WO2005085849A3 - Biohazard identification by fluorescent immunoassay and single molecule detection - Google Patents

Biohazard identification by fluorescent immunoassay and single molecule detection Download PDF

Info

Publication number
WO2005085849A3
WO2005085849A3 PCT/US2004/035454 US2004035454W WO2005085849A3 WO 2005085849 A3 WO2005085849 A3 WO 2005085849A3 US 2004035454 W US2004035454 W US 2004035454W WO 2005085849 A3 WO2005085849 A3 WO 2005085849A3
Authority
WO
WIPO (PCT)
Prior art keywords
identification
biohazard
single molecule
molecule detection
fluorescent immunoassay
Prior art date
Application number
PCT/US2004/035454
Other languages
French (fr)
Other versions
WO2005085849A2 (en
Inventor
Jonathan W Larson
Original Assignee
Us Genomics Inc
Jonathan W Larson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Genomics Inc, Jonathan W Larson filed Critical Us Genomics Inc
Publication of WO2005085849A2 publication Critical patent/WO2005085849A2/en
Publication of WO2005085849A3 publication Critical patent/WO2005085849A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y10/00—Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/30—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Mycoplasmatales, e.g. Pleuropneumonia-like organisms [PPLO]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/35—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Mycobacteriaceae (F)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

Abstract

The invention provides methods and systems for real time detection and identification of biohazardous agents such as weaponized bacteria and the like.
PCT/US2004/035454 2003-10-24 2004-10-25 Biohazard identification by fluorescent immunoassay and single molecule detection WO2005085849A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51445503P 2003-10-24 2003-10-24
US60/514,455 2003-10-24

Publications (2)

Publication Number Publication Date
WO2005085849A2 WO2005085849A2 (en) 2005-09-15
WO2005085849A3 true WO2005085849A3 (en) 2006-05-11

Family

ID=34919305

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2004/035454 WO2005085849A2 (en) 2003-10-24 2004-10-25 Biohazard identification by fluorescent immunoassay and single molecule detection

Country Status (1)

Country Link
WO (1) WO2005085849A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109239040A (en) * 2018-10-09 2019-01-18 太原理工大学 It is a kind of based on aptamer chain-black phosphorus nanometer sheet fluorescence resonance energy transfer arsenic ion detection method

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US8999636B2 (en) 2007-01-08 2015-04-07 Toxic Report Llc Reaction chamber
US8361716B2 (en) 2008-10-03 2013-01-29 Pathogenetix, Inc. Focusing chamber
EP2437061A1 (en) * 2010-09-30 2012-04-04 Ikonomopoulos, John Mycobacterial protein detection
US8685708B2 (en) 2012-04-18 2014-04-01 Pathogenetix, Inc. Device for preparing a sample
US8956815B2 (en) 2012-04-18 2015-02-17 Toxic Report Llc Intercalation methods and devices
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample
WO2017142974A1 (en) * 2016-02-16 2017-08-24 Isi Life Sciences, Inc. Assays and methods for detection of pathogenic bacteria in a foodstuff using fluorescein-bound antibodies
CN109085716A (en) * 2018-09-21 2018-12-25 福州大学 It is a kind of based on micro-fluidic and technology of quantum dots colorized optical filtering membrane preparation method
EP3953489A1 (en) * 2019-04-12 2022-02-16 Miltenyi Biotec B.V. & Co. KG Conjugates having an enzymatically releasable detection moiety and a barcode moiety
CN111077298A (en) * 2019-12-21 2020-04-28 东南大学 Preparation method and application of immunofluorescence co-localization imaging platform
EP4211471A1 (en) * 2020-09-07 2023-07-19 Miltenyi Biotec B.V. & Co. KG Conjugates having an enzymatically releasable detection moiety and a barcode moiety
WO2022119896A1 (en) * 2020-12-01 2022-06-09 Pathogendx, Inc. Methods for detecting airborne viruses in air samples
CN113484515A (en) * 2021-06-11 2021-10-08 江苏大学 Method and system for rapidly detecting food-borne pathogenic bacteria

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006877A1 (en) * 1993-09-03 1995-03-09 Behringwerke Ag Fluorescent oxygen channeling immunoassays
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
WO2000009757A1 (en) * 1998-08-13 2000-02-24 U.S. Genomics, Inc. Optically characterizing polymers
EP0990903A1 (en) * 1998-09-18 2000-04-05 Massachusetts Institute Of Technology Biological applications of semiconductor nanocrystals

