WO2004053078A3 - METHODS FOR MODULATING IKKα ACTIVITY - Google Patents

METHODS FOR MODULATING IKKα ACTIVITY Download PDF

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Publication number
WO2004053078A3
WO2004053078A3 PCT/US2003/038852 US0338852W WO2004053078A3 WO 2004053078 A3 WO2004053078 A3 WO 2004053078A3 US 0338852 W US0338852 W US 0338852W WO 2004053078 A3 WO2004053078 A3 WO 2004053078A3
Authority
WO
WIPO (PCT)
Prior art keywords
modulating
methods
ikkα activity
activity
modulating ikkα
Prior art date
Application number
PCT/US2003/038852
Other languages
French (fr)
Other versions
WO2004053078A2 (en
Inventor
Jun Li
Xiang Li
Jianfei Yang
Kenneth Marcu
Original Assignee
Boehringer Ingelheim Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma filed Critical Boehringer Ingelheim Pharma
Priority to AU2003297700A priority Critical patent/AU2003297700A1/en
Publication of WO2004053078A2 publication Critical patent/WO2004053078A2/en
Publication of WO2004053078A3 publication Critical patent/WO2004053078A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Abstract

A method for modulating NF-κB dependent gene transcription in a cell comprised of modulating IKKα protein activity in the cell. The present invention also provides siRNA compositions and methods thereof for modulating NF-κB dependent gene transcription.
PCT/US2003/038852 2002-12-09 2003-12-08 METHODS FOR MODULATING IKKα ACTIVITY WO2004053078A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003297700A AU2003297700A1 (en) 2002-12-09 2003-12-08 METHODS FOR MODULATING IKKAlpha ACTIVITY

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43182502P 2002-12-09 2002-12-09
US60/431,825 2002-12-09

Publications (2)

Publication Number Publication Date
WO2004053078A2 WO2004053078A2 (en) 2004-06-24
WO2004053078A3 true WO2004053078A3 (en) 2005-07-07

Family

ID=32507801

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/038852 WO2004053078A2 (en) 2002-12-09 2003-12-08 METHODS FOR MODULATING IKKα ACTIVITY

Country Status (3)

Country Link
US (3) US7235654B2 (en)
AU (1) AU2003297700A1 (en)
WO (1) WO2004053078A2 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2485184A1 (en) * 2002-05-24 2003-12-04 Boehringer Ingelheim Pharmaceuticals, Inc. Methods for the identification of ikkalpha function and other genes useful for treatment of inflammatory diseases
AU2003297700A1 (en) * 2002-12-09 2004-06-30 Boehringer Ingelheim Pharmaceuticals, Inc. METHODS FOR MODULATING IKKAlpha ACTIVITY
EP1789447B1 (en) 2004-08-16 2012-04-25 Immune Disease Institute, Inc. Method of delivering rna interference and uses thereof
US20060073120A1 (en) * 2004-09-30 2006-04-06 Boehringer Ingelheim Pharmaceuticals, Inc. IKKalpha and IKKbeta specific inhibitors
US8183221B2 (en) * 2007-09-05 2012-05-22 Medtronic, Inc. Suppression of SCN9A gene expression and/or function for the treatment of pain
EP2042592A1 (en) * 2007-09-28 2009-04-01 IMBA-Institut für Molekulare Biotechnologie GmbH Methods for modulating the proliferation and differentiation potential of stem cells and progenitor cells
EP2268620A4 (en) * 2008-03-27 2011-08-10 Burnham Inst Medical Research Inhibitors of antigen receptor-induced nf- kappa b activation
WO2010000903A2 (en) 2008-06-30 2010-01-07 Centro De Investigaciones Energéticas, Medioambientales Y Tecnológicas (Ciemat) Composition capable of modulating ikkalpha activity for the treatment of diseases associated with acantholysis
US8691971B2 (en) 2008-09-23 2014-04-08 Scott G. Petersen Self delivering bio-labile phosphate protected pro-oligos for oligonucleotide based therapeutics and mediating RNA interference
WO2011109427A2 (en) 2010-03-01 2011-09-09 Alnylam Pharmaceuticals, Inc. Improving the biological activity of sirna through modulation of its thermodynamic profile
US20120310140A1 (en) 2010-12-01 2012-12-06 Spinal Modulation, Inc. Directed delivery of agents to neural anatomy
EP2599859A1 (en) 2011-11-30 2013-06-05 IMBA-Institut für Molekulare Biotechnologie GmbH Haploid cells

