WO2005075656A8 - Biochip electroporator and its use in multi-site, single-cell electroporation - Google Patents

Biochip electroporator and its use in multi-site, single-cell electroporation

Info

Publication number
WO2005075656A8
WO2005075656A8 PCT/EP2004/000789 EP2004000789W WO2005075656A8 WO 2005075656 A8 WO2005075656 A8 WO 2005075656A8 EP 2004000789 W EP2004000789 W EP 2004000789W WO 2005075656 A8 WO2005075656 A8 WO 2005075656A8
Authority
WO
WIPO (PCT)
Prior art keywords
electroporation
cell
adhering
electroporator
biochip
Prior art date
Application number
PCT/EP2004/000789
Other languages
French (fr)
Other versions
WO2005075656A1 (en
Inventor
Stefano Vassanelli
Giorgio Cellere
Mauro Borgo
Leonardo Bandiera
Original Assignee
Biosilab S R L
Stefano Vassanelli
Giorgio Cellere
Mauro Borgo
Leonardo Bandiera
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to JP2006549881A priority Critical patent/JP4588719B2/en
Application filed by Biosilab S R L, Stefano Vassanelli, Giorgio Cellere, Mauro Borgo, Leonardo Bandiera filed Critical Biosilab S R L
Priority to PCT/EP2004/000789 priority patent/WO2005075656A1/en
Priority to DE602004025146T priority patent/DE602004025146D1/en
Priority to US10/587,681 priority patent/US8017367B2/en
Priority to AT04706137T priority patent/ATE455180T1/en
Priority to CNB2004800410833A priority patent/CN100497641C/en
Priority to CA2554027A priority patent/CA2554027C/en
Priority to AU2004315392A priority patent/AU2004315392B2/en
Priority to EP04706137A priority patent/EP1720991B1/en
Priority to DK04706137.9T priority patent/DK1720991T3/en
Publication of WO2005075656A1 publication Critical patent/WO2005075656A1/en
Publication of WO2005075656A8 publication Critical patent/WO2005075656A8/en
Priority to IL177110A priority patent/IL177110A0/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02Electrical or electromagnetic means, e.g. for electroporation or for cell fusion

Abstract

The introduction of genetic material or molecules of biological interest into cells is a procedure with an increasing interest both for experimental and application purposes, so that electroporation is a widely used technique, but the electroporation of single adhering cells is still impaired. The present application describes an apparatus for the electroporation of any kind of cell adhering to a substrate at any stage of development, where an electrical signal can be driven and applied to a single adhering cell in culture in order to obtain its electroporation. The method to electroporate a single adhering cell with the apparatus of the invention is also described.
PCT/EP2004/000789 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation WO2005075656A1 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CNB2004800410833A CN100497641C (en) 2004-01-29 2004-01-29 Biochip electroporator and its application in omnibearing single-cell electroporation
PCT/EP2004/000789 WO2005075656A1 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation
DE602004025146T DE602004025146D1 (en) 2004-01-29 2004-01-29 BIOCHIP ELECTROPORATION DEVICE WITH SEVERAL POSITIONS FOR INDIVIDUAL ELECTROPORATION
US10/587,681 US8017367B2 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation
AT04706137T ATE455180T1 (en) 2004-01-29 2004-01-29 BIOCHIP ELECTROPORATION DEVICE WITH MULTIPLE POSITIONS FOR SINGLE CELL ELECTROPORATION
JP2006549881A JP4588719B2 (en) 2004-01-29 2004-01-29 Biochip-type electroporator and use in single-cell electroporation at multiple sites
CA2554027A CA2554027C (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation
DK04706137.9T DK1720991T3 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-position single-cell electroporation
EP04706137A EP1720991B1 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation
AU2004315392A AU2004315392B2 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation
IL177110A IL177110A0 (en) 2004-01-29 2006-07-26 Biochip electroporator and its use in multi-site, single-cell electroporation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/EP2004/000789 WO2005075656A1 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation

Publications (2)

Publication Number Publication Date
WO2005075656A1 WO2005075656A1 (en) 2005-08-18
WO2005075656A8 true WO2005075656A8 (en) 2005-11-17

Family

ID=34833863

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2004/000789 WO2005075656A1 (en) 2004-01-29 2004-01-29 Biochip electroporator and its use in multi-site, single-cell electroporation

Country Status (11)

Country Link
US (1) US8017367B2 (en)
EP (1) EP1720991B1 (en)
JP (1) JP4588719B2 (en)
CN (1) CN100497641C (en)
AT (1) ATE455180T1 (en)
AU (1) AU2004315392B2 (en)
CA (1) CA2554027C (en)
DE (1) DE602004025146D1 (en)
DK (1) DK1720991T3 (en)
IL (1) IL177110A0 (en)
WO (1) WO2005075656A1 (en)

