WO2004035102A1 - Method of replacing human soft tissue volume by in vitro culturing human preadipocyte in injectable material - Google Patents
Method of replacing human soft tissue volume by in vitro culturing human preadipocyte in injectable material Download PDFInfo
- Publication number
- WO2004035102A1 WO2004035102A1 PCT/KR2003/002150 KR0302150W WO2004035102A1 WO 2004035102 A1 WO2004035102 A1 WO 2004035102A1 KR 0302150 W KR0302150 W KR 0302150W WO 2004035102 A1 WO2004035102 A1 WO 2004035102A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- human
- soft tissue
- cell
- replacing
- tissue volume
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/04—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
- A61L27/3847—Bones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
- A61L27/3852—Cartilage, e.g. meniscus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/60—Materials for use in artificial skin
Definitions
- the prevent invention is related to a method of replacing human soft tissue volume, more particularly to a method comprising the steps of; in vitro culturing patient's preadipocytes in particles which have a fine bubble structure and can pass through a syringe needle; then transplanting them as an injectable material to a portion of human body.
- the present invention has been made in view of the above problems, and it is an object of the present invention to provide to a method of replacing the human soft tissue volume comprising steps of; in vitro culturing patient's preadipocytes in particles having a fine bubble structure which size is sufficient to pass through a syringe needle; then injecting and transplanting them as an injectable material to the human body.
- the said material is as a transplantable solid material having sponge structure and each particle size of 20-250jum; mixing them with a liquid as a culture fluid, a serum, a saline solution; injecting and transplanting them as an injectable material to the human body.
- the precursor cells can overcome the demerit of incision scar on the donor site, since they can be obtained from small amount of fat extracted from portion of axilla, circumference of umbilicus, hip with a 1cm of small incision and then cultured.
- Sponge structure is very useful structure in cell culture and used in general cell culture.
- each particle size is limited from
- chitosan sponge powder polyethylene, PTFT particle(polytetrafluoethylene), PLGA particle(DL-lactic-co-glycolic acid) as an applicable material which is the said sponge structure of solid material.
- These solid materials are not only three-dimensional cell culture scaffolds but also cell carriers.
- the culture was performed under condition of in vitro culture.
- the cultured cells were injected into a cell scaffold then became an injectable state. Thereafter they are mixed with the liquid such as the culture fluid, the serum, the saline solution.
- Chitosan is a structure formed by repeating basic structures called N- acetylglucoseamine. Its efficacy has been disclosed such as biocompatibility, biodegradability, hemostatic activity, capability for wound healing, thereby it is used as a drugcarrier, an anti-cancer medicine, a hemostatic, a wound healing material in bio medicine.
- This sponge having fine bubble structure has appropriate property as a cell scaffold due to ease to control bio-decomposition and its affinity for cell.
- polyethylene Medpore produced by POREX Company is a porous polyethylene and used as a cartilage bone or a fungible for bone in the shape of board or block. It is manufactured into fine powder and can be used as a substitute for the said chitosan sponge powder.
- PTFT particle(polytetrafluoroethyelene) is used mainly as a surgical artificial blood vessel.
- Expanded PTFT manufactured by Gore Company is composed of micro-sized fine holes. It can be used also by inserting into the body in the shape of board or block, which has soft tissue or blood vessel grow in the said fine holes. Also it is manufactured into fine powder and can be used as a substitute for the said chitosan sponge powder.
- PLA PLGA particle (DL-lactic-co-glycolic acid) is a mixture used as an insertion to human body. It is manufactured and also used in the shape of board having sponge structure. Also it is manufactured into fine powder and can be used as substitution for the said chitosan sponge powder.
- the said solid material which is not only a culture ground(a culture material) but also a cell carrier is transplantable material for the human body.
- the process of culturing in the solid sponge powder then injecting offers the most advantageous terms in point of the culture material providing short-term volume effects, culture easiness and high survival probability.
