WO2003104420A3 - Electrochemical detection of single nucleotide polymorphisms (snps) - Google Patents

Electrochemical detection of single nucleotide polymorphisms (snps) Download PDF

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Publication number
WO2003104420A3
WO2003104420A3 PCT/US2003/018093 US0318093W WO03104420A3 WO 2003104420 A3 WO2003104420 A3 WO 2003104420A3 US 0318093 W US0318093 W US 0318093W WO 03104420 A3 WO03104420 A3 WO 03104420A3
Authority
WO
WIPO (PCT)
Prior art keywords
extension reaction
nucleic acid
single nucleotide
primer
snps
Prior art date
Application number
PCT/US2003/018093
Other languages
French (fr)
Other versions
WO2003104420A2 (en
Inventor
Werner G Kuhr
Sara A Brazill
Original Assignee
Univ California
Werner G Kuhr
Sara A Brazill
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Werner G Kuhr, Sara A Brazill filed Critical Univ California
Priority to AU2003251424A priority Critical patent/AU2003251424A1/en
Priority to EP03757435A priority patent/EP1543153A2/en
Publication of WO2003104420A2 publication Critical patent/WO2003104420A2/en
Publication of WO2003104420A3 publication Critical patent/WO2003104420A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Abstract

This invention provides novel methods for the detection of single nucleotide polymorphisms (SNPs). In one embodiment, the method involves i) providing a nucleic acid primer complementary to a nucleic acid sequence adjacent to the location of the single nucleotide comprising said single nucleotide polymorphism wherein said primer is of sufficient length to initiate transcription by a nucleic acid polymerase; ii) performing a primer extension reaction using a nucleic acid polymerase, a chain terminator to produce one or more extension reaction products, wherein one or more components of said primer extension reaction are labeled with a redox-active label whereby one or more extension reaction products produced by the primer extension reaction are labeled with said redoxactive label; iii) detecting the extension reaction products by detecting the redox-active label, wherein detection of an extension reaction product longer than the nucleic acid primer indicates the presence of said single nucleotide polymorphism in said target nucleic acid.
PCT/US2003/018093 2002-06-07 2003-06-06 Electrochemical detection of single nucleotide polymorphisms (snps) WO2003104420A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003251424A AU2003251424A1 (en) 2002-06-07 2003-06-06 Electrochemical detection of single nucleotide polymorphisms (snps)
EP03757435A EP1543153A2 (en) 2002-06-07 2003-06-06 Electrochemical detection of single nucleotide polymorphisms (snps)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16559302A 2002-06-07 2002-06-07
US10/165,593 2002-06-07

Publications (2)

Publication Number Publication Date
WO2003104420A2 WO2003104420A2 (en) 2003-12-18
WO2003104420A3 true WO2003104420A3 (en) 2004-05-13

Family

ID=29732079

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/018093 WO2003104420A2 (en) 2002-06-07 2003-06-06 Electrochemical detection of single nucleotide polymorphisms (snps)

Country Status (3)

Country Link
EP (1) EP1543153A2 (en)
AU (1) AU2003251424A1 (en)
WO (1) WO2003104420A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1682564A1 (en) * 2003-10-27 2006-07-26 Genelabs Technologies, Inc. METHODS FOR PREPARING 7-(2 -SUBSTITUTED-s-D-RIBOFURANO SYL)-4-(NR2R3)-5-(SUBSTITUTED ETHYN-1-YL)-PYRROLO 2,3-D|PYRIMIDINE DERIVATIVES
WO2007077952A1 (en) * 2005-12-28 2007-07-12 Canon Kabushiki Kaisha Method and device for determining a nucleic acid allele or base sequence
EA027558B1 (en) * 2011-05-19 2017-08-31 Эйджена Байосайенс, Инк. Process for multiplex nucleic acid identification
CN104232773A (en) * 2014-06-11 2014-12-24 国家体育总局体育科学研究所 SNP (single nucleotide polymorphism) compound detection system for screening exercise-induced cardiac accidents
CN107406882B (en) 2015-04-24 2022-03-01 基纳生物技术有限公司 Multiplexing method for identification and quantification of minor alleles and polymorphisms

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5608063A (en) * 1986-07-02 1997-03-04 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US6212093B1 (en) * 2000-01-14 2001-04-03 North Carolina State University High-density non-volatile memory devices incorporating sandwich coordination compounds
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6361671B1 (en) * 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5608063A (en) * 1986-07-02 1997-03-04 E. I. Du Pont De Nemours And Company Method, system and reagents for DNA sequencing
US6290839B1 (en) * 1998-06-23 2001-09-18 Clinical Micro Sensors, Inc. Systems for electrophoretic transport and detection of analytes
US6361671B1 (en) * 1999-01-11 2002-03-26 The Regents Of The University Of California Microfabricated capillary electrophoresis chip and method for simultaneously detecting multiple redox labels
US6212093B1 (en) * 2000-01-14 2001-04-03 North Carolina State University High-density non-volatile memory devices incorporating sandwich coordination compounds

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
AHERN H.: "Biochemical, reagent kits offer scientists good return on investment", THE SCIENTIST, vol. 9, no. 15, 24 July 1995 (1995-07-24), pages 1 - 5, XP002921157 *

Also Published As

Publication number Publication date
EP1543153A2 (en) 2005-06-22
AU2003251424A1 (en) 2003-12-22
AU2003251424A8 (en) 2003-12-22
WO2003104420A2 (en) 2003-12-18

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