WO2006044646A3 - Pufa polyketide synthase systems and uses thereof - Google Patents

Pufa polyketide synthase systems and uses thereof Download PDF

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Publication number
WO2006044646A3
WO2006044646A3 PCT/US2005/036998 US2005036998W WO2006044646A3 WO 2006044646 A3 WO2006044646 A3 WO 2006044646A3 US 2005036998 W US2005036998 W US 2005036998W WO 2006044646 A3 WO2006044646 A3 WO 2006044646A3
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WIPO (PCT)
Prior art keywords
pufa
systems
polyketide synthase
microorganisms
pks
Prior art date
Application number
PCT/US2005/036998
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French (fr)
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WO2006044646A2 (en
Inventor
Craig A Weaver
Ross Zirkle
James G Metz
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Martek Biosciences Corp
Craig A Weaver
Ross Zirkle
James G Metz
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Publication date
Application filed by Martek Biosciences Corp, Craig A Weaver, Ross Zirkle, James G Metz filed Critical Martek Biosciences Corp
Priority to CA002584004A priority Critical patent/CA2584004A1/en
Priority to BRPI0516327-7A priority patent/BRPI0516327A/en
Priority to EP05816098A priority patent/EP1805315A4/en
Priority to MX2007004502A priority patent/MX2007004502A/en
Priority to JP2007536936A priority patent/JP2008515461A/en
Priority to AU2005295598A priority patent/AU2005295598A1/en
Publication of WO2006044646A2 publication Critical patent/WO2006044646A2/en
Priority to IL182548A priority patent/IL182548A0/en
Publication of WO2006044646A3 publication Critical patent/WO2006044646A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/62—Carboxylic acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]

Abstract

Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from the bacterial microorganisms Shewanella japonica and Shewanella olleyana, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.
PCT/US2005/036998 2004-10-13 2005-10-13 Pufa polyketide synthase systems and uses thereof WO2006044646A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CA002584004A CA2584004A1 (en) 2004-10-13 2005-10-13 Pufa polyketide synthase systems and uses thereof
BRPI0516327-7A BRPI0516327A (en) 2004-10-13 2005-10-13 puffed polyketide synthase systems and their uses
EP05816098A EP1805315A4 (en) 2004-10-13 2005-10-13 Pufa polyketide synthase systems and uses thereof
MX2007004502A MX2007004502A (en) 2004-10-13 2005-10-13 Pufa polyketide synthase systems and uses thereof.
JP2007536936A JP2008515461A (en) 2004-10-13 2005-10-13 PUFA polyketide synthase system and methods of use thereof
AU2005295598A AU2005295598A1 (en) 2004-10-13 2005-10-13 PUFA polyketide synthase systems and uses thereof
IL182548A IL182548A0 (en) 2004-10-13 2007-04-12 Pufa polyketide synthase systems and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/965,017 2004-10-13
US10/965,017 US7217856B2 (en) 1999-01-14 2004-10-13 PUFA polyketide synthase systems and uses thereof

Publications (2)

Publication Number Publication Date
WO2006044646A2 WO2006044646A2 (en) 2006-04-27
WO2006044646A3 true WO2006044646A3 (en) 2009-04-16

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Country Status (11)

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US (15) US7217856B2 (en)
EP (1) EP1805315A4 (en)
JP (1) JP2008515461A (en)
KR (1) KR20070084187A (en)
CN (1) CN101437950A (en)
AU (1) AU2005295598A1 (en)
BR (1) BRPI0516327A (en)
CA (1) CA2584004A1 (en)
IL (1) IL182548A0 (en)
MX (1) MX2007004502A (en)
WO (1) WO2006044646A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753810B (en) * 2018-05-22 2021-06-18 昆明理工大学 Application of transcriptional regulatory protein gene ORF2

