WO2005019801A3 - Method for rapid identification of molecules with functional activity towards biomolecular targets - Google Patents

Method for rapid identification of molecules with functional activity towards biomolecular targets Download PDF

Info

Publication number
WO2005019801A3
WO2005019801A3 PCT/US2004/027125 US2004027125W WO2005019801A3 WO 2005019801 A3 WO2005019801 A3 WO 2005019801A3 US 2004027125 W US2004027125 W US 2004027125W WO 2005019801 A3 WO2005019801 A3 WO 2005019801A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
biomolecular targets
biomolecular
functional activity
activity towards
Prior art date
Application number
PCT/US2004/027125
Other languages
French (fr)
Other versions
WO2005019801A2 (en
Inventor
Steven A Hofstadler
Lendell L Cummins
Kristin A Sannes-Lowery
Original Assignee
Isis Pharmaceuticals Inc
Steven A Hofstadler
Lendell L Cummins
Kristin A Sannes-Lowery
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals Inc, Steven A Hofstadler, Lendell L Cummins, Kristin A Sannes-Lowery filed Critical Isis Pharmaceuticals Inc
Publication of WO2005019801A2 publication Critical patent/WO2005019801A2/en
Publication of WO2005019801A3 publication Critical patent/WO2005019801A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry

Abstract

The present invention provides mass spectrometry-based methods for high throughput identification of molecules with functional activities such as nuclease, protease, reductase, kinase, phosphatase, or tranferase activities towards biomolecular targets. These methods are useful for screening biological samples such as extracts, broths, lysates and natural product mixtures and provide valuable insights into biomolecular interactions of various biological ligands with biomolecular targets.
PCT/US2004/027125 2003-08-20 2004-08-20 Method for rapid identification of molecules with functional activity towards biomolecular targets WO2005019801A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US49695103P 2003-08-20 2003-08-20
US60/496,951 2003-08-20

Publications (2)

Publication Number Publication Date
WO2005019801A2 WO2005019801A2 (en) 2005-03-03
WO2005019801A3 true WO2005019801A3 (en) 2005-10-13

Family

ID=34216055

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/027125 WO2005019801A2 (en) 2003-08-20 2004-08-20 Method for rapid identification of molecules with functional activity towards biomolecular targets

Country Status (2)

Country Link
US (2) US20050095622A1 (en)
WO (1) WO2005019801A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9598724B2 (en) 2007-06-01 2017-03-21 Ibis Biosciences, Inc. Methods and compositions for multiple displacement amplification of nucleic acids
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051378A (en) * 1996-03-04 2000-04-18 Genetrace Systems Inc. Methods of screening nucleic acids using mass spectrometry
US6453244B1 (en) * 2000-02-10 2002-09-17 Stanford University Detection of polymorphisms by denaturing high-performance liquid chromatography
US6486309B1 (en) * 2001-08-01 2002-11-26 Varian, Inc. Method of detecting sequence variations in macromolecules by thermal gradiant

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2502018A1 (en) * 1981-03-20 1982-09-24 Philips Ind Commerciale RETURN ARRAY RETURN ARM
US6428956B1 (en) * 1998-03-02 2002-08-06 Isis Pharmaceuticals, Inc. Mass spectrometric methods for biomolecular screening
US6770486B1 (en) * 2000-02-08 2004-08-03 Isis Pharmaceuticals, Inc. Optimization of ligand affinity for RNA targets using mass spectrometry

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6051378A (en) * 1996-03-04 2000-04-18 Genetrace Systems Inc. Methods of screening nucleic acids using mass spectrometry
US6453244B1 (en) * 2000-02-10 2002-09-17 Stanford University Detection of polymorphisms by denaturing high-performance liquid chromatography
US6486309B1 (en) * 2001-08-01 2002-11-26 Varian, Inc. Method of detecting sequence variations in macromolecules by thermal gradiant

Also Published As

Publication number Publication date
WO2005019801A2 (en) 2005-03-03
US20050095622A1 (en) 2005-05-05
US20070264661A1 (en) 2007-11-15

Similar Documents

Publication Publication Date Title
WO2006044728A3 (en) Methods for isolation of nucleic acids from prokaryotic spores
WO2006078859A3 (en) Methods for separating compounds
WO2001051919A3 (en) High-throughput formation, identification, and analysis of diverse solid-forms
WO2006081331A3 (en) Quinoxaline derivatives as antitumor agents
WO2002084250A3 (en) Methods for mass spectrometry detection and quantification of specific target proteins in complex biological samples
AU2003224674A1 (en) Compounds and methods for analyzing the proteome
WO2005111212A3 (en) Biological bar code
WO2007065025A3 (en) Method of dna analysis using micro/nanochannel
WO2005012574A3 (en) Biological bar-code
WO2005023986A3 (en) Microrna as ligands and target molecules
TW200704927A (en) Mass spectrometric analysis of ligand conjugated magnetic nanoparticles
AU6380001A (en) Porous ferro- or ferrimagnetic glass particles for isolating molecules
WO2008067547A3 (en) Improved immunoglobulin libraries
MX2007000103A (en) Universal antibody libraries.
WO2007022241A3 (en) Novel high affinity quinoline-based kinase ligands
WO2009061904A3 (en) MASS SPECTROMETRY ASSAY FOR eIF4E AND eIF4E REGULON ACTIVITY
WO2004076632A3 (en) Methods of screening for trpm5 modulators
WO2005040107A3 (en) Methods for making simvastatin and intermediates
WO2006116893A3 (en) Mass spectrometric analysis method
WO2006081553A3 (en) ANTI-MUC1 α/ß ANTIBODIES
TW200637830A (en) Erastin and erastin binding proteins, and uses thereof
WO2005019801A3 (en) Method for rapid identification of molecules with functional activity towards biomolecular targets
AU2003234730A1 (en) Centrifugal cytology system, chamber block and method for the preparation of treated monolayers of sample material
WO2002068931A3 (en) Screening for enzyme stereoselectivity utilizing mass spectrometry
WO2007095378A3 (en) Mass tagging for quantitative analysis of biomolecules using 13c labeled phenylisocyanate

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase