WO2005044979A3 - Glycosylation enzymes and systems and methods of making and using them - Google Patents

Glycosylation enzymes and systems and methods of making and using them Download PDF

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Publication number
WO2005044979A3
WO2005044979A3 PCT/US2004/025015 US2004025015W WO2005044979A3 WO 2005044979 A3 WO2005044979 A3 WO 2005044979A3 US 2004025015 W US2004025015 W US 2004025015W WO 2005044979 A3 WO2005044979 A3 WO 2005044979A3
Authority
WO
WIPO (PCT)
Prior art keywords
glycosylated
derivatives
novel
systems
glycosylated derivatives
Prior art date
Application number
PCT/US2004/025015
Other languages
French (fr)
Other versions
WO2005044979A2 (en
Inventor
Axel Trefzer
Brian D Green
Mervyn Bibb
Dylan Mason
Original Assignee
Diversa Corp
Axel Trefzer
Brian D Green
Mervyn Bibb
Dylan Mason
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Diversa Corp, Axel Trefzer, Brian D Green, Mervyn Bibb, Dylan Mason filed Critical Diversa Corp
Publication of WO2005044979A2 publication Critical patent/WO2005044979A2/en
Publication of WO2005044979A3 publication Critical patent/WO2005044979A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system

Abstract

The invention provides a novel technology for glycosylation of natural products using novel genetically engineered strains of bacteria. These in vivo glycosylation systems of the invention express a heterologous glycosyltransferase and deoxysugar pathway that are capable of glycosylating a suitable substrate, which can be added to a culture broth. The invention also provides novel compounds glycosylated by these novel genetically engineered strains (the in vivo glycosylation systems of the invention), including novel peptides, mixed polyketide-peptides, or polyketides, including novel macrolides, e.g., glycosylated spinosyn derivatives, glycosylated derivatives of antibiotics such as erythromycin, tetracycline, rifampicin, glycosylated derivatives of anti-tumor drugs such as daunorubicin, mithramycin, derivatives of immunosuppressants such as rapamycin, FK520, FK506, glycosylated derivatives of anti-fungals such as amphotericin, glycosylated derivatives of antibacterials such as tylosin, glycosylated derivatives of antiparasitics such as avermectin, glycosylated derivatives of insecticides such as spinosyn, and methods for making these compounds using the in vivo glycosylation systems of the invention.
PCT/US2004/025015 2003-08-04 2004-08-04 Glycosylation enzymes and systems and methods of making and using them WO2005044979A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US49278103P 2003-08-04 2003-08-04
US60/492,781 2003-08-04
US51595003P 2003-10-29 2003-10-29
US60/515,950 2003-10-29

Publications (2)

Publication Number Publication Date
WO2005044979A2 WO2005044979A2 (en) 2005-05-19
WO2005044979A3 true WO2005044979A3 (en) 2009-04-09

Family

ID=34576538

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/025015 WO2005044979A2 (en) 2003-08-04 2004-08-04 Glycosylation enzymes and systems and methods of making and using them

Country Status (1)

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WO (1) WO2005044979A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110171691A1 (en) * 2008-09-11 2011-07-14 W.C. Heraeus Gmbh Genetically modified strains for biotransformations in anthracycline production
CN103755723B (en) * 2014-02-07 2016-04-20 天津大学 A kind of preparation method of rifampicin I crystal form
CN112386603B (en) * 2015-06-01 2021-10-01 中南大学 Application of spinosyn derivative in preparation of anti-tumor drugs and anti-KSHV virus drugs
CN106006882A (en) * 2016-07-18 2016-10-12 青岛阳光动力生物医药技术有限公司 Photosensitive molecule system disinfectant combination used for aquaculture
CN114438004B (en) * 2021-12-14 2023-08-22 湖南师范大学 Saccharopolyspora erythraea engineering strain with doubled pII gene, and construction method and application thereof
CN114686549A (en) * 2022-04-29 2022-07-01 陕西嘉禾生物科技股份有限公司 Method for preparing enzyme modified isoquercitrin by using rutin

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265202B1 (en) * 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6265202B1 (en) * 1998-06-26 2001-07-24 Regents Of The University Of Minnesota DNA encoding methymycin and pikromycin

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MIYMOTO, Y.: "Cloning and characterization of a glycosyltransferase gene involved in the biosynthesis ofanthacycline antibiotic beta-rhudomycin from Streptomyces Vilaceus", FEMS MICROBIOL LETT., 10 December 2001 (2001-12-10), pages 163 - 168 *
RODRIGUEZ, L. ET AL.: "Engineering Deoxysugar Biosynthetic Pathways from Antibiotic- Producing Microorganisms: A tool to produce Novel Glycosylated Bioactive Compounds", CHEM & BIOL, vol. 9, June 2002 (2002-06-01), pages 721 - 729 *

Also Published As

Publication number Publication date
WO2005044979A2 (en) 2005-05-19

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