- a method is devised sufficiently without using the said solid scaffold as a culture ground and a carrier according to circumstances. This method is to extract preadipocyte; and to cut it finely after cell culture or to cut it finely without culture process; then to inject directly for the purpose of replacing human soft tissue.
- the said method is exceptional, it makes use of transplanting in vitro cultured preadipocyte of which state is not solid particle but plane or three dimension for replacing the human soft tissue volume. It can be useful valuably to obtain rather long-term effect than short-term volume effect, which is soft tissue has increased as a result a preadipocyte specialized into a fat cell.
- the present invention enables to remove limitation of extraction quantity by in vitro culturing the preadipocytes. And also by transplanting the said preadipocytes, it enables to augment volume rather than to minimize volume decrease effect.
- preadipocyte, chondroblast, osteoblast as human cells applicable to this invention.
- Preadipocyte is known as a previous step cell of a fat cell, it becomes a fat cell through growth and specialization.
- Fat cell has been exhausted in process of fat transplantation, actually survivor is a fat cell specialized from a preadipocyte, thus it is the most important cell when transplanting.
- preadipocyte it can function as a mother cell specializing into a bone cell or a chondrocyte. Thereby the said preadipocyte is getting more important.
- Chondroblast has a feature that it grows and specializes into chondrocyte. It has some problems as following. There are many portions which need it in human body, but its obtainable quantity is limited. And in order not to cause complication of donor site, it is required to extract only small quantity from the donor site.
- Osteoblast is a mother cell creating bone in human body. It is possible to obtain large quantity of bone by culturing and multiplying small amount of it from the donor site then injecting into a cell scaffold thereafter injecting the said scaffold into the human body.
- the present invention as mentioned above is a method of transplanting the cultured cell through injection for the purpose of maintaining the volume, it can maximize cell viability by transplanting the cells with a culture ground itself.
- its volume augmentation effect is insufficient, thus there is a problem to replace the human soft tissue volume.
- the present invention provides the most effective method of volume augmentation by injecting three-dimensionally cultured cell into the human body.
- the present invention offers a method as the best for the volume augmentation, that is to culture not a fat cell but a preadipocyte then to inject it into a three-dimensional space, in other words a scaffold in which it can grow and specialize, thereafter to transplant the said scaffold to human body.
- This scaffold form is also obtained as result of many researches, in which sponge structure having a fine bubble structure is used. And it can supply the most proper nutrition to the cell which needs to grow and to specialize.
- the present invention provides one method as the simplest form, that is to extract preadipocyte; and to culture it or without culture process; and then to cut it finely; thereafter to transplant directly it as an injectable material for the purpose of replacing the human soft tissue volume.
- the present invention also provides another novel method of replacing the human soft tissue volume, the said method comprising the stages of; using a scaffold such as chitosan sponge powder, polyethylene, PTFT particle(polytetrafluoroethyelene), PLGA particle(DL-lactic-co-glygolic acid); making form of the said scaffold a fine bubble structure which is injectable through a thin syringe needle; herein injecting distributively the cultured preadipocytes into the said scaffold; thereafter injecting an injectable material along with the said scaffold having a fine bubble structure and the said preadipocytes.
- a scaffold such as chitosan sponge powder, polyethylene, PTFT particle(polytetrafluoroethyelene), PLGA particle(DL-lactic-co-glygolic acid
- one of the objects of the present invention is accomplished by injecting and transplanting not the state of solid particle but in vitro cultured preadipocytes in plane or in three-dimension for the purpose of replacing the human soft tissue volume, it is rather to attain long-term effect resulted from volume augment after preadipocytes specialized into fat cells than to attain short-term volume effect.
- object of the present invention that is to create various cosmetic effects, also accomplished by various experiment for the volume augmentation effect and then by calculating exactly ratio of the said volume augmentation after transplanting preadipocytes or chondrocytes to the human body. Accordingly it is feasible to supplement the incision scar the biggest shortcoming in view of existing cosmetic effects, to perform easily an operation, and to achieve economical surgical operation.