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US7247461B2 (en) * 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US7217856B2 (en) * 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7211418B2 (en) * 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7271315B2 (en) * 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7208590B2 (en) * 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
CN103451246B (en) 2004-04-22 2018-02-16 联邦科学技术研究组织 With recombinant cell synthesis of long-chain polyunsaturated fatty acids
ES2715640T3 (en) 2004-04-22 2019-06-05 Commw Scient Ind Res Org Synthesis of long chain polyunsaturated fatty acids by recombinant cells
US8034391B2 (en) 2005-07-01 2011-10-11 Martek Biosciences Corporation Polyunsaturated fatty acid-containing oil product and uses and production thereof
CN101573451B (en) * 2006-03-15 2014-04-30 Dsmip资产公司 Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
MX339812B (en) * 2006-03-15 2016-06-08 Dsm Ip Assets B V * Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems.
MX295615B (en) * 2006-04-11 2012-02-07 Market Biosciences Corp Food products comprising long chain polyunsaturated fatty acids and methods for preparing the same.
AU2007260873B2 (en) 2006-06-22 2013-05-02 Dsm Ip Assets B.V. Encapsulated labile compound compositions and methods of making the same
WO2008025068A1 (en) 2006-08-29 2008-03-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of fatty acids
MX2009002285A (en) 2006-08-29 2011-10-11 Market Biosciences Corp USE OF DPA(n-6) OILS IN INFANT FORMULA.
US20080233628A1 (en) * 2006-09-14 2008-09-25 The Salk Institute For Biological Studies Incorporation of type III polyketide synthases into multidomain proteins of the type I and III polyketide synthase and fatty acid synthase families
AU2008269989B2 (en) * 2007-06-29 2014-02-27 Dsm Ip Assets B.V. Production and purification of esters of polyunsaturated fatty acids
JP5110511B2 (en) * 2007-09-05 2012-12-26 独立行政法人産業技術総合研究所 Method for producing highly unsaturated fatty acids and highly unsaturated lipids using microorganisms
US20110076379A1 (en) * 2008-06-13 2011-03-31 Means Michael M High omega saturated fat free meat products
CA3082380A1 (en) 2008-11-18 2010-05-27 Commonwealth Scientific And Industrial Research Organisation Recombinant cells comprisng exogenous .delta.6 desaturase and methods for producing oil containing unsaturated fatty acids
US20120100550A1 (en) * 2009-03-04 2012-04-26 University Of Miyazaki Method for determining fatty acid synthesis pathway of microorganism, and pcr primer set for use in the method
AU2010225930B2 (en) 2009-03-16 2017-01-12 Dsm Ip Assets B.V. Protein production in microorganisms of the phylum Labyrinthulomycota
ES2717102T3 (en) 2009-03-19 2019-06-19 Dsm Ip Assets Bv Nucleic acid molecules of polyunsaturated fatty acid synthases, and polypeptides, compositions and methods of preparation and uses thereof
WO2011090730A1 (en) * 2009-12-28 2011-07-28 Martek Biosciences Corporation Recombinant thraustochytrids that grow on xylose, and compositions, methods of making, and uses thereof
TW201144442A (en) 2010-05-17 2011-12-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
WO2012021703A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company A sustainable aquaculture feeding strategy
US20120040076A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Aquaculture feed compositions
WO2012021686A1 (en) 2010-08-11 2012-02-16 E. I. Du Pont De Nemours And Company Improved aquaculture meat products
CN106434392A (en) * 2010-10-01 2017-02-22 国立大学法人九州大学 Method for transformation of stramenopile
WO2012135535A1 (en) * 2011-03-31 2012-10-04 Los Alamos National Security, Llc Method of identifying soluble proteins and soluble protein complexes
TW201307553A (en) 2011-07-26 2013-02-16 Dow Agrosciences Llc Production of DHA and other LC-PUFAs in plants
