WO2005028615A3 - Methods and compositions for detecting promoter activity and expressing fusion proteins - Google Patents
Methods and compositions for detecting promoter activity and expressing fusion proteins Download PDFInfo
- Publication number
- WO2005028615A3 WO2005028615A3 PCT/US2004/020747 US2004020747W WO2005028615A3 WO 2005028615 A3 WO2005028615 A3 WO 2005028615A3 US 2004020747 W US2004020747 W US 2004020747W WO 2005028615 A3 WO2005028615 A3 WO 2005028615A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- fusion proteins
- compositions
- promoter activity
- nucleic acid
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP04809464A EP1644538A4 (en) | 2003-06-26 | 2004-06-28 | Methods and compositions for detecting promoter activity and expressing fusion proteins |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US48250403P | 2003-06-26 | 2003-06-26 | |
US60/482,504 | 2003-06-26 | ||
US48730103P | 2003-07-16 | 2003-07-16 | |
US60/487,301 | 2003-07-16 | ||
US51163403P | 2003-10-17 | 2003-10-17 | |
US60/511,634 | 2003-10-17 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2005028615A2 WO2005028615A2 (en) | 2005-03-31 |
WO2005028615A3 true WO2005028615A3 (en) | 2005-08-25 |
Family
ID=34381933
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2004/020747 WO2005028615A2 (en) | 2003-06-26 | 2004-06-28 | Methods and compositions for detecting promoter activity and expressing fusion proteins |
Country Status (3)
Country | Link |
---|---|
US (4) | US20050095615A1 (en) |
EP (1) | EP1644538A4 (en) |
WO (1) | WO2005028615A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8945884B2 (en) | 2000-12-11 | 2015-02-03 | Life Technologies Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites |
US9534252B2 (en) | 2003-12-01 | 2017-01-03 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
Families Citing this family (11)
Publication number | Priority date | Publication date | Assignee | Title |
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US7670823B1 (en) * | 1999-03-02 | 2010-03-02 | Life Technologies Corp. | Compositions for use in recombinational cloning of nucleic acids |
US6720140B1 (en) * | 1995-06-07 | 2004-04-13 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
US6143557A (en) * | 1995-06-07 | 2000-11-07 | Life Technologies, Inc. | Recombination cloning using engineered recombination sites |
CA2307016A1 (en) * | 1997-10-24 | 1999-05-06 | Life Technologies, Inc. | Recombinational cloning using nucleic acids having recombination sites |
CN101125873A (en) * | 1997-10-24 | 2008-02-20 | 茵维特罗根公司 | Recombinational cloning using nucleic acids having recombination sites |
EP1978098A3 (en) * | 1999-12-10 | 2009-02-18 | Invitrogen Corporation | Use of multiple recombination sites with unique specificity in recombinational cloning |
WO2005054427A2 (en) * | 2003-10-22 | 2005-06-16 | Invitrogen Corporation | Target sequences for synthetic molecules |
WO2005040197A1 (en) * | 2003-10-24 | 2005-05-06 | Invitrogen Corporation | Compositions, methods and kits for biarsenical fluorophore labeling |
EP1760092A1 (en) * | 2005-08-26 | 2007-03-07 | Applied Research Systems ARS Holding N.V. | System for screening cells for high expression of a protein of interest |
WO2007047706A2 (en) | 2005-10-17 | 2007-04-26 | Children's Hospital | Methods and compositions for regulating gene expression |
AU2009298192B2 (en) * | 2008-10-03 | 2015-01-22 | Xoma Technology Ltd. | Novel triple tag sequences and methods of use thereof |
Citations (1)
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US6468754B1 (en) * | 2000-04-18 | 2002-10-22 | Iconix Pharmaceuticals, Inc. | Vector and method for targeted replacement and disruption of an integrated DNA sequence |
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-
2004
- 2004-06-28 US US10/877,952 patent/US20050095615A1/en not_active Abandoned
- 2004-06-28 WO PCT/US2004/020747 patent/WO2005028615A2/en active Application Filing
- 2004-06-28 EP EP04809464A patent/EP1644538A4/en not_active Withdrawn
-
2008
- 2008-01-25 US US12/019,785 patent/US20090176975A1/en not_active Abandoned
-
2011
- 2011-03-21 US US13/053,040 patent/US20110269120A1/en not_active Abandoned
-
2012
- 2012-07-02 US US13/540,574 patent/US20140051843A1/en not_active Abandoned
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9309520B2 (en) | 2000-08-21 | 2016-04-12 | Life Technologies Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
US8945884B2 (en) | 2000-12-11 | 2015-02-03 | Life Technologies Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiplerecognition sites |
US9534252B2 (en) | 2003-12-01 | 2017-01-03 | Life Technologies Corporation | Nucleic acid molecules containing recombination sites and methods of using the same |
Also Published As
Publication number | Publication date |
---|---|
US20140051843A1 (en) | 2014-02-20 |
US20050095615A1 (en) | 2005-05-05 |
US20110269120A1 (en) | 2011-11-03 |
US20090176975A1 (en) | 2009-07-09 |
EP1644538A4 (en) | 2006-11-08 |
EP1644538A2 (en) | 2006-04-12 |
WO2005028615A2 (en) | 2005-03-31 |
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