WO2005072507A3 - Biomarker detection in circulating cells - Google Patents

Biomarker detection in circulating cells Download PDF

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Publication number
WO2005072507A3
WO2005072507A3 PCT/US2005/000998 US2005000998W WO2005072507A3 WO 2005072507 A3 WO2005072507 A3 WO 2005072507A3 US 2005000998 W US2005000998 W US 2005000998W WO 2005072507 A3 WO2005072507 A3 WO 2005072507A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomarkers
cells
biomarker detection
circulating cells
detection
Prior art date
Application number
PCT/US2005/000998
Other languages
French (fr)
Other versions
WO2005072507A2 (en
Inventor
Hrair Kirakossian
Thomas Klopack
Sharat Singh
Original Assignee
Monogram Biosciences Inc
Hrair Kirakossian
Thomas Klopack
Sharat Singh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monogram Biosciences Inc, Hrair Kirakossian, Thomas Klopack, Sharat Singh filed Critical Monogram Biosciences Inc
Publication of WO2005072507A2 publication Critical patent/WO2005072507A2/en
Publication of WO2005072507A3 publication Critical patent/WO2005072507A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5091—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing the pathological state of an organism
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures

Abstract

A method is provided for detecting biomarkers in rare circulating cells by forming an enriched population of cells immunomagnetically followed by biomarker detection using binding compounds having releasable molecular tags. Preferably, biomarkers comprising one or more protein-protein complexes are detected in circulating cancer cells metastasized from a solid tumor. In the presence of biomarkers, the molecular tags of the binding compounds are released and separated from the assay mixture for detection and/or quantification.
PCT/US2005/000998 2004-01-26 2005-01-13 Biomarker detection in circulating cells WO2005072507A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/765,773 US7537938B2 (en) 2000-04-28 2004-01-26 Biomarker detection in circulating cells
US10/765,773 2004-01-26

Publications (2)

Publication Number Publication Date
WO2005072507A2 WO2005072507A2 (en) 2005-08-11
WO2005072507A3 true WO2005072507A3 (en) 2009-04-09

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/000998 WO2005072507A2 (en) 2004-01-26 2005-01-13 Biomarker detection in circulating cells

Country Status (2)

Country Link
US (1) US7537938B2 (en)
WO (1) WO2005072507A2 (en)

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US7537938B2 (en) 2009-05-26
US20040157271A1 (en) 2004-08-12
WO2005072507A2 (en) 2005-08-11

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