WO2004067765A3 - Organism fingerprinting using nicking agents - Google Patents

Organism fingerprinting using nicking agents Download PDF

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Publication number
WO2004067765A3
WO2004067765A3 PCT/US2004/002720 US2004002720W WO2004067765A3 WO 2004067765 A3 WO2004067765 A3 WO 2004067765A3 US 2004002720 W US2004002720 W US 2004002720W WO 2004067765 A3 WO2004067765 A3 WO 2004067765A3
Authority
WO
WIPO (PCT)
Prior art keywords
sample
nicking agents
analyzed
nucleic acid
portions
Prior art date
Application number
PCT/US2004/002720
Other languages
French (fr)
Other versions
WO2004067765A2 (en
Inventor
Ness Jeffrey Van
David J Galas
Ness Lori K Van
Original Assignee
Keck Graduate Inst
Ness Jeffrey Van
David J Galas
Ness Lori K Van
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Keck Graduate Inst, Ness Jeffrey Van, David J Galas, Ness Lori K Van filed Critical Keck Graduate Inst
Publication of WO2004067765A2 publication Critical patent/WO2004067765A2/en
Publication of WO2004067765A3 publication Critical patent/WO2004067765A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Abstract

A sample of nucleic acid that is characteristic of a particular source, e.g., a bacterium, can be analyzed to determine whether that particular sample is from a particular source. The analysis protocol subjects the sample to a nicking reaction to produce a family of nicked fragments. In certain embodiments, the nicking reaction is performed in the presence of a polymerase and nucleoside triphosphates to amplify nicked fragments or portions thereof. The nicked fragments or portions thereof may be analyzed to determine the source of the nucleic acid sample.
PCT/US2004/002720 2003-01-29 2004-01-29 Organism fingerprinting using nicking agents WO2004067765A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US44381103P 2003-01-29 2003-01-29
US60/443,811 2003-01-29

Publications (2)

Publication Number Publication Date
WO2004067765A2 WO2004067765A2 (en) 2004-08-12
WO2004067765A3 true WO2004067765A3 (en) 2006-09-28

Family

ID=32825374

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/002720 WO2004067765A2 (en) 2003-01-29 2004-01-29 Organism fingerprinting using nicking agents

Country Status (1)

Country Link
WO (1) WO2004067765A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4822801B2 (en) 2005-10-24 2011-11-24 西川ゴム工業株式会社 Mutant endonuclease
WO2012077819A1 (en) * 2010-12-10 2012-06-14 国立大学法人 東京工業大学 Method and kit for detecting target nucleic acid
US9410156B2 (en) 2012-03-28 2016-08-09 Somalogic, Inc. Aptamers to PDGF and VEGF and their use in treating PDGF and VEGF mediated conditions
EP3693472A1 (en) 2013-09-09 2020-08-12 Somalogic, Inc. Pdgf and vegf aptamers having improved stability and their use in treating pdgf and vegf mediated diseases and disorders
US10036077B2 (en) 2014-01-15 2018-07-31 Abbott Laboratories Covered sequence conversion DNA and detection methods
WO2016059473A2 (en) 2014-10-14 2016-04-21 Abbott Japan Co., Ltd Sequence conversion and signal amplifier dna having locked nucleic acids and detection methods using same
WO2016103234A1 (en) 2014-12-24 2016-06-30 Abbott Japan Co., Ltd. Detection method based on signal amplifier dna cascade reactions with target extension

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5874215A (en) * 1995-01-16 1999-02-23 Keygene N.V. Amplification of simple sequence repeats

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5874215A (en) * 1995-01-16 1999-02-23 Keygene N.V. Amplification of simple sequence repeats
US5614386A (en) * 1995-06-23 1997-03-25 Baylor College Of Medicine Alternative dye-labeled primers for automated DNA sequencing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
COLLINS ET AL.: "DNA Fingerprinting of Mycobacterium bovis Strains by Restriction Fragment Analysis and Hybridization with Insertion Elements IS1081 and IS6110", J. OF CLINICAL MICROBIOLOGY, vol. 31, no. 5, May 1993 (1993-05-01), pages 1143 - 1147, XP003001112 *
SIEGERT ET AL.: "Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for the Detection of Polymerase Chain Reaction Products containing 7-Deazapurine Moieties", ANALYICAL BIOCHEMISTRY, vol. 243, 1996, pages 55 - 65, XP002072977 *

Also Published As

Publication number Publication date
WO2004067765A2 (en) 2004-08-12

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