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995006877A1 (en) * 1993-09-03 1995-03-09 Behringwerke Ag Fluorescent oxygen channeling immunoassays
WO1998035012A2 (en) * 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
WO2000009757A1 (en) * 1998-08-13 2000-02-24 U.S. Genomics, Inc. Optically characterizing polymers
EP0990903A1 (en) * 1998-09-18 2000-04-05 Massachusetts Institute Of Technology Biological applications of semiconductor nanocrystals

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GOLDMAN ELLEN R ET AL: "Avidin: a natural bridge for quantum dot-antibody conjugates", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, WASHINGTON, DC, US, vol. 124, no. 22, 5 June 2002 (2002-06-05), pages 6378 - 6382, XP002308426, ISSN: 0002-7863 *
ROWE-TAITT C A ET AL: "ARRAY BIOSENSOR FOR DETECTION OF BIOHAZARDS", BIOSENSORS & BIOELECTRONICS, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 14, no. 10/11, January 2000 (2000-01-01), pages 785 - 794, XP008046590, ISSN: 0956-5663 *
ROWE-TAITT CHRIS A ET AL: "Simultaneous detection of six biohazardous agents using a planar waveguide array biosensor", BIOSENSORS AND BIOELECTRONICS, vol. 15, no. 11-12, December 2000 (2000-12-01), pages 579 - 589, XP002344240, ISSN: 0956-5663 *
SAPSFORD K E ET AL: "Fluorescence-based array biosensors for detection of biohazards.", JOURNAL OF APPLIED MICROBIOLOGY, vol. 96, no. 1, 2004, pages 47 - 58, XP002344239, ISSN: 1364-5072 *
XU HONGXIA ET AL: "Multiplexed SNP genotyping using the Qbead system: a quantum dot-encoded microsphere-based assay", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 31, no. 8, 15 April 2003 (2003-04-15), pages e43, XP002308425, ISSN: 0305-1048 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109239040A (en) * 2018-10-09 2019-01-18 太原理工大学 It is a kind of based on aptamer chain-black phosphorus nanometer sheet fluorescence resonance energy transfer arsenic ion detection method

Also Published As

Publication number Publication date
WO2005085849A2 (en) 2005-09-15

Similar Documents

Publication Publication Date Title
WO2005085849A3 (en) Biohazard identification by fluorescent immunoassay and single molecule detection
WO2005111212A3 (en) Biological bar code
WO2006029001A3 (en) System for dispensing biological fluids
EP2305809A3 (en) Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
AU2001250882A1 (en) Inorganic particle conjugates
WO2005012574A3 (en) Biological bar-code
DE60335722D1 (en) METHOD AND DEVICES FOR ANALYSIS OF SEALED CONTAINERS
AU2002350946A1 (en) Device for chemical or biochemical analysis
WO2004053062A3 (en) Bentgrass event asr-368 and compositions and methods for detection thereof
GB2423151B (en) Examination systems for biological samples
EP1700120A4 (en) Marker for neuromyelitis optica
IL164224A0 (en) Point source biological agent detection system
EP2298921A3 (en) Cotton event MON 88913 and compositions and methods for detection thereof
WO2008008797A3 (en) Luminescent 1-hydroxy-2-pyridinone chelates of lanthanides
WO2005045075A3 (en) Sample preparation methods and devices
WO2007136893A3 (en) Anti-fgf19 antibodies and methods using same
IL173843A0 (en) Assay for coronaviruses, reagents for use therein and kits containing the same
WO2007149065A3 (en) Methods and apparatuses for detecting chemical or biological agents
AU2002364120A1 (en) Sensor for the use in testing biological chemical or environmental samples
EP2295979A3 (en) Protein detection reagents and methods with dyes and dextrins
WO2006099537A3 (en) The use of antibody-surrogate antigen systems for detection of analytes
WO2007078268A3 (en) Methods and compositions for the detection and/or quantification of gram positive bacterial contaminants
WO2007035763A3 (en) Methods for detecting and characterizing microorganisms
WO2003027236A3 (en) Systems, methods and kits for remote genetic analysis and consultation
EP2182065A3 (en) Staphylococcus epidermidis antigens

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BW BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE EG ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NA NI NO NZ OM PG PH PL PT RO RU SC SD SE SG SK SL SY TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): BW GH GM KE LS MW MZ NA SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PL PT RO SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
122 Ep: pct application non-entry in european phase