WO2004024886A3 - Microarray synthesis and assembly of gene-length polynucleotides - Google Patents
Microarray synthesis and assembly of gene-length polynucleotides Download PDFInfo
- Publication number
- WO2004024886A3 WO2004024886A3 PCT/US2003/028946 US0328946W WO2004024886A3 WO 2004024886 A3 WO2004024886 A3 WO 2004024886A3 US 0328946 W US0328946 W US 0328946W WO 2004024886 A3 WO2004024886 A3 WO 2004024886A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- assembly
- gene
- length polynucleotides
- synthesis
- microarray synthesis
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/14—Solid phase synthesis, i.e. wherein one or more library building blocks are bound to a solid support during library creation; Particular methods of cleavage from the solid support
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B80/00—Linkers or spacers specially adapted for combinatorial chemistry or libraries, e.g. traceless linkers or safety-catch linkers
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00452—Means for the recovery of reactants or products
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- B01J2219/00603—Making arrays on substantially continuous surfaces
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- B01J2219/00623—Immobilisation or binding
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- B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
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- B01J2219/00709—Type of synthesis
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- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
Abstract
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2004536347A JP4783874B2 (en) | 2002-09-12 | 2003-09-12 | Microarray synthesis and assembly of gene-length polynucleotides |
EP15189572.9A EP3059321B1 (en) | 2002-09-12 | 2003-09-12 | Microarray synthesis and assembly of gene-length polynucleotides |
EP03752373.5A EP1546387B1 (en) | 2002-09-12 | 2003-09-12 | Microarray synthesis and assembly of gene-length polynucleotides |
EP20189157.9A EP3770270A1 (en) | 2002-09-12 | 2003-09-12 | Microassay synthesis and assembly of gene-length polynucleotides |
CA2498746A CA2498746C (en) | 2002-09-12 | 2003-09-12 | Microarray synthesis and assembly of gene-length polynucleotides |
AU2003270666A AU2003270666B2 (en) | 2002-09-12 | 2003-09-12 | Microarray synthesis and assembly of gene-length polynucleotides |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/243,367 US7563600B2 (en) | 2002-09-12 | 2002-09-12 | Microarray synthesis and assembly of gene-length polynucleotides |
US10/243,367 | 2002-09-12 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2004024886A2 WO2004024886A2 (en) | 2004-03-25 |
WO2004024886A3 true WO2004024886A3 (en) | 2005-01-20 |
Family
ID=31991621
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/028946 WO2004024886A2 (en) | 2002-09-12 | 2003-09-12 | Microarray synthesis and assembly of gene-length polynucleotides |
Country Status (7)
Country | Link |
---|---|
US (9) | US7563600B2 (en) |
EP (4) | EP3770270A1 (en) |
JP (1) | JP4783874B2 (en) |
AU (1) | AU2003270666B2 (en) |
CA (2) | CA3020587C (en) |
DK (1) | DK3059321T3 (en) |
WO (1) | WO2004024886A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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US9216414B2 (en) | 2009-11-25 | 2015-12-22 | Gen9, Inc. | Microfluidic devices and methods for gene synthesis |
US9217144B2 (en) | 2010-01-07 | 2015-12-22 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
Families Citing this family (92)
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US10539561B1 (en) | 2001-08-30 | 2020-01-21 | Customarray, Inc. | Enzyme-amplified redox microarray detection process |
EP1483409B1 (en) * | 2002-02-28 | 2009-04-15 | Wisconsin Alumni Research Foundation | Method of error reduction in nucleic acid populations |
US7563600B2 (en) | 2002-09-12 | 2009-07-21 | Combimatrix Corporation | Microarray synthesis and assembly of gene-length polynucleotides |
WO2005089110A2 (en) * | 2004-02-27 | 2005-09-29 | President And Fellows Of Harvard College | Polynucleotide synthesis |
JP2008502367A (en) * | 2004-06-09 | 2008-01-31 | ウイスコンシン アラムニ リサーチ ファンデーション | Fast generation of oligonucleotides |
JP2008523786A (en) * | 2004-10-18 | 2008-07-10 | コドン デバイシズ インコーポレイテッド | Method for assembling high fidelity synthetic polynucleotides |
US20060194214A1 (en) * | 2005-02-28 | 2006-08-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
US20070122817A1 (en) * | 2005-02-28 | 2007-05-31 | George Church | Methods for assembly of high fidelity synthetic polynucleotides |
US20060102471A1 (en) | 2004-11-18 | 2006-05-18 | Karl Maurer | Electrode array device having an adsorbed porous reaction layer |
US20070034513A1 (en) | 2005-03-25 | 2007-02-15 | Combimatrix Corporation | Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array |
US9394167B2 (en) | 2005-04-15 | 2016-07-19 | Customarray, Inc. | Neutralization and containment of redox species produced by circumferential electrodes |
US20070065877A1 (en) | 2005-09-19 | 2007-03-22 | Combimatrix Corporation | Microarray having a base cleavable succinate linker |
US20070087417A1 (en) * | 2005-10-14 | 2007-04-19 | Eugeni Namsaraev | Multiplex polynucleotide synthesis |
WO2007111937A1 (en) | 2006-03-23 | 2007-10-04 | Applera Corporation | Directed enrichment of genomic dna for high-throughput sequencing |
US20070231805A1 (en) * | 2006-03-31 | 2007-10-04 | Baynes Brian M | Nucleic acid assembly optimization using clamped mismatch binding proteins |
US20070259346A1 (en) * | 2006-05-03 | 2007-11-08 | Agilent Technologies, Inc. | Analysis of arrays |
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US9068209B2 (en) | 2008-05-14 | 2015-06-30 | British Columbia Cancer Agency Branch | Gene synthesis by convergent assembly of oligonucleotide subsets |
US20100047876A1 (en) * | 2008-08-08 | 2010-02-25 | President And Fellows Of Harvard College | Hierarchical assembly of polynucleotides |
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US9927434B2 (en) | 2010-01-20 | 2018-03-27 | Customarray, Inc. | Multiplex microarray of serially deposited biomolecules on a microarray |
US8716467B2 (en) | 2010-03-03 | 2014-05-06 | Gen9, Inc. | Methods and devices for nucleic acid synthesis |
US9187777B2 (en) | 2010-05-28 | 2015-11-17 | Gen9, Inc. | Methods and devices for in situ nucleic acid synthesis |
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US9873900B2 (en) | 2012-02-10 | 2018-01-23 | Wisconsin Alumni Research Foundation | RNA-mediated gene assembly from DNA oligonucleotides |
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