US10385327B2 (en) * 2012-01-05 2019-08-20 University Of Puerto Rico Enhanced E. coli for the production of fatty acids and method of producing the same
EP3266316A1 (en) 2012-06-15 2018-01-10 Commonwealth Scientific and Industrial Research Organisation Production of long chain polyunsaturated fatty acids in plant cells
KR20150040359A (en) 2012-08-10 2015-04-14 오피엑스 바이오테크놀로지스, 인크. Microorganisms and methods for the production of fatty acids and fatty acid derived products
US9809804B2 (en) 2013-01-23 2017-11-07 Iowa State University Research Foundation, Inc. Materials and methods for characterizing and using KASIII for production of bi-functional fatty acids
US10184140B2 (en) 2013-01-23 2019-01-22 Iowa State University Research Foundation, Inc. Materials and methods for production of bi-functional fatty acids in recombinant bacteria
US20150057465A1 (en) 2013-03-15 2015-02-26 Opx Biotechnologies, Inc. Control of growth-induction-production phases
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
JP6603658B2 (en) * 2013-07-19 2019-11-06 カーギル インコーポレイテッド Microorganisms and methods for the production of fatty acids and fatty acid derivatives
EP2843043A1 (en) * 2013-08-27 2015-03-04 Evonik Industries AG A method for producing acyl amino acids
US9580758B2 (en) 2013-11-12 2017-02-28 Luc Montagnier System and method for the detection and treatment of infection by a microbial agent associated with HIV infection
TW201525136A (en) 2013-11-26 2015-07-01 Dow Agrosciences Llc Production of omega-3 long-chain polyunsaturated fatty acids in oilseed crops by a thraustochytrid PUFA synthase
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
MX2016008270A (en) 2013-12-18 2017-05-26 Commw Scient Ind Res Org Lipid comprising long chain polyunsaturated fatty acids.
WO2015116671A2 (en) 2014-01-28 2015-08-06 Dsm Ip Assets B.V. Factors for the production and accumulation of polyunsaturated fatty acids (pufas) derived from pufa synthases
WO2015134547A1 (en) 2014-03-03 2015-09-11 Synthetic Genomics, Inc. Molecules associated with fatty acid biosynthetic pathways and uses thereof
WO2015172119A1 (en) * 2014-05-08 2015-11-12 Scfm Ventures, Llc Method for optimizing production of eicosapentaenoic acid (epa) ina recombinant host
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
CN105567715B (en) * 2014-10-17 2021-06-29 丰益(上海)生物技术研发中心有限公司 Schizochytrium limacinum alpha-tubulin related sequence and application thereof
EP3265567B1 (en) 2015-03-02 2020-07-29 Conagen Inc. Regulatory elements from labyrinthulomycetes microorganisms
CN105176942A (en) * 2015-08-04 2015-12-23 北京农学院 Polyketide synthase RinPKS1 derived from raspberry and related biomaterials and application thereof
KR20180061081A (en) 2015-10-01 2018-06-07 디에스엠 아이피 어셋츠 비.브이. Supplement material for use in pet food
AU2017235543B2 (en) 2016-03-16 2020-05-14 Conagen, Inc. Production of proteins in labyrinthulomycetes
WO2017194683A1 (en) 2016-05-12 2017-11-16 Dsm Ip Assets B.V. Method of increasing omega-3 polyunsaturated fatty acids production in microalgae
CN106591388A (en) * 2016-10-13 2017-04-26 中国农业科学院生物技术研究所 Gene Dr2091 having synthesis function of polyketone compounds
CN110418642A (en) * 2016-10-28 2019-11-05 银杏生物制品公司 For producing the composition and method of compound
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
BR112021002299A2 (en) * 2018-08-10 2021-05-04 Kyowa Hakko Bio Co., Ltd microorganism producing polyunsaturated fatty acid and method for producing polyunsaturated fatty acid
BR112021002300A2 (en) * 2018-08-10 2021-05-04 Kyowa Hakko Bio Co., Ltd microorganism that produces eicosapentaenoic acid and method for producing eicosapentaenoic acid
WO2023070027A2 (en) * 2021-10-20 2023-04-27 University Of Puerto Rico Fatty acid production in escherichia coli