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US4745119A (en) 1982-10-14 1988-05-17 Burroughs Wellcome Co. Method for using purine derivatives
US5972674A (en) 1996-08-26 1999-10-26 Signal Pharmaceuticals, Inc. Stimulus-inducible protein kinase complex and methods of use therefor
US6235512B1 (en) 1997-07-01 2001-05-22 Tularik Inc. IKK-α proteins, nucleic acids and methods
US5962673A (en) 1998-11-20 1999-10-05 Isis Pharmaceuticals Inc. Antisense modulation of inhibitor-kappa B kinase-alpha expression
US20020037538A1 (en) * 2000-05-09 2002-03-28 Trepicchio William L. Compositions, kits, and methods for identification, assessment, prevention, and therapy of psoriasis
US20030124726A1 (en) 2000-08-03 2003-07-03 Defougerolles Antonin Global expression analysis of extracellular matrix-integrin interactions in monocytes
US20020132788A1 (en) 2000-11-06 2002-09-19 David Lewis Inhibition of gene expression by delivery of small interfering RNA to post-embryonic animal cells in vivo
US20040214167A9 (en) 2000-12-28 2004-10-28 Akio Matsuda NF-kappa B activating gene
US20030170719A1 (en) 2000-12-28 2003-09-11 Akio Matsuda NF-kappa B activating gene
AU2002245255A1 (en) * 2001-01-12 2002-07-24 California Institute Of Technology Pasadena, Ca 91125 Regulation of target protein activity through modified proteins
WO2002074242A2 (en) 2001-03-16 2002-09-26 Tyler Curiel Inhibition of toxoplasma gondii) replication by pyridinylimidazoles
WO2003022987A2 (en) * 2001-07-26 2003-03-20 Eos Biotechnology, Inc. Methods of diagnosis of hepatitis c infection, compositions and methods of screening for modulators of hepatitis c infection
CA2485184A1 (en) 2002-05-24 2003-12-04 Boehringer Ingelheim Pharmaceuticals, Inc. Methods for the identification of ikkalpha function and other genes useful for treatment of inflammatory diseases
AU2003297700A1 (en) * 2002-12-09 2004-06-30 Boehringer Ingelheim Pharmaceuticals, Inc. METHODS FOR MODULATING IKKAlpha ACTIVITY
US20060073120A1 (en) * 2004-09-30 2006-04-06 Boehringer Ingelheim Pharmaceuticals, Inc. IKKalpha and IKKbeta specific inhibitors

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
AGRAWAL ET AL.: "Antisense therapeutics: is it as simple as complementary base recognition?", MOLECULAR MEDICINE TODAY, vol. 6, 2000, pages 72 - 81, XP000979511, DOI: doi:10.1016/S1357-4310(99)01638-X *
BHULLAR ET AL.: "Fluid chear stress activation of IkappaB kinase is integrin-dependent", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, 1998, pages 30544 - 30549, XP002201716, DOI: doi:10.1074/jbc.273.46.30544 *
MICKEY ET AL.: "IkappaB kinase-alpha and -beta genes are coexpressed in adult and embryonic tissues but localized to different human chromosomes", GENE, vol. 222, 1998, pages 31 - 40, XP004151163, DOI: doi:10.1016/S0378-1119(98)00462-4 *
O'MAHONY ET AL.: "Activation of the heterodimeric IkappaB kinase alpha(IKKalpha)-IKKbeta complex is directional: IKKalpha regulates IKKbeta under both nbasal and stimulated conditions", MOLECULAR AND CELLULAR BIOLOGY, vol. 20, 2000, pages 1170 - 1178, XP002213033, DOI: doi:10.1128/MCB.20.4.1170-1178.2000 *
THOMPSON J.D.: "Applications of antisense and siRNAs during preclinical drug development", DRUG DISCOVERY TODAY, vol. 17, 2002, pages 912 - 917, XP002236964, DOI: doi:10.1016/S1359-6446(02)02410-8 *

Also Published As

Publication number Publication date
US20050043257A1 (en) 2005-02-24
AU2003297700A8 (en) 2004-06-30
AU2003297700A1 (en) 2004-06-30
US8088915B2 (en) 2012-01-03
US7235654B2 (en) 2007-06-26
US20070191300A1 (en) 2007-08-16
US20110112284A1 (en) 2011-05-12
US7838268B2 (en) 2010-11-23
WO2004053078A2 (en) 2004-06-24

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