WO2004081528A3 - Assay device and method - Google Patents

Assay device and method Download PDF

Info

Publication number
WO2004081528A3
WO2004081528A3 PCT/US2004/007231 US2004007231W WO2004081528A3 WO 2004081528 A3 WO2004081528 A3 WO 2004081528A3 US 2004007231 W US2004007231 W US 2004007231W WO 2004081528 A3 WO2004081528 A3 WO 2004081528A3
Authority
WO
WIPO (PCT)
Prior art keywords
reagent
labeled
binding
specific
test device
Prior art date
Application number
PCT/US2004/007231
Other languages
French (fr)
Other versions
WO2004081528A2 (en
Inventor
Joseph R Robinson
Benedict L Zin
Original Assignee
Joseph R Robinson
Benedict L Zin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Joseph R Robinson, Benedict L Zin filed Critical Joseph R Robinson
Publication of WO2004081528A2 publication Critical patent/WO2004081528A2/en
Publication of WO2004081528A3 publication Critical patent/WO2004081528A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G01N33/54387—Immunochromatographic test strips
    • G01N33/54388—Immunochromatographic test strips based on lateral flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors

Abstract

The present invention provides a test device for detecting the presence or absence of a selected analyte in a liquid sample. The test device includes a reagent member, a porous carrier, and a detection zone. The reagent member includes a body, a first labeled binding reagent specific for a first binding site of the analyte and a second labeled binding reagent specific for a second binding site of the analyte. The first and second specific binding sites are different. The reagent body is adapted to retain the first and second labeled specific binding reagents when the body and the first and second labeled binding reagent are dry, and to release them when the body and they are moist. The detection zones includes a first porous barrier having an average pore size larger than the diameter of the larger diameter of the first or second labeled specific binding reagent, but smaller than the diameter of the first labeled complex. The reagent member, porous carrier, and detection zone are arranged so that a fluid applied to the test device would travel sequentially from the reagent member to the porous carrier and to the detection zone.
PCT/US2004/007231 2003-03-10 2004-03-09 Assay device and method WO2004081528A2 (en)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US45502903P 2003-03-10 2003-03-10
US60/455,029 2003-03-10
US45374103P 2003-03-11 2003-03-11
US60/453,741 2003-03-11
US45397703P 2003-03-12 2003-03-12
US60/453,977 2003-03-12

Publications (2)

Publication Number Publication Date
WO2004081528A2 WO2004081528A2 (en) 2004-09-23
WO2004081528A3 true WO2004081528A3 (en) 2006-09-08

Family

ID=32995988

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/007231 WO2004081528A2 (en) 2003-03-10 2004-03-09 Assay device and method

Country Status (1)

Country Link
WO (1) WO2004081528A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103314297B (en) * 2010-09-30 2015-10-14 积水医疗株式会社 Immune chromatograph testing strip and manufacture method thereof
CN110850081B (en) 2014-03-07 2024-02-06 加利福尼亚大学董事会 Device for integrated analyte extraction, concentration and detection
WO2017041030A1 (en) 2015-09-04 2017-03-09 The Regents Of The University Of California Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications
EP3469365B1 (en) 2016-06-09 2022-12-07 The Regents of the University of California Biomarker concentration and signal amplification for use in paper-based immunoassays
WO2018039139A1 (en) 2016-08-22 2018-03-01 The Regents Of The University Of California Hydrogel platform for aqueous two-phase concentration of a target to enhance its detection
US11209427B2 (en) 2017-03-27 2021-12-28 The Regents Of The University Of California Semi-quantitative lateral-flow immunoassay for the detection of CSF leaks

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824268A (en) * 1995-05-19 1998-10-20 Universal Health Watch, Inc. Rapid self-contained assay format
US6472226B1 (en) * 1998-10-02 2002-10-29 Genosis Limited Assay using porosity-reduction to inhibit migration
US6753189B1 (en) * 1998-06-04 2004-06-22 Mizuho Medy Co., Ltd. Detection apparatus and method for the same
US7026002B1 (en) * 1999-06-18 2006-04-11 Roche Diagnostics Gmbh Element, method and kit for the determination of an analyte in a liquid

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824268A (en) * 1995-05-19 1998-10-20 Universal Health Watch, Inc. Rapid self-contained assay format
US6753189B1 (en) * 1998-06-04 2004-06-22 Mizuho Medy Co., Ltd. Detection apparatus and method for the same
US6472226B1 (en) * 1998-10-02 2002-10-29 Genosis Limited Assay using porosity-reduction to inhibit migration
US7026002B1 (en) * 1999-06-18 2006-04-11 Roche Diagnostics Gmbh Element, method and kit for the determination of an analyte in a liquid

Also Published As

Publication number Publication date
WO2004081528A2 (en) 2004-09-23

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