WO2004101735A3 - Method and device for culturing tissue - Google Patents

Method and device for culturing tissue Download PDF

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Publication number
WO2004101735A3
WO2004101735A3 PCT/GB2004/001821 GB2004001821W WO2004101735A3 WO 2004101735 A3 WO2004101735 A3 WO 2004101735A3 GB 2004001821 W GB2004001821 W GB 2004001821W WO 2004101735 A3 WO2004101735 A3 WO 2004101735A3
Authority
WO
WIPO (PCT)
Prior art keywords
scaffold
vascular endothelial
tissue
cells
endothelial cells
Prior art date
Application number
PCT/GB2004/001821
Other languages
French (fr)
Other versions
WO2004101735A2 (en
Inventor
John Michael Garland
Original Assignee
Univ Manchester
John Michael Garland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Manchester, John Michael Garland filed Critical Univ Manchester
Publication of WO2004101735A2 publication Critical patent/WO2004101735A2/en
Publication of WO2004101735A3 publication Critical patent/WO2004101735A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/28—Vascular endothelial cells

Abstract

A method and device for culturing tissue, in particular microvascularized tissue, to aid tissue graft survival by establishing a network of small vessels that can be linked to the host arterial and venous supplies. A scaffold (2) seeded with the cells destined to be the tissue is provided with an upper guide layer (4) having a pattern of apertures (6) therethrough and vascular endothelial cells (5) are delivered to the layer. The scaffold is provided with a chemoattractant for the vascular endothelial cells, such as vascular endothelial growth factor, and fluid is passed over the guide layer. The apertures allow fluid and vascular endothelial cells to migrate into the scaffold only at these focal points to generate capillary sprouts, the migration of cells being stimulated by the chemoattractant within the scaffold.
PCT/GB2004/001821 2003-05-13 2004-04-28 Method and device for culturing tissue WO2004101735A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0310925A GB2401612A (en) 2003-05-13 2003-05-13 Method and device for culturing tissue
GB0310925.3 2003-05-13

Publications (2)

Publication Number Publication Date
WO2004101735A2 WO2004101735A2 (en) 2004-11-25
WO2004101735A3 true WO2004101735A3 (en) 2005-02-03

Family

ID=9957939

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2004/001821 WO2004101735A2 (en) 2003-05-13 2004-04-28 Method and device for culturing tissue

Country Status (2)

Country Link
GB (1) GB2401612A (en)
WO (1) WO2004101735A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006088029A1 (en) * 2005-02-15 2006-08-24 School Juridical Person Kitasato Gakuen Method of producing high-density cultured tissue and high-density cultured tissue
US9051550B2 (en) 2009-04-09 2015-06-09 Arizona Board Of Regents, On Behalf Of The University Of Arizona Cellular seeding and co-culture of a three dimensional fibroblast construct
ES2357125B1 (en) * 2009-10-05 2012-03-14 Universidad De Zaragoza C�? MARA FOR CELL CULTURE ON ANDAMIAJES.
AU2018283372A1 (en) 2017-06-16 2020-02-06 Avery Therapeutics, Inc. Three dimensional tissue compositions and methods of use

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066036A2 (en) * 1999-04-30 2000-11-09 Massachusetts General Hospital Fabrication of vascularized tissue using microfabricated two-dimensional molds
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
US20020197239A1 (en) * 1997-09-25 2002-12-26 Pykett Mark J. Methods and devices for the long-culture of hematopoietic progenitor cells

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9808836D0 (en) * 1998-04-27 1998-06-24 Amersham Pharm Biotech Uk Ltd Microfabricated apparatus for cell based assays
JP4222658B2 (en) * 1998-06-23 2009-02-12 テルモ株式会社 Cell support substrate, culture apparatus and liquid processing apparatus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020197239A1 (en) * 1997-09-25 2002-12-26 Pykett Mark J. Methods and devices for the long-culture of hematopoietic progenitor cells
US6197575B1 (en) * 1998-03-18 2001-03-06 Massachusetts Institute Of Technology Vascularized perfused microtissue/micro-organ arrays
WO2000066036A2 (en) * 1999-04-30 2000-11-09 Massachusetts General Hospital Fabrication of vascularized tissue using microfabricated two-dimensional molds

Also Published As

Publication number Publication date
GB0310925D0 (en) 2003-06-18
GB2401612A (en) 2004-11-17
WO2004101735A2 (en) 2004-11-25

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