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US7266457B1 (en) * 1999-05-21 2007-09-04 Hesperos, Llc High throughput functional genomics
KR20070077320A (en) * 2006-01-23 2007-07-26 삼성전자주식회사 Cell culture chip and method for monitoring cell culture in real time
ITTO20060273A1 (en) 2006-04-12 2007-10-13 Silicon Biosystem S P A METHODS AND EQUIPMENT FOR THE SELECTION AND / OR PROCESSING OF PARTICLES, IN PARTICULAR FOR THE SELECTIVE AND / OR OPTIMIZED CELL LYSIS
ITTO20060278A1 (en) * 2006-04-13 2007-10-14 Silicon Biosystem S P A METHOD FOR THE SELECTION AND / OR PROCESSING OF PARTICLES, IN PARTICULAR CELLS
EP2118261A4 (en) 2007-03-01 2013-04-10 Univ Kingston Planar electroporation apparatus and method
CN101038284B (en) 2007-04-25 2011-04-27 博奥生物有限公司 Method for enhancing electric impedance detecting sensibility of electric impedance detecting device
WO2009029613A1 (en) * 2007-08-31 2009-03-05 The Board Of Regents, The University Of Texas System Apparatus for performing magnetic electroporation
US20090087900A1 (en) * 2007-09-28 2009-04-02 Kent Davey Apparatus for Performing Electrodistention on Algae Cells
CN101556273B (en) * 2008-04-08 2013-03-20 博奥生物有限公司 Method for analyzing cell migration by resistance sensing resistant technology and special device thereof
US8008063B2 (en) * 2008-06-24 2011-08-30 Bio-Rad Laboratories, Inc. Individual-cell electroporation using area-focused electric fields
CN101614729B (en) * 2008-06-27 2013-04-24 博奥生物有限公司 Microelectrode array device and special device for cell manipulation and electrophysiological signal detection
EP2147697A1 (en) * 2008-07-21 2010-01-27 Centre National De La Recherche Scientifique-CNRS Process and device for applying electric fields into conductive material
CN101486964B (en) * 2009-03-06 2012-01-11 北京大学 Cell electroporation method based on micro-fluidic chip and special chip therefor
US9304132B2 (en) 2009-04-16 2016-04-05 President And Fellows Of Harvard College Molecular delivery with nanowires
US20110065161A1 (en) * 2009-09-14 2011-03-17 Board Of Regents, The University Of Texas System Bipolar solid state marx generator
CN101870949B (en) * 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 Electroporated chip and porous plate device base on electroporated chip
EP2399984B1 (en) * 2010-06-22 2013-03-20 Lonza Cologne GmbH Method and electrode assembly for treating adherent cells
KR20130096744A (en) * 2010-09-29 2013-08-30 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Molecular delivery with nanowires
EP3260163B1 (en) * 2014-05-02 2019-07-17 Lonza Cologne GmbH Device and method for large volume transfection
WO2016112315A2 (en) 2015-01-09 2016-07-14 President And Fellows Of Harvard College Nanowire arrays for neurotechnology and other applications
LU92920B1 (en) * 2015-12-21 2017-07-13 Luxembourg Inst Science & Tech List Electrochemical reactor to control the pH in miniaturized dimensions
CN114933967A (en) * 2020-10-13 2022-08-23 北京航空航天大学 Nano-electroporation device based on single cell array and application thereof
CN113528332B (en) * 2021-07-19 2023-10-24 中山大学 Intracellular and extracellular electrophysiological recording system and method for automatic electroporation regulation screening
CN115120859A (en) * 2022-05-18 2022-09-30 华南师范大学 High-flux nano electroporation/nano gene fusion chip and preparation method and application thereof
CN116121063B (en) * 2022-12-30 2023-08-04 山东大学 Biochip for realizing magnetic field regulation and temperature monitoring and preparation method thereof

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US5134070A (en) * 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
US6352535B1 (en) * 1997-09-25 2002-03-05 Nanoptics, Inc. Method and a device for electro microsurgery in a physiological liquid environment
US6132683A (en) * 1998-12-23 2000-10-17 Matsushita Electric Industrial Co., Ltd. Cell potential measuring electrode and measuring apparatus using the same
DE19841337C1 (en) * 1998-05-27 1999-09-23 Micronas Intermetall Gmbh Intracellular manipulation of biological cell contents, assisting injection or removal of substances or cell components
CN1185492C (en) * 1999-03-15 2005-01-19 清华大学 Single-point strobed micro electromagnetic units array chip or electromagnetic biologic chip and application thereof
US6300108B1 (en) * 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US20020090649A1 (en) * 1999-12-15 2002-07-11 Tony Chan High density column and row addressable electrode arrays
US7306924B2 (en) * 2000-04-17 2007-12-11 Purdue Research Foundation Biosensor and related method
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US7521224B2 (en) * 2003-09-30 2009-04-21 The United States Of America As Represented By The Secretary Of The Navy Microelectronic cell electroporation array

Also Published As

Publication number Publication date
US8017367B2 (en) 2011-09-13
AU2004315392A1 (en) 2005-08-18
DK1720991T3 (en) 2010-05-25
CA2554027A1 (en) 2005-08-18
JP4588719B2 (en) 2010-12-01
ATE455180T1 (en) 2010-01-15
WO2005075656A1 (en) 2005-08-18
EP1720991B1 (en) 2010-01-13
DE602004025146D1 (en) 2010-03-04
IL177110A0 (en) 2006-12-10
CA2554027C (en) 2013-10-08
AU2004315392B2 (en) 2010-04-22
CN100497641C (en) 2009-06-10
US20070155015A1 (en) 2007-07-05
EP1720991A1 (en) 2006-11-15
CN1906305A (en) 2007-01-31
JP2007519405A (en) 2007-07-19

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