WO2003093790A3 - Sensors for biomolecular detection and cell classification - Google Patents

Sensors for biomolecular detection and cell classification Download PDF

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Publication number
WO2003093790A3
WO2003093790A3 PCT/US2003/013538 US0313538W WO03093790A3 WO 2003093790 A3 WO2003093790 A3 WO 2003093790A3 US 0313538 W US0313538 W US 0313538W WO 03093790 A3 WO03093790 A3 WO 03093790A3
Authority
WO
WIPO (PCT)
Prior art keywords
plates
coated
conductive
cell
tissue section
Prior art date
Application number
PCT/US2003/013538
Other languages
French (fr)
Other versions
WO2003093790A2 (en
Inventor
William R Moyle
C Scaduto Russell Jr
Original Assignee
William R Moyle
C Scaduto Russell Jr
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by William R Moyle, C Scaduto Russell Jr filed Critical William R Moyle
Priority to AU2003234315A priority Critical patent/AU2003234315A1/en
Priority to JP2005517161A priority patent/JP4361057B2/en
Priority to AU2003290523A priority patent/AU2003290523B2/en
Priority to EP03783059.3A priority patent/EP1629080B1/en
Priority to PCT/US2003/031486 priority patent/WO2005080546A1/en
Priority to CA2521711A priority patent/CA2521711C/en
Publication of WO2003093790A2 publication Critical patent/WO2003093790A2/en
Publication of WO2003093790A3 publication Critical patent/WO2003093790A3/en
Priority to US10/962,003 priority patent/US20050214789A1/en
Priority to US11/231,654 priority patent/US7384781B2/en
Priority to US12/800,574 priority patent/US20110259744A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis

Abstract

The present invention provides a sensor device for detecting and quantifying a gene product in a cell or tissue section sample (101) by employing an analysis reagent that binds to the gene product to form a detectable product. The device comprises (a) a first and second coated plate (104), wherein the plates (104) are parallel to each other and are coated with a conductive material; (b) a first and second conductive plate (102), wherein the plates are juxtaposed over the coated plates (104); (c) a first and second conducting tape (103) connecting a first and second end of the coated plates (104) and the conductive plates (102), respectively; (d) a first and second gasket insulator (105) insulating a first and second end of the coated plates (104) and the conductive plates (102), respectively; (e) a voltage generator (109) connected to the first and second conductive plates (102) to apply an electric potential to the conductive plates (102); and (f) a detector (108). The first and second coated plates (104) provide a compartment for a cell or tissue section sample (101) and a conductive fluid. An analysis reagent is provided in the sample or tethered to a surface of the first or second coated plate (104) such that when the voltage generator (109) applies an electric potential to the conductive plates (102), the detector (108) will detect the interaction between charged materials within the cell or tissue section sample (101), migrating towards either surface of the coated plate (104), and the analysis reagent.
PCT/US2003/013538 2002-04-30 2003-04-30 Sensors for biomolecular detection and cell classification WO2003093790A2 (en)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AU2003234315A AU2003234315A1 (en) 2002-04-30 2003-04-30 Sensors for biomolecular detection and cell classification
JP2005517161A JP4361057B2 (en) 2003-04-30 2003-10-03 Sensors for biomolecule detection and cell classification
AU2003290523A AU2003290523B2 (en) 2003-04-30 2003-10-03 Sensors for biomolecular detection and cell classification
EP03783059.3A EP1629080B1 (en) 2003-04-30 2003-10-03 Method for biomolecular detection and cell classification
PCT/US2003/031486 WO2005080546A1 (en) 2003-04-30 2003-10-03 Sensors for biomolecular detection and cell classification
CA2521711A CA2521711C (en) 2003-04-30 2003-10-03 Sensors for biomolecular detection and cell classification
US10/962,003 US20050214789A1 (en) 2003-04-30 2004-10-08 Sensors for biomolecular detection and cell classification
US11/231,654 US7384781B2 (en) 2003-04-30 2005-09-21 Sensors for biomolecular detection and cell classification
US12/800,574 US20110259744A1 (en) 2003-04-30 2010-05-18 Sensors for biomolecular detection and cell classification

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37631202P 2002-04-30 2002-04-30
US60/376,312 2002-04-30

Related Child Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/031486 Continuation-In-Part WO2005080546A1 (en) 2003-04-30 2003-10-03 Sensors for biomolecular detection and cell classification

Publications (2)

Publication Number Publication Date
WO2003093790A2 WO2003093790A2 (en) 2003-11-13
WO2003093790A3 true WO2003093790A3 (en) 2004-04-22

Family

ID=29401329

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/013538 WO2003093790A2 (en) 2002-04-30 2003-04-30 Sensors for biomolecular detection and cell classification

Country Status (2)

Country Link
AU (1) AU2003234315A1 (en)
WO (1) WO2003093790A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210048410A1 (en) * 2018-04-30 2021-02-18 The Regents Of The University Of California Tissue Projection Electrophoretic Separation of Protein
CN113368569A (en) * 2021-06-30 2021-09-10 中国核电工程有限公司 Filter unit and pit filter comprising same
CN113368568A (en) * 2021-06-30 2021-09-10 中国核电工程有限公司 Filter unit and pit filter comprising same

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639671A (en) * 1989-09-18 1997-06-17 Biostar, Inc. Methods for optimizing of an optical assay device
US5958704A (en) * 1997-03-12 1999-09-28 Ddx, Inc. Sensing system for specific substance and molecule detection
US6210977B1 (en) * 1996-01-17 2001-04-03 Micronas Intermetall Gmbh Measuring device and method for making same
US6404497B1 (en) * 1999-01-25 2002-06-11 Massachusetts Institute Of Technology Polarized light scattering spectroscopy of tissue

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5639671A (en) * 1989-09-18 1997-06-17 Biostar, Inc. Methods for optimizing of an optical assay device
US6210977B1 (en) * 1996-01-17 2001-04-03 Micronas Intermetall Gmbh Measuring device and method for making same
US5958704A (en) * 1997-03-12 1999-09-28 Ddx, Inc. Sensing system for specific substance and molecule detection
US6404497B1 (en) * 1999-01-25 2002-06-11 Massachusetts Institute Of Technology Polarized light scattering spectroscopy of tissue

Also Published As

Publication number Publication date
AU2003234315A1 (en) 2003-11-17
WO2003093790A2 (en) 2003-11-13
AU2003234315A8 (en) 2003-11-17

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