WO2007030759A3 - Improved nucleic acid amplification procedure - Google Patents

Improved nucleic acid amplification procedure Download PDF

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Publication number
WO2007030759A3
WO2007030759A3 PCT/US2006/035154 US2006035154W WO2007030759A3 WO 2007030759 A3 WO2007030759 A3 WO 2007030759A3 US 2006035154 W US2006035154 W US 2006035154W WO 2007030759 A3 WO2007030759 A3 WO 2007030759A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
methods
acid amplification
amplification procedure
improved nucleic
Prior art date
Application number
PCT/US2006/035154
Other languages
French (fr)
Other versions
WO2007030759A2 (en
Inventor
Nurith Kurn
Shenglong Wang
Original Assignee
Nugen Technologies Inc
Nurith Kurn
Shenglong Wang
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nugen Technologies Inc, Nurith Kurn, Shenglong Wang filed Critical Nugen Technologies Inc
Priority to EP06803269A priority Critical patent/EP1929046B1/en
Priority to CA002621267A priority patent/CA2621267A1/en
Priority to US12/091,843 priority patent/US7939258B2/en
Publication of WO2007030759A2 publication Critical patent/WO2007030759A2/en
Publication of WO2007030759A3 publication Critical patent/WO2007030759A3/en
Priority to US13/103,865 priority patent/US8852867B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Abstract

The invention provides methods for amplification of polynucleotide sequences using primers containing single-stranded RNA. The methods employ use of an enzyme capable of cleaving single-stranded RNA, such as RNase I, to degrade a first RNA- containing primer prior to addition of a second RNA-containing primer. The invention also provides compositions and kits for practicing the amplification methods, as well as methods which use the amplification products.
PCT/US2006/035154 2005-09-07 2006-09-07 Improved nucleic acid amplification procedure WO2007030759A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP06803269A EP1929046B1 (en) 2005-09-07 2006-09-07 Improved nucleic acid amplification procedure
CA002621267A CA2621267A1 (en) 2005-09-07 2006-09-07 Improved nucleic acid amplification procedure
US12/091,843 US7939258B2 (en) 2005-09-07 2006-09-07 Nucleic acid amplification procedure using RNA and DNA composite primers
US13/103,865 US8852867B2 (en) 2005-09-07 2011-05-09 Nucleic acid amplification procedure using RNA and DNA composite primers

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US71496605P 2005-09-07 2005-09-07
US60/714,966 2005-09-07

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/091,843 A-371-Of-International US7939258B2 (en) 2005-09-07 2006-09-07 Nucleic acid amplification procedure using RNA and DNA composite primers
US13/103,865 Continuation US8852867B2 (en) 2005-09-07 2011-05-09 Nucleic acid amplification procedure using RNA and DNA composite primers

Publications (2)

Publication Number Publication Date
WO2007030759A2 WO2007030759A2 (en) 2007-03-15
WO2007030759A3 true WO2007030759A3 (en) 2007-06-21

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/035154 WO2007030759A2 (en) 2005-09-07 2006-09-07 Improved nucleic acid amplification procedure

Country Status (4)

Country Link
US (2) US7939258B2 (en)
EP (1) EP1929046B1 (en)
CA (1) CA2621267A1 (en)
WO (1) WO2007030759A2 (en)

Cited By (9)

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US7771946B2 (en) 2001-03-09 2010-08-10 Nugen Technologies, Inc. Methods, kits and compositions for single primer linear isothermal amplification of nucleic acid sequences
US7771934B2 (en) 2000-12-13 2010-08-10 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US8143001B2 (en) 2003-12-29 2012-03-27 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8465950B2 (en) 2003-04-14 2013-06-18 Nugen Technologies, Inc. Global amplification using a randomly primed composite primer
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing

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US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
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