WO2005095590A3 - Sperm suspensions for sorting into x or y chromosome-bearing enriched populations - Google Patents

Sperm suspensions for sorting into x or y chromosome-bearing enriched populations Download PDF

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Publication number
WO2005095590A3
WO2005095590A3 PCT/US2005/010481 US2005010481W WO2005095590A3 WO 2005095590 A3 WO2005095590 A3 WO 2005095590A3 US 2005010481 W US2005010481 W US 2005010481W WO 2005095590 A3 WO2005095590 A3 WO 2005095590A3
Authority
WO
WIPO (PCT)
Prior art keywords
sorting
sperm
chromosome
enriched populations
suspensions
Prior art date
Application number
PCT/US2005/010481
Other languages
French (fr)
Other versions
WO2005095590A2 (en
Inventor
Cindy L Ludwig
Kathleen S Crowley
Charles N Graves
Original Assignee
Monsanto Technology Llc
Cindy L Ludwig
Kathleen S Crowley
Charles N Graves
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc, Cindy L Ludwig, Kathleen S Crowley, Charles N Graves filed Critical Monsanto Technology Llc
Priority to BRPI0509488-7A priority Critical patent/BRPI0509488A/en
Priority to NZ550196A priority patent/NZ550196A/en
Priority to JP2007506461A priority patent/JP2007531523A/en
Priority to AU2005228893A priority patent/AU2005228893B2/en
Priority to CN2005800173705A priority patent/CN101014700B/en
Priority to MXPA06011344A priority patent/MXPA06011344A/en
Priority to CA2561661A priority patent/CA2561661C/en
Priority to EP05731409A priority patent/EP1730266A2/en
Publication of WO2005095590A2 publication Critical patent/WO2005095590A2/en
Publication of WO2005095590A3 publication Critical patent/WO2005095590A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/48—Reproductive organs
    • A61K35/52—Sperm; Prostate; Seminal fluid; Leydig cells of testes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/02—Preservation of living parts
    • A01N1/0205—Chemical aspects
    • A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
    • A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/061—Sperm cells, spermatogonia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis

Abstract

Sperm cell suspensions comprising a motility inhibitor are disclosed. The cells contained in such suspensions tend to have a greater capacity for enduring the various process steps typically associated with the sorting of sperm cells into gender enriched populations, thereby resulting in post-sort compositions with an increased number of viable or motile sperm. Processes for forming such cell suspensions, as well as processes for staining sperm cells, are also disclosed.
PCT/US2005/010481 2004-03-29 2005-03-29 Sperm suspensions for sorting into x or y chromosome-bearing enriched populations WO2005095590A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
BRPI0509488-7A BRPI0509488A (en) 2004-03-29 2005-03-29 sperm suspensions for selection of enriched populations carrying x or y chromosome
NZ550196A NZ550196A (en) 2004-03-29 2005-03-29 Method for sorting sperm cells into X or Y chromosome-bearing enriched populations comprising a composition which inhibits motility and a DNA-selective dye
JP2007506461A JP2007531523A (en) 2004-03-29 2005-03-29 Sperm suspension for sorting into X or Y chromosome-bearing enriched populations
AU2005228893A AU2005228893B2 (en) 2004-03-29 2005-03-29 Sperm suspensions for sorting into X or Y chromosome-bearing enriched populations
CN2005800173705A CN101014700B (en) 2004-03-29 2005-03-29 Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
MXPA06011344A MXPA06011344A (en) 2004-03-29 2005-03-29 Sperm suspensions for sorting into x or y chromosome-bearing enriched populations.
CA2561661A CA2561661C (en) 2004-03-29 2005-03-29 Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
EP05731409A EP1730266A2 (en) 2004-03-29 2005-03-29 Sperm suspensions for sorting into x or y chromosome-bearing enriched populations

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US55740704P 2004-03-29 2004-03-29
US60/557,407 2004-03-29
US61417804P 2004-09-29 2004-09-29
US60/614,178 2004-09-29
US61844004P 2004-10-13 2004-10-13
US60/618,440 2004-10-13

Publications (2)

Publication Number Publication Date
WO2005095590A2 WO2005095590A2 (en) 2005-10-13
WO2005095590A3 true WO2005095590A3 (en) 2005-12-08

Family

ID=34964337

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2005/010481 WO2005095590A2 (en) 2004-03-29 2005-03-29 Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
PCT/US2005/010599 WO2005094852A2 (en) 2004-03-29 2005-03-29 Sperm suspensions for use in insemination

Family Applications After (1)

Application Number Title Priority Date Filing Date
PCT/US2005/010599 WO2005094852A2 (en) 2004-03-29 2005-03-29 Sperm suspensions for use in insemination

Country Status (16)

Country Link
US (2) US7838210B2 (en)
EP (5) EP1730266A2 (en)
JP (4) JP2007537727A (en)
CN (2) CN102586179B (en)
AR (2) AR048515A1 (en)
AU (2) AU2005228893B2 (en)
BR (2) BRPI0509488A (en)
CA (3) CA2972254A1 (en)
DK (2) DK2801363T3 (en)
ES (1) ES2397678T3 (en)
HK (2) HK1173745A1 (en)
MX (2) MXPA06011345A (en)
NZ (2) NZ550196A (en)
PL (1) PL2151243T3 (en)
PT (1) PT2151243E (en)
WO (2) WO2005095590A2 (en)

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