WO2006091942A3 - Methods of expressing, purifying and characterizing concanavalin a, mutants thereof, and sensors including the same - Google Patents

Methods of expressing, purifying and characterizing concanavalin a, mutants thereof, and sensors including the same Download PDF

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Publication number
WO2006091942A3
WO2006091942A3 PCT/US2006/006873 US2006006873W WO2006091942A3 WO 2006091942 A3 WO2006091942 A3 WO 2006091942A3 US 2006006873 W US2006006873 W US 2006006873W WO 2006091942 A3 WO2006091942 A3 WO 2006091942A3
Authority
WO
WIPO (PCT)
Prior art keywords
purifying
concanavalin
characterizing
expressing
methods
Prior art date
Application number
PCT/US2006/006873
Other languages
French (fr)
Other versions
WO2006091942A2 (en
Inventor
Stephen J Palmieri
Dylan A Bulseco
Edward R Appelbaum
Original Assignee
Lifescan Inc
Stephen J Palmieri
Dylan A Bulseco
Edward R Appelbaum
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lifescan Inc, Stephen J Palmieri, Dylan A Bulseco, Edward R Appelbaum filed Critical Lifescan Inc
Publication of WO2006091942A2 publication Critical patent/WO2006091942A2/en
Publication of WO2006091942A3 publication Critical patent/WO2006091942A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/42—Lectins, e.g. concanavalin, phytohaemagglutinin

Abstract

A novel method for purifying various lectins is disclosed. More specifically a novel method for purifying Concanavalin A is set forth. Methods of expressing purifying and characterizing a mutant Concanavalin A, and sensors including the foregoing are also disclosed.
PCT/US2006/006873 2005-02-24 2006-02-24 Methods of expressing, purifying and characterizing concanavalin a, mutants thereof, and sensors including the same WO2006091942A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65575605P 2005-02-24 2005-02-24
US60/655,756 2005-02-24

Publications (2)

Publication Number Publication Date
WO2006091942A2 WO2006091942A2 (en) 2006-08-31
WO2006091942A3 true WO2006091942A3 (en) 2008-06-05

Family

ID=36928114

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/006873 WO2006091942A2 (en) 2005-02-24 2006-02-24 Methods of expressing, purifying and characterizing concanavalin a, mutants thereof, and sensors including the same

Country Status (1)

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WO (1) WO2006091942A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060247154A1 (en) * 2005-02-24 2006-11-02 Lifescan, Inc. Concanavalin a, methods of expressing, purifying and characterizing concanavalina, and sensors including the same
WO2010107520A1 (en) * 2009-03-20 2010-09-23 Smartcells, Inc. Soluble non-depot insulin conjugates and uses thereof
KR101673195B1 (en) * 2013-11-18 2016-11-07 한국생명공학연구원 The Recombinant Sialic Acid-Specific Binding Lectin, PSL1b, from the Mushroom Polyporus squamosus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889842A (en) * 1987-03-03 1989-12-26 Morris Randall E Concanavalin A dimers as therapeutic agents
US5342789A (en) * 1989-12-14 1994-08-30 Sensor Technologies, Inc. Method and device for detecting and quantifying glucose in body fluids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889842A (en) * 1987-03-03 1989-12-26 Morris Randall E Concanavalin A dimers as therapeutic agents
US5342789A (en) * 1989-12-14 1994-08-30 Sensor Technologies, Inc. Method and device for detecting and quantifying glucose in body fluids

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
STUBBS ET AL.: "Production of Pea Lectin in Escherichia coli", J. BIOL. CHEM., vol. 261, no. 14, May 1986 (1986-05-01), pages 6141 - 6144, XP002914631 *

Also Published As

Publication number Publication date
WO2006091942A2 (en) 2006-08-31

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