WO2004046687A3 - Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates - Google Patents

Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates Download PDF

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Publication number
WO2004046687A3
WO2004046687A3 PCT/US2003/036153 US0336153W WO2004046687A3 WO 2004046687 A3 WO2004046687 A3 WO 2004046687A3 US 0336153 W US0336153 W US 0336153W WO 2004046687 A3 WO2004046687 A3 WO 2004046687A3
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WO
WIPO (PCT)
Prior art keywords
complexes
methods
nucleic acid
bioconjugates
biosensing
Prior art date
Application number
PCT/US2003/036153
Other languages
French (fr)
Other versions
WO2004046687A2 (en
Inventor
David G Whitten
Robert M Jones
Stuart A Kushon
Kevin D Ley
Wensheng Xia
Sriram Kumaraswamy
Duncan W Mcbranch
Original Assignee
Qtl Biosystems Llc
David G Whitten
Robert M Jones
Stuart A Kushon
Kevin D Ley
Wensheng Xia
Sriram Kumaraswamy
Duncan W Mcbranch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qtl Biosystems Llc, David G Whitten, Robert M Jones, Stuart A Kushon, Kevin D Ley, Wensheng Xia, Sriram Kumaraswamy, Duncan W Mcbranch filed Critical Qtl Biosystems Llc
Priority to EP03786675A priority Critical patent/EP1579215A4/en
Priority to AU2003295485A priority patent/AU2003295485A1/en
Priority to CA002505907A priority patent/CA2505907A1/en
Priority to JP2004553594A priority patent/JP2006506643A/en
Publication of WO2004046687A2 publication Critical patent/WO2004046687A2/en
Publication of WO2004046687A3 publication Critical patent/WO2004046687A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82B—NANOSTRUCTURES FORMED BY MANIPULATION OF INDIVIDUAL ATOMS, MOLECULES, OR LIMITED COLLECTIONS OF ATOMS OR MOLECULES AS DISCRETE UNITS; MANUFACTURE OR TREATMENT THEREOF
    • B82B1/00—Nanostructures formed by manipulation of individual atoms or molecules, or limited collections of atoms or molecules as discrete units
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors

Abstract

Complexes of a biotinylated fluorescent polymer and a biotin binding protein and solid supports coated with the fluorescent polymer complexes are described. The complexes can be used as sensors for detecting biological recognition events (e.g., nucleic acid hybridization reactions or enzymatic induced polypeptide cleavage). Methods of making the complexes and methods of using the complexes for detecting the presence and/or amount of a target analyte in a sample are also described. The target analyte can be an enzyme (e.g., β-secretase) or a nucleic acid (e.g., a single stranded or double stranded nucleic acid).
PCT/US2003/036153 2002-11-14 2003-11-14 Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates WO2004046687A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03786675A EP1579215A4 (en) 2002-11-14 2003-11-14 Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates
AU2003295485A AU2003295485A1 (en) 2002-11-14 2003-11-14 Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates
CA002505907A CA2505907A1 (en) 2002-11-14 2003-11-14 Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates
JP2004553594A JP2006506643A (en) 2002-11-14 2003-11-14 Biosensing method using fluorescent polymer and quencher-linking chain-ligand bioconjugate

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42603402P 2002-11-14 2002-11-14
US60/426,034 2002-11-14

Publications (2)

Publication Number Publication Date
WO2004046687A2 WO2004046687A2 (en) 2004-06-03
WO2004046687A3 true WO2004046687A3 (en) 2005-07-21

Family

ID=32326307

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/036153 WO2004046687A2 (en) 2002-11-14 2003-11-14 Methods of biosensing using fluorescent polymers and quencher-tether-ligand bioconjugates

Country Status (8)

Country Link
US (1) US20040175768A1 (en)
EP (1) EP1579215A4 (en)
JP (1) JP2006506643A (en)
KR (1) KR20050086658A (en)
CN (1) CN1742201A (en)
AU (1) AU2003295485A1 (en)
CA (1) CA2505907A1 (en)
WO (1) WO2004046687A2 (en)