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683898A (en) * 1992-05-15 1997-11-04 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
US5798259A (en) * 1992-05-15 1998-08-25 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
WO1998055625A1 (en) * 1997-06-04 1998-12-10 Calgene, Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
US20030166207A1 (en) * 2001-04-16 2003-09-04 Roessler Paul G. Product and process for transformation of thraustochytriales microorganisms

Family Cites Families (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US674574A (en) * 1900-08-01 1901-05-21 Charles Decrette Metal-bending machine.
US781861A (en) * 1903-11-07 1905-02-07 Alonzo W Allen Thread or yarn tension device.
US778594A (en) * 1904-06-29 1904-12-27 Pancrazio M Megaro Shaving composition.
US777277A (en) * 1904-09-07 1904-12-13 American Clay Working Machinery Company Tile-press.
US781882A (en) * 1904-11-22 1905-02-07 William Hinckle Smith Compressed product from bone.
US5246841A (en) 1986-12-26 1993-09-21 Sagami Chemical Research Center Microbial process for production of eicosapentaenoic acid
US5130242A (en) * 1988-09-07 1992-07-14 Phycotech, Inc. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids
US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US5639790A (en) 1991-05-21 1997-06-17 Calgene, Inc. Plant medium-chain thioesterases
EP0594868A4 (en) 1992-05-15 1997-01-02 Sagami Chem Res Gene which codes for eicosapentaenoic acid synthetase group and process for producing eicosapentaenoic acid
US5310242A (en) 1992-09-28 1994-05-10 Golder Kimberly A Portable infant seat
DE4323727A1 (en) 1993-07-15 1995-03-09 Boehringer Mannheim Gmbh Method of identifying human and animal cells capable of unlimited proliferation or tumor formation
US5672491A (en) 1993-09-20 1997-09-30 The Leland Stanford Junior University Recombinant production of novel polyketides
AU4400196A (en) 1995-01-13 1996-07-31 Sagami Chemical Research Center Genes coding for biosynthetic enzyme group for icosapentaenoic acid and process for producing icosapentaenoic acid
EP0823475B1 (en) 1995-04-17 2009-06-17 National Institute of Advanced Industrial Science and Technology Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
EP0913473A4 (en) 1996-07-10 2000-12-06 Sagami Chem Res Process for producing icosapentaenoic acid by genetic recombination
US6033883A (en) 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
EP0970044B1 (en) * 1997-03-14 2003-05-07 Basf Aktiengesellschaft Cycloalkylalkanecarboxamides and the production and use thereof
JP4087460B2 (en) 1997-04-11 2008-05-21 カルジーン エル エル シー Methods and compositions for the synthesis of long chain polyunsaturated fatty acids in plants
US6566583B1 (en) * 1997-06-04 2003-05-20 Daniel Facciotti Schizochytrium PKS genes
US6626260B2 (en) * 1998-04-09 2003-09-30 Bombardier Inc. All terrain vehicle
US6677145B2 (en) 1998-09-02 2004-01-13 Abbott Laboratories Elongase genes and uses thereof
US7211418B2 (en) 1999-01-14 2007-05-01 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US8003772B2 (en) * 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
US7217856B2 (en) 1999-01-14 2007-05-15 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US7271315B2 (en) 1999-01-14 2007-09-18 Martek Biosciences Corporation PUFA polyketide synthase systems and uses thereof
US20070244192A1 (en) * 1999-01-14 2007-10-18 Martek Biosciences Corporation Plant seed oils containing polyunsaturated fatty acids
US7247461B2 (en) 1999-01-14 2007-07-24 Martek Biosciences Corporation Nucleic acid molecule encoding ORFA of a PUFA polyketide synthase system and uses thereof
US6246841B1 (en) * 2000-05-10 2001-06-12 Lexmark International, Inc. Removable toner cartridge
AU6829601A (en) 2000-06-08 2001-12-17 Univ Miami Fatty acid elongase 3-ketoacyl coa synthase polypeptides
US20040010817A1 (en) * 2000-07-21 2004-01-15 Washington State University Research Foundation Plant acyl-CoA synthetases
ATE510908T1 (en) * 2000-09-28 2011-06-15 Bioriginal Food & Science Corp FAD5-2, MEMBER OF THE DESATURASE FAMILY AND USES THEREOF
TWI426126B (en) 2001-04-16 2014-02-11 Dsm Ip Assets Bv Pufa polyketide synthase systems and uses thereof
US20040005672A1 (en) 2002-02-22 2004-01-08 Santi Daniel V. Heterologous production of polyketides
GB2385852A (en) * 2002-02-27 2003-09-03 Rothamsted Ex Station Delta 6-desaturases from Primulaceae
ATE495263T1 (en) 2002-03-16 2011-01-15 Univ York TRANSGENIC PLANTS THAT EXPRESS FATTY ACID BIOSYNTHESIS ENZYMES
US7089432B2 (en) * 2002-12-27 2006-08-08 Matsushita Electric Industrial Co., Ltd. Method for operating a processor at first and second rates depending upon whether the processor is executing code to control predetermined hard drive operations
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
KR101234200B1 (en) 2003-03-26 2013-02-19 마텍 바이오싸이언스스 코포레이션 Pufa polyketide synthase systems and uses thereof
CA2868312A1 (en) 2003-03-31 2004-10-14 University Of Bristol Novel plant acyltransferases specific for long-chained, multiply unsaturated fatty acids
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7208590B2 (en) 2003-07-15 2007-04-24 Abbott Laboratories Genes involved in polyketide synthase pathways and uses thereof
WO2005078010A1 (en) * 2004-02-17 2005-08-25 Toray Industries, Inc. Biaxially oriented polyester film
DE102004060340A1 (en) 2004-07-16 2006-02-09 Basf Plant Science Gmbh Method for increasing the content of polyunsaturated long-chain fatty acids in transgenic organisms
AR050881A1 (en) 2004-09-20 2006-11-29 Basf Plant Science Gmbh ARABIDOPSIS GENES THAT CODIFY PROTEINS PARTICIPATING IN THE METABOLISM OF SUGARS AND LIPIDS AND METHODS OF USE
MX339812B (en) * 2006-03-15 2016-06-08 Dsm Ip Assets B V * Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems.
US20080044784A1 (en) * 2006-08-15 2008-02-21 Park Keith K Multi-component lighter
US7669708B2 (en) * 2006-08-31 2010-03-02 Martin Engineering Company Bulk material handling system and control

