WO2007070542A3 - Nanoprobes for detection or modification of molecules - Google Patents

Nanoprobes for detection or modification of molecules Download PDF

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Publication number
WO2007070542A3
WO2007070542A3 PCT/US2006/047456 US2006047456W WO2007070542A3 WO 2007070542 A3 WO2007070542 A3 WO 2007070542A3 US 2006047456 W US2006047456 W US 2006047456W WO 2007070542 A3 WO2007070542 A3 WO 2007070542A3
Authority
WO
WIPO (PCT)
Prior art keywords
functional groups
target
target biomolecule
molecules
probes
Prior art date
Application number
PCT/US2006/047456
Other languages
French (fr)
Other versions
WO2007070542A2 (en
Inventor
Ilya G Lyakhov
Thomas D Schneider
Danielle Needle
Original Assignee
Us Gov Health & Human Serv
Ilya G Lyakhov
Thomas D Schneider
Danielle Needle
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv, Ilya G Lyakhov, Thomas D Schneider, Danielle Needle filed Critical Us Gov Health & Human Serv
Publication of WO2007070542A2 publication Critical patent/WO2007070542A2/en
Publication of WO2007070542A3 publication Critical patent/WO2007070542A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
    • Y10T436/143333—Saccharide [e.g., DNA, etc.]

Abstract

The disclosure provides probes for one or more target molecules. In particular examples, the probes include a molecular linker and first and second functional groups linked and spaced by the molecular linker, wherein the functional groups are capable of interacting with one another or with the target biomolecule in a predetermined reaction, and wherein the molecular linker maintains the first and second functional groups sufficiently spaced from one another such that the functional groups do not substantially interact in an absence of the target biomolecule. In the presence of the target biomolecule the functional groups interact (with each other, with the target biomolecule, or both), and in some examples a detectable signal is produced. In some examples, the functional groups can detect or modify a target molecule. Also provided are methods of using the probes, for example to detect or modify a target molecule.
PCT/US2006/047456 2005-12-12 2006-12-12 Nanoprobes for detection or modification of molecules WO2007070542A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US74985805P 2005-12-12 2005-12-12
US74972905P 2005-12-12 2005-12-12
US60/749,729 2005-12-12
US60/749,858 2005-12-12

Publications (2)

Publication Number Publication Date
WO2007070542A2 WO2007070542A2 (en) 2007-06-21
WO2007070542A3 true WO2007070542A3 (en) 2008-04-03

Family

ID=38051529

Family Applications (2)

Application Number Title Priority Date Filing Date
PCT/US2006/047534 WO2007070572A2 (en) 2005-12-12 2006-12-12 Probe for nucleic acid sequencing and methods of use
PCT/US2006/047456 WO2007070542A2 (en) 2005-12-12 2006-12-12 Nanoprobes for detection or modification of molecules

Family Applications Before (1)

Application Number Title Priority Date Filing Date
PCT/US2006/047534 WO2007070572A2 (en) 2005-12-12 2006-12-12 Probe for nucleic acid sequencing and methods of use

Country Status (6)

Country Link
US (3) US7871777B2 (en)
EP (2) EP1960550B1 (en)
JP (1) JP2009519041A (en)
AT (1) ATE481505T1 (en)
DE (1) DE602006016984D1 (en)
WO (2) WO2007070572A2 (en)

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