microdetector array
WO2002028531A1 (en) * 2000-10-06 2002-04-11 Protasis Corporation Fluid separation conduit cartridge with encryption capability
US20020186263A1 (en) * 2001-06-07 2002-12-12 Nanostream, Inc. Microfluidic fraction collectors
US6919046B2 (en) * 2001-06-07 2005-07-19 Nanostream, Inc. Microfluidic analytical devices and methods
US6581441B1 (en) * 2002-02-01 2003-06-24 Perseptive Biosystems, Inc. Capillary column chromatography process and system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4483773A (en) * 1982-10-04 1984-11-20 Varian Associates, Inc. Narrow bore micro-particulate column packing process and product
US5348658A (en) * 1990-05-29 1994-09-20 Waters Investments Limited Process for effecting capillary electrophoresis
WO2000025128A1 (en) * 1998-10-23 2000-05-04 Battelle Memorial Institute Method and apparatus for packed column separations and purifications
WO2001038865A1 (en) * 1999-11-26 2001-05-31 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems

Also Published As

Publication number Publication date
JP2008139323A (en) 2008-06-19
JP2005517179A (en) 2005-06-09
US6581441B1 (en) 2003-06-24
US6993958B2 (en) 2006-02-07
JP4157476B2 (en) 2008-10-01
US20030200794A1 (en) 2003-10-30
WO2003067249A2 (en) 2003-08-14
EP2851683A3 (en) 2015-07-01
EP1470416B1 (en) 2014-09-24
EP2851683A2 (en) 2015-03-25
EP1470416A2 (en) 2004-10-27

Similar Documents

Publication Publication Date Title
WO2003067249A3 (en) Capillary column chromatography process and system
US8323488B2 (en) IC-processed polymer nano-liquid chromatoraphy system on-a-chip and method of making it
US7182371B1 (en) Edge compression manifold apparatus
US6536477B1 (en) Fluidic couplers and modular microfluidic systems
EP2210089B1 (en) Ion transport apparatuses and methods using a barrier with seated ion exchange bead
US6867857B2 (en) Flow cell for optical analysis of a fluid
US6936167B2 (en) System and method for performing multiple parallel chromatographic separations
CN108490104B (en) Miniature gas chromatographic column chip and preparation method thereof
WO2006015308A2 (en) Modular microfluidic packaging system
WO2005068996A3 (en) Phased micro analyzer iv
WO2000030751A3 (en) Microplate sample and reagent loading system
WO2006113527A3 (en) Integrated chromatography devices and systems for monitoring analytes in real time and methods for manufacturing the same
WO2001009598A1 (en) Fluidic interconnect, interconnect manifold and microfluidic devices for internal delivery of gases and application of vacuum
CN112067533B (en) Single cell mass spectrometry system and method
US20040092033A1 (en) Systems and methods for preparing microfluidic devices for operation
WO2004064974A3 (en) Narrow i.d. monolithic capillary columns for high efficiency separation and high sensitivity analysis of biomolecules
US20240033656A1 (en) Fluidically coupling with elastic structure deformable by sealing element
US8449658B2 (en) High-pressure capillary degas assembly
Fritzsch et al. Pressure-resistant and reversible on-tube-sealing for microfluidics
WO2005043112A3 (en) Apparatus and method for edman degradation on a microfluidic device utilizing an electroosmotic flow pump
CN115518700A (en) Multi-channel micro-fluidic chip for parallel sample introduction
CN115138409A (en) Rotary valve suitable for microfluidic system integration
US20050223821A1 (en) Sampling system
US20030080562A1 (en) Micro fluidic interconnect port system
EP1556687A1 (en) System and method for performing multiple parallel chromatographic separations

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2003566548

Country of ref document: JP

WWE Wipo information: entry into national phase

Ref document number: 2003737548

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2003737548

Country of ref document: EP