WO2004056870A3 - Method for separation of antithrombin - Google Patents

Method for separation of antithrombin Download PDF

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Publication number
WO2004056870A3
WO2004056870A3 PCT/EP2003/014571 EP0314571W WO2004056870A3 WO 2004056870 A3 WO2004056870 A3 WO 2004056870A3 EP 0314571 W EP0314571 W EP 0314571W WO 2004056870 A3 WO2004056870 A3 WO 2004056870A3
Authority
WO
WIPO (PCT)
Prior art keywords
antithrombin
latent
native
elution
concentration
Prior art date
Application number
PCT/EP2003/014571
Other languages
French (fr)
Other versions
WO2004056870A2 (en
Inventor
Goeran Karlsson
Stefan Winge
Original Assignee
Octapharma Ag
Goeran Karlsson
Stefan Winge
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Octapharma Ag, Goeran Karlsson, Stefan Winge filed Critical Octapharma Ag
Priority to AU2003290096A priority Critical patent/AU2003290096A1/en
Publication of WO2004056870A2 publication Critical patent/WO2004056870A2/en
Publication of WO2004056870A3 publication Critical patent/WO2004056870A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8128—Antithrombin III

Abstract

A method for separation of native antithrombin and pre-latent antithrombin present in a sample from each other is provided. The method comprises performing, in the order given, the following steps: a) application of a sample containing native antithrombin and pre-latent antithrombin to an affinity material with affinity for antithrombin, wherein said application is performed under conditions such that binding of native antithrombin and pre-latent antithrombin to the affinity material is achieved; b) application of a solution of an elution salt S1 to said affinity material at a concentration C1 of elution salt, for elution of pre-latent antithrombin, said concentration C1 is in the range of 30-95 % of the concentration needed for elution of native antithrombin with the elution salt S1. In the method, the concentration C1 is such that pre-latent antithrombin is eluted and native antithrombin is retained.
PCT/EP2003/014571 2002-12-19 2003-12-18 Method for separation of antithrombin WO2004056870A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003290096A AU2003290096A1 (en) 2002-12-19 2003-12-18 Method for separation of antithrombin

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0203770A SE0203770D0 (en) 2002-12-19 2002-12-19 Method of separation
SE0203770-3 2002-12-19

Publications (2)

Publication Number Publication Date
WO2004056870A2 WO2004056870A2 (en) 2004-07-08
WO2004056870A3 true WO2004056870A3 (en) 2004-10-14

Family

ID=20289922

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2003/014571 WO2004056870A2 (en) 2002-12-19 2003-12-18 Method for separation of antithrombin

Country Status (3)

Country Link
AU (1) AU2003290096A1 (en)
SE (1) SE0203770D0 (en)
WO (1) WO2004056870A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3842061A (en) * 1971-09-08 1974-10-15 Kabi Ab Method for isolation of antithrombin from animal tissue materials by adsorption on sulfated carbohydrate gel
US4087415A (en) * 1976-06-09 1978-05-02 William L. Wilson Antithrombin III
JPS5810522A (en) * 1981-07-13 1983-01-21 Nippon Sekijiyuujishiya Purification of antithrombin 3 by deactivated thrombin gel
JPH01275600A (en) * 1988-04-28 1989-11-06 Green Cross Corp:The Method for purifying antithrombin-iii
WO2002038610A1 (en) * 2000-11-08 2002-05-16 Octapharma Ag Process for the preparation of latent antithrombin iii
WO2002060925A1 (en) * 2001-01-30 2002-08-08 Genzymes Corporation Method of producing latent antithrombin iii

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3842061A (en) * 1971-09-08 1974-10-15 Kabi Ab Method for isolation of antithrombin from animal tissue materials by adsorption on sulfated carbohydrate gel
US4087415A (en) * 1976-06-09 1978-05-02 William L. Wilson Antithrombin III
JPS5810522A (en) * 1981-07-13 1983-01-21 Nippon Sekijiyuujishiya Purification of antithrombin 3 by deactivated thrombin gel
JPH01275600A (en) * 1988-04-28 1989-11-06 Green Cross Corp:The Method for purifying antithrombin-iii
WO2002038610A1 (en) * 2000-11-08 2002-05-16 Octapharma Ag Process for the preparation of latent antithrombin iii
WO2002060925A1 (en) * 2001-01-30 2002-08-08 Genzymes Corporation Method of producing latent antithrombin iii

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
ANDREA HEGER ET AL: "Separation of active and inactive forms of human antithrombin by heparin affinity chromatogrphy", THROMBOSIS RESEARCH, vol. 106, 2002, pages 157 - 164, XP002284705 *
GÖRAN KARLSSON ET AL: "Preparative Conversion of native human antithrombin to the latent form", PROTEIN EXPRESSION AND PURIFICATION, vol. 26, 2002, pages 106 - 110, XP002284704 *
GÖRAN KARLSSON ET AL: "Separation of Native and Latent Forms of Human ANtithrombin by Hydrophobic Interaction High Performance Liquid Chromatography", PROTEIN EXPRESSION AND PURIFICATION, vol. 21, 2001, pages 149 - 155, XP002284703 *
HELENA LARSSON ET AL: "A Novel Anti-angiogenic Form of Antithrombin with Retained Proteinase Binding Ability and Heparin Affinity", THE JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 276, no. 15, 2001, pages 11996 - 12002, XP002284702 *
HELENA LARSSON ET AL: "Antiangiogenic Effects of Latent Antithrombin through Perturbed Cell-Matrix Interactions and Apoptosis of Endothelial Cells", CANCER RESEARCH, vol. 60, 2000, pages 6723 - 6729, XP002284706 *
PATENT ABSTRACTS OF JAPAN vol. 007, no. 083 (C - 160) 6 April 1983 (1983-04-06) *
PATENT ABSTRACTS OF JAPAN vol. 014, no. 043 (C - 0681) 26 January 1990 (1990-01-26) *

Also Published As

Publication number Publication date
WO2004056870A2 (en) 2004-07-08
AU2003290096A8 (en) 2004-07-14
AU2003290096A1 (en) 2004-07-14
SE0203770D0 (en) 2002-12-19

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