WO2004011911A3 - Use of dye to distinguish salt and protein crystals under microcrystallization conditions - Google Patents

Use of dye to distinguish salt and protein crystals under microcrystallization conditions Download PDF

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Publication number
WO2004011911A3
WO2004011911A3 PCT/US2003/024083 US0324083W WO2004011911A3 WO 2004011911 A3 WO2004011911 A3 WO 2004011911A3 US 0324083 W US0324083 W US 0324083W WO 2004011911 A3 WO2004011911 A3 WO 2004011911A3
Authority
WO
WIPO (PCT)
Prior art keywords
crystals
crystallization
oil
dyes
conditions
Prior art date
Application number
PCT/US2003/024083
Other languages
French (fr)
Other versions
WO2004011911A2 (en
Inventor
Larry Cosenza
Thomas E Gester
Lawrence J Delucas
Terry L Bray
David T Hamrick
Original Assignee
Uab Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uab Research Foundation filed Critical Uab Research Foundation
Priority to AU2003269927A priority Critical patent/AU2003269927A1/en
Publication of WO2004011911A2 publication Critical patent/WO2004011911A2/en
Publication of WO2004011911A3 publication Critical patent/WO2004011911A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B29/00—Single crystals or homogeneous polycrystalline material with defined structure characterised by the material or by their shape
    • C30B29/54—Organic compounds
    • C30B29/58—Macromolecular compounds
    • C—CHEMISTRY; METALLURGY
    • C30—CRYSTAL GROWTH
    • C30B—SINGLE-CRYSTAL GROWTH; UNIDIRECTIONAL SOLIDIFICATION OF EUTECTIC MATERIAL OR UNIDIRECTIONAL DEMIXING OF EUTECTOID MATERIAL; REFINING BY ZONE-MELTING OF MATERIAL; PRODUCTION OF A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; SINGLE CRYSTALS OR HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; AFTER-TREATMENT OF SINGLE CRYSTALS OR A HOMOGENEOUS POLYCRYSTALLINE MATERIAL WITH DEFINED STRUCTURE; APPARATUS THEREFOR
    • C30B7/00—Single-crystal growth from solutions using solvents which are liquid at normal temperature, e.g. aqueous solutions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6827—Total protein determination, e.g. albumin in urine
    • G01N33/6839—Total protein determination, e.g. albumin in urine involving dyes, e.g. Coomassie blue, bromcresol green
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N25/00—Investigating or analyzing materials by the use of thermal means
    • G01N25/14—Investigating or analyzing materials by the use of thermal means by using distillation, extraction, sublimation, condensation, freezing, or crystallisation
    • G01N25/147—Investigating or analyzing materials by the use of thermal means by using distillation, extraction, sublimation, condensation, freezing, or crystallisation by cristallisation

Abstract

An improved method of screening crystal growth conditions is provided wherein molecules are crystallized from solutions containing dyes. These dyes are selectively incorporated or associated with crystals of particular character thereby rendering crystals of particular character colored and improving detection of the dyed crystals. A preferred method involves use of dyes in protein solutions overlayed by oil. Use of oil allows the use of small volumes of solution and facilitates the screening of large numbers of crystallization conditions in arrays using automated devices that dispense appropriate solutions to generate crystallization trials, overlay crystallization trials with an oil, provide appropriate conditions conducive to crystallization and enhance detection of dyed (colored) or undyed (uncolored) crystals that result.
PCT/US2003/024083 2002-07-30 2003-07-30 Use of dye to distinguish salt and protein crystals under microcrystallization conditions WO2004011911A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003269927A AU2003269927A1 (en) 2002-07-30 2003-07-30 Use of dye to distinguish salt and protein crystals under microcrystallization conditions

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/208,576 2002-07-30
US10/208,576 US7250305B2 (en) 2001-07-30 2002-07-30 Use of dye to distinguish salt and protein crystals under microcrystallization conditions

Publications (2)

Publication Number Publication Date
WO2004011911A2 WO2004011911A2 (en) 2004-02-05
WO2004011911A3 true WO2004011911A3 (en) 2004-06-10

Family

ID=31186849

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/024083 WO2004011911A2 (en) 2002-07-30 2003-07-30 Use of dye to distinguish salt and protein crystals under microcrystallization conditions

Country Status (3)

Country Link
US (2) US7250305B2 (en)
AU (1) AU2003269927A1 (en)
WO (1) WO2004011911A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7244396B2 (en) * 1999-04-06 2007-07-17 Uab Research Foundation Method for preparation of microarrays for screening of crystal growth conditions
US7214540B2 (en) 1999-04-06 2007-05-08 Uab Research Foundation Method for screening crystallization conditions in solution crystal growth
US7250305B2 (en) * 2001-07-30 2007-07-31 Uab Research Foundation Use of dye to distinguish salt and protein crystals under microcrystallization conditions
US7670429B2 (en) 2001-04-05 2010-03-02 The California Institute Of Technology High throughput screening of crystallization of materials
JP3759891B2 (en) * 2001-09-27 2006-03-29 独立行政法人理化学研究所 Protein crystallization method and apparatus
WO2008102469A1 (en) * 2007-02-23 2008-08-28 Kwansei Gakuin Educational Foundation Protein crystallizing agent and method of crystallizing protein therewith
JP5040597B2 (en) * 2007-11-06 2012-10-03 日本電気株式会社 Evaluation system, evaluation method, and evaluation program
CN109001199A (en) * 2018-06-06 2018-12-14 西北工业大学 A method of differentiating salt crystalline substance and protein crystal

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6296673B1 (en) * 1999-06-18 2001-10-02 The Regents Of The University Of California Methods and apparatus for performing array microcrystallizations

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Also Published As

Publication number Publication date
AU2003269927A8 (en) 2004-02-16
WO2004011911A2 (en) 2004-02-05
US20070111317A1 (en) 2007-05-17
AU2003269927A1 (en) 2004-02-16
US7250305B2 (en) 2007-07-31
US20030180960A1 (en) 2003-09-25

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