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5683898A (en) * 1992-05-15 1997-11-04 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
US5798259A (en) * 1992-05-15 1998-08-25 Sagami Chemical Research Center Gene coding for eicosapentaenoic acid synthesizing enzymes and process for production of eicosapentaenoic acid
WO1998055625A1 (en) * 1997-06-04 1998-12-10 Calgene, Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
US6140486A (en) * 1997-06-04 2000-10-31 Calgene Llc Production of polyunsaturated fatty acids by expression of polyketide-like synthesis genes in plants
US20030166207A1 (en) * 2001-04-16 2003-09-04 Roessler Paul G. Product and process for transformation of thraustochytriales microorganisms

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
ALLEN ET AL.: "Structure and regulation of the omega-3 polyunsaturated fatty acid synthase genes from the deep-sea bactrium Photobacterium profundum strain SS9.", MICROBIOLOGY., vol. 148, 2002, pages 1903 - 1913, XP002502217 *
ORIKASA ET AL.: "Characterization of the eicosapentaenoic acid biosynthesis gene cluster from SHEWANELLA sp. strain SCRC-2738", CELLULAR AND MOLECULAR BIOLOGY., vol. 50, no. 5, July 2004 (2004-07-01), pages 625 - 630, XP009130053 *
SATOMI ET AL.: "Shewanella marinintesina sp. nov., Shewanella schlegeliana sp. nov. and Shewanella sairae sp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from see-animal intestines.", INTERNAT. J. SYST. EVOL. MICROBIOL., vol. 53, 2003, pages 491 - 499, XP003025842 *
SINGH ET AL.: "Microbial production of docosahexaenoic acid (DHA, C22:6).", ADV. APPL. MICROBIOL., vol. 45, 1997, pages 271 - 312, XP008046097 *
TAKEYAMA ET AL.: "Expression of eicosapentaenoic acid synthesis gene clustter from Shewanella sp. in transgenic marine cyanobacterium, Synechecoccus sp.", MICROBIOLOGY., vol. 143, 1997, pages 2725 - 2731, XP000952940 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108753810B (en) * 2018-05-22 2021-06-18 昆明理工大学 Application of transcriptional regulatory protein gene ORF2

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