Abstract
Description
Claims
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2003269552A AU2003269552A1 (en) | 2002-10-21 | 2003-10-15 | Method of replacing human soft tissue volume by in vitro culturing human preadipocyte in injectable material |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR10-2002-0064189A KR100539371B1 (en) | 2002-10-21 | 2002-10-21 | In vitro cultured human preadipocyte for human soft tissue volume replacement with injectable material as a scaffold |
KR10-2002-0064189 | 2002-10-21 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2004035102A1 true WO2004035102A1 (en) | 2004-04-29 |
Family
ID=27728891
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/KR2003/002150 WO2004035102A1 (en) | 2002-10-21 | 2003-10-15 | Method of replacing human soft tissue volume by in vitro culturing human preadipocyte in injectable material |
Country Status (3)
Country | Link |
---|---|
KR (1) | KR100539371B1 (en) |
AU (1) | AU2003269552A1 (en) |
WO (1) | WO2004035102A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2436837A (en) * | 2006-04-05 | 2007-10-10 | Univ Open | Culturing adipocytes |
CN105530967A (en) * | 2013-07-18 | 2016-04-27 | 柳胜皓 | Body volume substitute comprising cartilage for grafting by injection, and dual needle-type syringe for injecting same |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR101713475B1 (en) | 2016-01-29 | 2017-03-07 | 류승호 | Injectable tissue volume replacement material comprising cartilage |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5716404A (en) * | 1994-12-16 | 1998-02-10 | Massachusetts Institute Of Technology | Breast tissue engineering |
US5855610A (en) * | 1995-05-19 | 1999-01-05 | Children's Medical Center Corporation | Engineering of strong, pliable tissues |
US6124273A (en) * | 1995-06-09 | 2000-09-26 | Chitogenics, Inc. | Chitin hydrogels, methods of their production and use |
US20020076400A1 (en) * | 1999-03-10 | 2002-06-20 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Adipose-derived stem cells and lattices |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5906934A (en) * | 1995-03-14 | 1999-05-25 | Morphogen Pharmaceuticals, Inc. | Mesenchymal stem cells for cartilage repair |
US5700289A (en) * | 1995-10-20 | 1997-12-23 | North Shore University Hospital Research Corporation | Tissue-engineered bone repair using cultured periosteal cells |
IL118376A0 (en) * | 1996-05-22 | 1996-09-12 | Univ Ben Gurion | Polysaccharide sponges for cell culture and transplantation |
US5919702A (en) * | 1996-10-23 | 1999-07-06 | Advanced Tissue Science, Inc. | Production of cartilage tissue using cells isolated from Wharton's jelly |
US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
-
2002
- 2002-10-21 KR KR10-2002-0064189A patent/KR100539371B1/en active IP Right Grant
-
2003
- 2003-10-15 AU AU2003269552A patent/AU2003269552A1/en not_active Abandoned
- 2003-10-15 WO PCT/KR2003/002150 patent/WO2004035102A1/en not_active Application Discontinuation
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5716404A (en) * | 1994-12-16 | 1998-02-10 | Massachusetts Institute Of Technology | Breast tissue engineering |
US5855610A (en) * | 1995-05-19 | 1999-01-05 | Children's Medical Center Corporation | Engineering of strong, pliable tissues |
US6124273A (en) * | 1995-06-09 | 2000-09-26 | Chitogenics, Inc. | Chitin hydrogels, methods of their production and use |
US20020076400A1 (en) * | 1999-03-10 | 2002-06-20 | University Of Pittsburgh Of The Commonwealth System Of Higher Education | Adipose-derived stem cells and lattices |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2436837A (en) * | 2006-04-05 | 2007-10-10 | Univ Open | Culturing adipocytes |
CN105530967A (en) * | 2013-07-18 | 2016-04-27 | 柳胜皓 | Body volume substitute comprising cartilage for grafting by injection, and dual needle-type syringe for injecting same |
Also Published As
Publication number | Publication date |
---|---|
KR100539371B1 (en) | 2005-12-27 |
AU2003269552A1 (en) | 2004-05-04 |
KR20020084056A (en) | 2002-11-04 |
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