WO2004026804A8 - Anthraquinone quencher dyes, their methods of preparation and use - Google Patents

Anthraquinone quencher dyes, their methods of preparation and use

Info

Publication number
WO2004026804A8
WO2004026804A8 PCT/US2003/029324 US0329324W WO2004026804A8 WO 2004026804 A8 WO2004026804 A8 WO 2004026804A8 US 0329324 W US0329324 W US 0329324W WO 2004026804 A8 WO2004026804 A8 WO 2004026804A8
Authority
WO
WIPO (PCT)
Prior art keywords
anthraquinone
methods
quenchers
preparation
quencher dyes
Prior art date
Application number
PCT/US2003/029324
Other languages
French (fr)
Other versions
WO2004026804A1 (en
Inventor
Mark Aaron Behlke
Andrei Laikhter
Lingyan Huang
Scott Rose
Yawfui Yong
Original Assignee
Integrated Dna Tech Inc
Mark Aaron Behlke
Andrei Laikhter
Lingyan Huang
Scott Rose
Yawfui Yong
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Integrated Dna Tech Inc, Mark Aaron Behlke, Andrei Laikhter, Lingyan Huang, Scott Rose, Yawfui Yong filed Critical Integrated Dna Tech Inc
Priority to CA002498320A priority Critical patent/CA2498320A1/en
Priority to EP03759288A priority patent/EP1556325A4/en
Priority to JP2004537958A priority patent/JP4694201B2/en
Priority to AU2003275018A priority patent/AU2003275018B2/en
Publication of WO2004026804A1 publication Critical patent/WO2004026804A1/en
Publication of WO2004026804A8 publication Critical patent/WO2004026804A8/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C225/00—Compounds containing amino groups and doubly—bound oxygen atoms bound to the same carbon skeleton, at least one of the doubly—bound oxygen atoms not being part of a —CHO group, e.g. amino ketones
    • C07C225/24—Compounds containing amino groups and doubly—bound oxygen atoms bound to the same carbon skeleton, at least one of the doubly—bound oxygen atoms not being part of a —CHO group, e.g. amino ketones the carbon skeleton containing carbon atoms of quinone rings
    • C07C225/26—Compounds containing amino groups and doubly—bound oxygen atoms bound to the same carbon skeleton, at least one of the doubly—bound oxygen atoms not being part of a —CHO group, e.g. amino ketones the carbon skeleton containing carbon atoms of quinone rings having amino groups bound to carbon atoms of quinone rings or of condensed ring systems containing quinone rings
    • C07C225/32—Compounds containing amino groups and doubly—bound oxygen atoms bound to the same carbon skeleton, at least one of the doubly—bound oxygen atoms not being part of a —CHO group, e.g. amino ketones the carbon skeleton containing carbon atoms of quinone rings having amino groups bound to carbon atoms of quinone rings or of condensed ring systems containing quinone rings of condensed quinone ring systems formed by at least three rings
    • C07C225/34—Amino anthraquinones
    • C07C225/36—Amino anthraquinones the carbon skeleton being further substituted by singly-bound oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/08—Esters of oxyacids of phosphorus
    • C07F9/09—Esters of phosphoric acids
    • C07F9/091—Esters of phosphoric acids with hydroxyalkyl compounds with further substituents on alkyl
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic System
    • C07F9/02—Phosphorus compounds
    • C07F9/06—Phosphorus compounds without P—C bonds
    • C07F9/22—Amides of acids of phosphorus
    • C07F9/24—Esteramides
    • C07F9/2404—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/2408—Esteramides the ester moiety containing a substituent or a structure which is considered as characteristic of hydroxyalkyl compounds
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B1/00—Dyes with anthracene nucleus not condensed with any other ring
    • C09B1/16—Amino-anthraquinones
    • C09B1/20—Preparation from starting materials already containing the anthracene nucleus
    • C09B1/26—Dyes with amino groups substituted by hydrocarbon radicals
    • C09B1/32—Dyes with amino groups substituted by hydrocarbon radicals substituted by aryl groups
    • C09B1/325—Dyes with no other substituents than the amino groups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

The invention provides novel anthraquinone compositions that are useful as broad-spectrum quenchers of fluorescence and provides methods for making and using them. The anthraquinone quenchers can be conjugated to a variety of biologically relevant compounds, including lipids, nucleic acids, polypeptides, and more specifically antigens, steroids, vitamins, drugs, haptens, metabolites, toxins, environmental pollutants, amino acids, peptides, proteins, nucleotides, oligonucleotides, polynucleotides, carbohydrates, and their analogs. The invention also provides kits comprising, in one or more containers, at least one anthraquinone quencher dye composition of the present invention, and instructions for using that composition.
PCT/US2003/029324 2002-09-20 2003-09-19 Anthraquinone quencher dyes, their methods of preparation and use WO2004026804A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002498320A CA2498320A1 (en) 2002-09-20 2003-09-19 Anthraquinone quencher dyes, their methods of preparation and use
EP03759288A EP1556325A4 (en) 2002-09-20 2003-09-19 Anthraquinone quencher dyes, their methods of preparation and use
JP2004537958A JP4694201B2 (en) 2002-09-20 2003-09-19 Anthraquinone quenching dyes, their production and use
AU2003275018A AU2003275018B2 (en) 2002-09-20 2003-09-19 Anthraquinone quencher dyes, their methods of preparation and use

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US41221502P 2002-09-20 2002-09-20
US60/412,215 2002-09-20

Publications (2)

Publication Number Publication Date
WO2004026804A1 WO2004026804A1 (en) 2004-04-01
WO2004026804A8 true WO2004026804A8 (en) 2005-12-01

Family

ID=32030830

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/029324 WO2004026804A1 (en) 2002-09-20 2003-09-19 Anthraquinone quencher dyes, their methods of preparation and use

Country Status (6)

Country Link
US (2) US7803536B2 (en)
EP (2) EP1556325A4 (en)
JP (1) JP4694201B2 (en)
AU (1) AU2003275018B2 (en)
CA (1) CA2498320A1 (en)
WO (1) WO2004026804A1 (en)

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US9506059B2 (en) 2010-03-26 2016-11-29 Integrated Dna Technologies, Inc. Modifications for antisense compounds
US9540515B2 (en) 2003-11-14 2017-01-10 Integrated Dna Technologies, Inc. Fluorescence quenching azo dyes, their methods of preparation and use

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US8114979B2 (en) 2012-02-14
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WO2004026804A1 (en) 2004-04-01
EP1556325A1 (en) 2005-07-27
JP2006500408A (en) 2006-01-05
US20040110308A1 (en) 2004-06-10
AU2003275018B2 (en) 2009-10-01
EP2281798A1 (en) 2011-02-09
AU2003275018A1 (en) 2004-04-08
US20110060150A1 (en) 2011-03-10
EP1556325A4 (en) 2007-09-19
US7803536B2 (en) 2010-09-28

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