WO2006005074A3 - Novel hot start nucleic acid amplification - Google Patents

Novel hot start nucleic acid amplification Download PDF

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Publication number
WO2006005074A3
WO2006005074A3 PCT/US2005/023824 US2005023824W WO2006005074A3 WO 2006005074 A3 WO2006005074 A3 WO 2006005074A3 US 2005023824 W US2005023824 W US 2005023824W WO 2006005074 A3 WO2006005074 A3 WO 2006005074A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid amplification
reaction mixture
hot start
novel hot
Prior art date
Application number
PCT/US2005/023824
Other languages
French (fr)
Other versions
WO2006005074A2 (en
Inventor
Christopher J Kubu
Jeannine C Muller-Greven
Robert B Moffett
Original Assignee
Usb Corp
Christopher J Kubu
Jeannine C Muller-Greven
Robert B Moffett
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Usb Corp, Christopher J Kubu, Jeannine C Muller-Greven, Robert B Moffett filed Critical Usb Corp
Priority to EP05764400A priority Critical patent/EP1778866B1/en
Priority to JP2007519541A priority patent/JP5020074B2/en
Priority to AT05764400T priority patent/ATE527380T1/en
Priority to CA002569394A priority patent/CA2569394A1/en
Publication of WO2006005074A2 publication Critical patent/WO2006005074A2/en
Priority to NO20065470A priority patent/NO20065470L/en
Publication of WO2006005074A3 publication Critical patent/WO2006005074A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Abstract

Methods and compositions for performing nucleic acid duplication and amplification reactions are provided. A single-stranded nucleic acid binding protein is selected and provided in the reaction mixture which is assembled at a low, nonstringent temperature to include all of the necessary reagents for successful nucleic acid duplication or amplification reactions. By incorporating a single-stranded nucleic acid binding protein into the reaction mixture at low temperature, the generation of nonspecific products such as amplification products is improved despite the reaction mixture having been fully assembled at a nonstringent temperature.
PCT/US2005/023824 2004-06-30 2005-06-30 Novel hot start nucleic acid amplification WO2006005074A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP05764400A EP1778866B1 (en) 2004-06-30 2005-06-30 Novel hot start nucleic acid amplification
JP2007519541A JP5020074B2 (en) 2004-06-30 2005-06-30 New hot start nucleic acid amplification method
AT05764400T ATE527380T1 (en) 2004-06-30 2005-06-30 NEW HOT START NUCLEIC ACID AMPLIFICATION
CA002569394A CA2569394A1 (en) 2004-06-30 2005-06-30 Novel hot start nucleic acid amplification
NO20065470A NO20065470L (en) 2004-06-30 2006-11-28 New hot start nucleic acid amplification.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US58436204P 2004-06-30 2004-06-30
US60/584,362 2004-06-30

Publications (2)

Publication Number Publication Date
WO2006005074A2 WO2006005074A2 (en) 2006-01-12
WO2006005074A3 true WO2006005074A3 (en) 2008-06-19

Family

ID=35783411

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/023824 WO2006005074A2 (en) 2004-06-30 2005-06-30 Novel hot start nucleic acid amplification

Country Status (7)

Country Link
US (3) US7700281B2 (en)
EP (2) EP2366802A1 (en)
JP (1) JP5020074B2 (en)
AT (1) ATE527380T1 (en)
CA (1) CA2569394A1 (en)
NO (1) NO20065470L (en)
WO (1) WO2006005074A2 (en)

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US10400280B2 (en) 2012-08-14 2019-09-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
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US20160122817A1 (en) 2014-10-29 2016-05-05 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequencing
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SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP3262188B1 (en) 2015-02-24 2021-05-05 10X Genomics, Inc. Methods for targeted nucleic acid sequence coverage
EP3262407B1 (en) 2015-02-24 2023-08-30 10X Genomics, Inc. Partition processing methods and systems
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US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
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US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (en) 2017-05-26 2023-05-05 10X基因组学有限公司 Single cell analysis of transposase accessibility chromatin
KR20200016259A (en) 2017-06-07 2020-02-14 다카라 바이오 가부시키가이샤 Preservation method of oligonucleotide
EP3645710A1 (en) 2017-06-26 2020-05-06 Thermo Fisher Scientific Baltics Uab Thermophilic dna polymerase mutants
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KR102529958B1 (en) * 2021-01-18 2023-05-08 가톨릭대학교 산학협력단 PCR additive composition for quantitative amplification of low GC content DNA
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Also Published As

Publication number Publication date
JP2008516584A (en) 2008-05-22
EP1778866A4 (en) 2009-06-03
US7951534B2 (en) 2011-05-31
CA2569394A1 (en) 2006-01-12
US20080138878A1 (en) 2008-06-12
WO2006005074A2 (en) 2006-01-12
NO20065470L (en) 2007-01-26
EP1778866B1 (en) 2011-10-05
EP1778866A2 (en) 2007-05-02
US7700281B2 (en) 2010-04-20
US8404443B2 (en) 2013-03-26
JP5020074B2 (en) 2012-09-05
EP2366802A1 (en) 2011-09-21
US20060029952A1 (en) 2006-02-09
ATE527380T1 (en) 2011-10-15
US20110189736A1 (en) 2011-08-04

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