WO2006107506A3 - System and method for performing g protein coupled receptor (gpcr) cell assays using waveguide-grating sensors - Google Patents

System and method for performing g protein coupled receptor (gpcr) cell assays using waveguide-grating sensors Download PDF

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Publication number
WO2006107506A3
WO2006107506A3 PCT/US2006/008582 US2006008582W WO2006107506A3 WO 2006107506 A3 WO2006107506 A3 WO 2006107506A3 US 2006008582 W US2006008582 W US 2006008582W WO 2006107506 A3 WO2006107506 A3 WO 2006107506A3
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WO
WIPO (PCT)
Prior art keywords
gpcr
present
different ways
living cells
extended embodiment
Prior art date
Application number
PCT/US2006/008582
Other languages
French (fr)
Other versions
WO2006107506A2 (en
Inventor
Ye Fang
Ann M Ferrie
Norman H Fontaine
Po Ki Yuen
Joydeep Lahiri
Original Assignee
Corning Inc
Ye Fang
Ann M Ferrie
Norman H Fontaine
Po Ki Yuen
Joydeep Lahiri
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Corning Inc, Ye Fang, Ann M Ferrie, Norman H Fontaine, Po Ki Yuen, Joydeep Lahiri filed Critical Corning Inc
Priority to EP06737733.3A priority Critical patent/EP1882186B1/en
Priority to JP2008505316A priority patent/JP2008536117A/en
Publication of WO2006107506A2 publication Critical patent/WO2006107506A2/en
Publication of WO2006107506A3 publication Critical patent/WO2006107506A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5041—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects involving analysis of members of signalling pathways
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/47—Scattering, i.e. diffuse reflection
    • G01N21/4788—Diffraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/55—Specular reflectivity
    • G01N21/552—Attenuated total reflection
    • G01N21/553—Attenuated total reflection and using surface plasmons
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G01N2333/72—Assays involving receptors, cell surface antigens or cell surface determinants for hormones
    • G01N2333/726—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Abstract

The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.
PCT/US2006/008582 2005-04-05 2006-03-10 System and method for performing g protein coupled receptor (gpcr) cell assays using waveguide-grating sensors WO2006107506A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP06737733.3A EP1882186B1 (en) 2005-04-05 2006-03-10 System and method for performing g protein coupled receptor (gpcr) cell assays using waveguide-grating sensors
JP2008505316A JP2008536117A (en) 2005-04-05 2006-03-10 Method and system for performing G protein-coupled receptor (GPCR) cell analysis using a waveguide grating sensor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/100,262 2005-04-05
US11/100,262 US20060223051A1 (en) 2005-04-05 2005-04-05 System and method for performing G protein coupled receptor (GPCR) cell assays using waveguide-grating sensors

Publications (2)

Publication Number Publication Date
WO2006107506A2 WO2006107506A2 (en) 2006-10-12
WO2006107506A3 true WO2006107506A3 (en) 2006-11-16

Family

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Family Applications (1)

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PCT/US2006/008582 WO2006107506A2 (en) 2005-04-05 2006-03-10 System and method for performing g protein coupled receptor (gpcr) cell assays using waveguide-grating sensors

Country Status (4)

Country Link
US (2) US20060223051A1 (en)
EP (1) EP1882186B1 (en)
JP (1) JP2008536117A (en)
WO (1) WO2006107506A2 (en)

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Also Published As

Publication number Publication date
US8148092B2 (en) 2012-04-03
JP2008536117A (en) 2008-09-04
EP1882186A2 (en) 2008-01-30
WO2006107506A2 (en) 2006-10-12
EP1882186B1 (en) 2014-10-01
US20090275074A1 (en) 2009-11-05
US20060223051A1 (en) 2006-10-05

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