WO2004101749A3 - Methods for amplification of nucleic acid sequences using staggered ligation - Google Patents

Methods for amplification of nucleic acid sequences using staggered ligation Download PDF

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Publication number
WO2004101749A3
WO2004101749A3 PCT/US2004/014325 US2004014325W WO2004101749A3 WO 2004101749 A3 WO2004101749 A3 WO 2004101749A3 US 2004014325 W US2004014325 W US 2004014325W WO 2004101749 A3 WO2004101749 A3 WO 2004101749A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
nucleic acid
amplification
acid sequences
ligation
Prior art date
Application number
PCT/US2004/014325
Other languages
French (fr)
Other versions
WO2004101749A2 (en
Inventor
Robert C Getts
Jaime Schwalm
Original Assignee
Genisphere Inc
Robert C Getts
Jaime Schwalm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genisphere Inc, Robert C Getts, Jaime Schwalm filed Critical Genisphere Inc
Priority to US10/554,374 priority Critical patent/US7494789B2/en
Priority to DE602004022282T priority patent/DE602004022282D1/en
Priority to AT04751627T priority patent/ATE437964T1/en
Priority to EP04751627A priority patent/EP1623044B1/en
Priority to JP2006532858A priority patent/JP4599483B2/en
Priority to AU2004239283A priority patent/AU2004239283B2/en
Publication of WO2004101749A2 publication Critical patent/WO2004101749A2/en
Publication of WO2004101749A3 publication Critical patent/WO2004101749A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

Methods and kits are provided for producing sense RNA molecules. The sense RNA molecules can be used in various research and diagnostic applications, such as gene expression studies involving nucleic acid microarrays.
PCT/US2004/014325 2003-05-09 2004-05-07 Methods for amplification of nucleic acid sequences using staggered ligation WO2004101749A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
US10/554,374 US7494789B2 (en) 2003-05-09 2004-05-07 Methods for amplification of nucleic acid sequences using staggered ligation
DE602004022282T DE602004022282D1 (en) 2003-05-09 2004-05-07 METHOD FOR THE AMPLIFICATION OF NUCLEIC ACID SEQUENCES BY MEANS OF TRIED LIGATION
AT04751627T ATE437964T1 (en) 2003-05-09 2004-05-07 METHOD FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES USING STAGED LIGATION
EP04751627A EP1623044B1 (en) 2003-05-09 2004-05-07 Methods for amplification of nucleic acid sequences using staggered ligation
JP2006532858A JP4599483B2 (en) 2003-05-09 2004-05-07 Method for amplifying nucleic acid sequences using staggered ligation
AU2004239283A AU2004239283B2 (en) 2003-05-09 2004-05-07 Methods for amplification of nucleic acid sequences using staggered ligation

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46938303P 2003-05-09 2003-05-09
US60/469,383 2003-05-09

Publications (2)

Publication Number Publication Date
WO2004101749A2 WO2004101749A2 (en) 2004-11-25
WO2004101749A3 true WO2004101749A3 (en) 2005-02-10

Family

ID=33452281

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/014325 WO2004101749A2 (en) 2003-05-09 2004-05-07 Methods for amplification of nucleic acid sequences using staggered ligation

Country Status (7)

Country Link
US (1) US7494789B2 (en)
EP (1) EP1623044B1 (en)
JP (1) JP4599483B2 (en)
AT (1) ATE437964T1 (en)
AU (1) AU2004239283B2 (en)
DE (1) DE602004022282D1 (en)
WO (1) WO2004101749A2 (en)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602005017252D1 (en) * 2004-04-01 2009-12-03 Genisphere Inc PROCESS FOR THE AMPLIFICATION OF NUCLEIC ACIDS BY PROMOTER TEMPLATES
US7550264B2 (en) 2005-06-10 2009-06-23 Datascope Investment Corporation Methods and kits for sense RNA synthesis
US20070105124A1 (en) 2005-11-08 2007-05-10 Getts Robert C Methods and kits for nucleic acid amplification
US7998423B2 (en) 2007-02-27 2011-08-16 Basf Corporation SCR on low thermal mass filter substrates
AU2009321519A1 (en) * 2008-11-03 2011-06-30 Alchemy Biosciences Pty Ltd Method of cloning DNA
JP6509723B2 (en) * 2012-03-13 2019-05-08 スウィフト バイオサイエンシーズ， インコーポレイテッド Methods and compositions for size-controlled homopolymeric tailing of substrate polynucleotides by nucleic acid polymerase
WO2014108850A2 (en) * 2013-01-09 2014-07-17 Yeda Research And Development Co. Ltd. High throughput transcriptome analysis
GB201903391D0 (en) * 2019-03-12 2019-04-24 Cancer Research Tech Ltd Nucleic acid amplification methods

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US6338954B1 (en) * 1998-02-27 2002-01-15 Pamgene B.V. Method for the non-specific amplification of nucleic acid

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020102589A1 (en) 2000-10-13 2002-08-01 Ryoiti Kiyama Microarrays and methods for evaluating activity of compounds having estrogen-like activity
JP2002360249A (en) * 2000-10-13 2002-12-17 National Institute Of Advanced Industrial & Technology Microarray and method for evaluating activity of compound having estrogen-like activity
US20030104432A1 (en) * 2001-07-27 2003-06-05 The Regents Of The University Of California Methods of amplifying sense strand RNA
GB0123401D0 (en) * 2001-09-28 2001-11-21 Novartis Forschungsstiftung Methods of inducing gene expression
DE602005017252D1 (en) 2004-04-01 2009-12-03 Genisphere Inc PROCESS FOR THE AMPLIFICATION OF NUCLEIC ACIDS BY PROMOTER TEMPLATES

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5194370A (en) * 1990-05-16 1993-03-16 Life Technologies, Inc. Promoter ligation activated transcription amplification of nucleic acid sequences
US6338954B1 (en) * 1998-02-27 2002-01-15 Pamgene B.V. Method for the non-specific amplification of nucleic acid

Also Published As

Publication number Publication date
DE602004022282D1 (en) 2009-09-10
ATE437964T1 (en) 2009-08-15
EP1623044A4 (en) 2007-11-07
AU2004239283A1 (en) 2004-11-25
JP2007515938A (en) 2007-06-21
US7494789B2 (en) 2009-02-24
AU2004239283B2 (en) 2010-01-28
WO2004101749A2 (en) 2004-11-25
EP1623044A2 (en) 2006-02-08
JP4599483B2 (en) 2010-12-15
US20070072182A1 (en) 2007-03-29
EP1623044B1 (en) 2009-07-29

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