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US20050147534A1 (en) * 1998-05-05 2005-07-07 Massachusetts Institute Of Technology Emissive sensors and devices incorporating these sensors
US6706474B1 (en) 2000-06-27 2004-03-16 Board Of Trustees Of The University Of Illinois Nucleic acid enzyme biosensors for ions
US7462325B2 (en) * 2001-11-30 2008-12-09 Nomadics, Inc. Luminescent polymer particles
US6890719B2 (en) 2002-05-10 2005-05-10 The Board Of Trustess Of The University Of Illinois Fluorescence based biosensor
US20050014160A1 (en) * 2003-07-18 2005-01-20 Sriram Kumaraswamy Assays for protease enzyme activity
US8617819B2 (en) 2004-09-17 2013-12-31 Massachusetts Institute Of Technology Polymers for analyte detection
US20070026388A1 (en) * 2005-07-28 2007-02-01 Doorn Stephen K Analyte detection using carbon nanotubes
US7892734B2 (en) 2005-08-11 2011-02-22 The Board Of Trustees Of The University Of Illinois Aptamer based colorimetric sensor systems
WO2007109500A1 (en) 2006-03-16 2007-09-27 The Board Of Trustees Of The University Of Illinois Lateral flow devices
US8158437B2 (en) * 2006-08-04 2012-04-17 Massachusetts Institute Of Technology Luminescent detection of hydrazine and hydrazine derivatives
US8283423B2 (en) 2006-09-29 2012-10-09 Massachusetts Institute Of Technology Polymer synthetic technique
US8802447B2 (en) 2006-10-05 2014-08-12 Massachusetts Institute Of Technology Emissive compositions with internal standard and related techniques
US20090215189A1 (en) * 2006-10-27 2009-08-27 Massachusetts Institute Of Technology Sensor of species including toxins and chemical warfare agents
US8058415B2 (en) 2007-04-24 2011-11-15 The Board Of Trustees Of The University Of Illinois Aptamer- and nucleic acid enzyme-based systems for simultaneous detection of multiple analytes
CA2726598C (en) 2008-06-02 2017-05-09 Soo-Kwan Lee Controllable assembly and disassembly of nanoparticle systems via protein and dna agents
DK2318838T3 (en) * 2008-07-16 2013-10-14 Radiometer Medical Aps Fixed phase with high capacity
CN102516836B (en) * 2011-12-03 2014-04-16 东南大学 Application of thiofuran-co-oxadiazole as oxygen quenching substance
GB2502306A (en) * 2012-05-22 2013-11-27 Univ Singapore Microparticle sensor
CN104718455B (en) * 2012-09-07 2017-03-08 基诺麦因有限公司 The detection method of kidney blood biomarker (Biomarker) and kit
EP3172569A1 (en) 2014-07-25 2017-05-31 Selux Diagnostics Inc. Assay methods involving dissociable nanoparticles
KR20190012278A (en) * 2015-03-11 2019-02-08 투 포어 가이즈, 인코포레이티드 Nanopore detection of small molecules through competition assays
JP2018519796A (en) 2015-04-29 2018-07-26 ドッツ テクノロジー コーポレイションＤＯＴＳ Ｔｅｃｈｎｏｌｏｇｙ Ｃｏｒｐ． Compositions and methods for allergen detection
US10844228B2 (en) 2018-03-30 2020-11-24 Becton, Dickinson And Company Water-soluble polymeric dyes having pendant chromophores
CN112771365A (en) 2018-06-28 2021-05-07 贝克顿·迪金森公司 Integrated pre-amplification light detection system and use method thereof
CN111850103B (en) * 2020-08-20 2022-12-02 清华大学深圳国际研究生院 Method for detecting target nucleic acid based on cationic conjugated polymer and nuclease-assisted cyclic amplification
CN113358612B (en) * 2021-05-24 2022-11-08 宁波大学 Micro-nano optical sensor for algae detection and manufacturing and detection method thereof

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US6537829B1 (en) * 1992-09-14 2003-03-25 Sri International Up-converting reporters for biological and other assays using laser excitation techniques

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US6372445B1 (en) * 1997-10-03 2002-04-16 Becton Dickinson And Company Chromophores in the preparation of novel tandem conjugates
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See also references of EP1579215A4 *

Also Published As

Publication number Publication date
KR20050086658A (en) 2005-08-30
JP2006506643A (en) 2006-02-23
EP1579215A2 (en) 2005-09-28
US20040175768A1 (en) 2004-09-09
EP1579215A4 (en) 2007-01-17
CA2505907A1 (en) 2004-06-03
AU2003295485A1 (en) 2004-06-15
CN1742201A (en) 2006-03-01
WO2004046687A2 (en) 2004-06-03

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